FastQCFastQC Report
Wed 27 Apr 2022
EGAF00004684394

Summary

[OK]Basic Statistics

MeasureValue
FilenameEGAF00004684394
File typeConventional base calls
EncodingSanger / Illumina 1.9
Total Sequences1162145
Sequences flagged as poor quality0
Sequence length20-76
%GC53

[OK]Per base sequence quality

Per base quality graph

[OK]Per tile sequence quality

Per tile quality graph

[OK]Per sequence quality scores

Per Sequence quality graph

[FAIL]Per base sequence content

Per base sequence content

[FAIL]Per sequence GC content

Per sequence GC content graph

[OK]Per base N content

N content graph

[WARN]Sequence Length Distribution

Sequence length distribution

[FAIL]Sequence Duplication Levels

Duplication level graph

[WARN]Overrepresented sequences

SequenceCountPercentagePossible Source
CTCCTGGGAGTTACCCGATTGGAGGGCGTTATCCACCTTCCACTGTACTTTGGCCTCTCTGGGATAGAAGTTATT51630.4442647001880144No Hit
CTCTAACACTCTCCCCTGTTGAAGCTCTTTGTGACGGGCGAGCTCAGGCC49620.426969095938975No Hit
GAGTTACATTATCCCCCGGACGTTCGGCCAAGGGGCCAAGGTGGAAATCAAACGAACTGTGGCTGCACCAT42430.36510074044116697No Hit
GGCGTAGACTTTGTGTTTCTCGTAGTCTGCTTTGCTCAGCGTCAGGGTGC33890.2916159343283325No Hit
CTCCCAGGAGAGTGTCACAGAGCAGGACAGCAAGGACAGCACCTACAGCC32970.2836995383536478No Hit
GGCTGGAACTGAGGAGCAGGTGGGGGCACTTCTCCCTCTAACACTCTCCC31560.2715668010446201No Hit
GTCTACAACCTGAAGATTTTGCAACTTACTACTGTCAACAGAGTTACATTATCCCCCGGACGTTCGGCCAAGGGG29980.25797125143592237No Hit
CAATAGGGGTAGGTCCCCTGTGGAAAAAGGGTCAGAGGCCAAAGGATGGGAGGGGGTCAGGCTGGAACTGAGGAG29470.25358281453691234No Hit
GTCTCAGTCAGGACACAGCATGGACATGAGGGTCCCCGCTCAGCTCCTGG28310.24360127178622287No Hit
CAACAGAGGCAGTTCCAGATTTCAACTGCTCATCAGATGGCGGGAAGATG27930.24033145605754877No Hit
GTCTACGCCTGCGAAGTCACCCATCAGGGCCTGAGCTCGCCCGTCACAAA27490.23654535363487345No Hit
GGATGGGAGGGGGTCAGGCTGGAACTGAGGAGCAGGTGGGGGCACTTCTCCCTCTAACACTCTCCCCTGTTGAAG26950.23189877338886283No Hit
CTTATACACATCTCCGAGCCCACGAGACATGCGCAGATCTCGTATGCCGT26060.2242405207611787No Hit
GTCCTGCTCTGTGACACTCTCCTGGGAGTTACCCGATTGGAGGGCGTTATCCACCTTCCACTGTACTTTGGCCT25820.22217537398517398No Hit
GATCTATTCTGCTTCCACTTTGCAAAGTGGGGTCCCATCAAGGTTCAGTGGTAGTGGATCTGGGACAGATTTCA25650.22071256168550396No Hit
ATGTAACTCTGTTGACAGTAGTAAGTTGCAAAATCTTCAGGTTGTAGACTGTTGATGGTGAGAGTGAAATCTGTC25610.22036837055616987No Hit
GCGTCAGGGTGCTGCTGAGGCTGTAGGTGCTGTCCTTGCTGTCCTGCTCT25560.21993813164450218No Hit
GTACAGTGGAAGGTGGATAACGCCCTCCAATCGGGTAACTCCCAGGAGAG24730.21279616571081925No Hit
GTAGGAGACAGAGTCACCATCACTTGCCGGACAAGTCAGACCATTAGTAACTATTTAAATTGGTATCAGCAGAAA24410.21004263667614625No Hit
GTCTCCATCCTCCCTGTCTGCATCTGTAGGAGACAGAGTCACCATCACTTGCCGGACAAGTCAGACCATTAGTAA22380.1925749368624397No Hit
GCATGGACATGAGGGTCCCCGCTCAGCTCCTGGGGCTCCTGCTACTCTGG21720.18689578322842673No Hit
CCACTGTACTTTGGCCTCTCTGGGATAGAAGTTATTCAGCAGGCACACAACAGAGGCAGTTCCAGATTTCAACTG21480.18483063645242204No Hit
CTCTGGGATAGAAGTTATTCAGCAGGCACACAACAGAGGCAGTTCCAGAT21100.1815608207237479No Hit
GTGCAGCCACAGTTCGTTTGATTTCCACCTTGGCCCCTTGGCCGAACGTC20670.17786076608340612No Hit
GTCTTCATCTTCCCGCCATCTGATGAGCAGTTGAAATCTGGAACTGCCTCTGTTGTGTGCCTGCTGAATAACTT20670.17786076608340612No Hit
GTGGCTGCACCATCTGTCTTCATCTTCCCGCCATCTGATGAGCAGTTGAAATCTGGAACTGCCTCTGTTGTGTGC20240.17416071144306433No Hit
ACATTATCCCCCGGACGTTCGGCCAAGGGGCCAAGGTGGAAATCAAACGA19810.17046065680272254No Hit
GAGCAGGACAGCAAGGACAGCACCTACAGCCTCAGCAGCACCCTGACGCT19750.16994437010872138No Hit
CTGCTACTCTGGCTCCGAGGTGCCAGATGTGACATCCAGATGACCCAGTC19500.16779317555038312No Hit
GGCTGTAGGTGCTGTCCTTGCTGTCCTGCTCTGTGACACTCTCCTGGGAGTTACCCGATTGGAGGGCGTTATCCA18330.15772558501736014No Hit
GTGGTAGTGGATCTGGGACAGATTTCACTCTCACCATCAACAGTCTACAACCTGAAGATTTTGCAACTTACTA18140.15609067715302308No Hit
CAGCATGGACATGAGGGTCCCCGCTCAGCTCCTGGGGCTCCTGCTACTCTGGCTCCGAGGTGCCAGATGTGACAT18060.15540229489435484No Hit
GGCCAAAGGATGGGAGGGGGTCAGGCTGGAACTGAGGAGCAGGTGGGGGCACTTCTCCCTCTAACACTCTCCC17840.15350924368301716No Hit
GATGAAGACAGATGGTGCAGCCACAGTTCGTTTGATTTCCACCTTGGCCC17360.14937895013100777No Hit
ACTTACTACTGTCAACAGAGTTACATTATCCCCCGGACGTTCGGCCAAGGGGCCAAGGTGGAAATCAAACGAA17160.14765799448433714No Hit
GGACACAGCATGGACATGAGGGTCCCCGCTCAGCTCCTGGGGCTCCTGCT17090.14705566000800244No Hit
GATTTCACTCTCACCATCAACAGTCTACAACCTGAAGATTTTGCAACTTACTACTGTCAACAGAGTTACATTATC17000.14628122996700066No Hit
GAATAGATCAGGAGGTTAGGGGCTTTCCCTGGTTTCTGCTGATACCAATT16610.14292536645599302No Hit
GGCCCTGATGGGTGACTTCGCAGGCGTAGACTTTGTGTTTCTCGTAGTCTGCTTTGCTCAGCGTCAGGGTGCTG16600.14283931867365948No Hit
ATCCCAGAGAGGCCAAAGTACAGTGGAAGGTGGATAACGCCCTCCAATCG16360.14077417189765476No Hit
GTTGTGTGCCTGCTGAATAACTTCTATCCCAGAGAGGCCAAAGTACAGTG15810.13604154386931064No Hit
CACCATCAACAGTCTACAACCTGAAGATTTTGCAACTTACTACTGTCAACAGAGTTACATTATCCCCCGGACGTT15420.13268568035830297No Hit
GTTGATGGTGAGAGTGAAATCTGTCCCAGATCCACTACCACTGAACCTTG14980.12889957793562765No Hit
GCTTTGCTCAGCGTCAGGGTGCTGCTGAGGCTGTAGGTGCTGTCCTTGCT14890.1281251478946259No Hit
GTCCTTGCTGTCCTGCTCTGTGACACTCTCCTGGGAGTTACCCGATTGGAGGGCGTTATCCACCTTCCACTGTA14850.12778095676529178No Hit
GTTCAGTGGTAGTGGATCTGGGACAGATTTCACTCTCACCATCAACAGTCTACAACCTGAAGATTTTGCAACTTA14600.12562976220695352No Hit
GTAGTAAGTTGCAAAATCTTCAGGTTGTAGACTGTTGATGGTGAGAGTGAAATCTGTCCCAGATCCACTACCA14290.12296228095461409No Hit
GGTGAAAGATGAGCTGGAGGACCGCAATAGGGGTAGGTCCCCTGTGGAAA14180.12201575534894528No Hit
GAGTGAAATCTGTCCCAGATCCACTACCACTGAACCTTGATGGGACCCCA14070.12106922974327644No Hit
GCTCCTGGGGCTCCTGCTACTCTGGCTCCGAGGTGCCAGATGTGACATCCAGATGACCCAGTCTCCATCCTCC14000.12046689526694174No Hit
GGATAATGTAACTCTGTTGACAGTAGTAAGTTGCAAAATCTTCAGGTTGTAGACTGTTGATGGTGAGAGTGAAAT13940.11995060857294054No Hit
GTACTTTGGCCTCTCTGGGATAGAAGTTATTCAGCAGGCACACAACAGAGGCAGTTCCAGATTTCAACTGCTCAT13620.1171970795382676No Hit
GTCTGCATCTGTAGGAGACAGAGTCACCATCACTTGCCGGACAAGTCAGA13580.11685288840893347No Hit
CCCCTGTTGAAGCTCTTTGTGACGGGCGAGCTCAGGCCCTGATGGGTGACTTCGCAGGCGTAGACTTTGTGTTT13510.11625055393259878No Hit
GTGGAAAAAGGGTCAGAGGCCAAAGGATGGGAGGGGGTCAGGCTGGAACTGAGGAGCAGGTGGGGGCACTTCTCC13460.11582031502093111No Hit
GTTGTAGACTGTTGATGGTGAGAGTGAAATCTGTCCCAGATCCACTACCA13370.11504588497992935No Hit
CCGCAATAGGGGTAGGTCCCCTGTGGAAAAAGGGTCAGAGGCCAAAGGAT13180.11341097711559228No Hit
GCACACAACAGAGGCAGTTCCAGATTTCAACTGCTCATCAGATGGCGGGA13160.11323888155092524No Hit
GTTGAAGCTCTTTGTGACGGGCGAGCTCAGGCCCTGATGGGTGACTTCGC13030.11212026038058935No Hit
GAGCAAAGCAGACTACGAGAAACACAAAGTCTACGCCTGCGAAGTCACCC12760.10979697025758405No Hit
GTGGGGTCCCATCAAGGTTCAGTGGTAGTGGATCTGGGACAGATTTCACT12750.1097109224752505No Hit
CCTCCAACATTAGCATAATTAAAGCCAAGGAGGAGGAGGGGGGTGAGGTG12740.10962487469291697No Hit
CCTCTGTTGTGTGCCTGCTGAATAACTTCTATCCCAGAGAGGCCAAAGTA12730.10953882691058345No Hit
GTGGAAGGTGGATAACGCCCTCCAATCGGGTAACTCCCAGGAGAGTGTCACAGAGCAGGACAGCAAGGACAGCAC12670.10902254021658228No Hit
GGGTAGGTCCCCTGTGGAAAAAGGGTCAGAGGCCAAAGGATGGGAGGGGG12600.10842020574024756No Hit
GCCCCTAACCTCCTGATCTATTCTGCTTCCACTTTGCAAAGTGGGGTCCCATCAAGGTTCAGTGGTAGTGGAT12520.10773182348157932No Hit
GTCAGGACACAGCATGGACATGAGGGTCCCCGCTCAGCTCCTGGGGCTCC12400.10669925009357695No Hit
ACCTTCCACTGTACTTTGGCCTCTCTGGGATAGAAGTTATTCAGCAGGCA12330.10609691561724226No Hit
GAGGAGGAGGGGGGTGAGGTGAAAGATGAGCTGGAGGACCGCAATAGGGG12200.10497829444690637No Hit
GAACTGAGGAGCAGGTGGGGGCACTTCTCCCTCTAACACTCTCCCCTGTT12130.10437595997057166No Hit
GCTCAGTAGCAGGTGCCGTCCACCTCCGCCATGACAACAGACACATTGAC12080.10394572105890401No Hit
CTTTGTGACGGGCGAGCTCAGGCCCTGATGGGTGACTTCGCAGGCGTAGA12030.10351548214723635No Hit
GTTGAAATCTGGAACTGCCTCTGTTGTGTGCCTGCTGAATAACTTCTATC11830.10179452650056578No Hit
CTTCTATCCCAGAGAGGCCAAAGTACAGTGGAAGGTGGATAACGCCCTCCAATCGGGTAACTCCCAGGAGAGTGT11820.10170847871823223No Hit
AGTCAGACCATTAGTAACTATTTAAATTGGTATCAGCAGAAACCAGGGAAAGCCCCTAACCTCCTGATCTATT11810.1016224309358987No Hit
GTATTATAGTGGGAGCAAAATGTACAATCCGTCCCTCGAGAGCCGAGTCA11790.10145033537123166No Hit
GGTTAGGGGCTTTCCCTGGTTTCTGCTGATACCAATTTAAATAGTTACTA11770.10127823980656459No Hit
GTGTCACAGAGCAGGACAGCAAGGACAGCACCTACAGCCTCAGCAGCACC11750.10110614424189754No Hit

[OK]Adapter Content

Adapter graph

Produced by FastQC (version 0.11.9)