FastQCFastQC Report
Wed 27 Apr 2022
EGAF00004684412

Summary

[OK]Basic Statistics

MeasureValue
FilenameEGAF00004684412
File typeConventional base calls
EncodingSanger / Illumina 1.9
Total Sequences994353
Sequences flagged as poor quality0
Sequence length20-76
%GC57

[OK]Per base sequence quality

Per base quality graph

[OK]Per tile sequence quality

Per tile quality graph

[OK]Per sequence quality scores

Per Sequence quality graph

[FAIL]Per base sequence content

Per base sequence content

[FAIL]Per sequence GC content

Per sequence GC content graph

[OK]Per base N content

N content graph

[WARN]Sequence Length Distribution

Sequence length distribution

[FAIL]Sequence Duplication Levels

Duplication level graph

[WARN]Overrepresented sequences

SequenceCountPercentagePossible Source
GTAATATACGGCCGTGTCTTCGGCTCTCAGGCTGTTCAGTTGCAAATACA40470.4069983195102745No Hit
GCCCAAGGCTGCCCCCTCGGTCACTCTGTTCCCGCCCTCCTCTGAGGAGC38830.39050518276708573No Hit
GCCTTGGGCTGACCTAGGACGGTCAGCTTGGTCCCTCCGCCGAACACGTG37090.3730063669541903No Hit
GTGTGGTGGTCTCCACTCCCGCCTTGACGGGGCTGCTATCTGCCTTCCAG32470.32654399393374384No Hit
GACCTAGGACGGTCAGCTTGGTCCCTCCGCCGAACACGTGAATGCCAGTGCCCCAGGTCTGACAGAAATAGTCAG31970.32151559858521067No Hit
CCTCTGAGGAGCTTCAAGCCAACAAGGCCACACTGGTGTGTCTCATAAGT28220.28380263347121193No Hit
GTATATTACTGTGCGAAAGTAGGATACAAAAGTGGTAACGTCCAACACTGGGGCCAGGGCACCCTGGTCACCGT26290.2643930274258739No Hit
GCTCTGGGGAGTCTGCACCATGGCTTGGACCCCACTCCTCTTCCTCACCCTCCTCCTCCACTGCACAGGGTCTCT25490.25634759486822084No Hit
GTGTTAAGTTTCATCAAGTAGCGAGGGCCCTTCTCTGGCTGCAGCTGATGCCATGCGATGGCGTAGGTGCTGTGC25070.25212374277545296No Hit
CCTCAGAGGAGGGCGGGAACAGAGTGACCGAGGGGGCAGCCTTGGGCTGACCTAGGACGGTCAGCTTGGTCCCTC24610.24749761905480247No Hit
GCCCCAGAACTGGAGCCTGAGAAGCGATCAGGGATCCCGTCCCCCTTGGT24200.24337433486900528No Hit
GTTCTGGGGCTGAGCGCAACCTCATCATCTCCAGTCTCCAGTCTGACGATGAGGCTGACTATTTCTGTCAGAC24150.24287149533415198No Hit
GTGCAGGGAGAAGGGCTTGATGCCTTGGGGTGGGAGGAGAGACCCCTCCCCTGGGATCCTGCAGCTCTAGTCTCC23540.23673685300894148No Hit
CCTATGAACATTCTGTAGGGGCCACTGTCTTCTCCACGGTGCTCCCTTCA23470.23603287766014686No Hit
TCCTAGGTCAGCCCAAGGCTGCCCCCTCGGTCACTCTGTTCCCGCCCTCCTCTGAGGAGCTTCAAGCCAACAAGG23080.232110729288291No Hit
GGGTCTCTCTCCCAGCTTGTGCTGACTCAATCGCCCTCTGCCTCTGCCTCCCTGGGAGCCTCGGTCAAGCTCAC22920.23050164277676036No Hit
CTTATACACATCTCCGAGCCCACGAGACTAGCGCTCATCTCGTATGCCGT21390.21511475301024888No Hit
GTCTTCTCCACGGTGCTCCCTTCATGCGTGACCTGGCAGCTGTAGCTTTT20810.20928181440595042No Hit
GGGTTTATTGAGTGCAGGGAGAAGGGCTTGATGCCTTGGGGTGGGAGGAGAGACCCCTCCCCTGGGATCCTGCAG20620.2073710241735078No Hit
CACCAAGGGGGACGGGATCCCTGATCGCTTCTCAGGCTCCAGTTCTGGGG19680.19791764091826544No Hit
GTCCTAGGTCAGCCCAAGGCTGCCCCCTCGGTCACTCTGTTCCCGCCCTC18570.1867546032445218No Hit
GGACTGAACAGAGAGAACTCACCATGGAGTTTGGGCTGAGCTGGCTTTTT18570.1867546032445218No Hit
CTCTGTTCCCGCCCTCCTCTGAGGAGCTTCAAGCCAACAAGGCCACACTGGTGTGTCTCATAAGTGACTTCTACC18380.18484381301207922No Hit
GTGCTCCCTTCATGCGTGACCTGGCAGCTGTAGCTTTTGTGGGACTTCCACTGCTCAGGCGTCAGGCTCAGGTAG18100.18202791161690066No Hit
GTTTGGAGGGTGTGGTGGTCTCCACTCCCGCCTTGACGGGGCTGCTATCTGCCTTCCAGGCCACTGTCACGGCTC17950.18051939301234068No Hit
GTCACATCCTGGCTGGGATTCGTGTAGTGCTTCACGTGGCATGTCACGGA17870.17971484975657537No Hit
ACTTAACACTGATGGCAGCCACACCAAGGGGGACGGGATCCCTGATCGCT17740.17840746696595677No Hit
CACCAGTGTGGCCTTGTTGGCTTGAAGCTCCTCAGAGGAGGGCGGGAACAGAGTGACCGAGGGGGCAGCCTTGGG17180.1727756641755996No Hit
GTGCAGTGACAGTCGGGGGTGGCAGCATGAGGGAGATGGGGTAGGTGGAG17100.1719711209198343No Hit
CTTATGAGACACACCAGTGTGGCCTTGTTGGCTTGAAGCTCCTCAGAGGA16900.16995976278042105No Hit
GCTCAGGCGTCAGGCTCAGGTAGCTGCTGGCCGCGTACTTGTTGTTGCTTTGTTTGGAGGGTGTGGTGGTCTCCA16830.1692557874316264No Hit
CTCCTGTGCAGCCTCTGGATTCACCTTTAGCAGCTATGCCATGAGCTGGGTCCGCCAGGCTCCAGGGAAGGGG16580.1667415897573598No Hit
GAGAAGGGCTTGATGCCTTGGGGTGGGAGGAGAGACCCCTCCCCTGGGAT16360.1645290958040052No Hit
GCACAGGGTCTCTCTCCCAGCTTGTGCTGACTCAATCGCCCTCTGCCTCTGCCTCCCTGGGAGCCTCGGTCAAG16310.1640262562691519No Hit
GTGTTGGACGTTACCACTTTTGTATCCTACTTTCGCACAGTAATATACGGCCGTGTCTTCGGCTCTCAGGCTGTT16130.16221603394367995No Hit
CCTGCACTCTGAGCAGTGGGCACAGCACCTACGCCATCGCATGGCATCAG16090.1618137623157973No Hit
GTGTACAGGTCCCCGGAGGCATCCTGGCTGGGTGGGAAGTTTCTGGCGGT15900.1599029720833547No Hit
GGTTTATTGAGTGCAGGGAGAAGGGCTTGATGCCTTGGGGTGGGAGGAGAGACCCCTCCCCTGGGATCCTGCAG15890.15980240417638403No Hit
CTTCATGCGTGACCTGGCAGCTGTAGCTTTTGTGGGACTTCCACTGCTCAGGCGTCAGGCTCAGGTAGCTGCTGG15720.15809274975788276No Hit
CCTCCTCCACTGCACAGGGTCTCTCTCCCAGCTTGTGCTGACTCAATCGCCCTCTGCCTCTGCCTCCCTGGGAGC15440.1552768483627042No Hit
GTACGGGAGAGCTCTGGGGAGTCTGCACCATGGCTTGGACCCCACTCCTCTTCCTCACCCTCCTCCTCCACTGCA15250.15336605813026158No Hit
CACCTGCACTCTGAGCAGTGGGCACAGCACCTACGCCATCGCATGGCATCAGCTGCAGCCAGAGAAGGGCCCTCG15030.151153564176907No Hit
CCCTTCATGCGTGACCTGGCAGCTGTAGCTTTTGTGGGACTTCCACTGCTCAGGCGTCAGGCTCAGGTAGCTG14730.1481365269677871No Hit
CCACCACACCCTCCAAACAAAGCAACAACAAGTACGCGGCCAGCAGCTAC14550.14632630464231516No Hit
ACACTGGTGTGTCTCATAAGTGACTTCTACCCGGGAGCCGTGACAGTGGC14410.14491835394472588No Hit
AGGTCACACTGAGTGGCTCCTGGGGGAAGAAGCCCTGGACCAGGCAGGCG14190.14270585999137128No Hit
GGCTCAGGTAGCTGCTGGCCGCGTACTTGTTGTTGCTTTGTTTGGAGGGTGTGGTGGTCTCCACTCCCGCCTTGA14110.14190131673560596No Hit
ACCTAGGACGGTCAGCTTGGTCCCTCCGCCGAACACGTGAATGCCAGTGCCCCAGGTCTGACAGAAATAGTCAGC14020.14099620557287No Hit
CTCCCAGCTTGTGCTGACTCAATCGCCCTCTGCCTCTGCCTCCCTGGGAGCCTCGGTCAAGCTCACCTGCACT13470.13546497068948352No Hit
CCACTGCACAGGGTCTCTCTCCCAGCTTGTGCTGACTCAATCGCCCTCTGCCTCTGCCTCCCTGGGAGCCTCGGT13370.13445929161977688No Hit
GCTATCTGCCTTCCAGGCCACTGTCACGGCTCCCGGGTAGAAGTCACTTA13230.1330513409221876No Hit
GGCTTGGACCCCACTCCTCTTCCTCACCCTCCTCCTCCACTGCACAGGGT13160.13234736557339297No Hit
GCTCTAGTCTCCCGTGGTGGGGGGTGAGGGTTGAGAACCTATGAACATTC13020.13093941487580366No Hit
GCCTTGGGGTGGGAGGAGAGACCCCTCCCCTGGGATCCTGCAGCTCTAGT12830.12902862464336107No Hit
GTCACTGCACCGACCGGCCCTCGAGGACCTGCTCTTAGGTTCAGAAGCGAACCTCACGTGCACACTGACCGGC12770.12842521720153707No Hit
TCCTTGGGGCTGAAGCCACGTGCCAGGCACGTCAGCGTCACCAGCTCGTT12760.1283246492945664No Hit
GTATGCTGGTCACAGCGAAGGTGGTGGTGCCCTGGCTGGGCTCCTGCCGG12760.1283246492945664No Hit
GTGTGACCTGGAGCGAAAGCGGACAGGGCGTGACCGCCAGAAACTTCCCACCCAGCCAGGATGCCTCCGGGGAC12620.12691669859697713No Hit
GGAACAGAGTGACCGAGGGGGCAGCCTTGGGCTGACCTAGGACGGTCAGC12600.1267155627830358No Hit
GTTCCCGCCCTCCTCTGAGGAGCTTCAAGCCAACAAGGCCACACTGGTGT12500.12570988371332917No Hit
GAGTGACCGAGGGGGCAGCCTTGGGCTGACCTAGGACGGTCAGCTTGGTC12320.12389966138785724No Hit
GTCCAGGGCTTCTTCCCCCAGGAGCCACTCAGTGTGACCTGGAGCGAAAGCGGACAGGGCGTGACCGCCAGAAA12160.12229057487632661No Hit
GAGTACGGGAGAGCTCTGGGGAGTCTGCACCATGGCTTGGACCCCACTCCTCTTCCTCACCCTCCTCCTCCACTG12140.12208943906238529No Hit
CCCTTGGTGTGGCTGCCATCAGTGTTAAGTTTCATCAAGTAGCGAGGGCC12040.12108375999267866No Hit
GACTTCCACTGCTCAGGCGTCAGGCTCAGGTAGCTGCTGGCCGCGTACTT11900.11967580929508938No Hit
GAAATAGTCAGCCTCATCGTCAGACTGGAGACTGGAGATGATGAGGTTGC11630.11696047580688146No Hit
GTTCAAGGACCACCTGAGCGTGACCTCTGTGGCTGCTACAGCGTGTCCAG11550.11615593255111616No Hit
GTCTTGGACTCGGGGTAGGCAGCAGTGCAAGTGAAGGTCTTCCCATGGTT11530.11595479673717482No Hit
CTCCAGTTCTGGGGCTGAGCGCAACCTCATCATCTCCAGTCTCCAGTCTG11440.11504968557443886No Hit
GGCACATACTACGCAGACTCCGTGAAGGGCCGGTTCACCATCTCCAGAGACAATTCCAAGAACACGCTGTATTTG11440.11504968557443886No Hit
GGTGTGTCTCATAAGTGACTTCTACCCGGGAGCCGTGACAGTGGCCTGGA11340.11404400650473223No Hit
GACTATTTCTGTCAGACCTGGGGCACTGGCATTCACGTGTTCGGCGGAGGGACCAAGCTGACCGTCCTAGGTCAG11310.11374230278382022No Hit
GTATCAACGCAGAGTACGGGAGAGCTCTGGGGAGTCTGCACCATGGCTTGGACCCCACTCCTCTTCCTCACCCTC11290.11354116696987891No Hit
GCCCCAAGGTCTTCCCGCTGAGCCTCTGCAGCACCCAGCCAGATGGGAAC11220.11283719162108426No Hit
GAGTGCAGGTGAGCTTGACCGAGGCTCCCAGGGAGGCAGAGGCAGAGGGC11190.11253548790017229No Hit
CCCCTGGGATCCTGCAGCTCTAGTCTCCCGTGGTGGGGGGTGAGGGTTGAGAACCTATGAACATTCTGTAGGGGC11170.11233435208623094No Hit
GAGTGGCTCCTGGGGGAAGAAGCCCTGGACCAGGCAGGCGATGACCACGT11030.11092640138864165No Hit
CTGTAGGGGCCACTGTCTTCTCCACGGTGCTCCCTTCATGCGTGACCTGG11030.11092640138864165No Hit
CTCCTCTTCCTCACCCTCCTCCTCCACTGCACAGGGTCTCTCTCCCAGCTTGTGCTGACTCAATCGCCCTCTGC11020.110825833481671No Hit
GCTCAGTAGCAGGTGCCGTCCACCTCCGCCATGACAACAGACACATTGAC10920.10982015441196437No Hit
GCTAGGCACTGTGTGGCCGGCAGGGTCAGCTGGCTGCTCGTGGTGTACAG10820.10881447534225773No Hit
GGGCTGCTATCTGCCTTCCAGGCCACTGTCACGGCTCCCGGGTAGAAGTCACTTATGAGACACACCAGTGTGGC10740.10800993208649243No Hit
GCTTGGTCCCTCCGCCGAACACGTGAATGCCAGTGCCCCAGGTCTGACAGAAATAGTCAGCCTCATCGTCAGA10640.1070042530167858No Hit
CGTGAATGCCAGTGCCCCAGGTCTGACAGAAATAGTCAGCCTCATCGTCA10580.10640084557496181No Hit
GCCCTGGACCAGGCAGGCGATGACCACGTTCCCATCTGGCTGGGTGCTGC10520.10579743813313783No Hit
GAGAAGGGCCCTCGCTACTTGATGAAACTTAACACTGATGGCAGCCACACCAAGGGGGACGGGATCCCTGATCG10440.10499289487737253No Hit
TCCTCACCCTCCTCCTCCACTGCACAGGGTCTCTCTCCCAGCTTGTGCTGACTCAATCGCCCTCTGCCTCTGCCT10430.10489232697040186No Hit
GGATGTGACTGTGCCCTGCCCAGTTCCCTCAACTCCACCTACCCCATCTCCCTCAACTCCACCTACCCCATCTCC10320.10378607999372456No Hit
TTCCAGGCCACTGTCACGGCTCCCGGGTAGAAGTCACTTATGAGACACACCAGTGTGGCCTTGTTGGCTTGAAG10280.10338380836584192No Hit
GTGCCTAGCCGGCAAGTCCGTGACATGCCACGTGAAGCACTACACGAATCCCAGCCAGGATGTGACTGTGCCCTG10210.10267983301704728No Hit
GTGTGCACGTGAGGTTCGCTTCTGAACCTAAGAGCAGGTCCTCGAGGGCC10190.10247869720310593No Hit
CTCCACTCCCGCCTTGACGGGGCTGCTATCTGCCTTCCAGGCCACTGTCA10150.1020764255752233No Hit
GGTAGCTGCTGGCCGCGTACTTGTTGTTGCTTTGTTTGGAGGGTGTGGTGGTCTCCACTCCCGCCTTGACGGGG10100.10157358604036996No Hit
GAACTGGAGCCTGAGAAGCGATCAGGGATCCCGTCCCCCTTGGTGTGGCT9960.10016563534278068No Hit

[OK]Adapter Content

Adapter graph

Produced by FastQC (version 0.11.9)