FastQCFastQC Report
Sat 23 Apr 2022
EGAF00004684418

Summary

[OK]Basic Statistics

MeasureValue
FilenameEGAF00004684418
File typeConventional base calls
EncodingSanger / Illumina 1.9
Total Sequences1024177
Sequences flagged as poor quality0
Sequence length20-76
%GC53

[OK]Per base sequence quality

Per base quality graph

[OK]Per tile sequence quality

Per base quality graph

[OK]Per sequence quality scores

Per Sequence quality graph

[FAIL]Per base sequence content

Per base sequence content

[FAIL]Per sequence GC content

Per sequence GC content graph

[OK]Per base N content

N content graph

[WARN]Sequence Length Distribution

Sequence length distribution

[FAIL]Sequence Duplication Levels

Duplication level graph

[WARN]Overrepresented sequences

SequenceCountPercentagePossible Source
CTCCTAAACTGCTCATTTACTGGGCGTCGACCCGGGAATCTGGGGTCCCT58650.5726549219519672No Hit
GTTTAGGAGACTGTCCTGCTTTCTGCTGATACCAAGCTAAGTAGTTCTTTCTGTTGGAACTGAATAAAACACT56120.5479521606128629No Hit
CTCCTGGGAGTTACCCGATTGGAGGGCGTTATCCACCTTCCACTGTACTTTGGCCTCTCTGGGATAGAAGTTATT47730.4660327267650025No Hit
CTCTAACACTCTCCCCTGTTGAAGCTCTTTGTGACGGGCGAGCTCAGGCC47350.4623224305954928No Hit
GGCGTAGACTTTGTGTTTCTCGTAGTCTGCTTTGCTCAGCGTCAGGGTGC32180.3142035019337478No Hit
GGCTGGAACTGAGGAGCAGGTGGGGGCACTTCTCCCTCTAACACTCTCCC30530.29809300540824485No Hit
CTCCCAGGAGAGTGTCACAGAGCAGGACAGCAAGGACAGCACCTACAGCC30340.29623785732349No Hit
CTATAATATTGCTGACAATAATAAAGTGCCACATCTTCAGCCTGCAGGCTGCTGATGGTGAGAGTGAAATCTGTC28710.2803226395437507No Hit
CAACAGAGGCAGTTCCAGATTTCAACTGCTCATCAGATGGCGGGAAGATG28500.27827221271323216No Hit
GTCTACGCCTGCGAAGTCACCCATCAGGGCCTGAGCTCGCCCGTCACAAA28260.2759288677640681No Hit
GTTTTATTCAGTTCCAACAGAAAGAACTACTTAGCTTGGTATCAGCAGAA27210.26567673361147537No Hit
GTACAGTGGAAGGTGGATAACGCCCTCCAATCGGGTAACTCCCAGGAGAG26600.2597207318656834No Hit
GGATGGGAGGGGGTCAGGCTGGAACTGAGGAGCAGGTGGGGGCACTTCTCCCTCTAACACTCTCCCCTGTTGAAG26530.25903725625551055No Hit
CAATAGGGGTAGGTCCCCTGTGGAAAAAGGGTCAGAGGCCAAAGGATGGGAGGGGGTCAGGCTGGAACTGAGGAG26520.2589396168826287No Hit
GTCCTGCTCTGTGACACTCTCCTGGGAGTTACCCGATTGGAGGGCGTTATCCACCTTCCACTGTACTTTGGCCT26230.2561080750690554No Hit
GCGTCAGGGTGCTGCTGAGGCTGTAGGTGCTGTCCTTGCTGTCCTGCTCT24770.24185272662830742No Hit
GTCTTCATCTTCCCGCCATCTGATGAGCAGTTGAAATCTGGAACTGCCTCTGTTGTGTGCCTGCTGAATAACTT23540.22984308376384158No Hit
GTGTTGCAGACCCAGGTCTTCATTTCTCTGTTGCTCTGGATCTCTGGTGG23130.22583986947568632No Hit
CTCTGGGATAGAAGTTATTCAGCAGGCACACAACAGAGGCAGTTCCAGAT20390.1990866813060633No Hit
CCACTGTACTTTGGCCTCTCTGGGATAGAAGTTATTCAGCAGGCACACAACAGAGGCAGTTCCAGATTTCAACTG20050.1957669426280809No Hit
ATATTATAGTAGTCCGCCGACGTTCGGCCAAGGGACCAGGGTGGAAATCAGACGAACTGTGGCTGCACCAT19510.19049441649246177No Hit
GTGGCTGCACCATCTGTCTTCATCTTCCCGCCATCTGATGAGCAGTTGAAATCTGGAACTGCCTCTGTTGTGTGC18990.1854171691026063No Hit
GGCTGTAGGTGCTGTCCTTGCTGTCCTGCTCTGTGACACTCTCCTGGGAGTTACCCGATTGGAGGGCGTTATCCA18940.18492897223819713No Hit
GGCCAAAGGATGGGAGGGGGTCAGGCTGGAACTGAGGAGCAGGTGGGGGCACTTCTCCCTCTAACACTCTCCC18890.18444077537378795No Hit
GCAGAATACCTGACTGCGTCTCTGGGCGAGAGGGCCACCATCAACTGCAG18560.18121867606868736No Hit
GAGCAGGACAGCAAGGACAGCACCTACAGCCTCAGCAGCACCCTGACGCT18100.1767272649161229No Hit
GAATAAAACACTCTGGCTGGACCTGCAGTTGATGGTGGCCCTCTCGCCCAGAGACGCAGTCAGGTATTCTGCAGA18030.17604378930595005No Hit
ATACCTGACTGCGTCTCTGGGCGAGAGGGCCACCATCAACTGCAGGTCCAGCCAGAGTGTTTTATTCAGTTCCAA17200.16793972135675767No Hit
ATCCCAGAGAGGCCAAAGTACAGTGGAAGGTGGATAACGCCCTCCAATCG16940.16540109766182992No Hit
AAGCAGGACAGTCTCCTAAACTGCTCATTTACTGGGCGTCGACCCGGGAA16890.16491290079742074No Hit
CTTATACACATCTCCGAGCCCACGAGACACTGAGCGATCTCGTATGCCGT16860.16461998267877526No Hit
GTCCTGCTTTCTGCTGATACCAAGCTAAGTAGTTCTTTCTGTTGGAACTG16540.16149552274655649No Hit
GCTTTGCTCAGCGTCAGGGTGCTGCTGAGGCTGTAGGTGCTGTCCTTGCT16280.15895689905162877No Hit
GTGCAGCCACAGTTCGTCTGATTTCCACCCTGGTCCCTTGGCCGAACGTC15830.15456312727194615No Hit
CAATAATAAAGTGCCACATCTTCAGCCTGCAGGCTGCTGATGGTGAGAGTGAAATCTGTCCCAGACCCGCTGCCA15740.15368437291600964No Hit
GGCCCTGATGGGTGACTTCGCAGGCGTAGACTTTGTGTTTCTCGTAGTCTGCTTTGCTCAGCGTCAGGGTGCTG15540.15173158545837293No Hit
GTTGTGTGCCTGCTGAATAACTTCTATCCCAGAGAGGCCAAAGTACAGTG15510.1514386673397274No Hit
GTTGAAGCTCTTTGTGACGGGCGAGCTCAGGCCCTGATGGGTGACTTCGC15010.14655669869563562No Hit
GGCAGGGGCAGCAAGATGGTGTTGCAGACCCAGGTCTTCATTTCTCTGTT14450.14108889381425282No Hit
GTTGAAATCTGGAACTGCCTCTGTTGTGTGCCTGCTGAATAACTTCTATC14280.1394290244752616No Hit
GATTTCACTCTCACCATCAGCAGCCTGCAGGCTGAAGATGTGGCACTTTATTATTGTCAGCAATATTATAGTAGT14250.13913610635661608No Hit
AACTGTGGCTGCACCATCTGTCTTCATCTTCCCGCCATCTGATGAGCAGT14180.13845263074644323No Hit
GTACTTTGGCCTCTCTGGGATAGAAGTTATTCAGCAGGCACACAACAGAGGCAGTTCCAGATTTCAACTGCTCAT13660.13337538335658777No Hit
GTCCTTGCTGTCCTGCTCTGTGACACTCTCCTGGGAGTTACCCGATTGGAGGGCGTTATCCACCTTCCACTGTA13610.1328871864921786No Hit
GAGCAAAGCAGACTACGAGAAACACAAAGTCTACGCCTGCGAAGTCACCC13490.13171551401759657No Hit
CTTCTATCCCAGAGAGGCCAAAGTACAGTGGAAGGTGGATAACGCCCTCCAATCGGGTAACTCCCAGGAGAGTGT13430.13112967778030554No Hit
GCTCATTTACTGGGCGTCGACCCGGGAATCTGGGGTCCCTGACCGATTCAGTGGCAGCGGGTCTGGGACAGATTT13340.13025092342436903No Hit
GTGGAAAAAGGGTCAGAGGCCAAAGGATGGGAGGGGGTCAGGCTGGAACTGAGGAGCAGGTGGGGGCACTTCTCC13160.128493414712496No Hit
GGGTGGAAATCAGACGAACTGTGGCTGCACCATCTGTCTTCATCTTCCCG13130.12820049659385047No Hit
CCTCTGTTGTGTGCCTGCTGAATAACTTCTATCCCAGAGAGGCCAAAGTA13120.12810285722096865No Hit
GTGGAAGGTGGATAACGCCCTCCAATCGGGTAACTCCCAGGAGAGTGTCACAGAGCAGGACAGCAAGGACAGCAC12980.12673590600062293No Hit
GTAAATGAGCAGTTTAGGAGACTGTCCTGCTTTCTGCTGATACCAAGCTA12910.12605243039045008No Hit
ACTATAATATTGCTGACAATAATAAAGTGCCACATCTTCAGCCTGCAGGCTGCTGATGGTGAGAGTGAAAT12880.12575951227180457No Hit
GAACTACTTAGCTTGGTATCAGCAGAAAGCAGGACAGTCTCCTAAACTGC12850.12546659415315908No Hit
GAATACCTGACTGCGTCTCTGGGCGAGAGGGCCACCATCAACTGCAGGTC12840.12536895478027724No Hit
CCCCTGTTGAAGCTCTTTGTGACGGGCGAGCTCAGGCCCTGATGGGTGACTTCGCAGGCGTAGACTTTGTGTTT12650.12351380669552235No Hit
ATTCAGTGGCAGCGGGTCTGGGACAGATTTCACTCTCACCATCAGCAGCC12640.12341616732264053No Hit
GGATCTCTGGTGGCTCCGGGGACATCGTGATGACCCAGTCTGCAGAATACCTGACTGCGTCTCTGGGCGAGAGGG12630.12331852794975869No Hit
GGGTAGGTCCCCTGTGGAAAAAGGGTCAGAGGCCAAAGGATGGGAGGGGG12610.123123249203995No Hit
GGTGAAAGATGAGCTGGAGGACCGCAATAGGGGTAGGTCCCCTGTGGAAA12440.12146337986500379No Hit
ACCTTCCACTGTACTTTGGCCTCTCTGGGATAGAAGTTATTCAGCAGGCA12250.11960823178024892No Hit
GCCTCTGTTGTGTGCCTGCTGAATAACTTCTATCCCAGAGAGGCCAAAGTACAGTGGAAGGTGGATAACGCCCTC12190.1190223955429579No Hit
CCTCCAACATTAGCATAATTAAAGCCAAGGAGGAGGAGGGGGGTGAGGTG12130.11843655930566688No Hit
GAGGAGGAGGGGGGTGAGGTGAAAGATGAGCTGGAGGACCGCAATAGGGG12090.11804600181413955No Hit
CTACTATAATATTGCTGACAATAATAAAGTGCCACATCTTCAGCCTGCAGGCTGCTGATGGTGAGAGTGAAAT12090.11804600181413955No Hit
GAACTGAGGAGCAGGTGGGGGCACTTCTCCCTCTAACACTCTCCCCTGTT11950.11667905059379385No Hit
CCGCAATAGGGGTAGGTCCCCTGTGGAAAAAGGGTCAGAGGCCAAAGGAT11890.11609321435650283No Hit
TTATTATTGTCAGCAATATTATAGTAGTCCGCCGACGTTCGGCCAAGGGA11490.1121876394412294No Hit
GCACACAACAGAGGCAGTTCCAGATTTCAACTGCTCATCAGATGGCGGGA11430.11160180320393838No Hit
GGACCAGGGTGGAAATCAGACGAACTGTGGCTGCACCATCTGTCTTCATC11420.11150416383105655No Hit
GCTCAGGCCCTGATGGGTGACTTCGCAGGCGTAGACTTTGTGTTTCTCGT11200.10935609762765615No Hit
GTGCTGTCCTTGCTGTCCTGCTCTGTGACACTCTCCTGGGAGTTACCCGATTGGAGGGCGTTATCCACCTTCCA11190.10925845825477433No Hit
CTCCGGGGACATCGTGATGACCCAGTCTGCAGAATACCTGACTGCGTCTCTGGGCGAGAGGGCCACCATCAACTG11180.1091608188818925No Hit
GTTCCAACAGAAAGAACTACTTAGCTTGGTATCAGCAGAAAGCAGGACAGTCTCCTAAACTGCTCATTTACTGGG11060.10798914640731046No Hit
GGATAGAAGTTATTCAGCAGGCACACAACAGAGGCAGTTCCAGATTTCAA10800.10545052271238271No Hit
GTCCCTGACCGATTCAGTGGCAGCGGGTCTGGGACAGATTTCACTCTCAC10750.10496232584797355No Hit
CTTTGTGACGGGCGAGCTCAGGCCCTGATGGGTGACTTCGCAGGCGTAGA10600.10349773525474601No Hit
GCGTTATCCACCTTCCACTGTACTTTGGCCTCTCTGGGATAGAAGTTATTCAGCAGGCACACAACAGAGGCAGTT10520.10271662027169133No Hit
TCTTCAGCCTGCAGGCTGCTGATGGTGAGAGTGAAATCTGTCCCAGACCCGCTGCCACTGAATCGGTCAGGGACC10520.10271662027169133No Hit
AGATGGTGTTGCAGACCCAGGTCTTCATTTCTCTGTTGCTCTGGATCTCTGGTGGCTCCGGGGACATCGTGATGA10440.10193550528863662No Hit
ACTTTGGCCTCTCTGGGATAGAAGTTATTCAGCAGGCACACAACAGAGGC10430.10183786591575479No Hit
TTGCTGTCCTGCTCTGTGACACTCTCCTGGGAGTTACCCGATTGGAGGGC10390.10144730842422744No Hit
GGTCCAGCCAGAGTGTTTTATTCAGTTCCAACAGAAAGAACTACTTAGCTTGGTATCAGCAGAAAGCAGGACAGT10340.10095911155981827No Hit
GATGAAGACAGATGGTGCAGCCACAGTTCGTCTGATTTCCACCCTGGTCC10330.10086147218693643No Hit
GTGCAGTGACAGTCGGGGGTGGCAGCATGAGGGAGATGGGGTAGGTGGAG10300.10056855406829093No Hit
GCCAAGGGACCAGGGTGGAAATCAGACGAACTGTGGCTGCACCATCTGTC10250.10008035720388175No Hit
GCTCTGTGACACTCTCCTGGGAGTTACCCGATTGGAGGGCGTTATCCACC10250.10008035720388175No Hit

[OK]Adapter Content

Adapter graph

[FAIL]Kmer Content

Kmer graph

SequenceCountPValueObs/Exp MaxMax Obs/Exp Position
CGTATGC2850.047.262841
TCGTATG2850.045.97994240
CGTGGAT306.920803E-445.14704517
ACGAGAC3050.044.43296422
CGAGACA3100.043.72271323
GTATGCC3200.042.17534642
CACGAGA3300.042.08733421
GTCGTAT758.913048E-1041.3100539
TGAGCGA3300.041.2623731
CTTATAC3300.041.062821
CCGTCTT3450.040.4960647
GAGCGAT3150.040.01859332
GCCGTCT3400.039.99983246
ATGCCGT3400.039.85553744
TCTCCGA3600.038.55933811
CCCACGA3650.038.04041319
ATCTCGT2450.037.778437
TTATACA3850.036.9402962
GAGTTTA2400.036.6998941
TATACAC3800.036.5352483
Produced by FastQC (version 0.11.5)