FastQCFastQC Report
Wed 27 Apr 2022
EGAF00004684407

Summary

[OK]Basic Statistics

MeasureValue
FilenameEGAF00004684407
File typeConventional base calls
EncodingSanger / Illumina 1.9
Total Sequences876545
Sequences flagged as poor quality0
Sequence length20-76
%GC55

[OK]Per base sequence quality

Per base quality graph

[OK]Per tile sequence quality

Per tile quality graph

[OK]Per sequence quality scores

Per Sequence quality graph

[FAIL]Per base sequence content

Per base sequence content

[WARN]Per sequence GC content

Per sequence GC content graph

[OK]Per base N content

N content graph

[WARN]Sequence Length Distribution

Sequence length distribution

[FAIL]Sequence Duplication Levels

Duplication level graph

[WARN]Overrepresented sequences

SequenceCountPercentagePossible Source
ATATTATACCAGTCCGCTCACTTTCGGCGGAGGGACCAACGTGGAGATCAAACGAACTGTGGCTGCACCAT48510.5534228134322824No Hit
GGGGGGGGGGGGGGGGGGGGGGGGGGGGGGGGGGGGGGGGGGGGGGGGGG34400.3924499027431564No Hit
CTCCTGGGAGTTACCCGATTGGAGGGCGTTATCCACCTTCCACTGTACTTTGGCCTCTCTGGGATAGAAGTTATT29300.3342669229760024No Hit
CTCTAACACTCTCCCCTGTTGAAGCTCTTTGTGACGGGCGAGCTCAGGCC28490.3250260967776897No Hit
GTATAATATTGCAGACAATAATAAACTGCCACATCTTCAGCCTGCAGGCT26210.2990148822935502No Hit
CTCCCAGGAGAGTGTCACAGAGCAGGACAGCAAGGACAGCACCTACAGCC23790.27140648797266537No Hit
GGCGTAGACTTTGTGTTTCTCGTAGTCTGCTTTGCTCAGCGTCAGGGTGC20370.23238966624645627No Hit
GTCTACGCCTGCGAAGTCACCCATCAGGGCCTGAGCTCGCCCGTCACAAA20320.23181924487619002No Hit
GTACAGTGGAAGGTGGATAACGCCCTCCAATCGGGTAACTCCCAGGAGAG19720.22497418843299546No Hit
GGTATAATATTGCAGACAATAATAAACTGCCACATCTTCAGCCTGCAGGCTGCCGATGGTGAGAGTGAAAT19480.22223616585571762No Hit
GGCTGGAACTGAGGAGCAGGTGGGGGCACTTCTCCCTCTAACACTCTCCC18930.21596153078278926No Hit
ACTTAGGAGGCTGTCCTGGTTTCTGCTGGAACCAAGCTAAGTAATTCTTA18700.21333759247956466No Hit
ATTTTATACAACTCCGACAGTAAGAATTACTTAGCTTGGTTCCAGCAGAA17280.19713762556400413No Hit
GTCTTCATCTTCCCGCCATCTGATGAGCAGTTGAAATCTGGAACTGCCTCTGTTGTGTGCCTGCTGAATAACTT16580.1891517263802771No Hit
GGATGGGAGGGGGTCAGGCTGGAACTGAGGAGCAGGTGGGGGCACTTCTCCCTCTAACACTCTCCCCTGTTGAAG16540.18869538928406412No Hit
GTCCTGCTCTGTGACACTCTCCTGGGAGTTACCCGATTGGAGGGCGTTATCCACCTTCCACTGTACTTTGGCCT16500.18823905218785117No Hit
CAACAGAGGCAGTTCCAGATTTCAACTGCTCATCAGATGGCGGGAAGATG16050.18310525985545523No Hit
GAGCAGGACAGCAAGGACAGCACCTACAGCCTCAGCAGCACCCTGACGCT15990.18242075421113577No Hit
GCGTCAGGGTGCTGCTGAGGCTGTAGGTGCTGTCCTTGCTGTCCTGCTCT15130.17260950664255686No Hit
CAATAGGGGTAGGTCCCCTGTGGAAAAAGGGTCAGAGGCCAAAGGATGGGAGGGGGTCAGGCTGGAACTGAGGAG14450.16485177600693632No Hit
CCACTGTACTTTGGCCTCTCTGGGATAGAAGTTATTCAGCAGGCACACAACAGAGGCAGTTCCAGATTTCAACTG13150.1500208203800147No Hit
CCCTGACGCTGAGCAAAGCAGACTACGAGAAACACAAAGTCTACGCCTGCGAAGTCACCCATCAGGGCCTGAGCT12660.14443069095140582No Hit
GGCTGTAGGTGCTGTCCTTGCTGTCCTGCTCTGTGACACTCTCCTGGGAGTTACCCGATTGGAGGGCGTTATCCA12600.14374618530708635No Hit
GTGGCTGCACCATCTGTCTTCATCTTCCCGCCATCTGATGAGCAGTTGAAATCTGGAACTGCCTCTGTTGTGTGC12520.14283351111466042No Hit
CTCCTAAGTTGCTCATTAACTGGGCATCTACCCGGCAATCCGGGGTCCCTGACCGATTCAGTGGCAGCGGGTCTG12360.1410081627298085No Hit
GTGTTGCAGACCCAGGTCTTCATTTCTCTGTTGCTCTGGATCTCTGGTGC12190.13906873007090337No Hit
CCTACAGCCTCAGCAGCACCCTGACGCTGAGCAAAGCAGACTACGAGAAACACAAAGTCTACGCCTGCGAAGTCA11680.133250432094188No Hit
ATAATAAACTGCCACATCTTCAGCCTGCAGGCTGCCGATGGTGAGAGTGAAATCTGTCGCAGACCCGCTGCCA11410.1301701566947504No Hit
GGCCAAAGGATGGGAGGGGGTCAGGCTGGAACTGAGGAGCAGGTGGGGGCACTTCTCCCTCTAACACTCTCCC11400.13005607242069717No Hit
ATCCCAGAGAGGCCAAAGTACAGTGGAAGGTGGATAACGCCCTCCAATCG10780.1229828474293961No Hit
CTCTGGGATAGAAGTTATTCAGCAGGCACACAACAGAGGCAGTTCCAGAT10770.12286876315534284No Hit
GTTTATTATTGTCTGCAATATTATACCAGTCCGCTCACTTTCGGCGGAGGGACCAACGTGGAGATCAAACGAA10710.12218425751102338No Hit
GTTGTGTGCCTGCTGAATAACTTCTATCCCAGAGAGGCCAAAGTACAGTG10690.12195608896291692No Hit
CAATAATAAACTGCCACATCTTCAGCCTGCAGGCTGCCGATGGTGAGAGTGAAATCTGTCGCAGACCCGCTGCCA10580.12070116194833123No Hit
GTATAAAATACTCCGGCTGGACTTGCAGTTGATGGTGGCCCTCTCGCCCAGAGACACAGCCAGGGAGTCTGGAGA10330.11784905509700015No Hit
GTGCAGCCACAGTTCGTTTGATCTCCACGTTGGTCCCTCCGCCGAAAGTG9940.11339976840892367No Hit
GTCTCCAGACTCCCTGGCTGTGTCTCTGGGCGAGAGGGCCACCATCAACTGCAAGTCCAGCCGGAGTATTTTATA9840.11225892566839124No Hit
GTGTCACAGAGCAGGACAGCAAGGACAGCACCTACAGCCTCAGCAGCACC9810.11191667284623152No Hit
GAGTTTATTCAGGGGTGGGGACAGGCGGGTGGCTCAGTAGCAGGTGCCGT9780.11157442002407178No Hit
GTCCCTGACCGATTCAGTGGCAGCGGGTCTGCGACAGATTTCACTCTCAC9770.11146033575001853No Hit
GTTCAAGGACCACCTGAGCGTGACCTCTGTGGCTGCTACAGCGTGTCCAG9700.11066174583164584No Hit
GAGCAAAGCAGACTACGAGAAACACAAAGTCTACGCCTGCGAAGTCACCC9580.10929273454300692No Hit
AACCAGGACAGCCTCCTAAGTTGCTCATTAACTGGGCATCTACCCGGCAA9370.1068969647878888No Hit
GTCCTTGCTGTCCTGCTCTGTGACACTCTCCTGGGAGTTACCCGATTGGAGGGCGTTATCCACCTTCCACTGTA9170.10461527930682396No Hit
GTACTTTGGCCTCTCTGGGATAGAAGTTATTCAGCAGGCACACAACAGAGGCAGTTCCAGATTTCAACTGCTCAT9130.10415894221061098No Hit
CCTCTGTTGTGTGCCTGCTGAATAACTTCTATCCCAGAGAGGCCAAAGTA9130.10415894221061098No Hit
GCTTTGCTCAGCGTCAGGGTGCTGCTGAGGCTGTAGGTGCTGTCCTTGCT9060.10336035229223828No Hit
TCTTCAGCCTGCAGGCTGCCGATGGTGAGAGTGAAATCTGTCGCAGACCCGCTGCCACTGAATCGGTCAGGGACC9040.10313218374413179No Hit
GTGTGACCTGGAGCGAAAGCGGACAGAACGTGACCGCCAGAAACTTCCCACCTAGCCAGGATGCCTCCGGGGAC9010.10278993092197207No Hit
CTTCTATCCCAGAGAGGCCAAAGTACAGTGGAAGGTGGATAACGCCCTCCAATCGGGTAACTCCCAGGAGAGTGT8930.1018772567295461No Hit
CTCCCTGGCTGTGTCTCTGGGCGAGAGGGCCACCATCAACTGCAAGTCCAGCCGGAGTATTTTATACAACTCCGA8930.1018772567295461No Hit
GAATTACTTAGCTTGGTTCCAGCAGAAACCAGGACAGCCTCCTAAGTTGC8920.10176317245549288No Hit
GTCCTGGTTTCTGCTGGAACCAAGCTAAGTAATTCTTACTGTCGGAGTTG8900.10153500390738639No Hit
GTTGAAATCTGGAACTGCCTCTGTTGTGTGCCTGCTGAATAACTTCTATC8870.10119275108522667No Hit
GTCAGGGACCCCGGATTGCCGGGTAGATGCCCAGTTAATGAGCAACTTAGGAGGCTGTCCTGGTTTCTGCTGGAA8860.10107866681117342No Hit
GGCCCTGATGGGTGACTTCGCAGGCGTAGACTTTGTGTTTCTCGTAGTCTGCTTTGCTCAGCGTCAGGGTGCTG8820.10062232971496045No Hit
GTGGAAGGTGGATAACGCCCTCCAATCGGGTAACTCCCAGGAGAGTGTCACAGAGCAGGACAGCAAGGACAGCAC8800.10039416116685396No Hit

[OK]Adapter Content

Adapter graph

Produced by FastQC (version 0.11.9)