FastQCFastQC Report
Sat 23 Apr 2022
EGAF00004684419

Summary

[OK]Basic Statistics

MeasureValue
FilenameEGAF00004684419
File typeConventional base calls
EncodingSanger / Illumina 1.9
Total Sequences1024177
Sequences flagged as poor quality0
Sequence length20-76
%GC54

[OK]Per base sequence quality

Per base quality graph

[OK]Per tile sequence quality

Per base quality graph

[OK]Per sequence quality scores

Per Sequence quality graph

[FAIL]Per base sequence content

Per base sequence content

[FAIL]Per sequence GC content

Per sequence GC content graph

[OK]Per base N content

N content graph

[WARN]Sequence Length Distribution

Sequence length distribution

[FAIL]Sequence Duplication Levels

Duplication level graph

[WARN]Overrepresented sequences

SequenceCountPercentagePossible Source
CTCCTAAACTGCTCATTTACTGGGCGTCGACCCGGGAATCTGGGGTCCCT53190.5193438243584849No Hit
GTTTAGGAGACTGTCCTGCTTTCTGCTGATACCAAGCTAAGTAGTTCTTTCTGTTGGAACTGAATAAAACACT51680.5046002790533277No Hit
ATATTATAGTAGTCCGCCGACGTTCGGCCAAGGGACCAGGGTGGAAATCAGACGAACTGTGGCTGCACCAT49290.481264468934569No Hit
CTCCTGGGAGTTACCCGATTGGAGGGCGTTATCCACCTTCCACTGTACTTTGGCCTCTCTGGGATAGAAGTTATT42360.41360038352745665No Hit
GGGGGGGGGGGGGGGGGGGGGGGGGGGGGGGGGGGGGGGGGGGGGGGGGG40570.39612293578160807No Hit
CTCTAACACTCTCCCCTGTTGAAGCTCTTTGTGACGGGCGAGCTCAGGCC37150.3627302702560202No Hit
CTCCCAGGAGAGTGTCACAGAGCAGGACAGCAAGGACAGCACCTACAGCC31830.3107861238828835No Hit
CTATAATATTGCTGACAATAATAAAGTGCCACATCTTCAGCCTGCAGGCTGCTGATGGTGAGAGTGAAATCTGTC31590.30844277893371946No Hit
GGCGTAGACTTTGTGTTTCTCGTAGTCTGCTTTGCTCAGCGTCAGGGTGC29700.2899889374590525No Hit
GGCTGGAACTGAGGAGCAGGTGGGGGCACTTCTCCCTCTAACACTCTCCC26730.26099004371314727No Hit
GTACAGTGGAAGGTGGATAACGCCCTCCAATCGGGTAACTCCCAGGAGAG25560.24956623708597245No Hit
GTCTACGCCTGCGAAGTCACCCATCAGGGCCTGAGCTCGCCCGTCACAAA25290.24692997401816286No Hit
GTCTTCATCTTCCCGCCATCTGATGAGCAGTTGAAATCTGGAACTGCCTCTGTTGTGTGCCTGCTGAATAACTT23970.23404157679776053No Hit
GGATGGGAGGGGGTCAGGCTGGAACTGAGGAGCAGGTGGGGGCACTTCTCCCTCTAACACTCTCCCCTGTTGAAG22830.22291068828923125No Hit
GTCCTGCTCTGTGACACTCTCCTGGGAGTTACCCGATTGGAGGGCGTTATCCACCTTCCACTGTACTTTGGCCT22740.22203193393329473No Hit
GTTTTATTCAGTTCCAACAGAAAGAACTACTTAGCTTGGTATCAGCAGAA22570.22037206459430353No Hit
CAACAGAGGCAGTTCCAGATTTCAACTGCTCATCAGATGGCGGGAAGATG22040.21519717783156622No Hit
GTGTTGCAGACCCAGGTCTTCATTTCTCTGTTGCTCTGGATCTCTGGTGG21580.21070576667900176No Hit
GCGTCAGGGTGCTGCTGAGGCTGTAGGTGCTGTCCTTGCTGTCCTGCTCT20270.19791500883148128No Hit
TTATTATTGTCAGCAATATTATAGTAGTCCGCCGACGTTCGGCCAAGGGA20160.19684097572978107No Hit
GAGCAGGACAGCAAGGACAGCACCTACAGCCTCAGCAGCACCCTGACGCT20120.1964504182382537No Hit
CAATAATAAAGTGCCACATCTTCAGCCTGCAGGCTGCTGATGGTGAGAGTGAAATCTGTCCCAGACCCGCTGCCA20110.19635277886537192No Hit
CAATAGGGGTAGGTCCCCTGTGGAAAAAGGGTCAGAGGCCAAAGGATGGGAGGGGGTCAGGCTGGAACTGAGGAG19630.19166608896704376No Hit
GTGGCTGCACCATCTGTCTTCATCTTCCCGCCATCTGATGAGCAGTTGAAATCTGGAACTGCCTCTGTTGTGTGC18110.17682490428900474No Hit
GTCCCAGACCCGCTGCCACTGAATCGGTCAGGGACCCCAGATTCCCGGGT17700.17282169000084946No Hit
GGCTGTAGGTGCTGTCCTTGCTGTCCTGCTCTGTGACACTCTCCTGGGAGTTACCCGATTGGAGGGCGTTATCCA17590.17174765689914928No Hit
CCCTGACGCTGAGCAAAGCAGACTACGAGAAACACAAAGTCTACGCCTGCGAAGTCACCCATCAGGGCCTGAGCT17050.16647513076353013No Hit
ATACCTGACTGCGTCTCTGGGCGAGAGGGCCACCATCAACTGCAGGTCCAGCCAGAGTGTTTTATTCAGTTCCAA17030.16627985201776646No Hit
CCACTGTACTTTGGCCTCTCTGGGATAGAAGTTATTCAGCAGGCACACAACAGAGGCAGTTCCAGATTTCAACTG16990.1658892945262391No Hit
GAGTGAAATCTGTCCCAGACCCGCTGCCACTGAATCGGTCAGGGACCCCA16580.16188608023808385No Hit
GCAGAATACCTGACTGCGTCTCTGGGCGAGAGGGCCACCATCAACTGCAG16070.1569064722211102No Hit
CTCTGGGATAGAAGTTATTCAGCAGGCACACAACAGAGGCAGTTCCAGAT15890.15514896350923718No Hit
ATCCCAGAGAGGCCAAAGTACAGTGGAAGGTGGATAACGCCCTCCAATCG15610.15241506106854577No Hit
TCTTCAGCCTGCAGGCTGCTGATGGTGAGAGTGAAATCTGTCCCAGACCCGCTGCCACTGAATCGGTCAGGGACC15120.14763073179733582No Hit
GGGTGGAAATCAGACGAACTGTGGCTGCACCATCTGTCTTCATCTTCCCG15050.14694725618716295No Hit
GATTTCACTCTCACCATCAGCAGCCTGCAGGCTGAAGATGTGGCACTTTATTATTGTCAGCAATATTATAGTAGT15030.14675197744139928No Hit
GTCCTGCTTTCTGCTGATACCAAGCTAAGTAGTTCTTTCTGTTGGAACTG15010.14655669869563562No Hit
AAGCAGGACAGTCTCCTAAACTGCTCATTTACTGGGCGTCGACCCGGGAA14980.1462637805769901No Hit
GTTGTGTGCCTGCTGAATAACTTCTATCCCAGAGAGGCCAAAGTACAGTG14910.14558030496681726No Hit
GGCCAAAGGATGGGAGGGGGTCAGGCTGGAACTGAGGAGCAGGTGGGGGCACTTCTCCCTCTAACACTCTCCC14750.1440180750007079No Hit
GTGCAGCCACAGTTCGTCTGATTTCCACCCTGGTCCCTTGGCCGAACGTC14730.1438227962549442No Hit
ACTATAATATTGCTGACAATAATAAAGTGCCACATCTTCAGCCTGCAGGCTGCTGATGGTGAGAGTGAAAT14680.14333459939053503No Hit
AACTGTGGCTGCACCATCTGTCTTCATCTTCCCGCCATCTGATGAGCAGT14160.13825735200067957No Hit
CCTACAGCCTCAGCAGCACCCTGACGCTGAGCAAAGCAGACTACGAGAAACACAAAGTCTACGCCTGCGAAGTCA14020.13689040078033388No Hit
CTACTATAATATTGCTGACAATAATAAAGTGCCACATCTTCAGCCTGCAGGCTGCTGATGGTGAGAGTGAAAT13980.13649984328880654No Hit
GGCAGGGGCAGCAAGATGGTGTTGCAGACCCAGGTCTTCATTTCTCTGTT13800.13474233457693346No Hit
TTATAGTAGTCCGCCGACGTTCGGCCAAGGGACCAGGGTGGAAATCAGACGAACTGTGGCTGCACCATCTGTCTT13610.1328871864921786No Hit
CTTCTATCCCAGAGAGGCCAAAGTACAGTGGAAGGTGGATAACGCCCTCCAATCGGGTAACTCCCAGGAGAGTGT13450.13132495652606924No Hit
GGCCCTGATGGGTGACTTCGCAGGCGTAGACTTTGTGTTTCTCGTAGTCTGCTTTGCTCAGCGTCAGGGTGCTG13390.1307391202887782No Hit
GAATAAAACACTCTGGCTGGACCTGCAGTTGATGGTGGCCCTCTCGCCCAGAGACGCAGTCAGGTATTCTGCAGA13060.12751702098367762No Hit
GTTGAAATCTGGAACTGCCTCTGTTGTGTGCCTGCTGAATAACTTCTATC13020.1271264634921503No Hit
GTACTTTGGCCTCTCTGGGATAGAAGTTATTCAGCAGGCACACAACAGAGGCAGTTCCAGATTTCAACTGCTCAT12780.12478311854298622No Hit
GCTTTGCTCAGCGTCAGGGTGCTGCTGAGGCTGTAGGTGCTGTCCTTGCT12690.12390436418704971No Hit
CCTCTGTTGTGTGCCTGCTGAATAACTTCTATCCCAGAGAGGCCAAAGTA12660.12361144606840418No Hit
GTCCTTGCTGTCCTGCTCTGTGACACTCTCCTGGGAGTTACCCGATTGGAGGGCGTTATCCACCTTCCACTGTA12370.12077990425483096No Hit
GTGTCACAGAGCAGGACAGCAAGGACAGCACCTACAGCCTCAGCAGCACC12190.1190223955429579No Hit
GAGCAAAGCAGACTACGAGAAACACAAAGTCTACGCCTGCGAAGTCACCC12090.11804600181413955No Hit
GGATCTCTGGTGGCTCCGGGGACATCGTGATGACCCAGTCTGCAGAATACCTGACTGCGTCTCTGGGCGAGAGGG11910.11628849310226651No Hit
GTGGAAGGTGGATAACGCCCTCCAATCGGGTAACTCCCAGGAGAGTGTCACAGAGCAGGACAGCAAGGACAGCAC11830.11550737811921181No Hit
GCCTCTGTTGTGTGCCTGCTGAATAACTTCTATCCCAGAGAGGCCAAAGTACAGTGGAAGGTGGATAACGCCCTC11610.11335931191581144No Hit
GTATCAACGCAGAGTACGGGGAGCTACAACAGGCAGGCAGGGGCAGCAAG11570.11296875442428408No Hit
ATTCAGTGGCAGCGGGTCTGGGACAGATTTCACTCTCACCATCAGCAGCC11440.11169944257682023No Hit
GCTCATTTACTGGGCGTCGACCCGGGAATCTGGGGTCCCTGACCGATTCAGTGGCAGCGGGTCTGGGACAGATTT11330.11062540947512002No Hit
GAATACCTGACTGCGTCTCTGGGCGAGAGGGCCACCATCAACTGCAGGTC11260.10994193386494716No Hit
CCCCTGTTGAAGCTCTTTGTGACGGGCGAGCTCAGGCCCTGATGGGTGACTTCGCAGGCGTAGACTTTGTGTTT11180.1091608188818925No Hit
CACCATCAGCAGCCTGCAGGCTGAAGATGTGGCACTTTATTATTGTCAGCAATATTATAGTAGTCCGCCGACGTT11120.10857498264460147No Hit
GGCTGAAGATGTGGCACTTTATTATTGTCAGCAATATTATAGTAGTCCGC11070.10808678578019229No Hit
GTTGAAGCTCTTTGTGACGGGCGAGCTCAGGCCCTGATGGGTGACTTCGC10930.1067198345598466No Hit
CTCCGGGGACATCGTGATGACCCAGTCTGCAGAATACCTGACTGCGTCTCTGGGCGAGAGGGCCACCATCAACTG10930.1067198345598466No Hit
GTGGAAAAAGGGTCAGAGGCCAAAGGATGGGAGGGGGTCAGGCTGGAACTGAGGAGCAGGTGGGGGCACTTCTCC10860.10603635894967374No Hit
GTAAATGAGCAGTTTAGGAGACTGTCCTGCTTTCTGCTGATACCAAGCTA10820.1056458014581464No Hit
GGACCAGGGTGGAAATCAGACGAACTGTGGCTGCACCATCTGTCTTCATC10680.10427885023780069No Hit
GTCCCTGACCGATTCAGTGGCAGCGGGTCTGGGACAGATTTCACTCTCAC10540.10291189901745498No Hit
ACCTTCCACTGTACTTTGGCCTCTCTGGGATAGAAGTTATTCAGCAGGCA10540.10291189901745498No Hit
GAACTACTTAGCTTGGTATCAGCAGAAAGCAGGACAGTCTCCTAAACTGC10380.10134966905134561No Hit
GCAAGATGGTGTTGCAGACCCAGGTCTTCATTTCTCTGTTGCTCTGGATC10270.10027563594964542No Hit

[OK]Adapter Content

Adapter graph

[WARN]Kmer Content

Kmer graph

SequenceCountPValueObs/Exp MaxMax Obs/Exp Position
TTACGAA200.008017573550.6397174
GAAACTA606.935531E-445.12323870
GTGGTAT2200.044.547251
GTATCAA23100.037.3055761
TATCAAC25400.034.028962
AATGGTA3400.033.84242670
ACGTGAT800.002851747433.84242670
ATACGTA808.5789E-833.76146311
ATCAACG26650.032.4296883
TCAACGC26900.032.12834
TACGTAC851.4609031E-731.77549412
GGGTTAG751.9753443E-631.5122367
GGTCTGG22150.031.47422270
AACGCAG27400.031.4218736
CAACGCA27450.031.3631065
ACGTCAC450.004591392430.70890241
CGTTTAT450.004905966630.29567733
ACGCAGA28450.030.262197
CGCAGAG28550.030.154728
GTCGTTG450.005113971430.0395221
Produced by FastQC (version 0.11.5)