FastQCFastQC Report
Sat 23 Apr 2022
EGAF00004684404

Summary

[OK]Basic Statistics

MeasureValue
FilenameEGAF00004684404
File typeConventional base calls
EncodingSanger / Illumina 1.9
Total Sequences722315
Sequences flagged as poor quality0
Sequence length20-76
%GC58

[OK]Per base sequence quality

Per base quality graph

[OK]Per tile sequence quality

Per base quality graph

[OK]Per sequence quality scores

Per Sequence quality graph

[FAIL]Per base sequence content

Per base sequence content

[FAIL]Per sequence GC content

Per sequence GC content graph

[OK]Per base N content

N content graph

[WARN]Sequence Length Distribution

Sequence length distribution

[FAIL]Sequence Duplication Levels

Duplication level graph

[WARN]Overrepresented sequences

SequenceCountPercentagePossible Source
GGGTTAGAGTTGGGAACCTATGAACATTCTGTAGGGGCCACTGTCTTCTCCACGGTGCTCCCTTCATGCGTGAC24280.33614143413884523No Hit
CCTCAGAGGAGGGCGGGAACAGAGTGACAGTGGGGTTGGCCTTGGGCTGA22760.31509798356672647No Hit
CCTCTGAGGAGCTCCAAGCCAACAAGGCCACACTAGTGTGTCTGATCAGT22080.30568380831077857No Hit
GTAATAAACGGCCGTGTCGTCGACTTTCAGGCTGTTCATTTGCAGGTACA21800.30180738320538825No Hit
CACTAGTGTGGCCTTGTTGGCTTGGAGCTCCTCAGAGGAGGGCGGGAACAGAGTGACAGTGGGGTTGGCCTTGGG20770.28754767656770247No Hit
GCCCAAGGCCAACCCCACTGTCACTCTGTTCCCGCCCTCCTCTGAGGAGC19190.26567356347300003No Hit
GACCTAGGACGGTGACCTTGGTCCCAGTTCCGAAGACATGAATGCCAGTGCCCCAGGTCTGACAGTAATAGTCAG19170.2653966759654721No Hit
GTTTGGAGGGTTTGGTGGTCTCCACTCCCGCCTTGACGGGGCTGCCATCTGCCTTCCAGGCCACTGTCACAGCTC18810.2604127008299703No Hit
GCTCTGGGGAGTCTGCACCATGGCTTGGACCCCACTCCTCTTCCTCACCCTCCTCCTCCACTACACAGGGTCTCT18630.2579207132622194No Hit
GTCCTAGGTCAGCCCAAGGCCAACCCCACTGTCACTCTGTTCCCGCCCTC18380.2544596194181209No Hit
CCACTACACAGGGTCTCTCTCCCAGCTTGTGCTGACTCAATCGCCCTCTGCCTCTGCCTCCCTGGGAGCCTCGGT17270.2390923627503236No Hit
GGACTGAACAGAGAGAACTCACCATGGAGTTTGGGCTGAGCTGGCTTTTT17000.23535438139869724No Hit
GTGCAGGGAGAAGGGCTGGATGACTTGGGATGGGGAGAGAGACCCCTCCCCTGGGATCCTGCAGCTCCAGGCTCC16830.23300083758471027No Hit
CTTATACACATCTCCGAGCCCACGAGACTCGACGTCATCTCGTATGCCGT16780.23230861881589057No Hit
GACTATTACTGTCAGACCTGGGGCACTGGCATTCATGTCTTCGGAACTGGGACCAAGGTCACCGTCCTAGGTCAG16300.22566331863522146No Hit
GTAATAGTCAGCCTCATCGTCAGACTGGAGGCTGGAGATGGTGAGGTAGC16260.22510954362016572No Hit
GCCTTGGGCTGACCTAGGACGGTGACCTTGGTCCCAGTTCCGAAGACATG15190.21029606196742417No Hit
GTCACATCCTGGCTGGGATTCGTGTAGTGCTTCACGTGGCATGTCACGGA15090.20891162442978478No Hit
GTGCAGTGACAGTCGGGGGTGGCAGCATGAGGGAGATGGGGTAGGTGGAG14510.2008818867114763No Hit
GTCTTCTCCACGGTGCTCCCTTCATGCGTGACCTGGCAGCTGTAGCTTCT14510.2008818867114763No Hit
ACCTAGGACGGTGACCTTGGTCCCAGTTCCGAAGACATGAATGCCAGTGCCCCAGGTCTGACAGTAATAGTCAGC14450.20005122418889268No Hit
GCTTAACAGTGATGGCAGCCACAGCAAGGGGGACGGGATCCCTGATCGCT14440.19991278043512872No Hit
ACACTAGTGTGTCTGATCAGTGACTTCTACCCGGGAGCTGTGACAGTGGC14370.19894367415878114No Hit
CTCCTGTGCAGCCTCTGGATTCACCTTTAGCAGCTATGCCATGAGCTGGGTCCGCCAGGCTCCAGGGAAGGGG14080.1949288052996269No Hit
GCCCCAGAGCTGGAGCCTGAGAAGCGATCAGGGATCCCGTCCCCCTTGCT14050.19451347403833508No Hit
GTGTACAGGTCCCCGGAGGCATCCTGGCTGGGTGGGAAGTTTCTGGCGGT13980.1935443677619875No Hit
GAGTGACAGTGGGGTTGGCCTTGGGCTGACCTAGGACGGTGACCTTGGTC13800.1910523801942366No Hit
CCTATGAACATTCTGTAGGGGCCACTGTCTTCTCCACGGTGCTCCCTTCATGCGTGACCTGGCAGCTGTAGCTT13470.1864837363200266No Hit
CTGTTAAGCTTCATCAAGTACCGAGGGCCCTTCTCTGGCTGCTGCTGATGCCATGCGATGGCGTAGCTGTTGTGC13330.18454552376733144No Hit
GTCGTATAGCCACTGCTATACCCGGTCCTCGCACAGTAATAAACGGCCGT13200.1827457549684002No Hit
CCTGCACTCTGAGCGGTGGGCACAACAGCTACGCCATCGCATGGCATCAG12980.17969999238559353No Hit
GTACTTGATGAAGCTTAACAGTGATGGCAGCCACAGCAAGGGGGACGGGATCCCTGATCGCTTCTCAGGCTCCAG12610.17457757349632777No Hit
GAGAAGGGCTGGATGACTTGGGATGGGGAGAGAGACCCCTCCCCTGGGAT12550.17374691097374415No Hit
AGGTCACACTGAGTGGCTCCTGGGGGAAGAAGCCCTGGACCAGGCAGGCG12490.17291624845116052No Hit
GTTTATTACTGTGCGAGGACCGGGTATAGCAGTGGCTATACGACTGACTGGGGCCAGGGAACCCTGGTCACCGT12470.1726393609436326No Hit
GCTATACCCGGTCCTCGCACAGTAATAAACGGCCGTGTCGTCGACTTTCA12430.17208558592857687No Hit
GTGCTCCCTTCATGCGTGACCTGGCAGCTGTAGCTTCTGTGGGACTTCCACTGCTCGGGCGTCAGGCTCAGGTAG12080.16724005454683896No Hit
CTATTACTGTCAGACCTGGGGCACTGGCATTCATGTCTTCGGAACTGGGACCAAGGTCACCGTCCTAGGTCAGCC11990.16599406076296352No Hit
TCCTAGGTCAGCCCAAGGCCAACCCCACTGTCACTCTGTTCCCGCCCTCCTCTGAGGAGCTCCAAGCCAACAAGG11610.1607331981199338No Hit
CACCTGCACTCTGAGCGGTGGGCACAACAGCTACGCCATCGCATGGCATC11550.15990253559735018No Hit
GTCACTGCACCGACCGGCCCTCGAGGACCTGCTCTTAGGTTCAGAAGCGAACCTCACGTGCACACTGACCGGC11220.1553338917231402No Hit
GGGTCTCTCTCCCAGCTTGTGCTGACTCAATCGCCCTCTGCCTCTGCCTCCCTGGGAGCCTCGGTCAAGCTCAC11080.15339567917044503No Hit
GTTTGGTGGTCTCCACTCCCGCCTTGACGGGGCTGCCATCTGCCTTCCAG11030.15270346040162533No Hit
CTTCATGCGTGACCTGGCAGCTGTAGCTTCTGTGGGACTTCCACTGCTCGGGCGTCAGGCTCAGGTAGCTGCTGG10930.15131902286398594No Hit
ACACAGGGTCTCTCTCCCAGCTTGTGCTGACTCAATCGCCCTCTGCCTCTGCCTCCCTGGGAGCCTCGGTCAAG10880.15062680409516624No Hit
GCTCTGGGGCTGAGCGCTACCTCACCATCTCCAGCCTCCAGTCTGACGATGAGGCTGACTATTACTGTCAGAC10850.15021147283387443No Hit
GTACGGGAGAGCTCTGGGGAGTCTGCACCATGGCTTGGACCCCACTCCTCTTCCTCACCCTCCTCCTCCACTACA10770.1491039228037629No Hit
GTGTGACCTGGAGCGAAAGCGGACAGGGCGTGACCGCCAGAAACTTCCCACCCAGCCAGGATGCCTCCGGGGAC10430.14439683517578897No Hit
CAGTAATAGTCAGCCTCATCGTCAGACTGGAGGCTGGAGATGGTGAGGTAGCGCTCAGCCCCAGAGCTGGAGC10320.14287395388438562No Hit
GGCTTGGACCCCACTCCTCTTCCTCACCCTCCTCCTCCACTACACAGGGT10290.1424586226230938No Hit
CTCCCAGCTTGTGCTGACTCAATCGCCCTCTGCCTCTGCCTCCCTGGGAGCCTCGGTCAAGCTCACCTGCACT10260.142043291361802No Hit
GTCCAGGGCTTCTTCCCCCAGGAGCCACTCAGTGTGACCTGGAGCGAAAGCGGACAGGGCGTGACCGCCAGAAA10190.1410741850854544No Hit
GTATGCTGGTCACAGCGAAGGTGGTGGTGCCCTGGCTGGGCTCCTGCCGG10050.13913597253275925No Hit
CAGCAAGGGGGACGGGATCCCTGATCGCTTCTCAGGCTCCAGCTCTGGGGCTGAGCGCTACCTCACCATCTCCAG9920.13733620373382804No Hit
GCCCCAAGGTCTTCCCGCTGAGCCTCTGCAGCACCCAGCCAGATGGGAAC9780.13539799118113288No Hit
GTTCAAGGACCACCTGAGCGTGACCTCTGTGGCTGCTACAGCGTGTCCAG9760.13512110367360503No Hit
GGCTCAGGTAGCTGCTGGCCGCGTACTTGTTGTTGCTCTGTTTGGAGGGTTTGGTGGTCTCCACTCCCGCCTTGA9740.1348442161660771No Hit
CCCTTCATGCGTGACCTGGCAGCTGTAGCTTCTGTGGGACTTCCACTGCTCGGGCGTCAGGCTCAGGTAGCTG9720.13456732865854926No Hit
GGGTATAGCAGTGGCTATACGACTGACTGGGGCCAGGGAACCCTGGTCACCGTCTCCTCAGCATCCCCGACCAGC9540.13207534109079835No Hit
AGTGTGGCCTTGTTGGCTTGGAGCTCCTCAGAGGAGGGCGGGAACAGAGTGACAGTGGGGTTGGCCTTGGGCTGA9350.12944490976928347No Hit
CTGCTATACCCGGTCCTCGCACAGTAATAAACGGCCGTGTCGTCGACTTT9330.1291680222617556No Hit
GTGCCTAGCCGGCAAGTCCGTGACATGCCACGTGAAGCACTACACGAATCCCAGCCAGGATGTGACTGTGCCCTG9140.12653759094024075No Hit
CTGTCACTCTGTTCCCGCCCTCCTCTGAGGAGCTCCAAGCCAACAAGGCC9070.12556848466389317No Hit
GAGTGGCTCCTGGGGGAAGAAGCCCTGGACCAGGCAGGCGATGACCACGT9030.12501470964883743No Hit
GCCCTGGACCAGGCAGGCGATGACCACGTTCCCATCTGGCTGGGTGCTGC8800.1218305033122668No Hit
GTTCCCGCCCTCCTCTGAGGAGCTCCAAGCCAACAAGGCCACACTAGTGT8780.12155361580473893No Hit
GAACAGAGAGAACTCACCATGGAGTTTGGGCTGAGCTGGCTTTTTCTTGT8720.1207229532821553No Hit
GCTCAGTAGCAGGTGCCGTCCACCTCCGCCATGACAACAGACACATTGAC8710.12058450952839135No Hit
GAGTACGGGAGAGCTCTGGGGAGTCTGCACCATGGCTTGGACCCCACTCCTCTTCCTCACCCTCCTCCTCCACTA8660.11989229075957165No Hit
GTCTTGGACTCGGGGTAGGCAGCAGTGCAAGTGAAGGTCTTCCCATGGTT8620.1193385157445159No Hit
GTGTGCACGTGAGGTTCGCTTCTGAACCTAAGAGCAGGTCCTCGAGGGCC8580.11878474072946013No Hit
CCCCTGGGATCCTGCAGCTCCAGGCTCCCGTGGGTGGGGTTAGAGTTGGG8570.11864629697569619No Hit
GCTAGGCACTGTGTGGCCGGCAGGGTCAGCTGGCTGCTCGTGGTGTACAG8510.11781563445311258No Hit
CCTTGGGGCTGGTCGGGGATGCTGAGGAGACGGTGACCAGGGTTCCCTGGCCCCAGTCAGTCGTATAGCCACTG8500.11767719069934862No Hit
CTCCTCTTCCTCACCCTCCTCCTCCACTACACAGGGTCTCTCTCCCAGCTTGTGCTGACTCAATCGCCCTCTGC8460.11712341568429285No Hit
GTATAGCAGTGGCTATACGACTGACTGGGGCCAGGGAACCCTGGTCACCGTCTCCTCAGCATCCCCGACCAGCCC8410.11643119691547316No Hit
GTACAGGTCCCCGGAGGCATCCTGGCTGGGTGGGAAGTTTCTGGCGGTCACGCCCTGTCCGCTTTCGCTCCAGGT8350.11560053439288952No Hit
GAGTTTGGGCTGAGCTGGCTTTTTCTTGTGGCTATTTTAAAAGGTGTCCAGTGTGAGGTGCAGCTATTGGAGT8300.11490831562406983No Hit
GGATGTGACTGTGCCCTGCCCAGTTCCCTCAACTCCACCTACCCCATCTCCCTCAACTCCACCTACCCCATCTCC8240.11407765310148618No Hit
GATCAGGACTGAACAGAGAGAACTCACCATGGAGTTTGGGCTGAGCTGGC8170.11310854682513863No Hit
GGATATTTATTGGGGTTTCATGAGTGCAGGGAGAAGGGCTGGATGACTTGGGATGGGGAGAGAGACCCCTCCC8150.11283165931761073No Hit
GAGTTTATTCAGGGGTGGGGACAGGCGGGCGGCTCAGTAGCAGGTGCCGT8050.11144722177997134No Hit
ATCAAGTACCGAGGGCCCTTCTCTGGCTGCTGCTGATGCCATGCGATGGC8040.1113087780262074No Hit
TCCTCACCCTCCTCCTCCACTACACAGGGTCTCTCTCCCAGCTTGTGCTGACTCAATCGCCCTCTGCCTCTGCCT8000.11075500301115165No Hit
GTGTGAGGTGCAGCTATTGGAGTCTGGGGGAGGCTTGGTACAGCCGGGGGGGTCCCTGAGACTCTCCTGTGCAGC7940.109924340488568No Hit
CTGTCACAGCTCCCGGGTAGAAGTCACTGATCAGACACACTAGTGTGGCC7940.109924340488568No Hit
CCCGTGGGTGGGGTTAGAGTTGGGAACCTATGAACATTCTGTAGGGGCCA7900.10937056547351223No Hit
GGGCACAACAGCTACGCCATCGCATGGCATCAGCAGCAGCCAGAGAAGGG7900.10937056547351223No Hit
GGGTTCCCTGGCCCCAGTCAGTCGTATAGCCACTGCTATACCCGGTCCTCGCACAGTAATAAACGGCCGTGTCGT7780.10770924042834498No Hit
GGGTAGAAGTCACTGATCAGACACACTAGTGTGGCCTTGTTGGCTTGGAG7720.10687857790576134No Hit
GTGTAGTGCTTCACGTGGCATGTCACGGACTTGCCGGCTAGGCACTGTGT7720.10687857790576134No Hit
CTACCTCACCATCTCCAGCCTCCAGTCTGACGATGAGGCTGACTATTACT7580.10494036535306618No Hit
GTCACGGACTTGCCGGCTAGGCACTGTGTGGCCGGCAGGGTCAGCTGGCT7580.10494036535306618No Hit
GGGATGGGGAGAGAGACCCCTCCCCTGGGATCCTGCAGCTCCAGGCTCCCGTGGGTGGGGTTAGAGTTGGGAAC7580.10494036535306618No Hit
GGGCTGGATGACTTGGGATGGGGAGAGAGACCCCTCCCCTGGGATCCTGC7570.10480192159930224No Hit
CTAGTGTGTCTGATCAGTGACTTCTACCCGGGAGCTGTGACAGTGGCCTG7560.10466347784553831No Hit
CAGTATGCTGGTCACAGCGAAGGTGGTGGTGCCCTGGCTGGGCTCCTGCC7520.10410970283048254No Hit
GGGCTGGAGTGGGTCTCAGCTCTTAGTGGTGCTGCTACTGGCAGATACTA7490.10369437156919073No Hit
CGTGCACACTGACCGGCCTGAGAGATGCCTCAGGTGTCACCTTCACCTGGACGCCCTCAAGTGGGAAGAGCG7450.10314059655413498No Hit
CCCTGGGAGCCTCGGTCAAGCTCACCTGCACTCTGAGCGGTGGGCACAAC7420.10272526529284316No Hit
GTATCAACGCAGAGTACGGGAGCTCTGAGAGAGGAGCCCAGCCCTGGGAT7400.10244837778531526No Hit
GCATGAGGGAGATGGGGTAGGTGGAGTTGAGGGAGATGGGGTAGGTGGAGTTGAGGGAACTGGGCAGGGCACAGT7330.1014792715089677No Hit
CTTCTACCCGGGAGCTGTGACAGTGGCCTGGAAGGCAGATGGCAGCCCCG7320.10134082775520376No Hit
CTATACGACTGACTGGGGCCAGGGAACCCTGGTCACCGTCTCCTCAGCAT7270.10064860898638406No Hit
CTGTAGGGGCCACTGTCTTCTCCACGGTGCTCCCTTCATGCGTGACCTGG7250.10037172147885619No Hit

[OK]Adapter Content

Adapter graph

[FAIL]Kmer Content

Kmer graph

SequenceCountPValueObs/Exp MaxMax Obs/Exp Position
AGGGGGG3150.079.68548670
TAGTACC150.002260155369.7202749
CGTATGC2800.056.7845141
CTCGACG2850.055.22700528
ACTCGAC2850.055.18105727
TCGACGT2800.055.0178929
GTATGCC2900.054.88797842
TCGTATG2900.054.7688440
CGAGACT3050.053.733723
TCTCGTA2700.053.5904338
GCTCGGT200.00675312752.88462456
ATCTCGT2750.052.54631437
GCCGTCT3050.052.41291846
CTTATAC3200.052.3108831
ATGCCGT3050.052.309744
ATCTCCG3150.052.02426510
ACGAGAC3150.052.0206622
TATACAC3300.051.721513
TTATACA3250.051.466882
AATGATC200.007587124651.35003729
Produced by FastQC (version 0.11.5)