FastQCFastQC Report
Sat 23 Apr 2022
EGAF00004684414

Summary

[OK]Basic Statistics

MeasureValue
FilenameEGAF00004684414
File typeConventional base calls
EncodingSanger / Illumina 1.9
Total Sequences1165405
Sequences flagged as poor quality0
Sequence length20-76
%GC53

[OK]Per base sequence quality

Per base quality graph

[OK]Per tile sequence quality

Per base quality graph

[OK]Per sequence quality scores

Per Sequence quality graph

[FAIL]Per base sequence content

Per base sequence content

[FAIL]Per sequence GC content

Per sequence GC content graph

[OK]Per base N content

N content graph

[WARN]Sequence Length Distribution

Sequence length distribution

[FAIL]Sequence Duplication Levels

Duplication level graph

[WARN]Overrepresented sequences

SequenceCountPercentagePossible Source
GTATAAAAGTTTGTACACTTTTGGCCCGGGGACCAAGCTGGAGATCAAACGAACTGTGGCTGCACCATCTGTCTT61520.5278851558042054No Hit
CTCCTGGGAGTTACCCGATTGGAGGGCGTTATCCACCTTCCACTGTACTTTGGCCTCTCTGGGATAGAAGTTATT59110.5072056495381434No Hit
CTCTAACACTCTCCCCTGTTGAAGCTCTTTGTGACGGGCGAGCTCAGGCC58890.5053178937794157No Hit
CTTTTATACTGTTGGCAGTAATAAGTTGCAAAATCATCAGGCTGCAGGCTGCTGATGATGAGAGTGAATTCTGTC50290.43152380502915294No Hit
GGCTGGAACTGAGGAGCAGGTGGGGGCACTTCTCCCTCTAACACTCTCCC40900.350950956963459No Hit
GGCGTAGACTTTGTGTTTCTCGTAGTCTGCTTTGCTCAGCGTCAGGGTGC39990.343142512688722No Hit
AGTCAGGACACAGCATGGACATGAGGGTCCCCGCTCAGCTCCTGGGGCTC39460.33859473745178714No Hit
ATTAGTAACTGGTTGGCCTGGTATCAGCAGAAACCAGGGAAAGCCCCTAA38020.3262385179401152No Hit
CTTTTGGCCCGGGGACCAAGCTGGAGATCAAACGAACTGTGGCTGCACCA37070.31808684534560944No Hit
CAATAGGGGTAGGTCCCCTGTGGAAAAAGGGTCAGAGGCCAAAGGATGGGAGGGGGTCAGGCTGGAACTGAGGAG36230.3108790506304675No Hit
CTCCCAGGAGAGTGTCACAGAGCAGGACAGCAAGGACAGCACCTACAGCC36100.3097635585912194No Hit
CAACAGAGGCAGTTCCAGATTTCAACTGCTCATCAGATGGCGGGAAGATG35710.3064170824734749No Hit
GGATGGGAGGGGGTCAGGCTGGAACTGAGGAGCAGGTGGGGGCACTTCTCCCTCTAACACTCTCCCCTGTTGAAG34250.29388924880191863No Hit
GTCTGCATCTGTCGGAGACAGAGTCACCATCACTTGCCGGGCCAGTCAGA34130.29285956384261264No Hit
GTACAGTGGAAGGTGGATAACGCCCTCCAATCGGGTAACTCCCAGGAGAG33730.289427280644926No Hit
GTCTACGCCTGCGAAGTCACCCATCAGGGCCTGAGCTCGCCCGTCACAAA33330.2859949974472394No Hit
CCTTCCACCCTGTCTGCATCTGTCGGAGACAGAGTCACCATCACTTGCCG32280.2769852540533119No Hit
GTCCTGCTCTGTGACACTCTCCTGGGAGTTACCCGATTGGAGGGCGTTATCCACCTTCCACTGTACTTTGGCCT32100.2754407266143529No Hit
GCGTCAGGGTGCTGCTGAGGCTGTAGGTGCTGTCCTTGCTGTCCTGCTCT31150.26728905401984715No Hit
GACTATTAGTAACTGGTTGGCCTGGTATCAGCAGAAACCAGGGAAAGCCCCTAAACTCCTGATCTATAAGACGT31100.26686001862013636No Hit
GCATGGACATGAGGGTCCCCGCTCAGCTCCTGGGGCTCCTGCTGCTCTGG30610.2626554717029702No Hit
GTCTTCATCTTCCCGCCATCTGATGAGCAGTTGAAATCTGGAACTGCCTCTGTTGTGTGCCTGCTGAATAACTT28560.24506502031482616No Hit
GGCCAAAAGTGTACAAACTTTTATACTGTTGGCAGTAATAAGTTGCAAAA27980.24008820967818056No Hit
GAATTCACTCTCATCATCAGCAGCCTGCAGCCTGATGATTTTGCAACTTA27590.2367417335604361No Hit
GTCGGAGACAGAGTCACCATCACTTGCCGGGCCAGTCAGACTATTAGTAACTGGTTGGCCTGGTATCAGCAGAAA26480.22721714768685564No Hit
CCACTGTACTTTGGCCTCTCTGGGATAGAAGTTATTCAGCAGGCACACAACAGAGGCAGTTCCAGATTTCAACTG26430.2267881122871448No Hit
GTGGCTGCACCATCTGTCTTCATCTTCCCGCCATCTGATGAGCAGTTGAAATCTGGAACTGCCTCTGTTGTGTGC26110.22404228572899548No Hit
CTCTGGGATAGAAGTTATTCAGCAGGCACACAACAGAGGCAGTTCCAGAT25920.22241195121009433No Hit
CAGCATGGACATGAGGGTCCCCGCTCAGCTCCTGGGGCTCCTGCTGCTCTGGCTCCCAGGTGCCAAATGTGACAT25620.21983773881182936No Hit
GGACACAGCATGGACATGAGGGTCCCCGCTCAGCTCCTGGGGCTCCTGCT24880.21348801489610908No Hit
GGCTGTAGGTGCTGTCCTTGCTGTCCTGCTCTGTGACACTCTCCTGGGAGTTACCCGATTGGAGGGCGTTATCCA24060.20645183434085146No Hit
GGCCAAAGGATGGGAGGGGGTCAGGCTGGAACTGAGGAGCAGGTGGGGGCACTTCTCCCTCTAACACTCTCCC23750.20379181486264433No Hit
ACCTTGAGGGGACCCCACTTTCTAAAGTAGACGTCTTATAGATCAGGAGTTTAGGGGCTTTCCCTGGTTTCTG23720.2035343936228178No Hit
GACCCAGTCAGGACACAGCATGGACATGAGGGTCCCCGCTCAGCTCCTGG23550.202075673263801No Hit
GCTCCTGGGGCTCCTGCTGCTCTGGCTCCCAGGTGCCAAATGTGACATCCAGATGACCCAGTCTCCTTCCACC23160.19872919714605652No Hit
GAGCAGGACAGCAAGGACAGCACCTACAGCCTCAGCAGCACCCTGACGCT21700.18620136347450028No Hit
ATCCCAGAGAGGCCAAAGTACAGTGGAAGGTGGATAACGCCCTCCAATCG21590.18525748559513644No Hit
CTAATAGTCTGACTGGCCCGGCAAGTGATGGTGACTCTGTCTCCGACAGA21230.18216843071721847No Hit
GTTACTAATAGTCTGACTGGCCCGGCAAGTGATGGTGACTCTGTCTCCGACAGATGCAGACAGGGTGGAAGGAGA20790.17839291919976316No Hit
GCTTTGCTCAGCGTCAGGGTGCTGCTGAGGCTGTAGGTGCTGTCCTTGCT20500.17590451388144038No Hit
CCTCAAGGTTCAGCGGCAGTGGATCTGGGACAGAATTCACTCTCATCATCAGCAGCCTGCAGCCTGATGATTTTG20180.17315868732329104No Hit
GGCCCTGATGGGTGACTTCGCAGGCGTAGACTTTGTGTTTCTCGTAGTCTGCTTTGCTCAGCGTCAGGGTGCTG20100.1724722306837537No Hit
GTTGTGTGCCTGCTGAATAACTTCTATCCCAGAGAGGCCAAAGTACAGTG19940.17109931740467904No Hit
GTCAGGACACAGCATGGACATGAGGGTCCCCGCTCAGCTCCTGGGGCTCC19810.1699838253654309No Hit
GATGAAGACAGATGGTGCAGCCACAGTTCGTTTGATCTCCAGCTTGGTCC19520.1674954200471081No Hit
GTTTAGGGGCTTTCCCTGGTTTCTGCTGATACCAGGCCAACCAGTTACTA19500.16732380588722373No Hit
GTCTCCTTCCACCCTGTCTGCATCTGTCGGAGACAGAGTCACCATCACTTGCCGGGCCAGTCAGACTATTAGTAA19500.16732380588722373No Hit
GTACTTTGGCCTCTCTGGGATAGAAGTTATTCAGCAGGCACACAACAGAGGCAGTTCCAGATTTCAACTGCTCAT18090.15522500761537833No Hit
GTCCTTGCTGTCCTGCTCTGTGACACTCTCCTGGGAGTTACCCGATTGGAGGGCGTTATCCACCTTCCACTGTA18070.15505339345549402No Hit
GTTGAAGCTCTTTGTGACGGGCGAGCTCAGGCCCTGATGGGTGACTTCGC17360.1489610907796002No Hit
CTTATACACATCTCCGAGCCCACGAGACCGATCAGTATCTCGTATGCCGT17360.1489610907796002No Hit
GTTGAAATCTGGAACTGCCTCTGTTGTGTGCCTGCTGAATAACTTCTATC17270.14818882706012074No Hit
GTGGAAAAAGGGTCAGAGGCCAAAGGATGGGAGGGGGTCAGGCTGGAACTGAGGAGCAGGTGGGGGCACTTCTCC16930.14527138634208708No Hit
GTGCAGCCACAGTTCGTTTGATCTCCAGCTTGGTCCCCGGGCCAAAAGTG16660.14295459518364861No Hit
GAGCAAAGCAGACTACGAGAAACACAAAGTCTACGCCTGCGAAGTCACCC16390.14063780402521012No Hit
CCTCTGTTGTGTGCCTGCTGAATAACTTCTATCCCAGAGAGGCCAAAGTA16360.14038038278538362No Hit
ACTTTAGAAAGTGGGGTCCCCTCAAGGTTCAGCGGCAGTGGATCTGGGACAGAATTCACTCTCATCATCAGCAGC16270.13960811906590412No Hit
ACTTATTACTGCCAACAGTATAAAAGTTTGTACACTTTTGGCCCGGGGAC16250.1394365049060198No Hit
CTTCTATCCCAGAGAGGCCAAAGTACAGTGGAAGGTGGATAACGCCCTCCAATCGGGTAACTCCCAGGAGAGTGT16190.1389216624263668No Hit
GGGTAGGTCCCCTGTGGAAAAAGGGTCAGAGGCCAAAGGATGGGAGGGGG16190.1389216624263668No Hit
GTGGAAGGTGGATAACGCCCTCCAATCGGGTAACTCCCAGGAGAGTGTCACAGAGCAGGACAGCAAGGACAGCAC15890.1363474500281018No Hit
CCCCTGTTGAAGCTCTTTGTGACGGGCGAGCTCAGGCCCTGATGGGTGACTTCGCAGGCGTAGACTTTGTGTTT15790.13548937922868015No Hit
ACCTTCCACTGTACTTTGGCCTCTCTGGGATAGAAGTTATTCAGCAGGCA15770.13531776506879584No Hit
CCGCAATAGGGGTAGGTCCCCTGTGGAAAAAGGGTCAGAGGCCAAAGGAT15580.13368743054989468No Hit
GGTGAAAGATGAGCTGGAGGACCGCAATAGGGGTAGGTCCCCTGTGGAAA15510.1330867809902995No Hit
GGTATCAGCAGAAACCAGGGAAAGCCCCTAAACTCCTGATCTATAAGACGTCTACTTTAGAAAGTGGGGTCCCCT15460.13265774559058868No Hit
CTTTGTGACGGGCGAGCTCAGGCCCTGATGGGTGACTTCGCAGGCGTAGA15430.13240032435076218No Hit
CATCATCAGCAGCCTGCAGCCTGATGATTTTGCAACTTATTACTGCCAACAGTATAAAAGTTTGTACACTTTTGG15270.13102741107168753No Hit
GGGTGGAAGGAGACTGGGTCATCTGGATGTCACATTTGGCACCTGGGAGCCAGAGCAGCAGGAGCCCCAGGAG15230.13068418275191887No Hit
GGACATGAGGGTCCCCGCTCAGCTCCTGGGGCTCCTGCTGCTCTGGCTCC15120.12974030487255506No Hit
GCCTCTGTTGTGTGCCTGCTGAATAACTTCTATCCCAGAGAGGCCAAAGTACAGTGGAAGGTGGATAACGCCCTC15080.12939707655278637No Hit
CCTCCAACATTAGCATAATTAAAGCCAAGGAGGAGGAGGGGGGTGAGGTG14990.12862481283330687No Hit
GAGGAGGAGGGGGGTGAGGTGAAAGATGAGCTGGAGGACCGCAATAGGGG14970.12845319867342253No Hit
GCACACAACAGAGGCAGTTCCAGATTTCAACTGCTCATCAGATGGCGGGA14900.1278525491138274No Hit
CCCCTAAACTCCTGATCTATAAGACGTCTACTTTAGAAAGTGGGGTCCCC14830.12725189955423222No Hit
GAGTGAATTCTGTCCCAGATCCACTGCCGCTGAACCTTGAGGGGACCCCA14690.12605060043504188No Hit
GAACTGAGGAGCAGGTGGGGGCACTTCTCCCTCTAACACTCTCCCCTGTT14350.12313315971700825No Hit
TTGCTGTCCTGCTCTGTGACACTCTCCTGGGAGTTACCCGATTGGAGGGC14110.12107378979839627No Hit
GACATGAGGGTCCCCGCTCAGCTCCTGGGGCTCCTGCTGCTCTGGCTCCC14000.12012991191903244No Hit
GTGCTGTCCTTGCTGTCCTGCTCTGTGACACTCTCCTGGGAGTTACCCGATTGGAGGGCGTTATCCACCTTCCA13940.11961506943937944No Hit
GCGTTATCCACCTTCCACTGTACTTTGGCCTCTCTGGGATAGAAGTTATTCAGCAGGCACACAACAGAGGCAGTT13910.11935764819955294No Hit
CTCCTGATCTATAAGACGTCTACTTTAGAAAGTGGGGTCCCCTCAAGGTT13810.11849957740013128No Hit
GCTCAGGCCCTGATGGGTGACTTCGCAGGCGTAGACTTTGTGTTTCTCGT13710.11764150660070961No Hit
GGATAGAAGTTATTCAGCAGGCACACAACAGAGGCAGTTCCAGATTTCAA13520.11601117208180846No Hit
ACTTTGGCCTCTCTGGGATAGAAGTTATTCAGCAGGCACACAACAGAGGC13240.11360857384342782No Hit
GTTCAGCGGCAGTGGATCTGGGACAGAATTCACTCTCATCATCAGCAGCC13140.11275050304400618No Hit
GTAATAAGTTGCAAAATCATCAGGCTGCAGGCTGCTGATGATGAGAGTGAATTCTGTCCCAGATCCACTGCCG13060.11206404640446883No Hit
GATCAGGAGTTTAGGGGCTTTCCCTGGTTTCTGCTGATACCAGGCCAACC12970.11129178268498933No Hit
CTTCTCCCTCTAACACTCTCCCCTGTTGAAGCTCTTTGTGACGGGCGAGC12960.11120597560504716No Hit
GATTTCAACTGCTCATCAGATGGCGGGAAGATGAAGACAGATGGTGCAGC12780.10966144816608818No Hit
GTGTCACAGAGCAGGACAGCAAGGACAGCACCTACAGCCTCAGCAGCACC12770.10957564108614601No Hit
CTGTGACACTCTCCTGGGAGTTACCCGATTGGAGGGCGTTATCCACCTTC12690.1088891844466087No Hit
GCTCTGTGACACTCTCCTGGGAGTTACCCGATTGGAGGGCGTTATCCACC12620.10828853488701352No Hit
GAATAACTTCTATCCCAGAGAGGCCAAAGTACAGTGGAAGGTGGATAACG12560.10777369240736054No Hit
GTCTCCGACAGATGCAGACAGGGTGGAAGGAGACTGGGTCATCTGGATGTCACATTTGGCACCTGGGAGCCAGAG12520.10743046408759187No Hit
CTGTAGCACTGTCTTCATCACAGATATTGCTCTGGGTAGCAGTAACTATC12460.10691562160793887No Hit
GTAATACATGGCGGTGTCCGAGGCCTTCAGGCTGGTCCACTGCAGGTAGG12220.10485625168932688No Hit
TTCTAAAGTAGACGTCTTATAGATCAGGAGTTTAGGGGCTTTCCCTGGTT11960.10262526761083057No Hit
AACTGTGGCTGCACCATCTGTCTTCATCTTCCCGCCATCTGATGAGCAGT11750.10082331893204509No Hit

[OK]Adapter Content

Adapter graph

[FAIL]Kmer Content

Kmer graph

SequenceCountPValueObs/Exp MaxMax Obs/Exp Position
CTTATAC4100.045.481461
CGTATGC3650.045.42321441
TCGTATG3500.045.33272640
TATCTCG3800.045.09308636
ATCTCGT3600.044.78317637
ACGAGAC4300.044.16020622
GTGGTAT2650.043.499941
GACCGAT4300.043.4238426
ACCGATC4350.042.9376427
ATCTCCG4300.042.5483410
CACGAGA4400.042.3804521
GTATCTC4100.041.74829535
CCGTCTT3800.041.33908547
GAGACCG4600.040.56394624
CGATCAG4650.040.21589729
AGACCGA4650.040.1467525
CCCACGA4700.039.66850719
TACACAT4700.039.6480835
CGAGACC4800.039.5686823
TCAGTAT4750.039.48484832
Produced by FastQC (version 0.11.5)