FastQCFastQC Report
Sat 23 Apr 2022
EGAF00004684401

Summary

[OK]Basic Statistics

MeasureValue
FilenameEGAF00004684401
File typeConventional base calls
EncodingSanger / Illumina 1.9
Total Sequences705872
Sequences flagged as poor quality0
Sequence length20-76
%GC55

[OK]Per base sequence quality

Per base quality graph

[OK]Per tile sequence quality

Per base quality graph

[OK]Per sequence quality scores

Per Sequence quality graph

[FAIL]Per base sequence content

Per base sequence content

[FAIL]Per sequence GC content

Per sequence GC content graph

[OK]Per base N content

N content graph

[WARN]Sequence Length Distribution

Sequence length distribution

[FAIL]Sequence Duplication Levels

Duplication level graph

[WARN]Overrepresented sequences

SequenceCountPercentagePossible Source
GTAATACACGGCCGTGTCCTCAGATCTCAGGCTGCTCAGCTCCATGTAGGCTGCGTCTGTAGATGTGTCCTCGGT24960.35360518620939774No Hit
GTATTACTGTCAGCAGTATGGTAGCTCACCTCAACTCACTTTCGGCGGAGGGACCAAGGTGGAGATCAAACGAA24450.34638008024117684No Hit
GGGGGGGGGGGGGGGGGGGGGGGGGGGGGGGGGGGGGGGGGGGGGGGGGG23240.3292381621597117No Hit
CTCCTGGGAGTTACCCGATTGGAGGGCGTTATCCACCTTCCACTGTACTTTGGCCTCTCTGGGATAGAAGTTATT18750.26562889589047306No Hit
CTCTAACACTCTCCCCTGTTGAAGCTCTTTGTGACGGGCGAGCTCAGGCC15820.22411995375932178No Hit
GTCTGGGGCTGAGGTGAAGGAGCCTGGGGCCTCAGTGAAGGTCTCCTGCAAGGTTTCCGGATACACCCTCACTGA14680.20796971688918103No Hit
GTGTATTACTGTGCAACCCATCGCCCCATTTACGGTCCTACTTACACTAT13850.19621121109776274No Hit
CAGTAATACACTGCAAAATCTTCAGGCTCCAGTCTGCTGATGGTGAGAGTGAAGTCTGTCCCAGACCCACTGCCA13230.18742774894031777No Hit
CTCCCAGGAGAGTGTCACAGAGCAGGACAGCAAGGACAGCACCTACAGCC13160.18643606772899335No Hit
GTTAGGACCCAGAGGGAACCATGGAAACCCCAGCGCAGCTTCTCTTCCTCCTGCTACTCTGGCTCCCAGATACCA12420.17595258063784935No Hit
GGCGTAGACTTTGTGTTTCTCGTAGTCTGCTTTGCTCAGCGTCAGGGTGC12340.17481923068205No Hit
GGCTGGAACTGAGGAGCAGGTGGGGGCACTTCTCCCTCTAACACTCTCCC11100.15725230636716006No Hit
GTGTAAGTAGGACCGTAAATGGGGCGATGGGTTGCACAGTAATACACGGC11030.1562606251558356No Hit
GTCCTGCTCTGTGACACTCTCCTGGGAGTTACCCGATTGGAGGGCGTTATCCACCTTCCACTGTACTTTGGCCT10620.15045220663236394No Hit
GTGTATTACTGTCAGCAGTATGGTAGCTCACCTCAACTCACTTTCGGCGG10530.14917718793208967No Hit
GTGTGACCTGGAGCGAAAGCGGACAGGGCGTGACCGCCAGAAACTTCCCACCCAGCCAGGATGCCTCCGGGGAC10380.14705215676496589No Hit
GTCACATCCTGGCTGGGATTCGTGTAGTGCTTCACGTGGCATGTCACGGA10180.14421878187546752No Hit
GTGTACAGGTCCCCGGAGGCATCCTGGCTGGGTGGGAAGTTTCTGGCGGT10030.14209375070834374No Hit
GTATGGTAGCTCACCTCAACTCACTTTCGGCGGAGGGACCAAGGTGGAGATCAAACGAACTGTGGCTGCACCAT9820.1391187070743704No Hit
GTCTACGCCTGCGAAGTCACCCATCAGGGCCTGAGCTCGCCCGTCACAAA9650.1367103384182968No Hit
CAATAGGGGTAGGTCCCCTGTGGAAAAAGGGTCAGAGGCCAAAGGATGGGAGGGGGTCAGGCTGGAACTGAGGAG9500.13458530725117301No Hit
GTCTGGGACAGACTTCACTCTCACCATCAGCAGACTGGAGCCTGAAGATT9470.13416030101774826No Hit
CAACAGAGGCAGTTCCAGATTTCAACTGCTCATCAGATGGCGGGAAGATG9360.13260194482852414No Hit
GTTCAAGGACCACCTGAGCGTGACCTCTGTGGCTGCTACAGCGTGTCCAG9260.13118525738377496No Hit
AGGTCACACTGAGTGGCTCCTGGGGGAAGAAGCCCTGGACCAGGCAGGCG8700.1232518076931795No Hit
CTCCTGCAGGGCCAGTCAGAGTGTTAGCAGCAGCTTCTTAGCCTGGTACC8670.12282680145975473No Hit
GCGTCAGGGTGCTGCTGAGGCTGTAGGTGCTGTCCTTGCTGTCCTGCTCT8600.12183512024843031No Hit
GAGCAGGACAGCAAGGACAGCACCTACAGCCTCAGCAGCACCCTGACGCT8590.1216934515039554No Hit
GGGTGCGACAGGCTCCTGGAAAAGGGCTTGAGTGGATGGGAAGTTTTGATCCTGAAGAAGGTGAAACAATCTACG8540.1209851077815808No Hit
GAGTTTATTCAGGGGTGGGGACAGGCGGGCGGCTCAGTAGCAGGTGCCGT8520.12070177029263095No Hit
GTCCAGGGCTTCTTCCCCCAGGAGCCACTCAGTGTGACCTGGAGCGAAAGCGGACAGGGCGTGACCGCCAGAAA8510.12056010154815604No Hit
CATCTATGGTGCATCCAGAAGGGCCACTGGCATCCCAGACAGGTTCAGTGGCAGTGGGTCTGGGACAGACTTCA8500.12041843280368111No Hit
GGATGGGAGGGGGTCAGGCTGGAACTGAGGAGCAGGTGGGGGCACTTCTCCCTCTAACACTCTCCCCTGTTGAAG8480.1201350953147313No Hit
GTAATACACTGCAAAATCTTCAGGCTCCAGTCTGCTGATGGTGAGAGTGAAGTCTGTCCCAGACCCACTGCCA8410.11914341410340684No Hit
GTACAGTGGAAGGTGGATAACGCCCTCCAATCGGGTAACTCCCAGGAGAG8330.11801006414760751No Hit
GCTCAGTAGCAGGTGCCGTCCACCTCCGCCATGACAACAGACACATTGAC8240.11673504544733322No Hit
TCCATGCACTGGGTGCGACAGGCTCCTGGAAAAGGGCTTGAGTGGATGGG8180.11588503298048372No Hit
GTGCAGTGACAGTCGGGGGTGGCAGCATGAGGGAGATGGGGTAGGTGGAG8140.11531835800258404No Hit
GTCTTTGTCTCCAGGGGAAAGAGCCACCCTCTCCTGCAGGGCCAGTCAGA7950.11262665185756057No Hit
GCCCCAAGGTCTTCCCGCTGAGCCTCTGCAGCACCCAGCCAGATGGGAAC7910.11205997687966089No Hit
TACTTACACTATGGACGTCTGGGGCCAAGGGACCTCGGTCACCGTCTCCTCAGCATCCCCGACCAGCCCCAAGGT7860.1113516331572863No Hit
CTCTGGGATAGAAGTTATTCAGCAGGCACACAACAGAGGCAGTTCCAGAT7710.10922660199016251No Hit
GTCTCCAGGCACCCTGTCTTTGTCTCCAGGGGAAAGAGCCACCCTCTCCTGCAGGGCCAGTCAGAGTGTTAGCAG7670.10865992701226285No Hit
GTATTACTGTGCAACCCATCGCCCCATTTACGGTCCTACTTACACTATGGACGTCTGGGGCCAAGGGACCTCGGT7450.10554321463381462No Hit
CCCTGACGCTGAGCAAAGCAGACTACGAGAAACACAAAGTCTACGCCTGCGAAGTCACCCATCAGGGCCTGAGCT7380.1045515334224902No Hit
GTGCATGGATAAGTCAGTGAGGGTGTATCCGGAAACCTTGCAGGAGACCTTCACTGAGGCCCCAGGCTCCTTCAC7380.1045515334224902No Hit
GACTTATCCATGCACTGGGTGCGACAGGCTCCTGGAAAAGGGCTTGAGTG7280.103134845977741No Hit

[OK]Adapter Content

Adapter graph

[WARN]Kmer Content

Kmer graph

SequenceCountPValueObs/Exp MaxMax Obs/Exp Position
TACGTAT451.6949819E-460.02551770
TTTGACG200.00649882353.4001757
GGTATCA8700.046.0487061
TCGGACG306.8716274E-445.2104954
GTACGTA707.851328E-637.3036869
GTATCAA22350.037.217161
TGGTACG501.195743E-437.19352767
GCTTATC656.1761784E-736.562791
GGTACGT551.6355175E-435.26464568
CGGGTAT502.0573329E-433.9150776
TGCGCGA400.00282265333.91507710
TATCAAC24850.033.4302942
ACGGGAC751.899889E-631.66753616
TACTCCG652.6091568E-531.3040085
AACGCAG26500.031.0994856
ATCAACG26500.031.0906733
TAGTTAT450.004544181730.7725242
TCAACGC26850.030.5612814
CAACGCA26950.030.45225
ACGCAGA28150.029.3970877
Produced by FastQC (version 0.11.5)