FastQCFastQC Report
Wed 27 Apr 2022
EGAF00004684399

Summary

[OK]Basic Statistics

MeasureValue
FilenameEGAF00004684399
File typeConventional base calls
EncodingSanger / Illumina 1.9
Total Sequences994863
Sequences flagged as poor quality0
Sequence length20-76
%GC54

[OK]Per base sequence quality

Per base quality graph

[OK]Per tile sequence quality

Per tile quality graph

[OK]Per sequence quality scores

Per Sequence quality graph

[FAIL]Per base sequence content

Per base sequence content

[FAIL]Per sequence GC content

Per sequence GC content graph

[OK]Per base N content

N content graph

[WARN]Sequence Length Distribution

Sequence length distribution

[FAIL]Sequence Duplication Levels

Duplication level graph

[WARN]Overrepresented sequences

SequenceCountPercentagePossible Source
CTCCTGGGAGTTACCCGATTGGAGGGCGTTATCCACCTTCCACTGTACTTTGGCCTCTCTGGGATAGAAGTTATT33910.34085095133701826No Hit
CTCTAACACTCTCCCCTGTTGAAGCTCTTTGTGACGGGCGAGCTCAGGCC31520.3168275430888474No Hit
GGGGGGGGGGGGGGGGGGGGGGGGGGGGGGGGGGGGGGGGGGGGGGGGGG28460.2860695392229885No Hit
CTCCCAGGAGAGTGTCACAGAGCAGGACAGCAAGGACAGCACCTACAGCC24350.24475731834433484No Hit
CTCTAGAACCTCCGTACACTTTTGGCCAGGGGACCAAGCTGGAGATCAAA23240.23360000321652327No Hit
GGCTGGAACTGAGGAGCAGGTGGGGGCACTTCTCCCTCTAACACTCTCCC22310.22425198243376226No Hit
CCCCTGGAGAGTCGGCCTCCATCTCCTGCAGGTCTAGTGAGAACCTCTGG22070.22183958997369488No Hit
GTCCTGCTCTGTGACACTCTCCTGGGAGTTACCCGATTGGAGGGCGTTATCCACCTTCCACTGTACTTTGGCCT21120.21229053648592822No Hit
TCTCTACACTGGTACCTACAGCAGCCAGGGCAGCCTCCACGACTCCTGAT20830.20937556226334683No Hit
GTTCTAGAGCTTGCATGCAGTAGTAAATCCCAACATCCTCAGCCTCCACT19810.19912289430806052No Hit
CTTTTGGCCAGGGGACCAAGCTGGAGATCAAACGAACTGTGGCTGCACCA19350.19449914209293137No Hit
GATCAGGACTCCTCAGTTCACCTTCTCACAATGAGGCTCCCTGCTCAGCTCCTGGGGCTGCTAATGCTCTGGGT18670.18766403012274052No Hit
CAACAGAGGCAGTTCCAGATTTCAACTGCTCATCAGATGGCGGGAAGATG18580.18675938295021527No Hit
GGCGTAGAGTTTGTGTTTCTCGTAGTCTGCTTTGCTCAGCGTCAGGGTGC18440.18535215401517596No Hit
CAATAGGGGTAGGTCCCCTGTGGAAAAAGGGTCAGAGGCCAAAGGATGGGAGGGGGTCAGGCTGGAACTGAGGAG16750.1683648904422016No Hit
GGATGGGAGGGGGTCAGGCTGGAACTGAGGAGCAGGTGGGGGCACTTCTCCCTCTAACACTCTCCCCTGTTGAAG16730.16816385773719597No Hit
GAGCAGGACAGCAAGGACAGCACCTACAGCCTCAGCAGCACCCTGACGCT16600.1668571451546595No Hit
GTGTAGAGAATACCATCCATTCCTATGCCAGAGGTTCTCACTAGACCTGC16530.16615353068713984No Hit
GTACAGTGGAAGGTGGATAACGCCCTCCAATCGGGTAACTCCCAGGAGAG16310.16394217093207808No Hit
GCGTCAGGGTGCTGCTGAGGCTGTAGGTGCTGTCCTTGCTGTCCTGCTCT16170.16253494199703877No Hit
GTCTTCATCTTCCCGCCATCTGATGAGCAGTTGAAATCTGGAACTGCCTCTGTTGTGTGCCTGCTGAATAACTT15140.15218175768924969No Hit
CTCTGGGATAGAAGTTATTCAGCAGGCACACAACAGAGGCAGTTCCAGAT15050.1512771105167244No Hit
GGACTCCTCAGTTCACCTTCTCACAATGAGGCTCCCTGCTCAGCTCCTGG14940.15017143063919353No Hit
GGCCAAAAGTGTACGGAGGTTCTAGAGCTTGCATGCAGTAGTAAATCCCA14470.1454471620715616No Hit
CCCTGACGCTGAGCAAAGCAGACTACGAGAAACACAAACTCTACGCCTGCGAAGTCACCCATCAGGGCCTGAGCT14300.14373838407901388No Hit
CTCTACGCCTGCGAAGTCACCCATCAGGGCCTGAGCTCGCCCGTCACAAA13940.14011979538891284No Hit
GTGGCTGCACCATCTGTCTTCATCTTCCCGCCATCTGATGAGCAGTTGAAATCTGGAACTGCCTCTGTTGTGTGC13790.13861205010137073No Hit
GTGCAGCCACAGTTCGTTTGATCTCCAGCTTGGTCCCCTGGCCAAAAGTG13560.13630017399380617No Hit
CCACTGTACTTTGGCCTCTCTGGGATAGAAGTTATTCAGCAGGCACACAACAGAGGCAGTTCCAGATTTCAACTG12820.12886196390859847No Hit
GGCTGTAGGTGCTGTCCTTGCTGTCCTGCTCTGTGACACTCTCCTGGGAGTTACCCGATTGGAGGGCGTTATCCA12750.12815834944107882No Hit
GTAGTAAATCCCAACATCCTCAGCCTCCACTCTGCTGATTCTCAATGTAA12740.12805783308857602No Hit
GATGAAGACAGATGGTGCAGCCACAGTTCGTTTGATCTCCAGCTTGGTCCCCTGGCCAAAAGTGTACGGAGGTT12510.12574595698101146No Hit
CTCCAGGGGTGACGGGCAGGGAGAGTGGAGACTGAGTCATCACAGTGTCC12220.12283098275843005No Hit
ATCCCAGAGAGGCCAAAGTACAGTGGAAGGTGGATAACGCCCTCCAATCG12100.12162478652839638No Hit
GGCCAAAGGATGGGAGGGGGTCAGGCTGGAACTGAGGAGCAGGTGGGGGCACTTCTCCCTCTAACACTCTCCC11880.11941342677333462No Hit
GGTCTAGTGAGAACCTCTGGCATAGGAATGGATGGTATTCTCTACACTGGTACCTACAGCAGCCAGGGCAGCCTC11800.11860929595331217No Hit
GTATTCTCTACACTGGTACCTACAGCAGCCAGGGCAGCCTCCACGACTCCTGATCTACAAGACTTTTAATCGGGC11770.11830774689580374No Hit
CCTACAGCCTCAGCAGCACCCTGACGCTGAGCAAAGCAGACTACGAGAAACACAAACTCTACGCCTGCGAAGTCA11610.11669948525575884No Hit
CCTCAGTTCACCTTCTCACAATGAGGCTCCCTGCTCAGCTCCTGGGGCTG11580.11639793619825042No Hit
GAGTTTATTCAGGGGTGGGGACAGGCGGGTGGCTCAGTAGCAGGTGCCGT11480.11539277267322233No Hit
GTTGTGTGCCTGCTGAATAACTTCTATCCCAGAGAGGCCAAAGTACAGTG11030.11086953681059603No Hit
GGTTTAGTGGCAGTGGATCAGGCACATATTTTACATTGAGAATCAGCAGA10820.1087586934080371No Hit
GTCTCCACTCTCCCTGCCCGTCACCCCTGGAGAGTCGGCCTCCATCTCCT10750.10805507894051744No Hit
CAATGAGGCTCCCTGCTCAGCTCCTGGGGCTGCTAATGCTCTGGGTCTCTGGATCCAGTGGGGACACTGTGATGA10730.10785404623551181No Hit
GTCCTTGCTGTCCTGCTCTGTGACACTCTCCTGGGAGTTACCCGATTGGAGGGCGTTATCCACCTTCCACTGTA10720.10775352988300901No Hit
GGCCCTGATGGGTGACTTCGCAGGCGTAGAGTTTGTGTTTCTCGTAGTCTGCTTTGCTCAGCGTCAGGGTGCTG10640.10694939906298656No Hit
GCTCCTGGGGCTGCTAATGCTCTGGGTCTCTGGATCCAGTGGGGACACTGTGATGACTCAGTCTCCACTCTCC10490.10544165377544445No Hit
GCTAATGCTCTGGGTCTCTGGATCCAGTGGGGACACTGTGATGACTCAGTCTCCACTCTCCCTGCCCGTCACCC10380.10433597389791359No Hit
GTACTTTGGCCTCTCTGGGATAGAAGTTATTCAGCAGGCACACAACAGAGGCAGTTCCAGATTTCAACTGCTCAT10170.10222513049535464No Hit
GTGTCACAGAGCAGGACAGCAAGGACAGCACCTACAGCCTCAGCAGCACC10010.10061686885530974No Hit

[OK]Adapter Content

Adapter graph

Produced by FastQC (version 0.11.9)