FastQCFastQC Report
Wed 27 Apr 2022
EGAF00004684398

Summary

[OK]Basic Statistics

MeasureValue
FilenameEGAF00004684398
File typeConventional base calls
EncodingSanger / Illumina 1.9
Total Sequences994863
Sequences flagged as poor quality0
Sequence length20-76
%GC53

[OK]Per base sequence quality

Per base quality graph

[OK]Per tile sequence quality

Per tile quality graph

[OK]Per sequence quality scores

Per Sequence quality graph

[FAIL]Per base sequence content

Per base sequence content

[FAIL]Per sequence GC content

Per sequence GC content graph

[OK]Per base N content

N content graph

[WARN]Sequence Length Distribution

Sequence length distribution

[FAIL]Sequence Duplication Levels

Duplication level graph

[WARN]Overrepresented sequences

SequenceCountPercentagePossible Source
CTCTAACACTCTCCCCTGTTGAAGCTCTTTGTGACGGGCGAGCTCAGGCC36040.36226093442011614No Hit
CTCCTGGGAGTTACCCGATTGGAGGGCGTTATCCACCTTCCACTGTACTTTGGCCTCTCTGGGATAGAAGTTATT33780.33954423875448175No Hit
CCCCTGGAGAGTCGGCCTCCATCTCCTGCAGGTCTAGTGAGAACCTCTGG27040.2717962171675899No Hit
CTCCCAGGAGAGTGTCACAGAGCAGGACAGCAAGGACAGCACCTACAGCC25210.2534017246595762No Hit
GGCTGGAACTGAGGAGCAGGTGGGGGCACTTCTCCCTCTAACACTCTCCC25180.2531001756020678No Hit
CTCTAGAACCTCCGTACACTTTTGGCCAGGGGACCAAGCTGGAGATCAAA24480.24606403092687135No Hit
TCTCTACACTGGTACCTACAGCAGCCAGGGCAGCCTCCACGACTCCTGAT21510.21621067423353768No Hit
CAATAGGGGTAGGTCCCCTGTGGAAAAAGGGTCAGAGGCCAAAGGATGGGAGGGGGTCAGGCTGGAACTGAGGAG21410.2152055107085096No Hit
GGCGTAGAGTTTGTGTTTCTCGTAGTCTGCTTTGCTCAGCGTCAGGGTGC20910.21017969308336926No Hit
CTTTTGGCCAGGGGACCAAGCTGGAGATCAAACGAACTGTGGCTGCACCA20680.2078678169758047No Hit
CAACAGAGGCAGTTCCAGATTTCAACTGCTCATCAGATGGCGGGAAGATG20270.20374664652318963No Hit
GTCCTGCTCTGTGACACTCTCCTGGGAGTTACCCGATTGGAGGGCGTTATCCACCTTCCACTGTACTTTGGCCT19980.20083167230060822No Hit
GGATGGGAGGGGGTCAGGCTGGAACTGAGGAGCAGGTGGGGGCACTTCTCCCTCTAACACTCTCCCCTGTTGAAG19930.20032909053809422No Hit
GATCAGGACTCCTCAGTTCACCTTCTCACAATGAGGCTCCCTGCTCAGCTCCTGGGGCTGCTAATGCTCTGGGT19160.19258933139537807No Hit
GTTCTAGAGCTTGCATGCAGTAGTAAATCCCAACATCCTCAGCCTCCACT18580.18675938295021527No Hit
GTACAGTGGAAGGTGGATAACGCCCTCCAATCGGGTAACTCCCAGGAGAG17580.17670774769993455No Hit
GTGTAGAGAATACCATCCATTCCTATGCCAGAGGTTCTCACTAGACCTGC17570.17660723134743175No Hit
GGACTCCTCAGTTCACCTTCTCACAATGAGGCTCCCTGCTCAGCTCCTGG17360.1744963879448728No Hit
GCGTCAGGGTGCTGCTGAGGCTGTAGGTGCTGTCCTTGCTGTCCTGCTCT17360.1744963879448728No Hit
GAGCAGGACAGCAAGGACAGCACCTACAGCCTCAGCAGCACCCTGACGCT16720.16806334138469317No Hit
GTCTTCATCTTCCCGCCATCTGATGAGCAGTTGAAATCTGGAACTGCCTCTGTTGTGTGCCTGCTGAATAACTT16150.16233390929203317No Hit
CTCTGGGATAGAAGTTATTCAGCAGGCACACAACAGAGGCAGTTCCAGAT15440.1551972482643339No Hit
CTCTACGCCTGCGAAGTCACCCATCAGGGCCTGAGCTCGCCCGTCACAAA15410.15489569920682544No Hit
GGCCAAAAGTGTACGGAGGTTCTAGAGCTTGCATGCAGTAGTAAATCCCA15270.15348847027178616No Hit
CCACTGTACTTTGGCCTCTCTGGGATAGAAGTTATTCAGCAGGCACACAACAGAGGCAGTTCCAGATTTCAACTG14840.14916626711416547No Hit
GTGGCTGCACCATCTGTCTTCATCTTCCCGCCATCTGATGAGCAGTTGAAATCTGGAACTGCCTCTGTTGTGTGC14410.14484406395654478No Hit
GGCCAAAGGATGGGAGGGGGTCAGGCTGGAACTGAGGAGCAGGTGGGGGCACTTCTCCCTCTAACACTCTCCC14160.1423311551439746No Hit
GTGCAGCCACAGTTCGTTTGATCTCCAGCTTGGTCCCCTGGCCAAAAGTG13890.1396172136263988No Hit
ATCCCAGAGAGGCCAAAGTACAGTGGAAGGTGGATAACGCCCTCCAATCG13420.13489294505876687No Hit
GGCTGTAGGTGCTGTCCTTGCTGTCCTGCTCTGTGACACTCTCCTGGGAGTTACCCGATTGGAGGGCGTTATCCA13410.13479242870626407No Hit
GATGAAGACAGATGGTGCAGCCACAGTTCGTTTGATCTCCAGCTTGGTCCCCTGGCCAAAAGTGTACGGAGGTT12650.12715318591605074No Hit
GGCCCTGATGGGTGACTTCGCAGGCGTAGAGTTTGTGTTTCTCGTAGTCTGCTTTGCTCAGCGTCAGGGTGCTG12640.12705266956354794No Hit
GTAGTAAATCCCAACATCCTCAGCCTCCACTCTGCTGATTCTCAATGTAA12630.12695215321104514No Hit
CTTATACACATCTCCGAGCCCACGAGACTAAGGCGAATCTCGTATGCCGT12450.1251428588659946No Hit
CTCCAGGGGTGACGGGCAGGGAGAGTGGAGACTGAGTCATCACAGTGTCC12210.12273046640592725No Hit
GTCTCCACTCTCCCTGCCCGTCACCCCTGGAGAGTCGGCCTCCATCTCCT12020.12082065570837393No Hit
GCTTTGCTCAGCGTCAGGGTGCTGCTGAGGCTGTAGGTGCTGTCCTTGCT12020.12082065570837393No Hit
GAGTTTATTCAGGGGTGGGGACAGGCGGGTGGCTCAGTAGCAGGTGCCGT11880.11941342677333462No Hit
GTATTCTCTACACTGGTACCTACAGCAGCCAGGGCAGCCTCCACGACTCCTGATCTACAAGACTTTTAATCGGGC11840.11901136136332338No Hit
CAATGAGGCTCCCTGCTCAGCTCCTGGGGCTGCTAATGCTCTGGGTCTCTGGATCCAGTGGGGACACTGTGATGA11700.11760413242828409No Hit
CCTCAGTTCACCTTCTCACAATGAGGCTCCCTGCTCAGCTCCTGGGGCTG11670.11730258337077568No Hit
GTTGAAGCTCTTTGTGACGGGCGAGCTCAGGCCCTGATGGGTGACTTCGC11640.11700103431326726No Hit
GTTGTGTGCCTGCTGAATAACTTCTATCCCAGAGAGGCCAAAGTACAGTG11440.11499070726321112No Hit
GGTACCTACAGCAGCCAGGGCAGCCTCCACGACTCCTGATCTACAAGACT11420.11478967455820552No Hit
GCTAATGCTCTGGGTCTCTGGATCCAGTGGGGACACTGTGATGACTCAGTCTCCACTCTCCCTGCCCGTCACCC11230.11287986386065217No Hit
GGTCTAGTGAGAACCTCTGGCATAGGAATGGATGGTATTCTCTACACTGGTACCTACAGCAGCCAGGGCAGCCTC11070.11127160222060727No Hit
GTATGCTGGTCACAGCGAAGGTGGTGGTGCCCTGGCTGGGCTCCTGCCGG10930.10986437328556796No Hit
GTCCTTGCTGTCCTGCTCTGTGACACTCTCCTGGGAGTTACCCGATTGGAGGGCGTTATCCACCTTCCACTGTA10900.10956282422805955No Hit
ATTCAGGGGTGGGGACAGGCGGGTGGCTCAGTAGCAGGTGCCGTCCACCT10880.10936179152305392No Hit
TCCTTGGGGCTGAAGCCACGTGCCAGGCACGTCAGCGTCACCAGCTCGTT10720.10775352988300901No Hit
GGTTTAGTGGCAGTGGATCAGGCACATATTTTACATTGAGAATCAGCAGA10620.10674836635798095No Hit
GCTCCTGGGGCTGCTAATGCTCTGGGTCTCTGGATCCAGTGGGGACACTGTGATGACTCAGTCTCCACTCTCC10490.10544165377544445No Hit
GTGGAAAAAGGGTCAGAGGCCAAAGGATGGGAGGGGGTCAGGCTGGAACTGAGGAGCAGGTGGGGGCACTTCTCC10480.10534113742294166No Hit
GTACTTTGGCCTCTCTGGGATAGAAGTTATTCAGCAGGCACACAACAGAGGCAGTTCCAGATTTCAACTGCTCAT10310.10363235943039394No Hit
CCTCCAACATTAGCATAATTAAAGCCAAGGAGGAGGAGGGGGGTGAGGTG10130.1018230650853434No Hit
GGTGAAAGATGAGCTGGAGGACCGCAATAGGGGTAGGTCCCCTGTGGAAA10100.10152151602783499No Hit
TTACTACTGCATGCAAGCTCTAGAACCTCCGTACACTTTTGGCCAGGGGA10000.10051635250280691No Hit

[OK]Adapter Content

Adapter graph

Produced by FastQC (version 0.11.9)