FastQCFastQC Report
Sat 23 Apr 2022
EGAF00004684391

Summary

[OK]Basic Statistics

MeasureValue
FilenameEGAF00004684391
File typeConventional base calls
EncodingSanger / Illumina 1.9
Total Sequences797680
Sequences flagged as poor quality0
Sequence length20-76
%GC55

[OK]Per base sequence quality

Per base quality graph

[OK]Per tile sequence quality

Per base quality graph

[OK]Per sequence quality scores

Per Sequence quality graph

[FAIL]Per base sequence content

Per base sequence content

[FAIL]Per sequence GC content

Per sequence GC content graph

[OK]Per base N content

N content graph

[WARN]Sequence Length Distribution

Sequence length distribution

[FAIL]Sequence Duplication Levels

Duplication level graph

[WARN]Overrepresented sequences

SequenceCountPercentagePossible Source
GTCCTGGGCCCAGTCTGTGCTGACTCAGCCACCCTCAGCGTCTGGGACCC31970.400787283121051No Hit
GGGTTAGAGTTGGGAACCTATGAACATTCTGTAGGGGCCACTGTCTTCTCCACGGTGCTCCCTTCATGCGTGAC28310.35490422224450907No Hit
CCTCTGAGGAGCTCCAAGCCAACAAGGCCACACTAGTGTGTCTGATCAGT26080.3269481496339384No Hit
GCCCAAGGCCAACCCCACTGTCACTCTGTTCCCGCCCTCCTCTGAGGAGC25590.3208053354728713No Hit
GGGGGGGGGGGGGGGGGGGGGGGGGGGGGGGGGGGGGGGGGGGGGGGGGG24500.30714070805335475No Hit
GTGCAGGGTCCTGGGCCCAGTCTGTGCTGACTCAGCCACCCTCAGCGTCT24080.30187543877244005No Hit
GTTTGGAGGGTTTGGTGGTCTCCACTCCCGCCTTGACGGGGCTGCCATCTGCCTTCCAGGCCACTGTCACAGCTC23670.29673553304583294No Hit
CCTCAGAGGAGGGCGGGAACAGAGTGACAGTGGGGTTGGCCTTGGGCTGA22750.28520208604954367No Hit
CACTAGTGTGGCCTTGTTGGCTTGGAGCTCCTCAGAGGAGGGCGGGAACAGAGTGACAGTGGGGTTGGCCTTGGG22300.27956072610570654No Hit
GTGCTGACTCAGCCACCCTCAGCGTCTGGGACCCCCGGGCAGAGCGTCAC20190.2531090161468258No Hit
GTGCAGGGAGAAGGGCTGGATGACTTGGGATGGGGAGAGAGACCCCTCCCCTGGGATCCTGCAGCTCCAGGCTCC20070.2516046534951359No Hit
GTCCTGGGTCAGCCCAAGGCCAACCCCACTGTCACTCTGTTCCCGCCCTC20000.25072710861498343No Hit
GTATACATAGTTCCTCCCGATGTTGGAGCTGTTTCCAAAACAAGAGATGGTGACGCTCTGCCCGGGGGTCCCAGA18710.234555210109317No Hit
CTATGTATACTGGTACCAGCAGTTCCCAGGAACTGCCCCCAAACTCGTCA18500.2319225754688597No Hit
GATTATTATTGTGCAGCGTGGGATGACAGTGGTCGGGGAATCTTCGGAACTGGGACCAAGGTCACCGTCCTGGGT18440.23117039414301474No Hit
ACACTAGTGTGTCTGATCAGTGACTTCTACCCGGGAGCTGTGACAGTGGC17970.22527830709056262No Hit
GCCTTGGGCTGACCCAGGACGGTGACCTTGGTCCCAGTTCCGAAGATTCCCCGACCACTGTCATCCCACGCTGCA17680.2216427640156454No Hit
GTCTTCTCCACGGTGCTCCCTTCATGCGTGACCTGGCAGCTGTAGCTTCT17490.21926085648380306No Hit
GTGGTAGAGAAGACAGGATTCAGGACAATCTCCAGCATGGCCGGCTTCCCTCTCCTCCTCACCCTCCTCACTCA17370.21775649383211312No Hit
CCTCACTCACTGTGCAGGGTCCTGGGCCCAGTCTGTGCTGACTCAGCCAC17180.2153745863002708No Hit
GGATGACAGTGGTCGGGGAATCTTCGGAACTGGGACCAAGGTCACCGTCCTGGGTCAGCCCAAGGCCAACCCCA16900.211864406779661No Hit
GTGCTCCCTTCATGCGTGACCTGGCAGCTGTAGCTTCTGTGGGACTTCCACTGCTCGGGCGTCAGGCTCAGGTAG15430.19343596429645973No Hit
CTATAGATGACGAGTTTGGGGGCAGTTCCTGGGAACTGCTGGTACCAGTATACATAGTTCCTCCCGATGTTGGAG14960.18754387724400762No Hit
CCTATGAACATTCTGTAGGGGCCACTGTCTTCTCCACGGTGCTCCCTTCATGCGTGACCTGGCAGCTGTAGCTT14890.1866663323638552No Hit
GTATCAACGCAGAGTACGGGAGCTTCAACTGTGGTAGAGAAGACAGGATTCAGGACAATCTCCAGCATGGCCGG14790.18541269682078027No Hit
ATGTATACTGGTACCAGCAGTTCCCAGGAACTGCCCCCAAACTCGTCATC14610.1831561528432454No Hit
CTTCATGCGTGACCTGGCAGCTGTAGCTTCTGTGGGACTTCCACTGCTCGGGCGTCAGGCTCAGGTAGCTGCTGG14270.1788937919967907No Hit
GAGAAGGGCTGGATGACTTGGGATGGGGAGAGAGACCCCTCCCCTGGGAT14260.1787684284424832No Hit
GAACTATGTATACTGGTACCAGCAGTTCCCAGGAACTGCCCCCAAACTCGTCATCTATAGTGATCATCAGCGGCC13850.17362852271587606No Hit
ATTCAGGACAATCTCCAGCATGGCCGGCTTCCCTCTCCTCCTCACCCTCCTCACTCACTGTGCAGGGTCCTGGGC13020.16322334770835423No Hit
GAGTGACAGTGGGGTTGGCCTTGGGCTGACCCAGGACGGTGACCTTGGTCCCAGTTCCGAAGATTCCCCGACCA12850.16109216728512685No Hit
GTTTGGTGGTCTCCACTCCCGCCTTGACGGGGCTGCCATCTGCCTTCCAG12700.15921171397051448No Hit
ACCCAGGACGGTGACCTTGGTCCCAGTTCCGAAGATTCCCCGACCACTGTCATCCCACGCTGCACAATAATAATC12480.15645371577574968No Hit
CCCTTCATGCGTGACCTGGCAGCTGTAGCTTCTGTGGGACTTCCACTGCTCGGGCGTCAGGCTCAGGTAGCTG12030.15081235583191255No Hit
GTAATAAGTGGCCGTGTCTTCAGACATCAGACCGTTCAGCTCCATGTAGATTGTATTCGTGGACATGTCGGTGGA11850.1485558118543777No Hit
CATCTATAGTGATCATCAGCGGCCCTCAGACATCCCTGGCCGATTCTCTGGCTCCAAGTCTGGCACCTCAGCCTC11670.14629926787684283No Hit
GGCTCAGGTAGCTGCTGGCCGCGTACTTGTTGTTGCTCTGTTTGGAGGGTTTGGTGGTCTCCACTCCCGCCTTGA11340.1421622705846956No Hit
CCCTCAGCGTCTGGGACCCCCGGGCAGAGCGTCACCATCTCTTGTTTTGGAAACAGCTCCAACATCGGGAGGAA11110.1392789088356233No Hit
GAGTACGGGAGCTTCAACTGTGGTAGAGAAGACAGGATTCAGGACAATCTCCAGCATGGCCGGCTTCCCTCTCCT10960.13739845552101093No Hit
GTTCCCGCCCTCCTCTGAGGAGCTCCAAGCCAACAAGGCCACACTAGTGT10800.13539263865209106No Hit
ACATAGTTCCTCCCGATGTTGGAGCTGTTTCCAAAACAAGAGATGGTGACGCTCTGCCCGGGGGTCCCAGACG10240.12837227961087153No Hit
GTACGGGAGCTTCAACTGTGGTAGAGAAGACAGGATTCAGGACAATCTCCAGCATGGCCGGCTTCCCTCTCCTC9880.12385919165580184No Hit
CTGTCACTCTGTTCCCGCCCTCCTCTGAGGAGCTCCAAGCCAACAAGGCC9780.1226055561127269No Hit
GGATATTTATTGGGGTTTCATGAGTGCAGGGAGAAGGGCTGGATGACTTGGGATGGGGAGAGAGACCCCTCCC9700.12160264767826698No Hit
CTGTCACAGCTCCCGGGTAGAAGTCACTGATCAGACACACTAGTGTGGCC9330.11696419616888977No Hit
GGGATGGGGAGAGAGACCCCTCCCCTGGGATCCTGCAGCTCCAGGCTCCCGTGGGTGGGGTTAGAGTTGGGAAC9260.11608665128873734No Hit
GACTTCCACTGCTCGGGCGTCAGGCTCAGGTAGCTGCTGGCCGCGTACTT9180.1150837428542774No Hit
GACCCAGGACGGTGACCTTGGTCCCAGTTCCGAAGATTCCCCGACCACTGTCATCCCACGCTGCACAATAATAAT9120.11433156152843246No Hit
CTAGTGTGTCTGATCAGTGACTTCTACCCGGGAGCTGTGACAGTGGCCTG9060.11357938020258751No Hit
CCCCTGGGATCCTGCAGCTCCAGGCTCCCGTGGGTGGGGTTAGAGTTGGG9040.11332865309397253No Hit
TTCCAGGCCACTGTCACAGCTCCCGGGTAGAAGTCACTGATCAGACACACTAGTGTGGCCTTGTTGGCTTGGAG8930.11194965399659013No Hit
GGGCTGGATGACTTGGGATGGGGAGAGAGACCCCTCCCCTGGGATCCTGC8870.11119747267074516No Hit
CCCGTGGGTGGGGTTAGAGTTGGGAACCTATGAACATTCTGTAGGGGCCA8840.1108213820078227No Hit
CTCCACTCCCGCCTTGACGGGGCTGCCATCTGCCTTCCAGGCCACTGTCACAGCTCCCGGGTAGAAGTCACTGAT8740.10956774646474776No Hit
TTATTGTGCAGCGTGGGATGACAGTGGTCGGGGAATCTTCGGAACTGGGACCAAGGTCACCGTCCTGGGTCAGCC8710.1091916558018253No Hit
GGCCATCAGTGGGCTCCGGTCCGAGGATGAGGCGGATTATTATTGTGCAGCGTGGGATGACAGTGGTCGGGGAAT8700.10906629224751781No Hit
TCCCTGGCCGATTCTCTGGCTCCAAGTCTGGCACCTCAGCCTCCCTGGCC8660.10856483803028784No Hit
CTTCTCCACGGTGCTCCCTTCATGCGTGACCTGGCAGCTGTAGCTTCTGT8620.10806338381305787No Hit
CTGATGGCCAGGGAGGCTGAGGTGCCAGACTTGGAGCCAGAGAATCGGCC8560.1073112024872129No Hit
AGTGTGGCCTTGTTGGCTTGGAGCTCCTCAGAGGAGGGCGGGAACAGAGTGACAGTGGGGTTGGCCTTGGGCTGA8530.10693511182429044No Hit
ATCTCCAGCATGGCCGGCTTCCCTCTCCTCCTCACCCTCCTCACTCACTGTGCAGGGTCCTGGGCCCAGTCTGTG8470.10618293049844549No Hit
CTGTAGGGGCCACTGTCTTCTCCACGGTGCTCCCTTCATGCGTGACCTGG8440.10580683983552303No Hit
GGCCCAGTCTGTGCTGACTCAGCCACCCTCAGCGTCTGGGACCCCCGGGC8290.10392638652091063No Hit
GTCTGGCACCTCAGCCTCCCTGGCCATCAGTGGGCTCCGGTCCGAGGATG8220.1030488416407582No Hit
GTCCGAGGATGAGGCGGATTATTATTGTGCAGCGTGGGATGACAGTGGTC8150.10217129676060575No Hit
GAGCGTCACCATCTCTTGTTTTGGAAACAGCTCCAACATCGGGAGGAACT8050.10091766121753083No Hit

[OK]Adapter Content

Adapter graph

[FAIL]Kmer Content

Kmer graph

SequenceCountPValueObs/Exp MaxMax Obs/Exp Position
CGGTACC407.8029916E-752.18054245
TAGGCGG200.00750277251.49591436
GTAGTTT1152.3731991E-741.2990470
TTAACGG503.9855695E-641.1784635
GGTATCA7750.040.7856941
TAGGGTG350.001457036238.8069885
GTATCAA20300.038.676091
GTGGTAT2550.037.320241
TGGTATC2650.035.880412
TAACCGT601.1661774E-535.21448553
TAACGGC601.3880193E-534.3306136
TATCAAC23500.032.9467242
GTACCCT851.09281245E-732.84197647
GCTTAGG553.5544042E-430.898251
TCAACGC25050.030.4975914
ATCAACG25200.030.4507963
CGTGCAA703.1696913E-530.42517357
AACGCAG25450.030.4242236
GTATATA450.00497244130.2116261
CAACGCA25450.030.020145
Produced by FastQC (version 0.11.5)