FastQCFastQC Report
Sat 23 Apr 2022
EGAF00004684409

Summary

[OK]Basic Statistics

MeasureValue
FilenameEGAF00004684409
File typeConventional base calls
EncodingSanger / Illumina 1.9
Total Sequences632037
Sequences flagged as poor quality0
Sequence length20-76
%GC53

[OK]Per base sequence quality

Per base quality graph

[OK]Per tile sequence quality

Per base quality graph

[OK]Per sequence quality scores

Per Sequence quality graph

[FAIL]Per base sequence content

Per base sequence content

[FAIL]Per sequence GC content

Per sequence GC content graph

[OK]Per base N content

N content graph

[WARN]Sequence Length Distribution

Sequence length distribution

[FAIL]Sequence Duplication Levels

Duplication level graph

[WARN]Overrepresented sequences

SequenceCountPercentagePossible Source
GGGGGGGGGGGGGGGGGGGGGGGGGGGGGGGGGGGGGGGGGGGGGGGGGG29420.46547907796537225No Hit
GGGTATATTGAGTGCAGGGAGAAGGGCTTGATGCCTTGGGGTGGGACGAGAGACCCCTCCCCTGGGATCCTGCAG21060.3332083406509429No Hit
GCCCAAGGCTGCCCCCTCGGTCACTCTGTTCCCGCCCTCCTCTGAGGAGC16860.26675653482311956No Hit
GGTATATTGAGTGCAGGGAGAAGGGCTTGATGCCTTGGGGTGGGACGAGAGACCCCTCCCCTGGGATCCTGCAG14390.22767654425294723No Hit
CCTCTGAGGAGCTTCTAGCCAACAAGGCCACACTGGTGTGTCTCATAAGT10820.17119250929929736No Hit
GTCCTGGGCCCAGCCTGGCCTGACGCAGCCGCCCTCAGTGTCTGCGGCCCCAGGGCAGAGGGTCGTCATCGCCTG10260.16233226852225424No Hit
GTGCTCCCTTCATGCGTGACCTGGCAGCTGTAGCTTCTGTGGGACTTCCACTGCTCAGGCGTCAGGCTCAGATAG9040.14302960111512458No Hit
GTCTTCTCCACGGTGCTCCCTTCATGCGTGACCTGGCAGCTGTAGCTTCT8920.1411309780914725No Hit
TCCTAGGTCAGCCCAAGGCTGCCCCCTCGGTCACTCTGTTCCCGCCCTCCTCTGAGGAGCTTCTAGCCAACAAGG8620.13638442053234223No Hit
GTGCAGGGAGAAGGGCTTGATGCCTTGGGGTGGGACGAGAGACCCCTCCCCTGGGATCCTGCAGCTCTAGTCTCC8540.13511867184990753No Hit
GAGAAGGGCTTGATGCCTTGGGGTGGGACGAGAGACCCCTCCCCTGGGAT8190.1295810213642556No Hit
ATCTGAGCCTGACGCCTGAGCAGTGGAAGTCCCACAGAAGCTACAGCTGC7840.12404337087860363No Hit
GCCTTGGGGTGGGACGAGAGACCCCTCCCCTGGGATCCTGCAGCTCTAGT7810.1235687151226906No Hit
CCTATGAAGATTCTGTAGGGGCCACTGTCTTCTCCACGGTGCTCCCTTCATGCGTGACCTGGCAGCTGTAGCTT7660.12119543634312548No Hit
CTCTGTTCCCGCCCTCCTCTGAGGAGCTTCTAGCCAACAAGGCCACACTGGTGTGTCTCATAAGTGACTTCTACC7600.12024612483129943No Hit
CTTCATGCGTGACCTGGCAGCTGTAGCTTCTGTGGGACTTCCACTGCTCAGGCGTCAGGCTCAGATAGCTGCTGG7500.11866393897825601No Hit
GCCTTGGGCTGACCTAGGACGGTCAGTCTGGTCCCTCCTCCGAAGACCAAAGTGCGGGAGGTGGAGTCATAGGA7500.11866393897825601No Hit
GCTCAGGCGTCAGGCTCAGATAGCTGCTGGCCGCGTACTTGTTGTTGCTTTGTTTGGAGGCTGTGGTGGTCTCCA7270.11502491151625617No Hit
GACCTAGGACGGTCAGTCTGGTCCCTCCTCCGAAGACCAAAGTGCGGGAG6990.1105947911277346No Hit
TATTTATTGAGGGTATATTGAGTGCAGGGAGAAGGGCTTGATGCCTTGGG6900.10917082385999553No Hit
GAGTTTATTCAGGGGTGGGGACAGGCGGGTGGCTCAGTAGCAGGTGCCGT6720.10632288932451739No Hit
ACACTGGTGTGTCTCATAAGTGACTTCTACCCGGGAGCCGTGACAGTGGC6700.10600645215390872No Hit
GTCCTAGGTCAGCCCAAGGCTGCCCCCTCGGTCACTCTGTTCCCGCCCTC6680.10569001498330004No Hit
GCTCTAGTCTCCCGTGGTGGGGGTGAGGGTTGAGAACCTATGAAGATTCT6630.10489892205677832No Hit
GTTCCCGCCCTCCTCTGAGGAGCTTCTAGCCAACAAGGCCACACTGGTGT6620.10474070347147398No Hit
CCCTTCATGCGTGACCTGGCAGCTGTAGCTTCTGTGGGACTTCCACTGCTCAGGCGTCAGGCTCAGATAGCTG6540.10347495478903926No Hit

[OK]Adapter Content

Adapter graph

[WARN]Kmer Content

Kmer graph

SequenceCountPValueObs/Exp MaxMax Obs/Exp Position
CGTGCGT503.2960577E-452.4645270
CGAATTC200.007156740452.11319742
AACGTCG200.00737848251.71261639
GCCGTTA352.5960217E-548.1857315
GTACGGT350.001507186338.53943314
GTATCAA25650.038.0068051
CCGTTAA451.1427869E-437.48075516
GTGGTAT4100.035.3781781
TATCAAC29850.032.6358572
ATCAACG31400.030.91753
AATGGTA2351.09139364E-1030.69732770
TCAACGC31700.030.6249054
CTAATGG1951.8499122E-830.26799470
AACGCAG32300.030.1675436
CAACGCA32300.030.1651555
GGGTATA6450.029.2872891
CGCAGAG34000.028.6591668
AGAGTAC34200.028.49156611
CAGAGTA34300.028.31020410
ACGCAGA34450.028.284817
Produced by FastQC (version 0.11.5)