FastQCFastQC Report
Sat 23 Apr 2022
EGAF00004684403

Summary

[OK]Basic Statistics

MeasureValue
FilenameEGAF00004684403
File typeConventional base calls
EncodingSanger / Illumina 1.9
Total Sequences631556
Sequences flagged as poor quality0
Sequence length20-76
%GC55

[OK]Per base sequence quality

Per base quality graph

[OK]Per tile sequence quality

Per base quality graph

[OK]Per sequence quality scores

Per Sequence quality graph

[FAIL]Per base sequence content

Per base sequence content

[FAIL]Per sequence GC content

Per sequence GC content graph

[OK]Per base N content

N content graph

[WARN]Sequence Length Distribution

Sequence length distribution

[FAIL]Sequence Duplication Levels

Duplication level graph

[WARN]Overrepresented sequences

SequenceCountPercentagePossible Source
CTCCTGGGAGTTACCCGATTGGAGGGCGTTATCCACCTTCCACTGTACTTTGGCCTCTCTGGGATAGAAGTTATT20500.3245951269562794No Hit
GGGGGGGGGGGGGGGGGGGGGGGGGGGGGGGGGGGGGGGGGGGGGGGGGG16810.2661680041041491No Hit
CTCTAACACTCTCCCCTGTTGAAGCTCTTTGTGACGGGCGAGCTCAGGCC13800.2185079391217881No Hit
GTTAGGACCCAGAGGGAACCATGGAAACCCCAGCGCAGCTTCTCTTCCTCCTGCTACTCTGGCTCCCAGATACCA13640.21597451374066592No Hit
GGCGTAGACTTTGTGTTTCTCGTAGTCTGCTTTGCTCAGCGTCAGGGTGC12250.19396538074216696No Hit
ACCCTGGCCAGGCTCCCAGGCTCCTCATCTATGGTGCATCCAGCAGGGCC11410.1806648974912755No Hit
CTCCCAGGAGAGTGTCACAGAGCAGGACAGCAAGGACAGCACCTACAGCC11230.17781479393751307No Hit
GTCCTGCTCTGTGACACTCTCCTGGGAGTTACCCGATTGGAGGGCGTTATCCACCTTCCACTGTACTTTGGCCT11190.1771814375922325No Hit
CAACAGAGGCAGTTCCAGATTTCAACTGCTCATCAGATGGCGGGAAGATG10880.1722729259163083No Hit
GAAATACACAGCCGAGTCCGCGGCGTTCACAGAGCTCAACTTCAGGGAGA10740.17005617870782638No Hit
GTCTCCAGGCACCCTGTCTTTGTCTCCAGGGGAAAGAGCCACCCTCTCCTGCAGGGCCAGTCAGAGTGTTAGCAG9940.1573890518022155No Hit
GTTTTACTGTCAGCAATATGGTAGTTCACCCTACACTTTTGGCCAGGGGA9590.1518471837810107No Hit
CTCCTGCAGGGCCAGTCAGAGTGTTAGCAGCAGCTACTTAGCCTGGTACC9550.15121382743573017No Hit
CATCTATGGTGCATCCAGCAGGGCCACTGGCATCCCAGACAGGTTCAGTGGCAGTGGCTCTGGGACAGACTTCA9540.15105548834941002No Hit
GTTCAAGGACCACCTGAGCGTGACCTCTGTGGCTGCTACAGCGTGTCCAG9410.14899708022724825No Hit
GCGTCAGGGTGCTGCTGAGGCTGTAGGTGCTGTCCTTGCTGTCCTGCTCT8850.14013009139332064No Hit
GGCTGGAACTGAGGAGCAGGTGGGGGCACTTCTCCCTCTAACACTCTCCC8830.13981341322068036No Hit
ATATGGTAGTTCACCCTACACTTTTGGCCAGGGGACCAAGCTGGAGATCAAACGAACTGTGGCTGCACCAT8770.13886337870275953No Hit
GTCACTGCACCGACCGGCCCTCGAGGACCTGCTCTTAGGTTCAGAAGCGAACCTCACGTGCACACTGACCGGC8690.13759666601219844No Hit
CTCCAGGCACCCTGTCTTTGTCTCCAGGGGAAAGAGCCACCCTCTCCTGC8690.13759666601219844No Hit
CTCTGGGATAGAAGTTATTCAGCAGGCACACAACAGAGGCAGTTCCAGAT8620.13648829240795748No Hit
GTGTACAGGTCCCCGGAGGCATCCTGGCTGGGTGGGAAGTTTCTGGCGGT8530.13506324063107628No Hit
GTCTTTGTCTCCAGGGGAAAGAGCCACCCTCTCCTGCAGGGCCAGTCAGA8500.13458822337211587No Hit
CCACTGTACTTTGGCCTCTCTGGGATAGAAGTTATTCAGCAGGCACACAACAGAGGCAGTTCCAGATTTCAACTG8360.13237147616363396No Hit
GTACAGTGGAAGGTGGATAACGCCCTCCAATCGGGTAACTCCCAGGAGAG8330.13189645890467352No Hit
GTGTGACCTGGAGCGAAAGCGGACAGGGCGTGACCGCCAGAAACTTCCCACCCAGCCAGGATGCCTCCGGGGAC8310.13157978073203325No Hit
GTGCAGTGACAGTCGGGGGTGGCAGCATGAGGGAGATGGGGTAGGTGGAG8200.12983805078251176No Hit
GTGTATTTCTGTGCGAGAGGCCCGTTTTTATATGGTTCGGGGAGACCTCCGTTTGACTCCTGGGGCCAGGGAACC8180.12952137260987148No Hit
GACTTCACTCTCACCATCAGCAGACTGGAGCCTGAAGACTTTGCTGTGTTTTACTGTCAGCAATATGGTAGTTCA7840.12413784367498684No Hit
CAATAGGGGTAGGTCCCCTGTGGAAAAAGGGTCAGAGGCCAAAGGATGGGAGGGGGTCAGGCTGGAACTGAGGAG7700.12192109646650495No Hit
GTCTTCATCTTCCCGCCATCTGATGAGCAGTTGAAATCTGGAACTGCCTCTGTTGTGTGCCTGCTGAATAACTT7650.12112940103490427No Hit
GTCTACGCCTGCGAAGTCACCCATCAGGGCCTGAGCTCGCCCGTCACAAA7570.11986268834434319No Hit
GGCTGTAGGTGCTGTCCTTGCTGTCCTGCTCTGTGACACTCTCCTGGGAGTTACCCGATTGGAGGGCGTTATCCA7550.11954601017170291No Hit
GTCACATCCTGGCTGGGATTCGTGTAGTGCTTCACGTGGCATGTCACGGA7510.11891265382642235No Hit
GTCATGGACCTCCTGCACAAGAACATGAAACACCTGTGGTTCTTCCTCCTCCTGGTGGCAGCTCCCAGATGGGTC7500.11875431474010223No Hit
GAGCAGGACAGCAAGGACAGCACCTACAGCCTCAGCAGCACCCTGACGCT7380.11685424570426059No Hit
GTGGCTGCACCATCTGTCTTCATCTTCCCGCCATCTGATGAGCAGTTGAAATCTGGAACTGCCTCTGTTGTGTGC7250.11479583758209883No Hit
GTGTAGGGTGAACTACCATATTGCTGACAGTAAAACACAGCAAAGTCTTC7170.11352912489153773No Hit
GATGAAGACAGATGGTGCAGCCACAGTTCGTTTGATCTCCAGCTTGGTCC7170.11352912489153773No Hit
CGTGCACACTGACCGGCCTGAGAGATGCCTCAGGTGTCACCTTCACCTGGACGCCCTCAAGTGGGAAGAGCG7120.11273742945993705No Hit
CCATAGATGAGGAGCCTGGGAGCCTGGCCAGGGTTGTGCTGGTACCAGGCTAAGTAGCTGCTGCTAACACTCTGA7020.11115403859673569No Hit
GTGCAGCCACAGTTCGTTTGATCTCCAGCTTGGTCCCCTGGCCAAAAGTG7000.11083736042409541No Hit
GGATGGGAGGGGGTCAGGCTGGAACTGAGGAGCAGGTGGGGGCACTTCTCCCTCTAACACTCTCCCCTGTTGAAG6950.11004566499249473No Hit
CTACTGGACCTGGATCCGCCAGTCCCCAGGGAAGGGGCCGGAGTGGATTGGGGAAATCAGTCACAGTGGAATCAC6940.1098873259061746No Hit
GTCCTTGCTGTCCTGCTCTGTGACACTCTCCTGGGAGTTACCCGATTGGAGGGCGTTATCCACCTTCCACTGTA6820.10798725687033296No Hit
GTCCAAGACCCCGCTAACCGCCACCCTCTCAAAATCCGGAAACACATTCC6810.10782891778401281No Hit
GCTCAGTTAGGACCCAGAGGGAACCATGGAAACCCCAGCGCAGCTTCTCT6780.1073539005250524No Hit
GGGTTGTGCTGGTACCAGGCTAAGTAGCTGCTGCTAACACTCTGACTGGCCCTGCAGGAGAGGGTGGCTCTTTCC6770.10719556143873228No Hit
GCACAACCCTGGCCAGGCTCCCAGGCTCCTCATCTATGGTGCATCCAGCAGGGCCACTGGCATCCCAGACAGGTT6720.1064038660071316No Hit
AGGTCACACTGAGTGGCTCCTGGGGGAAGAAGCCCTGGACCAGGCAGGCG6600.10450379697128996No Hit
GTACTTTGGCCTCTCTGGGATAGAAGTTATTCAGCAGGCACACAACAGAGGCAGTTCCAGATTTCAACTGCTCAT6530.103395423367049No Hit
GCCCCAAGGTCTTCCCGCTGAGCCTCTGCAGCACCCAGCCAGATGGGAAC6480.10260372793544832No Hit
GTCCAGGGCTTCTTCCCCCAGGAGCCACTCAGTGTGACCTGGAGCGAAAGCGGACAGGGCGTGACCGCCAGAAA6480.10260372793544832No Hit
CAGTAGACCCGTCCAAGGACCAGTTCTCCCTGAAGTTGAGCTCTGTGAACGCCGCGGACTCGGCTGTGTATTT6460.10228704976280804No Hit
TCCTTGGGGCTGAAGCCACGTGCCAGGCACGTCAGCGTCACCAGCTCGTT6450.1021287106764879No Hit
CAGTAAAACACAGCAAAGTCTTCAGGCTCCAGTCTGCTGATGGTGAGAGTGAAGTCTGTCCCAGAGCCACTGCCA6320.10007030255432614No Hit

[OK]Adapter Content

Adapter graph

[WARN]Kmer Content

Kmer graph

SequenceCountPValueObs/Exp MaxMax Obs/Exp Position
ATACTAC200.007692733351.1700553
GGTATCA5150.043.735421
GTATCAA13850.037.9461331
TAGGCAA1308.402203E-735.1432270
AAAACCG951.906585E-434.35051770
TATCAAC15700.033.461522
TCAACGC15950.032.937054
ATCAACG16100.032.8420683
GCGCTTT553.4327852E-431.08105526
AAGGCGC553.4439802E-431.063823
ACGCAGA17000.030.9075957
CGCAGAG17250.030.6574488
GCAGAGT17600.030.2416429
AGAGTAC17550.029.93898211
GAGTTTA2450.027.8586791
CAACGCA19150.027.4332075
AACGCAG19350.026.9776366
GAGTACG15250.026.40009912
AGTACGG15250.026.39800813
AGTTTAT2600.026.2410552
Produced by FastQC (version 0.11.5)