FastQCFastQC Report
Sat 23 Apr 2022
EGAF00004684396

Summary

[OK]Basic Statistics

MeasureValue
FilenameEGAF00004684396
File typeConventional base calls
EncodingSanger / Illumina 1.9
Total Sequences1188959
Sequences flagged as poor quality0
Sequence length20-76
%GC55

[OK]Per base sequence quality

Per base quality graph

[OK]Per tile sequence quality

Per base quality graph

[OK]Per sequence quality scores

Per Sequence quality graph

[FAIL]Per base sequence content

Per base sequence content

[WARN]Per sequence GC content

Per sequence GC content graph

[OK]Per base N content

N content graph

[WARN]Sequence Length Distribution

Sequence length distribution

[FAIL]Sequence Duplication Levels

Duplication level graph

[WARN]Overrepresented sequences

SequenceCountPercentagePossible Source
CTCCTGGGAGTTACCCGATTGGAGGGCGTTATCCACCTTCCACTGTACTTTGGCCTCTCTGGGATAGAAGTTATT44960.37814592429175437No Hit
CTCTAACACTCTCCCCTGTTGAAGCTCTTTGTGACGGGCGAGCTCAGGCC43570.36645502494198706No Hit
GGCTGGAACTGAGGAGCAGGTGGGGGCACTTCTCCCTCTAACACTCTCCC28130.2365935242510465No Hit
GTAATGGATACAACTATTTGGATTGGTACCTGCAGAAGCCAGGGCAGTCTCCACAGCTCCTGATCTATTTGGGTT27570.2318835216353129No Hit
CTCCTGTACAGCCTCTGGATTCACATTTGGTGATTATGCTCTGACCTGGATCCGCCAGGCTCCAGGGAAGGGG27260.2292761987587461No Hit
CTCCCAGGAGAGTGTCACAGAGCAGGACAGCAAGGACAGCACCTACAGCC27220.2289397700004794No Hit
CCCCTGGAGAGCCGGCCTCCATCTCCTGCAGGTCTAGTCAGAGCCTCCTG27060.22759405496741267No Hit
CAACAGAGGCAGTTCCAGATTTCAACTGCTCATCAGATGGCGGGAAGATG26610.22380923143691245No Hit
CTACAAACTCCTTGGACGTTCGGCCAAGGGACCAAGGTGGAAATCAAACG25810.21708065627157874No Hit
GTAATATAGGGCTGTGTCCTCGGTTTTCAGGCTGTTCATTTGCAGATAGG24280.20421225626787803No Hit
GTAATAAACCCCAACATCCTCAGCCTCCGCTCTGCTGATTTTCAGTGTAAAATCTGTGCCTGATCCACTGCCA24240.20387582750961133No Hit
CAATAGGGGTAGGTCCCCTGTGGAAAAAGGGTCAGAGGCCAAAGGATGGGAGGGGGTCAGGCTGGAACTGAGGAG24210.20362350594091133No Hit
GATCAGGACTCCTCAGTTCACCTTCTCACAATGAGGCTCCCTGCTCAGCTCCTGGGGCTGCTAATGCTCTGGGT23860.20067975430607785No Hit
GGCGTAGAGTTTGTGTTTCTCGTAGTCTGCTTTGCTCAGCGTCAGGGTGC23560.1981565386190777No Hit
GGATGGGAGGGGGTCAGGCTGGAACTGAGGAGCAGGTGGGGGCACTTCTCCCTCTAACACTCTCCCCTGTTGAAG23480.19748368110254433No Hit
GTCCTGCTCTGTGACACTCTCCTGGGAGTTACCCGATTGGAGGGCGTTATCCACCTTCCACTGTACTTTGGCCT23470.19739957391297766No Hit
GTGCAGTGACAGTCGGGGGTGGCAGCATGAGGGAGATGGGGTAGGTGGAG23120.19445582227814417No Hit
GGTCTAGTCAGAGCCTCCTGCATGGTAATGGATACAACTATTTGGATTGGTACCTGCAGAAGCCAGGGCAGTCTC22640.19041867717894395No Hit
GTCCCTGACAGGCTCAGTGGCAGTGGATCAGGCACAGATTTTACACTGAA22580.1899140340415439No Hit
GCGTCAGGGTGCTGCTGAGGCTGTAGGTGCTGTCCTTGCTGTCCTGCTCT21780.18318545887621018No Hit
GAGTTTGTAGAGCTTGCATGCAGTAATAAACCCCAACATCCTCAGCCTCCGCTCTGCTGATTTTCAGTGTAAAAT21630.18192385103271014No Hit
GTACAGTGGAAGGTGGATAACGCCCTCCAATCGGGTAACTCCCAGGAGAG21580.18150331508487677No Hit
GGACTCCTCAGTTCACCTTCTCACAATGAGGCTCCCTGCTCAGCTCCTGG21460.1804940288100767No Hit
CTCCTTGGACGTTCGGCCAAGGGACCAAGGTGGAAATCAAACGAACTGTGGCTGCACCATCTGTCTTCATCTTCC21040.1769615268482765No Hit
GTGCAGCCACAGTTCGTTTGATTTCCACCTTGGTCCCTTGGCCGAACGTC20850.17536349024650977No Hit
CTCTGGGATAGAAGTTATTCAGCAGGCACACAACAGAGGCAGTTCCAGAT20110.16913955821857607No Hit
CCACTGTACTTTGGCCTCTCTGGGATAGAAGTTATTCAGCAGGCACACAACAGAGGCAGTTCCAGATTTCAACTG19540.1643454484132758No Hit
GCCCTATATTACTGTGCTAGAGAGAGGTCATCCACAGTACTTTGGTTCGG19530.16426134122370914No Hit
GTCAGGGACCCCGGAGGCCCGATTAGAACCCAAATAGATCAGGAGCTGTGGAGACTGCCCTGGCTTCTGCAGGTA19100.16064473207234228No Hit
GTCTTCATCTTCCCGCCATCTGATGAGCAGTTGAAATCTGGAACTGCCTCTGTTGTGTGCCTGCTGAATAACTT18800.15812151638534214No Hit
GTGGCTGCACCATCTGTCTTCATCTTCCCGCCATCTGATGAGCAGTTGAAATCTGGAACTGCCTCTGTTGTGTGC18580.15627115821487536No Hit
GTCACTGCACCGACCGGCCCTCGAGGACCTGCTCTTAGGTTCAGAAGCGAACCTCACGTGCACACTGACCGGC18410.15484133599224195No Hit
GATTTTACACTGAAAATCAGCAGAGCGGAGGCTGAGGATGTTGGGGTTTATTACTGCATGCAAGCTCTACAAACT18150.15265454906350848No Hit
GATGAAGACAGATGGTGCAGCCACAGTTCGTTTGATTTCCACCTTGGTCC17970.1511406196513084No Hit
GTCACATCCTGGCTGGGATTCGTGTAGTGCTTCACGTGGCATGTCACGGA17860.15021544056607503No Hit
GTATGCTGGTCACAGCGAAGGTGGTGGTGCCCTGGCTGGGCTCCTGCCGG17710.14895383272257495No Hit
ATATAGGGCTGTGTCCTCGGTTTTCAGGCTGTTCATTTGCAGATAGGCGA17570.14777633206864155No Hit
GTCTCCACTCTCCCTGCCCGTCACCCCTGGAGAGCCGGCCTCCATCTCCT17430.14659883141470817No Hit
GGCTGTAGGTGCTGTCCTTGCTGTCCTGCTCTGTGACACTCTCCTGGGAGTTACCCGATTGGAGGGCGTTATCCA17390.14626240265644147No Hit
GAGCAGGACAGCAAGGACAGCACCTACAGCCTCAGCAGCACCCTGACGCT16840.14163650723027454No Hit
CAATGAGGCTCCCTGCTCAGCTCCTGGGGCTGCTAATGCTCTGGGTCTCTGGATCCAGTGGGGATATTGTGATGA16240.13659007585627428No Hit
GCTCAGTAGCAGGTGCCGTCCACCTCCGCCATGACAACAGACACATTGAC16220.13642186147714092No Hit
GGCCAAAGGATGGGAGGGGGTCAGGCTGGAACTGAGGAGCAGGTGGGGGCACTTCTCCCTCTAACACTCTCCC16180.13608543271887424No Hit
GCTAATGCTCTGGGTCTCTGGATCCAGTGGGGATATTGTGATGACTCAGTCTCCACTCTCCCTGCCCGTCACCC16160.1359172183397409No Hit
ATATTACTGTGCTAGAGAGAGGTCATCCACAGTACTTTGGTTCGGGGAGTTATCTTCGGGCTACGGTATGGACGT15880.1335622170318741No Hit
ATCCCAGAGAGGCCAAAGTACAGTGGAAGGTGGATAACGCCCTCCAATCG15860.13339400265274076No Hit
GGCCCTGATGGGTGACTTCGCAGGCGTAGAGTTTGTGTTTCTCGTAGTCTGCTTTGCTCAGCGTCAGGGTGCTG15780.1327211451362074No Hit
CTATATTACTGTGCTAGAGAGAGGTCATCCACAGTACTTTGGTTCGGGGA15640.13154364448227399No Hit
CTCTACGCCTGCGAAGTCACCCATCAGGGCCTGAGCTCGCCCGTCACAAA15600.13120721572400731No Hit
AGCCAGGGCAGTCTCCACAGCTCCTGATCTATTTGGGTTCTAATCGGGCC15560.13087078696574062No Hit
GCACTGAACACAGACCACCAACCATGGAGTTTGGGCTTAGCTGGGTTTTC15550.13078667977617395No Hit
CCTCAGTTCACCTTCTCACAATGAGGCTCCCTGCTCAGCTCCTGGGGCTG15450.12994560788050724No Hit
GTGTACAGGTCCCCGGAGGCATCCTGGCTGGGTGGGAAGTTTCTGGCGGT15440.12986150069094057No Hit
GTGTGACCTGGAGCGAAAGCGGACAGGGCGTGACCGCCAGAAACTTCCCACCCAGCCAGGATGCCTCCGGGGAC15410.12960917912224054No Hit
TCCAAGGAGTTTGTAGAGCTTGCATGCAGTAATAAACCCCAACATCCTCA15320.1288522144161405No Hit
GCTCCTGGGGCTGCTAATGCTCTGGGTCTCTGGATCCAGTGGGGATATTGTGATGACTCAGTCTCCACTCTCC15280.1285157856578738No Hit
GTTCAAGGACCACCTGAGCGTGACCTCTGTGGCTGCTACAGCGTGTCCAG15180.1276747137622071No Hit
AGGTCACACTGAGTGGCTCCTGGGGGAAGAAGCCCTGGACCAGGCAGGCG14890.12523560526477365No Hit
GTGTAAAATCTGTGCCTGATCCACTGCCACTGAGCCTGTCAGGGACCCCG14740.12397399742127357No Hit
GTTGTGTGCCTGCTGAATAACTTCTATCCCAGAGAGGCCAAAGTACAGTG14590.1227123895777735No Hit
GTCCTTGCTGTCCTGCTCTGTGACACTCTCCTGGGAGTTACCCGATTGGAGGGCGTTATCCACCTTCCACTGTA14300.12027328108034004No Hit
GTTTATTACTGCATGCAAGCTCTACAAACTCCTTGGACGTTCGGCCAAGGGACCAAGGTGGAAATCAAACGAA14040.11808649415160656No Hit
GTCTTGGACTCGGGGTAGGCAGCAGTGCAAGTGAAGGTCTTCCCATGGTT13890.11682488630810649No Hit
GCTTTGCTCAGCGTCAGGGTGCTGCTGAGGCTGTAGGTGCTGTCCTTGCT13780.11589970722287311No Hit
GAGTTTATTCAGGGGTGGGGACAGGCGGGCGGCTCAGTAGCAGGTGCCGT13770.11581560003330646No Hit
TTATTACTGCATGCAAGCTCTACAAACTCCTTGGACGTTCGGCCAAGGGA13670.11497452813763974No Hit
GCCCCAAGGTCTTCCCGCTGAGCCTCTGCAGCACCCAGCCAGATGGGAAC13530.11379702748370633No Hit
ATCCATTACCATGCAGGAGGCTCTGACTAGACCTGCAGGAGATGGAGGCC13500.11354470591500633No Hit
GTATGGACGTCTGGGGCCACGGGACCACGGTCACCGTCTCCTCAGCATCCCCGACCAGCCCCAAGGTCTTCCCG13270.11161024055497289No Hit
CAGTAATAAACCCCAACATCCTCAGCCTCCGCTCTGCTGATTTTCAGTGTAAAATCTGTGCCTGATCCACTGCCA13260.1115261333654062No Hit
GTCCAGGGCTTCTTCCCCCAGGAGCCACTCAGTGTGACCTGGAGCGAAAGCGGACAGGGCGTGACCGCCAGAAA13250.11144202617583954No Hit
GTGTGCACGTGAGGTTCGCTTCTGAACCTAAGAGCAGGTCCTCGAGGGCC13220.11118970460713953No Hit
CTTATACACATCTCCGAGCCCACGAGACACTCGCTAATCTCGTATGCCGT13140.11051684709060615No Hit
GTACTTTGGCCTCTCTGGGATAGAAGTTATTCAGCAGGCACACAACAGAGGCAGTTCCAGATTTCAACTGCTCAT13120.11034863271147281No Hit
GTTGAAGCTCTTTGTGACGGGCGAGCTCAGGCCCTGATGGGTGACTTCGC13000.10933934643667276No Hit
GTGCCTAGCCGGCAAGTCCGTGACATGCCACGTGAAGCACTACACGAATCCCAGCCAGGATGTGACTGTGCCCTG13000.10933934643667276No Hit
GCACACAACAGAGGCAGTTCCAGATTTCAACTGCTCATCAGATGGCGGGA12900.10849827454100605No Hit
GGATACAACTATTTGGATTGGTACCTGCAGAAGCCAGGGCAGTCTCCACAGCTCCTGATCTATTTGGGTTCTAAT12820.10782541702447268No Hit
CGTGCACACTGACCGGCCTGAGAGATGCCTCAGGTGTCACCTTCACCTGGACGCCCTCAAGTGGGAAGAGCG12730.10706845231837263No Hit
CTCCAGGGGTGACGGGCAGGGAGAGTGGAGACTGAGTCATCACAATATCC12700.10681613074967262No Hit
ACCTTCCACTGTACTTTGGCCTCTCTGGGATAGAAGTTATTCAGCAGGCA12360.10395648630440579No Hit
CAGTAATATAGGGCTGTGTCCTCGGTTTTCAGGCTGTTCATTTGCAGATA12310.10353595035657243No Hit
GCTCAGCTCCTGGGGCTGCTAATGCTCTGGGTCTCTGGATCCAGTGGGGATATTGTGATGACTCAGTCTCCACT12150.1021902353235057No Hit
CAGTATGCTGGTCACAGCGAAGGTGGTGGTGCCCTGGCTGGGCTCCTGCC12110.101853806565239No Hit
CCCCTGTTGAAGCTCTTTGTGACGGGCGAGCTCAGGCCCTGATGGGTGACTTCGCAGGCGTAGAGTTTGTGTTT12070.10151737780697231No Hit
GGATGTGACTGTGCCCTGCCCAGTTCCCTCAACTCCACCTACCCCATCTCCCTCAACTCCACCTACCCCATCTCC11990.10084452029043894No Hit
GTGGAAAAAGGGTCAGAGGCCAAAGGATGGGAGGGGGTCAGGCTGGAACTGAGGAGCAGGTGGGGGCACTTCTCC11930.10033987715303892No Hit
CTCCCTGCCCGTCACCCCTGGAGAGCCGGCCTCCATCTCCTGCAGGTCTA11900.10008755558433892No Hit

[OK]Adapter Content

Adapter graph

[WARN]Kmer Content

Kmer graph

SequenceCountPValueObs/Exp MaxMax Obs/Exp Position
TACGTAT300.003273947263.49729570
GTACGTA250.007797234750.99770469
CGTATGC2500.047.04043641
TCGTATG2600.045.17524740
GGTATCA7850.045.155311
CGCTAAT2450.044.71864332
GCTAATC2250.044.15846633
TCGCTAA2650.043.88496831
CACTCGC2650.043.80728528
ACACTCG2650.043.78329527
GTGGTAT1900.043.052941
GTATGCC2850.042.5334742
ACTCGCT2750.042.23565729
GCCGTCT2750.041.78996746
CTAATCT2400.041.4423834
ACGAGAC2800.041.37675522
CGAGACA2900.041.12843323
ATCTCGT2450.040.71901737
ATCTCCG2850.040.6457210
TATACAC2700.040.3766673
Produced by FastQC (version 0.11.5)