FastQCFastQC Report
Sat 23 Apr 2022
EGAF00004684411

Summary

[OK]Basic Statistics

MeasureValue
FilenameEGAF00004684411
File typeConventional base calls
EncodingSanger / Illumina 1.9
Total Sequences887241
Sequences flagged as poor quality0
Sequence length20-76
%GC56

[OK]Per base sequence quality

Per base quality graph

[OK]Per tile sequence quality

Per base quality graph

[OK]Per sequence quality scores

Per Sequence quality graph

[FAIL]Per base sequence content

Per base sequence content

[FAIL]Per sequence GC content

Per sequence GC content graph

[OK]Per base N content

N content graph

[WARN]Sequence Length Distribution

Sequence length distribution

[FAIL]Sequence Duplication Levels

Duplication level graph

[WARN]Overrepresented sequences

SequenceCountPercentagePossible Source
GGGGGGGGGGGGGGGGGGGGGGGGGGGGGGGGGGGGGGGGGGGGGGGGGG57740.6507814674930488No Hit
CTCCTGGGAGTTACCCGATTGGAGGGCGTTATCCACCTTCCACTGTACTTTGGCCTCTCTGGGATAGAAGTTATT26820.30228539934471016No Hit
CTCTAACACTCTCCCCTGTTGAAGCTCTTTGTGACGGGCGAGCTCAGGCC21840.24615634309054696No Hit
GAAATACACAGCCGAGTCCGCGGCGTTCACAGAGCTCAACTTCAGGGAGA21660.24412758202111942No Hit
GTTAGGACCCAGAGGGAACCATGGAAACCCCAGCGCAGCTTCTCTTCCTCCTGCTACTCTGGCTCCCAGATACCA21610.24356403727961176No Hit
ACCCTGGCCAGGCTCCCAGGCTCCTCATCTATGGTGCATCCAGCAGGGCC17970.2025379800978539No Hit
GGCGTAGACTTTGTGTTTCTCGTAGTCTGCTTTGCTCAGCGTCAGGGTGC17860.20129818166653704No Hit
GTGTATTTCTGTGCGAGAGGCCCGTTTTTATATGGTTCGGGGAGACCTCCGTTTGACTCCTGGGGCCAGGGAACC17310.19509918950995278No Hit
GTGTGACCTGGAGCGAAAGCGGACAGGGCGTGACCGCCAGAAACTTCCCACCCAGCCAGGATGCCTCCGGGGAC16940.19092895842279606No Hit
CTCCCAGGAGAGTGTCACAGAGCAGGACAGCAAGGACAGCACCTACAGCC15670.17661492198850143No Hit
GTCATGGACCTCCTGCACAAGAACATGAAACACCTGTGGTTCTTCCTCCTCCTGGTGGCAGCTCCCAGATGGGTC15430.17390990722926464No Hit
GTGCAGTGACAGTCGGGGGTGGCAGCATGAGGGAGATGGGGTAGGTGGAG15230.17165572826323403No Hit
GTGTACAGGTCCCCGGAGGCATCCTGGCTGGGTGGGAAGTTTCTGGCGGT14960.16861258665909262No Hit
GTCACATCCTGGCTGGGATTCGTGTAGTGCTTCACGTGGCATGTCACGGA14550.16399151977872978No Hit
CAACAGAGGCAGTTCCAGATTTCAACTGCTCATCAGATGGCGGGAAGATG14510.16354068398552365No Hit
GTCCTGCTCTGTGACACTCTCCTGGGAGTTACCCGATTGGAGGGCGTTATCCACCTTCCACTGTACTTTGGCCT14320.16139921396779455No Hit
GTTCAAGGACCACCTGAGCGTGACCTCTGTGGCTGCTACAGCGTGTCCAG14270.1608356692262869No Hit
CATCTATGGTGCATCCAGCAGGGCCACTGGCATCCCAGACAGGTTCAGTGGCAGTGGCTCTGGGACAGACTTCA13800.15553834865611485No Hit
GTCCAGGGCTTCTTCCCCCAGGAGCCACTCAGTGTGACCTGGAGCGAAAGCGGACAGGGCGTGACCGCCAGAAA13780.15531293075951177No Hit
GTCACTGCACCGACCGGCCCTCGAGGACCTGCTCTTAGGTTCAGAAGCGAACCTCACGTGCACACTGACCGGC13580.15305875179348116No Hit
GGCTGGAACTGAGGAGCAGGTGGGGGCACTTCTCCCTCTAACACTCTCCC13540.152607916000275No Hit
CTCCTGCAGGGCCAGTCAGAGTGTTAGCAGCAGCTACTTAGCCTGGTACC13510.1522697891553704No Hit
GCGTCAGGGTGCTGCTGAGGCTGTAGGTGCTGTCCTTGCTGTCCTGCTCT13170.14843768491311832No Hit
GCCCCAAGGTCTTCCCGCTGAGCCTCTGCAGCACCCAGCCAGATGGGAAC12820.14449287172256467No Hit
GTACAGTGGAAGGTGGATAACGCCCTCCAATCGGGTAACTCCCAGGAGAG12660.14268952854974015No Hit
GTCTTTGTCTCCAGGGGAAAGAGCCACCCTCTCCTGCAGGGCCAGTCAGA12390.13964638694559878No Hit
GTCTCCAGGCACCCTGTCTTTGTCTCCAGGGGAAAGAGCCACCCTCTCCTGCAGGGCCAGTCAGAGTGTTAGCAG12370.1394209690489957No Hit
GGATGGGAGGGGGTCAGGCTGGAACTGAGGAGCAGGTGGGGGCACTTCTCCCTCTAACACTCTCCCCTGTTGAAG12320.13885742430748804No Hit
AGGTCACACTGAGTGGCTCCTGGGGGAAGAAGCCCTGGACCAGGCAGGCG12200.13750491692786965No Hit
GTCTACGCCTGCGAAGTCACCCATCAGGGCCTGAGCTCGCCCGTCACAAA11940.1345744842720298No Hit
GTTTTACTGTCAGCAATATGGTAGTTCACCCTACACTTTTGGCCAGGGGA11840.1334473947890145No Hit
GGATGTGACTGTGCCCTGCCCAGTTCCCTCAACTCCACCTACCCCATCTCCCTCAACTCCACCTACCCCATCTCC11810.1331092679441099No Hit
TCCTTGGGGCTGAAGCCACGTGCCAGGCACGTCAGCGTCACCAGCTCGTT11590.13062967108147616No Hit
CTCCAGGCACCCTGTCTTTGTCTCCAGGGGAAAGAGCCACCCTCTCCTGC11460.12916445475355626No Hit
GTGCCTAGCCGGCAAGTCCGTGACATGCCACGTGAAGCACTACACGAATCCCAGCCAGGATGTGACTGTGCCCTG11420.12871361896035013No Hit
GGGTTGTGCTGGTACCAGGCTAAGTAGCTGCTGCTAACACTCTGACTGGCCCTGCAGGAGAGGGTGGCTCTTTCC11390.12837549211544552No Hit
CGTGCACACTGACCGGCCTGAGAGATGCCTCAGGTGTCACCTTCACCTGGACGCCCTCAAGTGGGAAGAGCG11340.12781194737393786No Hit
GACTTCACTCTCACCATCAGCAGACTGGAGCCTGAAGACTTTGCTGTGTTTTACTGTCAGCAATATGGTAGTTCA11260.12691027578752562No Hit
CTCTGGGATAGAAGTTATTCAGCAGGCACACAACAGAGGCAGTTCCAGAT11250.12679756683922405No Hit
CAGTAGACCCGTCCAAGGACCAGTTCTCCCTGAAGTTGAGCTCTGTGAACGCCGCGGACTCGGCTGTGTATTT11100.12510693261470107No Hit
CCACTGTACTTTGGCCTCTCTGGGATAGAAGTTATTCAGCAGGCACACAACAGAGGCAGTTCCAGATTTCAACTG10920.12307817154527348No Hit
CAGTTAGGACCCAGAGGGAACCATGGAAACCCCAGCGCAGCTTCTCTTCCTCCTGCTACTCTGGCTCCCAGATAC10900.12285275364867043No Hit
GGCTGTAGGTGCTGTCCTTGCTGTCCTGCTCTGTGACACTCTCCTGGGAGTTACCCGATTGGAGGGCGTTATCCA10900.12285275364867043No Hit
CTACTGGACCTGGATCCGCCAGTCCCCAGGGAAGGGGCCGGAGTGGATTGGGGAAATCAGTCACAGTGGAATCAC10780.12150024626905204No Hit
GTCTTCATCTTCCCGCCATCTGATGAGCAGTTGAAATCTGGAACTGCCTCTGTTGTGTGCCTGCTGAATAACTT10770.12138753732075051No Hit
GGTCTACTGATATGGTGACTCGACTCTTGAGTGACGGGTTGTAGCTGGTG10730.12093670152754438No Hit
GTCTTGGACTCGGGGTAGGCAGCAGTGCAAGTGAAGGTCTTCCCATGGTT10720.12082399257924284No Hit
CAATAGGGGTAGGTCCCCTGTGGAAAAAGGGTCAGAGGCCAAAGGATGGGAGGGGGTCAGGCTGGAACTGAGGAG10580.11924606730302138No Hit
GCACAACCCTGGCCAGGCTCCCAGGCTCCTCATCTATGGTGCATCCAGCAGGGCCACTGGCATCCCAGACAGGTT10520.11856981361321219No Hit
GTGTCTGGTGGGTCCTTCAGTGGTTACTACTGGACCTGGATCCGCCAGTC10350.11665376149208614No Hit
GTCCAAGACCCCGCTAACCGCCACCCTCTCAAAATCCGGAAACACATTCC10330.11642834359548307No Hit
GAGTGAAGTCTGTCCCAGAGCCACTGCCACTGAACCTGTCTGGGATGCCA9850.1110183140770095No Hit
GTGTAGGGTGAACTACCATATTGCTGACAGTAAAACACAGCAAAGTCTTC9790.1103420603872003No Hit
ATATGGTAGTTCACCCTACACTTTTGGCCAGGGGACCAAGCTGGAGATCAAACGAACTGTGGCTGCACCAT9760.11000393354229573No Hit
CCTGTACACCACGAGCAGCCAGCTGACCCTGCCGGCCACACAGTGCCTAG9710.10944038880078806No Hit
GTGCAGCCACAGTTCGTTTGATCTCCAGCTTGGTCCCCTGGCCAAAAGTG9660.10887684405928039No Hit
CCATAGATGAGGAGCCTGGGAGCCTGGCCAGGGTTGTGCTGGTACCAGGCTAAGTAGCTGCTGCTAACACTCTGA9630.1085387172143758No Hit
GTGTGCACGTGAGGTTCGCTTCTGAACCTAAGAGCAGGTCCTCGAGGGCC9600.1082005903694712No Hit
CCTTGGGGCTGGTCGGGGATGCTGAGGAGACGGTGACCGGGGTTCCCTGGCCCCAGGAGTCAAACGGAGGTCTCC9600.1082005903694712No Hit
GGTTACTACTGGACCTGGATCCGCCAGTCCCCAGGGAAGGGGCCGGAGTG9570.1078624635245666No Hit
GATGAAGACAGATGGTGCAGCCACAGTTCGTTTGATCTCCAGCTTGGTCC9500.10707350088645588No Hit
GTGGCTGCACCATCTGTCTTCATCTTCCCGCCATCTGATGAGCAGTTGAAATCTGGAACTGCCTCTGTTGTGTGC9460.10662266509324976No Hit
GTCCTGTCCCAGGTGCAGTTACAGCAGTGGGGCGCAGGACTGTTGAAGCC9400.10594641140344056No Hit
GCTCAGTTAGGACCCAGAGGGAACCATGGAAACCCCAGCGCAGCTTCTCT9350.10538286666193289No Hit
GAGTGGCTCCTGGGGGAAGAAGCCCTGGACCAGGCAGGCGATGACCACGT9350.10538286666193289No Hit
GTATGCTGGTCACAGCGAAGGTGGTGGTGCCCTGGCTGGGCTCCTGCCGG9330.10515744876532981No Hit
GCTCAGTAGCAGGTGCCGTCCACCTCCGCCATGACAACAGACACATTGAC9310.10493203086872677No Hit
GAGCAGGACAGCAAGGACAGCACCTACAGCCTCAGCAGCACCCTGACGCT9300.10481932192042522No Hit
GTTGAAGCCTTCGGAGACCCTGTCCCTCAGCTGCGTTGTGTCTGGTGGGT9250.10425577717891757No Hit
GAGTTTATTCAGGGGTGGGGACAGGCGGGCGGCTCAGTAGCAGGTGCCGT9210.10380494138571142No Hit
CTGCAGCACCCAGCCAGATGGGAACGTGGTCATCGCCTGCCTGGTCCAGG9080.10233972505779151No Hit
GGGCGTGACCGCCAGAAACTTCCCACCCAGCCAGGATGCCTCCGGGGACCTGTACACCACGAGCAGCCAGCTGAC8950.1008745087298716No Hit

[OK]Adapter Content

Adapter graph

[FAIL]Kmer Content

Kmer graph

SequenceCountPValueObs/Exp MaxMax Obs/Exp Position
GTATCAA20900.041.6617931
GTCTAGA451.0888155E-437.7921681
TATCAAC23250.037.5589642
ATCAACG24200.036.0804753
TCAACGC25150.034.9897424
GGTATCA7250.034.7166671
TTGCTCG400.002743629534.1130429
ACGCAGA26200.033.5893757
CGCAGAG26150.033.5236638
AGAGTAC26350.033.2692111
GCAGAGT27200.032.2277349
TCACGGT450.00443142230.93185245
CCTATAA553.5606406E-430.889492
CAACGCA29650.029.6809985
AACGCAG29550.029.6664586
CAGAGTA29700.029.6310310
TGTTAGA5400.028.90386670
GACAGCC2150.028.475961
AGGACGA500.00693529828.22316454
ACGGGAA3300.027.81151616
Produced by FastQC (version 0.11.5)