FastQCFastQC Report
Sat 23 Apr 2022
EGAF00004684408

Summary

[OK]Basic Statistics

MeasureValue
FilenameEGAF00004684408
File typeConventional base calls
EncodingSanger / Illumina 1.9
Total Sequences632037
Sequences flagged as poor quality0
Sequence length20-76
%GC53

[OK]Per base sequence quality

Per base quality graph

[OK]Per tile sequence quality

Per base quality graph

[OK]Per sequence quality scores

Per Sequence quality graph

[FAIL]Per base sequence content

Per base sequence content

[FAIL]Per sequence GC content

Per sequence GC content graph

[OK]Per base N content

N content graph

[WARN]Sequence Length Distribution

Sequence length distribution

[FAIL]Sequence Duplication Levels

Duplication level graph

[WARN]Overrepresented sequences

SequenceCountPercentagePossible Source
GGGTATATTGAGTGCAGGGAGAAGGGCTTGATGCCTTGGGGTGGGACGAGAGACCCCTCCCCTGGGATCCTGCAG22290.35266922664337685No Hit
GCCCAAGGCTGCCCCCTCGGTCACTCTGTTCCCGCCCTCCTCTGAGGAGC17470.27640786852668436No Hit
CTTATACACATCTCCGAGCCCACGAGACATCTCAGGATCTCGTATGCCGT14480.22910051152068628No Hit
GGTATATTGAGTGCAGGGAGAAGGGCTTGATGCCTTGGGGTGGGACGAGAGACCCCTCCCCTGGGATCCTGCAG14380.22751832566764288No Hit
CCTCTGAGGAGCTTCTAGCCAACAAGGCCACACTGGTGTGTCTCATAAGT11420.18068562441755784No Hit
GTGCAGGGAGAAGGGCTTGATGCCTTGGGGTGGGACGAGAGACCCCTCCCCTGGGATCCTGCAGCTCTAGTCTCC10810.171034290713993No Hit
GAGAAGGGCTTGATGCCTTGGGGTGGGACGAGAGACCCCTCCCCTGGGAT10090.15964255257208043No Hit
GTCTTCTCCACGGTGCTCCCTTCATGCGTGACCTGGCAGCTGTAGCTTCT9810.15521243218355887No Hit
GACCTAGGACGGTCAGTCTGGTCCCTCCTCCGAAGACCAAAGTGCGGGAG9540.15094053038034166No Hit
GCCTTGGGCTGACCTAGGACGGTCAGTCTGGTCCCTCCTCCGAAGACCAAAGTGCGGGAGGTGGAGTCATAGGA9390.14856725160077652No Hit
GTGCTCCCTTCATGCGTGACCTGGCAGCTGTAGCTTCTGTGGGACTTCCACTGCTCAGGCGTCAGGCTCAGATAG9220.14587753565060274No Hit
TCCTAGGTCAGCCCAAGGCTGCCCCCTCGGTCACTCTGTTCCCGCCCTCCTCTGAGGAGCTTCTAGCCAACAAGG9200.14556109847999404No Hit
GAGTTTATTCAGGGGTGGGGACAGGCGGGTGGCTCAGTAGCAGGTGCCGT9160.14492822413877668No Hit
CTCTGTTCCCGCCCTCCTCTGAGGAGCTTCTAGCCAACAAGGCCACACTGGTGTGTCTCATAAGTGACTTCTACC8980.14208028960329852No Hit
GCCTTGGGGTGGGACGAGAGACCCCTCCCCTGGGATCCTGCAGCTCTAGT8480.13416936033808147No Hit
CCTATGAAGATTCTGTAGGGGCCACTGTCTTCTCCACGGTGCTCCCTTCATGCGTGACCTGGCAGCTGTAGCTT8460.1338529231674728No Hit
CTTCATGCGTGACCTGGCAGCTGTAGCTTCTGTGGGACTTCCACTGCTCAGGCGTCAGGCTCAGATAGCTGCTGG8040.12720774258469045No Hit
CCCTTCATGCGTGACCTGGCAGCTGTAGCTTCTGTGGGACTTCCACTGCTCAGGCGTCAGGCTCAGATAGCTG7760.12277762219616889No Hit
GCTCAGGCGTCAGGCTCAGATAGCTGCTGGCCGCGTACTTGTTGTTGCTTTGTTTGGAGGCTGTGGTGGTCTCCA7500.11866393897825601No Hit
TATTTATTGAGGGTATATTGAGTGCAGGGAGAAGGGCTTGATGCCTTGGG7390.11692353453990827No Hit
ATTCAGGGGTGGGGACAGGCGGGTGGCTCAGTAGCAGGTGCCGTCCACCT7370.11660709736929957No Hit
GTTCCCGCCCTCCTCTGAGGAGCTTCTAGCCAACAAGGCCACACTGGTGT7080.11201875839547369No Hit
GTCCTAGGTCAGCCCAAGGCTGCCCCCTCGGTCACTCTGTTCCCGCCCTC6920.10948726103060423No Hit
ACACTGGTGTGTCTCATAAGTGACTTCTACCCGGGAGCCGTGACAGTGGC6910.10932904244529988No Hit
GGACCAGACTGACCGTCCTAGGTCAGCCCAAGGCTGCCCCCTCGGTCACTCTGTTCCCGCCCTCCTCTGAGGAG6740.10663932649512607No Hit
GGCTCAGATAGCTGCTGGCCGCGTACTTGTTGTTGCTTTGTTTGGAGGCTGTGGTGGTCTCCACTCCCGCCTTGA6610.10458248488616963No Hit
GCTCAGTAGCAGGTGCCGTCCACCTCCGCCATGACAACAGACACATTGAC6600.10442426630086529No Hit
GCTCTAGTCTCCCGTGGTGGGGGTGAGGGTTGAGAACCTATGAAGATTCT6590.10426604771556094No Hit
CCCCTGGGATCCTGCAGCTCTAGTCTCCCGTGGTGGGGGTGAGGGTTGAG6480.1025256432772132No Hit

[OK]Adapter Content

Adapter graph

[FAIL]Kmer Content

Kmer graph

SequenceCountPValueObs/Exp MaxMax Obs/Exp Position
AGGGGGG3100.050.37086570
GTATCAA24500.037.5398641
CGTATGC2550.036.59618841
CCCACGA2800.036.21439419
TCGTATG2650.035.15263440
CGAGACA2900.034.9683923
CACGAGA2900.034.96562621
TCTCGTA2400.034.3601338
ACGAGAC3050.034.3569222
TATCAAC28200.032.9610982
CGACAGA951.7667098E-631.9604669
ATCTCCG3250.031.19762610
ATCAACG29800.031.1913683
GTATGCC3000.031.17991642
GTGGTAT3050.031.0418821
TCAACGC30200.030.890164
AACGCAG30500.030.6971446
CAACGCA30550.030.5362635
ATCTCGT2700.030.49328437
TCGTGCA450.005110317430.04215810
Produced by FastQC (version 0.11.5)