FastQCFastQC Report
Sat 23 Apr 2022
EGAF00004684415

Summary

[OK]Basic Statistics

MeasureValue
FilenameEGAF00004684415
File typeConventional base calls
EncodingSanger / Illumina 1.9
Total Sequences1165405
Sequences flagged as poor quality0
Sequence length20-76
%GC53

[OK]Per base sequence quality

Per base quality graph

[OK]Per tile sequence quality

Per base quality graph

[OK]Per sequence quality scores

Per Sequence quality graph

[FAIL]Per base sequence content

Per base sequence content

[FAIL]Per sequence GC content

Per sequence GC content graph

[OK]Per base N content

N content graph

[WARN]Sequence Length Distribution

Sequence length distribution

[FAIL]Sequence Duplication Levels

Duplication level graph

[WARN]Overrepresented sequences

SequenceCountPercentagePossible Source
CTCCTGGGAGTTACCCGATTGGAGGGCGTTATCCACCTTCCACTGTACTTTGGCCTCTCTGGGATAGAAGTTATT61720.5296012974030487No Hit
GTATAAAAGTTTGTACACTTTTGGCCCGGGGACCAAGCTGGAGATCAAACGAACTGTGGCTGCACCATCTGTCTT59010.5063475787387217No Hit
CTTTTATACTGTTGGCAGTAATAAGTTGCAAAATCATCAGGCTGCAGGCTGCTGATGATGAGAGTGAATTCTGTC52460.45014394137660296No Hit
CTCTAACACTCTCCCCTGTTGAAGCTCTTTGTGACGGGCGAGCTCAGGCC48190.41350431824129813No Hit
GGGGGGGGGGGGGGGGGGGGGGGGGGGGGGGGGGGGGGGGGGGGGGGGGG42380.36365040479489963No Hit
CTCCCAGGAGAGTGTCACAGAGCAGGACAGCAAGGACAGCACCTACAGCC40540.3478619020855411No Hit
GGCGTAGACTTTGTGTTTCTCGTAGTCTGCTTTGCTCAGCGTCAGGGTGC38100.3269249745796526No Hit
AGTCAGGACACAGCATGGACATGAGGGTCCCCGCTCAGCTCCTGGGGCTC37510.32186235686306475No Hit
ATTAGTAACTGGTTGGCCTGGTATCAGCAGAAACCAGGGAAAGCCCCTAA36030.3091629090316242No Hit
CTTTTGGCCCGGGGACCAAGCTGGAGATCAAACGAACTGTGGCTGCACCA34570.29663507536006795No Hit
GGCTGGAACTGAGGAGCAGGTGGGGGCACTTCTCCCTCTAACACTCTCCC33850.290456965604232No Hit
CAACAGAGGCAGTTCCAGATTTCAACTGCTCATCAGATGGCGGGAAGATG33030.2834207850489744No Hit
GTACAGTGGAAGGTGGATAACGCCCTCCAATCGGGTAACTCCCAGGAGAG32740.2809323797306516No Hit
GTCCTGCTCTGTGACACTCTCCTGGGAGTTACCCGATTGGAGGGCGTTATCCACCTTCCACTGTACTTTGGCCT32060.2750974982945843No Hit
GTCTACGCCTGCGAAGTCACCCATCAGGGCCTGAGCTCGCCCGTCACAAA31840.2732097425358566No Hit
GTCTGCATCTGTCGGAGACAGAGTCACCATCACTTGCCGGGCCAGTCAGA29850.2561341336273656No Hit
GTCTTCATCTTCCCGCCATCTGATGAGCAGTTGAAATCTGGAACTGCCTCTGTTGTGTGCCTGCTGAATAACTT29440.25261604334973675No Hit
GCGTCAGGGTGCTGCTGAGGCTGTAGGTGCTGTCCTTGCTGTCCTGCTCT29140.2500418309514718No Hit
CCTTCCACCCTGTCTGCATCTGTCGGAGACAGAGTCACCATCACTTGCCG28870.2477250397930333No Hit
GCATGGACATGAGGGTCCCCGCTCAGCTCCTGGGGCTCCTGCTGCTCTGG27290.23416752116217107No Hit
GACTATTAGTAACTGGTTGGCCTGGTATCAGCAGAAACCAGGGAAAGCCCCTAAACTCCTGATCTATAAGACGT27220.23356687160257594No Hit
GGATGGGAGGGGGTCAGGCTGGAACTGAGGAGCAGGTGGGGGCACTTCTCCCTCTAACACTCTCCCCTGTTGAAG27060.23219395832350126No Hit
CAATAGGGGTAGGTCCCCTGTGGAAAAAGGGTCAGAGGCCAAAGGATGGGAGGGGGTCAGGCTGGAACTGAGGAG26980.23150750168396392No Hit
GAATTCACTCTCATCATCAGCAGCCTGCAGCCTGATGATTTTGCAACTTA26130.22421389988887983No Hit
GGCCAAAAGTGTACAAACTTTTATACTGTTGGCAGTAATAAGTTGCAAAA25860.22189710873044133No Hit
GAGCAGGACAGCAAGGACAGCACCTACAGCCTCAGCAGCACCCTGACGCT25730.22078161669119317No Hit
GTGGCTGCACCATCTGTCTTCATCTTCCCGCCATCTGATGAGCAGTTGAAATCTGGAACTGCCTCTGTTGTGTGC25680.22035258129148236No Hit
CCACTGTACTTTGGCCTCTCTGGGATAGAAGTTATTCAGCAGGCACACAACAGAGGCAGTTCCAGATTTCAACTG24930.21391705029581992No Hit
CTCTGGGATAGAAGTTATTCAGCAGGCACACAACAGAGGCAGTTCCAGAT24680.21177187329726577No Hit
CAGCATGGACATGAGGGTCCCCGCTCAGCTCCTGGGGCTCCTGCTGCTCTGGCTCCCAGGTGCCAAATGTGACAT24270.20825378301963693No Hit
GGACACAGCATGGACATGAGGGTCCCCGCTCAGCTCCTGGGGCTCCTGCT23870.2048214998219503No Hit
GGCTGTAGGTGCTGTCCTTGCTGTCCTGCTCTGTGACACTCTCCTGGGAGTTACCCGATTGGAGGGCGTTATCCA23540.20198986618385883No Hit
GAGTGAATTCTGTCCCAGATCCACTGCCGCTGAACCTTGAGGGGACCCCA23390.2007027599847263No Hit
GTCGGAGACAGAGTCACCATCACTTGCCGGGCCAGTCAGACTATTAGTAACTGGTTGGCCTGGTATCAGCAGAAA23350.20035953166495768No Hit
CCCTGACGCTGAGCAAAGCAGACTACGAGAAACACAAAGTCTACGCCTGCGAAGTCACCCATCAGGGCCTGAGCT21870.1876600838335171No Hit
GACCCAGTCAGGACACAGCATGGACATGAGGGTCCCCGCTCAGCTCCTGG21520.18465683603554128No Hit
ACCTTGAGGGGACCCCACTTTCTAAAGTAGACGTCTTATAGATCAGGAGTTTAGGGGCTTTCCCTGGTTTCTG21000.18019486787854866No Hit
ATCCCAGAGAGGCCAAAGTACAGTGGAAGGTGGATAACGCCCTCCAATCG20850.1789077616794162No Hit
GCTCCTGGGGCTCCTGCTGCTCTGGCTCCCAGGTGCCAAATGTGACATCCAGATGACCCAGTCTCCTTCCACC20590.17667677760091985No Hit
GTTGTGTGCCTGCTGAATAACTTCTATCCCAGAGAGGCCAAAGTACAGTG19870.1704986678450839No Hit
CTAATAGTCTGACTGGCCCGGCAAGTGATGGTGACTCTGTCTCCGACAGA19800.16989801828548873No Hit
GTTACTAATAGTCTGACTGGCCCGGCAAGTGATGGTGACTCTGTCTCCGACAGATGCAGACAGGGTGGAAGGAGA19090.16380571560959495No Hit
CCTCAAGGTTCAGCGGCAGTGGATCTGGGACAGAATTCACTCTCATCATCAGCAGCCTGCAGCCTGATGATTTTG18730.16071666073167698No Hit
GGCCAAAGGATGGGAGGGGGTCAGGCTGGAACTGAGGAGCAGGTGGGGGCACTTCTCCCTCTAACACTCTCCC18560.15925794037266014No Hit
GTTTAGGGGCTTTCCCTGGTTTCTGCTGATACCAGGCCAACCAGTTACTA18430.15814244833341198No Hit
CCTACAGCCTCAGCAGCACCCTGACGCTGAGCAAAGCAGACTACGAGAAACACAAAGTCTACGCCTGCGAAGTCA18230.15642630673456867No Hit
GTCCTTGCTGTCCTGCTCTGTGACACTCTCCTGGGAGTTACCCGATTGGAGGGCGTTATCCACCTTCCACTGTA18150.15573985009503133No Hit
GGCCCTGATGGGTGACTTCGCAGGCGTAGACTTTGTGTTTCTCGTAGTCTGCTTTGCTCAGCGTCAGGGTGCTG18050.1548817792956097No Hit
GTACTTTGGCCTCTCTGGGATAGAAGTTATTCAGCAGGCACACAACAGAGGCAGTTCCAGATTTCAACTGCTCAT17980.15428112973601452No Hit
GCTTTGCTCAGCGTCAGGGTGCTGCTGAGGCTGTAGGTGCTGTCCTTGCT17800.15273660229705552No Hit
GTCTCCTTCCACCCTGTCTGCATCTGTCGGAGACAGAGTCACCATCACTTGCCGGGCCAGTCAGACTATTAGTAA17460.1498191615790219No Hit
GTTGAAATCTGGAACTGCCTCTGTTGTGTGCCTGCTGAATAACTTCTATC16810.14424170138278108No Hit
ACTTTAGAAAGTGGGGTCCCCTCAAGGTTCAGCGGCAGTGGATCTGGGACAGAATTCACTCTCATCATCAGCAGC16790.14407008722289677No Hit
CTTCTATCCCAGAGAGGCCAAAGTACAGTGGAAGGTGGATAACGCCCTCCAATCGGGTAACTCCCAGGAGAGTGT16780.14398428014295458No Hit
GTCAGGACACAGCATGGACATGAGGGTCCCCGCTCAGCTCCTGGGGCTCC16780.14398428014295458No Hit
CCTCTGTTGTGTGCCTGCTGAATAACTTCTATCCCAGAGAGGCCAAAGTA16330.14012296154555712No Hit
GTGGAAGGTGGATAACGCCCTCCAATCGGGTAACTCCCAGGAGAGTGTCACAGAGCAGGACAGCAAGGACAGCAC15900.136433257108044No Hit
GGGTGGAAGGAGACTGGGTCATCTGGATGTCACATTTGGCACCTGGGAGCCAGAGCAGCAGGAGCCCCAGGAG15880.13626164294815965No Hit
ACTTATTACTGCCAACAGTATAAAAGTTTGTACACTTTTGGCCCGGGGAC15570.1336016234699525No Hit
GTGTCACAGAGCAGGACAGCAAGGACAGCACCTACAGCCTCAGCAGCACC15510.1330867809902995No Hit
GAGCAAAGCAGACTACGAGAAACACAAAGTCTACGCCTGCGAAGTCACCC15110.12965449779261287No Hit
GTTGAAGCTCTTTGTGACGGGCGAGCTCAGGCCCTGATGGGTGACTTCGC14940.12819577743359603No Hit
ACCTTCCACTGTACTTTGGCCTCTCTGGGATAGAAGTTATTCAGCAGGCA14860.12750932079405872No Hit
GCCTCTGTTGTGTGCCTGCTGAATAACTTCTATCCCAGAGAGGCCAAAGTACAGTGGAAGGTGGATAACGCCCTC14820.12716609247429006No Hit
CCCCTAAACTCCTGATCTATAAGACGTCTACTTTAGAAAGTGGGGTCCCC14720.12630802167486838No Hit
CTCCTGATCTATAAGACGTCTACTTTAGAAAGTGGGGTCCCCTCAAGGTT14500.12442026591614073No Hit
GATGAAGACAGATGGTGCAGCCACAGTTCGTTTGATCTCCAGCTTGGTCC14350.12313315971700825No Hit
GTAATAAGTTGCAAAATCATCAGGCTGCAGGCTGCTGATGATGAGAGTGAATTCTGTCCCAGATCCACTGCCG14240.12218928183764444No Hit
CCCCTGTTGAAGCTCTTTGTGACGGGCGAGCTCAGGCCCTGATGGGTGACTTCGCAGGCGTAGACTTTGTGTTT14230.12210347475770227No Hit
GCACACAACAGAGGCAGTTCCAGATTTCAACTGCTCATCAGATGGCGGGA14100.1209879827184541No Hit
GTGCAGCCACAGTTCGTTTGATCTCCAGCTTGGTCCCCGGGCCAAAAGTG14060.12064475439868542No Hit
GTGCTGTCCTTGCTGTCCTGCTCTGTGACACTCTCCTGGGAGTTACCCGATTGGAGGGCGTTATCCACCTTCCA13830.11867119156001563No Hit
GTGGAAAAAGGGTCAGAGGCCAAAGGATGGGAGGGGGTCAGGCTGGAACTGAGGAGCAGGTGGGGGCACTTCTCC13820.11858538448007346No Hit
GGACATGAGGGTCCCCGCTCAGCTCCTGGGGCTCCTGCTGCTCTGGCTCC13700.11755569952076747No Hit
CATCATCAGCAGCCTGCAGCCTGATGATTTTGCAACTTATTACTGCCAACAGTATAAAAGTTTGTACACTTTTGG13660.1172124712009988No Hit
GCTCTGTGACACTCTCCTGGGAGTTACCCGATTGGAGGGCGTTATCCACC13580.11652601456146147No Hit
TTGCTGTCCTGCTCTGTGACACTCTCCTGGGAGTTACCCGATTGGAGGGC13430.11523890836232897No Hit
GGGTAGGTCCCCTGTGGAAAAAGGGTCAGAGGCCAAAGGATGGGAGGGGG13330.1143808375629073No Hit
GACATGAGGGTCCCCGCTCAGCTCCTGGGGCTCCTGCTGCTCTGGCTCCC13280.11395180216319649No Hit
GATTTCAACTGCTCATCAGATGGCGGGAAGATGAAGACAGATGGTGCAGC13040.1118924322445845No Hit
GGTATCAGCAGAAACCAGGGAAAGCCCCTAAACTCCTGATCTATAAGACGTCTACTTTAGAAAGTGGGGTCCCCT13030.11180662516464233No Hit
TTCTAAAGTAGACGTCTTATAGATCAGGAGTTTAGGGGCTTTCCCTGGTT12790.10974725524603035No Hit
ACTTTGGCCTCTCTGGGATAGAAGTTATTCAGCAGGCACACAACAGAGGC12710.10906079860649302No Hit
GTTCAGCGGCAGTGGATCTGGGACAGAATTCACTCTCATCATCAGCAGCC12680.10880337736666652No Hit
CTGTAGCACTGTCTTCATCACAGATATTGCTCTGGGTAGCAGTAACTATC12580.10794530656724485No Hit
GGATAGAAGTTATTCAGCAGGCACACAACAGAGGCAGTTCCAGATTTCAA12570.10785949948730271No Hit
GCGTTATCCACCTTCCACTGTACTTTGGCCTCTCTGGGATAGAAGTTATTCAGCAGGCACACAACAGAGGCAGTT12500.10725884992770754No Hit
GATCAGGAGTTTAGGGGCTTTCCCTGGTTTCTGCTGATACCAGGCCAACC12500.10725884992770754No Hit
CCGCAATAGGGGTAGGTCCCCTGTGGAAAAAGGGTCAGAGGCCAAAGGAT12490.10717304284776537No Hit
CTTTGTGACGGGCGAGCTCAGGCCCTGATGGGTGACTTCGCAGGCGTAGA12410.10648658620822804No Hit
CCTCCAACATTAGCATAATTAAAGCCAAGGAGGAGGAGGGGGGTGAGGTG12270.10528528708903771No Hit
GGTGAAAGATGAGCTGGAGGACCGCAATAGGGGTAGGTCCCCTGTGGAAA12200.10468463752944254No Hit
GAATAACTTCTATCCCAGAGAGGCCAAAGTACAGTGGAAGGTGGATAACG12180.10451302336955823No Hit
GCTCAGGCCCTGATGGGTGACTTCGCAGGCGTAGACTTTGTGTTTCTCGT12070.1035691454901944No Hit
CTATAAGACGTCTACTTTAGAAAGTGGGGTCCCCTCAAGGTTCAGCGGCAGTGGATCTGGGACAGAATTCACTCT12040.10331172425036789No Hit
GTCTTATAGATCAGGAGTTTAGGGGCTTTCCCTGGTTTCTGCTGATACCAGGCCAACCAGTTACTAATAGTCTGA11910.10219623221111974No Hit

[OK]Adapter Content

Adapter graph

[FAIL]Kmer Content

Kmer graph

SequenceCountPValueObs/Exp MaxMax Obs/Exp Position
GTATCAA25900.037.4888731
AATGGTA5450.034.55626370
TATCAAC29000.033.3361472
ATCAACG30150.032.0577433
TCAACGC30150.032.0577434
GTGGTAT2650.031.9164371
CAACGCA30550.031.6420735
AACGCAG31100.031.1911666
GATTCAC3000.030.448281
GCTTGTA902.3684879E-730.0723741
ACGCAGA33000.029.497737
CGCAGAG33450.029.2019468
AGAGTAC33300.029.02898611
GCAGAGT33800.028.799569
CGCATAA500.006339228728.74806459
CAGAGTA34000.028.5307410
ATAGGCG500.00820403627.26892532
GACAGCC1900.026.7090171
CGACCGG2700.026.28848811
TGCACCG2850.024.9048846
Produced by FastQC (version 0.11.5)