FastQCFastQC Report
Sat 23 Apr 2022
EGAF00004684402

Summary

[OK]Basic Statistics

MeasureValue
FilenameEGAF00004684402
File typeConventional base calls
EncodingSanger / Illumina 1.9
Total Sequences631556
Sequences flagged as poor quality0
Sequence length20-76
%GC54

[OK]Per base sequence quality

Per base quality graph

[OK]Per tile sequence quality

Per base quality graph

[OK]Per sequence quality scores

Per Sequence quality graph

[FAIL]Per base sequence content

Per base sequence content

[FAIL]Per sequence GC content

Per sequence GC content graph

[OK]Per base N content

N content graph

[WARN]Sequence Length Distribution

Sequence length distribution

[FAIL]Sequence Duplication Levels

Duplication level graph

[WARN]Overrepresented sequences

SequenceCountPercentagePossible Source
CTCCTGGGAGTTACCCGATTGGAGGGCGTTATCCACCTTCCACTGTACTTTGGCCTCTCTGGGATAGAAGTTATT20560.32554516147420026No Hit
CTCTAACACTCTCCCCTGTTGAAGCTCTTTGTGACGGGCGAGCTCAGGCC17190.2721848893843143No Hit
GTTAGGACCCAGAGGGAACCATGGAAACCCCAGCGCAGCTTCTCTTCCTCCTGCTACTCTGGCTCCCAGATACCA15160.24004205486132663No Hit
ACCCTGGCCAGGCTCCCAGGCTCCTCATCTATGGTGCATCCAGCAGGGCC13250.20979928937418058No Hit
GGCGTAGACTTTGTGTTTCTCGTAGTCTGCTTTGCTCAGCGTCAGGGTGC13190.20884925485625977No Hit
CAACAGAGGCAGTTCCAGATTTCAACTGCTCATCAGATGGCGGGAAGATG12390.19618212795064885No Hit
GGCTGGAACTGAGGAGCAGGTGGGGGCACTTCTCCCTCTAACACTCTCCC11810.18699846094408099No Hit
GAAATACACAGCCGAGTCCGCGGCGTTCACAGAGCTCAACTTCAGGGAGA11740.18589008733984003No Hit
CATCTATGGTGCATCCAGCAGGGCCACTGGCATCCCAGACAGGTTCAGTGGCAGTGGCTCTGGGACAGACTTCA10830.17148123048470762No Hit
GTGCAGTGACAGTCGGGGGTGGCAGCATGAGGGAGATGGGGTAGGTGGAG10820.17132289139838747No Hit
GTCTCCAGGCACCCTGTCTTTGTCTCCAGGGGAAAGAGCCACCCTCTCCTGCAGGGCCAGTCAGAGTGTTAGCAG10780.17068953505310694No Hit
GCGTCAGGGTGCTGCTGAGGCTGTAGGTGCTGTCCTTGCTGTCCTGCTCT10420.16498932794558202No Hit
CAATAGGGGTAGGTCCCCTGTGGAAAAAGGGTCAGAGGCCAAAGGATGGGAGGGGGTCAGGCTGGAACTGAGGAG10390.16451431068662162No Hit
GTCCTGCTCTGTGACACTCTCCTGGGAGTTACCCGATTGGAGGGCGTTATCCACCTTCCACTGTACTTTGGCCT10360.16403929342766121No Hit
CTCCTGCAGGGCCAGTCAGAGTGTTAGCAGCAGCTACTTAGCCTGGTACC10170.16103085078757862No Hit
GTCACTGCACCGACCGGCCCTCGAGGACCTGCTCTTAGGTTCAGAAGCGAACCTCACGTGCACACTGACCGGC9950.15754739088853562No Hit
GTATGCTGGTCACAGCGAAGGTGGTGGTGCCCTGGCTGGGCTCCTGCCGG9700.1535889137305322No Hit
CTCCCAGGAGAGTGTCACAGAGCAGGACAGCAAGGACAGCACCTACAGCC9420.14915541931356838No Hit
GGATGGGAGGGGGTCAGGCTGGAACTGAGGAGCAGGTGGGGGCACTTCTCCCTCTAACACTCTCCCCTGTTGAAG9420.14915541931356838No Hit
GTCACATCCTGGCTGGGATTCGTGTAGTGCTTCACGTGGCATGTCACGGA9210.14583029850084553No Hit
TCCTTGGGGCTGAAGCCACGTGCCAGGCACGTCAGCGTCACCAGCTCGTT9080.14377189037868376No Hit
GTTTTACTGTCAGCAATATGGTAGTTCACCCTACACTTTTGGCCAGGGGA8810.13949673504804008No Hit
AGGTCACACTGAGTGGCTCCTGGGGGAAGAAGCCCTGGACCAGGCAGGCG8770.13886337870275953No Hit
CTCCAGGCACCCTGTCTTTGTCTCCAGGGGAAAGAGCCACCCTCTCCTGC8760.1387050396164394No Hit
CTCTGGGATAGAAGTTATTCAGCAGGCACACAACAGAGGCAGTTCCAGAT8690.13759666601219844No Hit
GCTCAGTAGCAGGTGCCGTCCACCTCCGCCATGACAACAGACACATTGAC8600.13617161423531723No Hit
CCACTGTACTTTGGCCTCTCTGGGATAGAAGTTATTCAGCAGGCACACAACAGAGGCAGTTCCAGATTTCAACTG8460.13395486702683532No Hit
GTCTTTGTCTCCAGGGGAAAGAGCCACCCTCTCCTGCAGGGCCAGTCAGA8450.13379652794051516No Hit
GTCTACGCCTGCGAAGTCACCCATCAGGGCCTGAGCTCGCCCGTCACAAA8440.13363818885419504No Hit
ATATGGTAGTTCACCCTACACTTTTGGCCAGGGGACCAAGCTGGAGATCAAACGAACTGTGGCTGCACCAT8360.13237147616363396No Hit
GTGTACAGGTCCCCGGAGGCATCCTGGCTGGGTGGGAAGTTTCTGGCGGT8320.1317381198183534No Hit
GACTTCACTCTCACCATCAGCAGACTGGAGCCTGAAGACTTTGCTGTGTTTTACTGTCAGCAATATGGTAGTTCA8260.13078808530043257No Hit
GAGTTTATTCAGGGGTGGGGACAGGCGGGCGGCTCAGTAGCAGGTGCCGT8250.13062974621411244No Hit
GTGTATTTCTGTGCGAGAGGCCCGTTTTTATATGGTTCGGGGAGACCTCCGTTTGACTCCTGGGGCCAGGGAACC7960.12603791271082848No Hit
GTTCAAGGACCACCTGAGCGTGACCTCTGTGGCTGCTACAGCGTGTCCAG7960.12603791271082848No Hit
GTCATGGACCTCCTGCACAAGAACATGAAACACCTGTGGTTCTTCCTCCTCCTGGTGGCAGCTCCCAGATGGGTC7930.12556289545186808No Hit
GTACAGTGGAAGGTGGATAACGCCCTCCAATCGGGTAACTCCCAGGAGAG7780.12318780915706604No Hit
GGGTTGTGCTGGTACCAGGCTAAGTAGCTGCTGCTAACACTCTGACTGGCCCTGCAGGAGAGGGTGGCTCTTTCC7740.12255445281178548No Hit
GATGAAGACAGATGGTGCAGCCACAGTTCGTTTGATCTCCAGCTTGGTCC7630.120812722862264No Hit
CTTATACACATCTCCGAGCCCACGAGACAAGAGGCAATCTCGTATGCCGT7600.12033770560330359No Hit
GTCTTCATCTTCCCGCCATCTGATGAGCAGTTGAAATCTGGAACTGCCTCTGTTGTGTGCCTGCTGAATAACTT7560.11970434925802304No Hit
GTGCAGCCACAGTTCGTTTGATCTCCAGCTTGGTCCCCTGGCCAAAAGTG7530.11922933199906263No Hit
GTCTTGGACTCGGGGTAGGCAGCAGTGCAAGTGAAGGTCTTCCCATGGTT7400.11717092387690087No Hit
GTGGCTGCACCATCTGTCTTCATCTTCCCGCCATCTGATGAGCAGTTGAAATCTGGAACTGCCTCTGTTGTGTGC7290.11542919392737935No Hit
GTCAGAGTGTTAGCAGCAGCTACTTAGCCTGGTACCAGCACAACCCTGGC7270.11511251575473909No Hit
CAGTTAGGACCCAGAGGGAACCATGGAAACCCCAGCGCAGCTTCTCTTCCTCCTGCTACTCTGGCTCCCAGATAC7220.11432082032313841No Hit
GCTCAGTTAGGACCCAGAGGGAACCATGGAAACCCCAGCGCAGCTTCTCT7140.11305410763257732No Hit
CTACTGGACCTGGATCCGCCAGTCCCCAGGGAAGGGGCCGGAGTGGATTGGGGAAATCAGTCACAGTGGAATCAC7110.11257909037361691No Hit
GCACAACCCTGGCCAGGCTCCCAGGCTCCTCATCTATGGTGCATCCAGCAGGGCCACTGGCATCCCAGACAGGTT7100.11242075128729677No Hit
ATTCAGGGGTGGGGACAGGCGGGCGGCTCAGTAGCAGGTGCCGTCCACCT7080.11210407311465649No Hit
GTGTGCACGTGAGGTTCGCTTCTGAACCTAAGAGCAGGTCCTCGAGGGCC7080.11210407311465649No Hit
GGCTGTAGGTGCTGTCCTTGCTGTCCTGCTCTGTGACACTCTCCTGGGAGTTACCCGATTGGAGGGCGTTATCCA7020.11115403859673569No Hit
CCATAGATGAGGAGCCTGGGAGCCTGGCCAGGGTTGTGCTGGTACCAGGCTAAGTAGCTGCTGCTAACACTCTGA7020.11115403859673569No Hit
GGATGTGACTGTGCCCTGCCCAGTTCCCTCAACTCCACCTACCCCATCTCCCTCAACTCCACCTACCCCATCTCC6960.11020400407881485No Hit
GTGTAGGGTGAACTACCATATTGCTGACAGTAAAACACAGCAAAGTCTTC6880.10893729138825377No Hit
GCCCCAAGGTCTTCCCGCTGAGCCTCTGCAGCACCCAGCCAGATGGGAAC6830.10814559595665309No Hit
CAGTAGACCCGTCCAAGGACCAGTTCTCCCTGAAGTTGAGCTCTGTGAACGCCGCGGACTCGGCTGTGTATTT6810.10782891778401281No Hit
CGTGCACACTGACCGGCCTGAGAGATGCCTCAGGTGTCACCTTCACCTGGACGCCCTCAAGTGGGAAGAGCG6760.10703722235241213No Hit
GTGTGACCTGGAGCGAAAGCGGACAGGGCGTGACCGCCAGAAACTTCCCACCCAGCCAGGATGCCTCCGGGGAC6660.10545383148921077No Hit
CAGTATGCTGGTCACAGCGAAGGTGGTGGTGCCCTGGCTGGGCTCCTGCC6650.10529549240289064No Hit
GTGTTAGCAGCAGCTACTTAGCCTGGTACCAGCACAACCCTGGCCAGGCTCCCAGGCTCCTCATCTATGGTGCAT6610.10466213605761009No Hit
GTGCCTAGCCGGCAAGTCCGTGACATGCCACGTGAAGCACTACACGAATCCCAGCCAGGATGTGACTGTGCCCTG6560.1038704406260094No Hit
ATCCCAGAGAGGCCAAAGTACAGTGGAAGGTGGATAACGCCCTCCAATCG6500.1029204061080886No Hit
GAGTGAAGTCTGTCCCAGAGCCACTGCCACTGAACCTGTCTGGGATGCCA6490.10276206702176846No Hit
GGCCAAAAGTGTAGGGTGAACTACCATATTGCTGACAGTAAAACACAGCA6420.1016536934175275No Hit
GTACTTTGGCCTCTCTGGGATAGAAGTTATTCAGCAGGCACACAACAGAGGCAGTTCCAGATTTCAACTGCTCAT6420.1016536934175275No Hit
GGTCTACTGATATGGTGACTCGACTCTTGAGTGACGGGTTGTAGCTGGTG6370.10086199798592682No Hit
GAGTGGCTCCTGGGGGAAGAAGCCCTGGACCAGGCAGGCGATGACCACGT6350.10054531981328654No Hit
CAGTAAAACACAGCAAAGTCTTCAGGCTCCAGTCTGCTGATGGTGAGAGTGAAGTCTGTCCCAGAGCCACTGCCA6330.10022864164064627No Hit

[OK]Adapter Content

Adapter graph

[WARN]Kmer Content

Kmer graph

SequenceCountPValueObs/Exp MaxMax Obs/Exp Position
AGGACGT200.006903019352.59110652
GACTATA200.00755430251.40562430
GGTATCA6900.047.5673141
TCGTATG1450.045.2870840
CGTATGC1500.043.80556541
GCAATCT1400.041.7034534
AATCTCG1450.040.339236
ATACACT350.001409778139.0669674
ATCTCGT1550.037.76375237
ATGCCGT1750.037.65015444
GTATGCC1750.037.59279342
ACGAGAC1750.037.13125622
TATACAC1850.036.955243
CCGTCTT1800.036.73709547
CGAGACA1900.036.0026823
CTTATAC1900.035.988431
GCCGTCT1900.034.74754746
AATGGTA2400.034.20255370
CATGTAA400.002714339934.18630210
GTATCAA15850.034.0811581
Produced by FastQC (version 0.11.5)