FastQCFastQC Report
Sat 23 Apr 2022
EGAF00004684397

Summary

[OK]Basic Statistics

MeasureValue
FilenameEGAF00004684397
File typeConventional base calls
EncodingSanger / Illumina 1.9
Total Sequences1188959
Sequences flagged as poor quality0
Sequence length20-76
%GC55

[OK]Per base sequence quality

Per base quality graph

[OK]Per tile sequence quality

Per base quality graph

[OK]Per sequence quality scores

Per Sequence quality graph

[FAIL]Per base sequence content

Per base sequence content

[WARN]Per sequence GC content

Per sequence GC content graph

[OK]Per base N content

N content graph

[WARN]Sequence Length Distribution

Sequence length distribution

[FAIL]Sequence Duplication Levels

Duplication level graph

[WARN]Overrepresented sequences

SequenceCountPercentagePossible Source
CTCCTGGGAGTTACCCGATTGGAGGGCGTTATCCACCTTCCACTGTACTTTGGCCTCTCTGGGATAGAAGTTATT45010.37856646023958773No Hit
CTCTAACACTCTCCCCTGTTGAAGCTCTTTGTGACGGGCGAGCTCAGGCC36970.310944279827984No Hit
CTCCCAGGAGAGTGTCACAGAGCAGGACAGCAAGGACAGCACCTACAGCC29780.2504712105295473No Hit
GGGGGGGGGGGGGGGGGGGGGGGGGGGGGGGGGGGGGGGGGGGGGGGGGG27940.23499548764927977No Hit
CTCCTGTACAGCCTCTGGATTCACATTTGGTGATTATGCTCTGACCTGGATCCGCCAGGCTCCAGGGAAGGGG27770.23356566542664634No Hit
CCCCTGGAGAGCCGGCCTCCATCTCCTGCAGGTCTAGTCAGAGCCTCCTG26770.2251549464699792No Hit
GGCTGGAACTGAGGAGCAGGTGGGGGCACTTCTCCCTCTAACACTCTCCC25740.21649190594461207No Hit
GTAATGGATACAACTATTTGGATTGGTACCTGCAGAAGCCAGGGCAGTCTCCACAGCTCCTGATCTATTTGGGTT24990.21018386672711167No Hit
CTACAAACTCCTTGGACGTTCGGCCAAGGGACCAAGGTGGAAATCAAACG24720.20791297260881159No Hit
CAACAGAGGCAGTTCCAGATTTCAACTGCTCATCAGATGGCGGGAAGATG24450.20564207849051147No Hit
GTAATAAACCCCAACATCCTCAGCCTCCGCTCTGCTGATTTTCAGTGTAAAATCTGTGCCTGATCCACTGCCA23590.1984088601877777No Hit
GTCCTGCTCTGTGACACTCTCCTGGGAGTTACCCGATTGGAGGGCGTTATCCACCTTCCACTGTACTTTGGCCT23530.19790421705037767No Hit
GATCAGGACTCCTCAGTTCACCTTCTCACAATGAGGCTCCCTGCTCAGCTCCTGGGGCTGCTAATGCTCTGGGT22850.19218492815984403No Hit
GTAATATAGGGCTGTGTCCTCGGTTTTCAGGCTGTTCATTTGCAGATAGG22630.19033456998937726No Hit
GAGTTTGTAGAGCTTGCATGCAGTAATAAACCCCAACATCCTCAGCCTCCGCTCTGCTGATTTTCAGTGTAAAAT22400.1884001046293438No Hit
GGCGTAGAGTTTGTGTTTCTCGTAGTCTGCTTTGCTCAGCGTCAGGGTGC21820.18352188763447688No Hit
GGTCTAGTCAGAGCCTCCTGCATGGTAATGGATACAACTATTTGGATTGGTACCTGCAGAAGCCAGGGCAGTCTC21740.1828490301179435No Hit
GCGTCAGGGTGCTGCTGAGGCTGTAGGTGCTGTCCTTGCTGTCCTGCTCT21080.1772979556065432No Hit
GTACAGTGGAAGGTGGATAACGCCCTCCAATCGGGTAACTCCCAGGAGAG20480.17225152423254292No Hit
GTGCAGCCACAGTTCGTTTGATTTCCACCTTGGTCCCTTGGCCGAACGTC20030.16846670070204273No Hit
GGATGGGAGGGGGTCAGGCTGGAACTGAGGAGCAGGTGGGGGCACTTCTCCCTCTAACACTCTCCCCTGTTGAAG19920.16754152161680932No Hit
GTCCCTGACAGGCTCAGTGGCAGTGGATCAGGCACAGATTTTACACTGAA19900.167373307237676No Hit
CTCCTTGGACGTTCGGCCAAGGGACCAAGGTGGAAATCAAACGAACTGTGGCTGCACCATCTGTCTTCATCTTCC19750.16611169939417592No Hit
GAGCAGGACAGCAAGGACAGCACCTACAGCCTCAGCAGCACCCTGACGCT19710.16577527063590922No Hit
CTCTGGGATAGAAGTTATTCAGCAGGCACACAACAGAGGCAGTTCCAGAT19520.16417723403414247No Hit
GCCCTATATTACTGTGCTAGAGAGAGGTCATCCACAGTACTTTGGTTCGG19370.1629156261906424No Hit
GGACTCCTCAGTTCACCTTCTCACAATGAGGCTCCCTGCTCAGCTCCTGG19330.16257919743237573No Hit
CAATAGGGGTAGGTCCCCTGTGGAAAAAGGGTCAGAGGCCAAAGGATGGGAGGGGGTCAGGCTGGAACTGAGGAG19280.16215866148454236No Hit
GTCTTCATCTTCCCGCCATCTGATGAGCAGTTGAAATCTGGAACTGCCTCTGTTGTGTGCCTGCTGAATAACTT19150.16106526802017565No Hit
GTGGCTGCACCATCTGTCTTCATCTTCCCGCCATCTGATGAGCAGTTGAAATCTGGAACTGCCTCTGTTGTGTGC19150.16106526802017565No Hit
CCACTGTACTTTGGCCTCTCTGGGATAGAAGTTATTCAGCAGGCACACAACAGAGGCAGTTCCAGATTTCAACTG18570.1561870510253087No Hit
GTGCAGTGACAGTCGGGGGTGGCAGCATGAGGGAGATGGGGTAGGTGGAG18420.15492544318180862No Hit
GGCTGTAGGTGCTGTCCTTGCTGTCCTGCTCTGTGACACTCTCCTGGGAGTTACCCGATTGGAGGGCGTTATCCA18320.1540843712861419No Hit
GATGAAGACAGATGGTGCAGCCACAGTTCGTTTGATTTCCACCTTGGTCC17480.14701936736254154No Hit
GTCAGGGACCCCGGAGGCCCGATTAGAACCCAAATAGATCAGGAGCTGTGGAGACTGCCCTGGCTTCTGCAGGTA17400.14634650984600814No Hit
GATTTTACACTGAAAATCAGCAGAGCGGAGGCTGAGGATGTTGGGGTTTATTACTGCATGCAAGCTCTACAAACT17230.14491668762337473No Hit
GTCACTGCACCGACCGGCCCTCGAGGACCTGCTCTTAGGTTCAGAAGCGAACCTCACGTGCACACTGACCGGC17030.1432345438320413No Hit
GTCTCCACTCTCCCTGCCCGTCACCCCTGGAGAGCCGGCCTCCATCTCCT16970.14272990069464128No Hit
GTTCAAGGACCACCTGAGCGTGACCTCTGTGGCTGCTACAGCGTGTCCAG16260.13675829023540761No Hit
GTGTGACCTGGAGCGAAAGCGGACAGGGCGTGACCGCCAGAAACTTCCCACCCAGCCAGGATGCCTCCGGGGAC16180.13608543271887424No Hit
CCCTGACGCTGAGCAAAGCAGACTACGAGAAACACAAACTCTACGCCTGCGAAGTCACCCATCAGGGCCTGAGCT16110.13549668239190754No Hit
ATATTACTGTGCTAGAGAGAGGTCATCCACAGTACTTTGGTTCGGGGAGTTATCTTCGGGCTACGGTATGGACGT15950.1341509673588408No Hit
TCCAAGGAGTTTGTAGAGCTTGCATGCAGTAATAAACCCCAACATCCTCA15790.13280525232577406No Hit
GTGTACAGGTCCCCGGAGGCATCCTGGCTGGGTGGGAAGTTTCTGGCGGT15570.13095489415530728No Hit
ATATAGGGCTGTGTCCTCGGTTTTCAGGCTGTTCATTTGCAGATAGGCGA15570.13095489415530728No Hit
GTCACATCCTGGCTGGGATTCGTGTAGTGCTTCACGTGGCATGTCACGGA15570.13095489415530728No Hit
ATCCCAGAGAGGCCAAAGTACAGTGGAAGGTGGATAACGCCCTCCAATCG15350.1291045359848405No Hit
GCACTGAACACAGACCACCAACCATGGAGTTTGGGCTTAGCTGGGTTTTC15180.1276747137622071No Hit
GCTCCTGGGGCTGCTAATGCTCTGGGTCTCTGGATCCAGTGGGGATATTGTGATGACTCAGTCTCCACTCTCC15000.12616078435000702No Hit
CAATGAGGCTCCCTGCTCAGCTCCTGGGGCTGCTAATGCTCTGGGTCTCTGGATCCAGTGGGGATATTGTGATGA14810.12456274774824026No Hit
CTCTACGCCTGCGAAGTCACCCATCAGGGCCTGAGCTCGCCCGTCACAAA14580.12262828238820682No Hit
AGCCAGGGCAGTCTCCACAGCTCCTGATCTATTTGGGTTCTAATCGGGCC14560.12246006800907347No Hit
CTCCCTGCCCGTCACCCCTGGAGAGCCGGCCTCCATCTCCTGCAGGTCTA14410.12119846016557341No Hit
GTCCTTGCTGTCCTGCTCTGTGACACTCTCCTGGGAGTTACCCGATTGGAGGGCGTTATCCACCTTCCACTGTA14390.12103024578644009No Hit
GTTGTGTGCCTGCTGAATAACTTCTATCCCAGAGAGGCCAAAGTACAGTG14360.12077792421774006No Hit
CTATATTACTGTGCTAGAGAGAGGTCATCCACAGTACTTTGGTTCGGGGA14250.11985274513250667No Hit
GCTAATGCTCTGGGTCTCTGGATCCAGTGGGGATATTGTGATGACTCAGTCTCCACTCTCCCTGCCCGTCACCC14230.11968453075337333No Hit
GTTTATTACTGCATGCAAGCTCTACAAACTCCTTGGACGTTCGGCCAAGGGACCAAGGTGGAAATCAAACGAA14120.11875935166813995No Hit
CCTCAGTTCACCTTCTCACAATGAGGCTCCCTGCTCAGCTCCTGGGGCTG13860.11657256473940648No Hit
GGCCAAAGGATGGGAGGGGGTCAGGCTGGAACTGAGGAGCAGGTGGGGGCACTTCTCCCTCTAACACTCTCCC13810.11615202879157313No Hit
GTCCAGGGCTTCTTCCCCCAGGAGCCACTCAGTGTGACCTGGAGCGAAAGCGGACAGGGCGTGACCGCCAGAAA13800.11606792160200646No Hit
GGCCCTGATGGGTGACTTCGCAGGCGTAGAGTTTGTGTTTCTCGTAGTCTGCTTTGCTCAGCGTCAGGGTGCTG13520.11371292029413967No Hit
CAGTAATAAACCCCAACATCCTCAGCCTCCGCTCTGCTGATTTTCAGTGTAAAATCTGTGCCTGATCCACTGCCA13510.11362881310457298No Hit
GTGTAAAATCTGTGCCTGATCCACTGCCACTGAGCCTGTCAGGGACCCCG13400.11270363401933961No Hit
GTACTTTGGCCTCTCTGGGATAGAAGTTATTCAGCAGGCACACAACAGAGGCAGTTCCAGATTTCAACTGCTCAT13350.11228309807150626No Hit
GTATGGACGTCTGGGGCCACGGGACCACGGTCACCGTCTCCTCAGCATCCCCGACCAGCCCCAAGGTCTTCCCG13230.1112738117967062No Hit
CCTACAGCCTCAGCAGCACCCTGACGCTGAGCAAAGCAGACTACGAGAAACACAAACTCTACGCCTGCGAAGTCA13100.11018041833233948No Hit
TTATTACTGCATGCAAGCTCTACAAACTCCTTGGACGTTCGGCCAAGGGA13090.11009631114277278No Hit
GTCCAAGACCCCGCTAACCGCCACCCTCTCAAAATCCGGAAACACATTCC13020.10950756081580611No Hit
AGGTCACACTGAGTGGCTCCTGGGGGAAGAAGCCCTGGACCAGGCAGGCG12880.1083300601618727No Hit
GGATACAACTATTTGGATTGGTACCTGCAGAAGCCAGGGCAGTCTCCACAGCTCCTGATCTATTTGGGTTCTAAT12790.10757309545577265No Hit
ACCTTCCACTGTACTTTGGCCTCTCTGGGATAGAAGTTATTCAGCAGGCA12750.10723666669750598No Hit
GCTCAGTAGCAGGTGCCGTCCACCTCCGCCATGACAACAGACACATTGAC12590.10589095166443922No Hit
CGTGCACACTGACCGGCCTGAGAGATGCCTCAGGTGTCACCTTCACCTGGACGCCCTCAAGTGGGAAGAGCG12450.10471345101050583No Hit
CCTCTGTTGTGTGCCTGCTGAATAACTTCTATCCCAGAGAGGCCAAAGTA12430.1045452366313725No Hit
GCCCCAAGGTCTTCCCGCTGAGCCTCTGCAGCACCCAGCCAGATGGGAAC12290.10336773597743908No Hit
GTGCCTAGCCGGCAAGTCCGTGACATGCCACGTGAAGCACTACACGAATCCCAGCCAGGATGTGACTGTGCCCTG12280.10328362878787242No Hit
GTCTTGGACTCGGGGTAGGCAGCAGTGCAAGTGAAGGTCTTCCCATGGTT12200.10261077127133905No Hit
GCTTTGCTCAGCGTCAGGGTGCTGCTGAGGCTGTAGGTGCTGTCCTTGCT12170.10235844970263902No Hit
CTCCAGGGGTGACGGGCAGGGAGAGTGGAGACTGAGTCATCACAATATCC12110.101853806565239No Hit

[OK]Adapter Content

Adapter graph

[WARN]Kmer Content

Kmer graph

SequenceCountPValueObs/Exp MaxMax Obs/Exp Position
GCGTACC200.007182404452.06943
GTGGTAT2300.048.8404851
TGGTATC2700.041.559362
GGTATCA7450.039.2948841
GTATCAA19050.038.2395361
AACGCAG23450.031.610576
ATCAACG23350.031.3051243
TATCAAC23400.031.2421762
CAACGCA23850.031.0791055
TCAACGC23650.030.909324
ACGCAGA24300.030.364927
TACGAAT450.004962669730.224992
CGCAGAG24950.029.5738498
TTAACGG703.9743238E-529.4352235
GCAGAGT25900.028.4890949
TCTTGAG10750.028.07993570
CGGTACC755.8233018E-527.83107845
TGGCGTA500.0080001527.41000433
CGCGACG500.00827681127.21966419
GAGTAAT659.36243E-426.1848741
Produced by FastQC (version 0.11.5)