FastQCFastQC Report
Fri 2 Sep 2022
EGAF00004684420

Summary

[OK]Basic Statistics

MeasureValue
FilenameEGAF00004684420
File typeConventional base calls
EncodingSanger / Illumina 1.9
Total Sequences598948
Sequences flagged as poor quality0
Sequence length20-76
%GC54

[OK]Per base sequence quality

Per base quality graph

[OK]Per tile sequence quality

Per tile quality graph

[OK]Per sequence quality scores

Per Sequence quality graph

[FAIL]Per base sequence content

Per base sequence content

[FAIL]Per sequence GC content

Per sequence GC content graph

[OK]Per base N content

N content graph

[WARN]Sequence Length Distribution

Sequence length distribution

[FAIL]Sequence Duplication Levels

Duplication level graph

[WARN]Overrepresented sequences

SequenceCountPercentagePossible Source
CTCCTGGGAGTTACCCGATTGGAGGGCGTTATCCACCTTCCACTGTACTTTGGCCTCTCTGGGATAGAAGTTATT17280.28850584691826336No Hit
CTCTAACACTCTCCCCTGTTGAAGCTCTTTGTGACGGGCGAGCTCAGGCC16850.2813265926257371No Hit
ATAATACACAGCCGTGTCTTCAATTCTCAGGCTGTTCATTTGCAGATAGA15460.2581192357266407No Hit
GTGCAGTGACAGTCGGGGGTGGCAGCATGAGGGAGATGGGGTAGGTGGAG14720.24576423996740954No Hit
GGGCTACAGTGGGTGGCAGTTGTATCATTTGATGGAAGAAATAAAGACTA14370.2399206608920975No Hit
CAATAATACACAGCCGTGTCTTCAATTCTCAGGCTGTTCATTTGCAGATA12760.21304019714566205No Hit
CTCCTGTGCAGCGTCTGGATTCACATTCAGTAGTTACTCTATGCACTGGG12190.203523511223011No Hit
GTCACATCCTGGCTGGGATTCGTGTAGTGCTTCACGTGGCATGTCACGGA12060.20135303899503795No Hit
GTCACTGCACCGACCGGCCCTCGAGGACCTGCTCTTAGGTTCAGAAGCGAACCTCACGTGCACACTGACCGGC11450.19116851546377983No Hit
GTATTATTGTGCGAGAGATAAAGAGGTTGTCTCCAAGAACTGGTTCGATCCTTGGGGACAGGGAACCCAGGTCAC11450.19116851546377983No Hit
GTGTACAGGTCCCCGGAGGCATCCTGGCTGGGTGGGAAGTTTCTGGCGGT10850.18115095133467346No Hit
AGGTCACACTGAGTGGCTCCTGGGGGAAGAAGCCCTGGACCAGGCAGGCG10850.18115095133467346No Hit
GTAGGAGACAGAGTCACCATCACTTGTCGGGCGAGTCACGATATTAAGAACTGGTTAGCCTGGTATCAGCAGAAA10680.1783126414980933No Hit
TCCTTGGGGCTGAAGCCACGTGCCAGGCACGTCAGCGTCACCAGCTCGTT10480.17497345345505788No Hit
GTATGCTGGTCACAGCGAAGGTGGTGGTGCCCTGGCTGGGCTCCTGCCGG10380.17330385943354013No Hit
GGCTGGAACTGAGGAGCAGGTGGGGGCACTTCTCCCTCTAACACTCTCCC10310.17213514361847773No Hit
GTGTATTATTGTGCGAGAGATAAAGAGGTTGTCTCCAAGAACTGGTTCGA10180.16996467139050467No Hit
AGTCAGGACACAGCATGGACATGAGGGTCCCCGCTCAGCTCCTGGGGCTC9900.16528980813025504No Hit
CAATAGGGGTAGGTCCCCTGTGGAAAAAGGGTCAGAGGCCAAAGGATGGGAGGGGGTCAGGCTGGAACTGAGGAG9830.16412109231519265No Hit
CAACAGAGGCAGTTCCAGATTTCAACTGCTCATCAGATGGCGGGAAGATG9810.1637871735108891No Hit
GCACTGAACACAGAGGACTCACCATGGAGTTTGGGCTGAGCTGGGTTTTC9790.16345325470658556No Hit
GTAGTTACTCTATGCACTGGGTCCGACAGGCTCCAGGCAAGGGGCTACAG9470.15811055383772882No Hit
CTGTTAGCCTGTTGACAAAAGTAAGTTGCAAAATCTTCAGGCTGCAGGCTGCTGATGGTGAGAGTGAAATCTGTC9450.15777663503342526No Hit
GGCTAACAGTTTCCCGCTCTCTTTCGGCGGAGGGGCCAAGGTGGAGATCAAACGAACTGTGGCTGCACCAT9430.15744271622912173No Hit
GTTCAAGGACCACCTGAGCGTGACCTCTGTGGCTGCTACAGCGTGTCCAG9370.1564409598162111No Hit
CTCCCAGGAGAGTGTCACAGAGCAGGACAGCAAGGACAGCACCTACAGCC9350.15610704101190756No Hit
GCTCAGTAGCAGGTGCCGTCCACCTCCGCCATGACAACAGACACATTGAC9300.15527224400114867No Hit
ATTAAGAACTGGTTAGCCTGGTATCAGCAGAAACCAGGTAAAGCCCCTAGACTCTTGATCTCTGGTGGATCCAC9120.15226697476241677No Hit
GTGTGCACGTGAGGTTCGCTTCTGAACCTAAGAGCAGGTCCTCGAGGGCC8950.14942866492583662No Hit
GTCTTGGACTCGGGGTAGGCAGCAGTGCAAGTGAAGGTCTTCCCATGGTT8930.1490947461215331No Hit
GTCCTGCTCTGTGACACTCTCCTGGGAGTTACCCGATTGGAGGGCGTTATCCACCTTCCACTGTACTTTGGCCT8890.148426908512926No Hit
GCACTGGAAGTCGCCGGTGTTTCCATTCGGTGATCAGCACTGAACACAGA8840.14759211150216714No Hit
CTCTATGCACTGGGTCCGACAGGCTCCAGGCAAGGGGCTACAGTGGGTGG8760.14625643628495294No Hit
GTGTGACCTGGAGCGAAAGCGGACAGGGCGTGACCGCCAGAAACTTCCCACCCAGCCAGGATGCCTCCGGGGAC8660.14458684226343524No Hit
GCATGGACATGAGGGTCCCCGCTCAGCTCCTGGGGCTCCTGCTGCTCTGG8570.14308420764406926No Hit
GGATGTGACTGTGCCCTGCCCAGTTCCCTCAACTCCACCTACCCCATCTCCCTCAACTCCACCTACCCCATCTCC8430.14074677601394445No Hit
GAGTTTATTCAGGGGTGGGGACAGGCGGGCGGCTCAGTAGCAGGTGCCGT8400.14024589780748914No Hit
GGCGTAGAGTTTGTGTTTCTCGTAGTCTGCTTTGCTCAGCGTCAGGGTGC8250.13774150677521255No Hit
GTGCCTAGCCGGCAAGTCCGTGACATGCCACGTGAAGCACTACACGAATCCCAGCCAGGATGTGACTGTGCCCTG7970.13306664351496292No Hit
GCCCTGGACCAGGCAGGCGATGACCACGTTCCCATCTGGCTGGGTGCTGC7840.13089617128698985No Hit
CCTTGGGGCTGGTCGGGGATGCTGAGGAGACGGTGACCTGGGTTCCCTGT7840.13089617128698985No Hit
GGATGGGAGGGGGTCAGGCTGGAACTGAGGAGCAGGTGGGGGCACTTCTCCCTCTAACACTCTCCCCTGTTGAAG7830.13072921188483808No Hit
GCTAGGCACTGTGTGGCCGGCAGGGTCAGCTGGCTGCTCGTGGTGTACAG7820.13056225248268633No Hit
GTCCAGGGCTTCTTCCCCCAGGAGCCACTCAGTGTGACCTGGAGCGAAAGCGGACAGGGCGTGACCGCCAGAAA7800.13022833367838277No Hit
GAGTGGCTCCTGGGGGAAGAAGCCCTGGACCAGGCAGGCGATGACCACGT7750.1293935366676239No Hit
GCGTCAGGGTGCTGCTGAGGCTGTAGGTGCTGTCCTTGCTGTCCTGCTCT7740.12922657726547213No Hit
CAGTATGCTGGTCACAGCGAAGGTGGTGGTGCCCTGGCTGGGCTCCTGCC7610.1270561050374991No Hit
CGTGCACACTGACCGGCCTGAGAGATGCCTCAGGTGTCACCTTCACCTGGACGCCCTCAAGTGGGAAGAGCG7490.1250525922116778No Hit
GTGGCTGCACCATCTGTCTTCATCTTCCCGCCATCTGATGAGCAGTTGAAATCTGGAACTGCCTCTGTTGTGTGC7460.12455171400522248No Hit
GTCTTCATCTTCCCGCCATCTGATGAGCAGTTGAAATCTGGAACTGCCTCTGTTGTGTGCCTGCTGAATAACTT7260.12121252596218703No Hit
GTGCAGCCACAGTTCGTTTGATCTCCACCTTGGCCCCTCCGCCGAAAGAG7260.12121252596218703No Hit
GTACAGTGGAAGGTGGATAACGCCCTCCAATCGGGTAACTCCCAGGAGAG7170.11970989134282109No Hit
GTGGAGTCCCATCAAGATTCAGCGGCAGTGGATCTGGGACAGATTTCACT7140.11920901313636575No Hit
GCCCCAAGGTCTTCCCGCTGAGCCTCTGCAGCACCCAGCCAGATGGGAAC7140.11920901313636575No Hit
GTACAGGTCCCCGGAGGCATCCTGGCTGGGTGGGAAGTTTCTGGCGGTCACGCCCTGTCCGCTTTCGCTCCAGGT7040.11753941911484803No Hit
CAGCATGGACATGAGGGTCCCCGCTCAGCTCCTGGGGCTCCTGCTGCTCTGGTTCCCAGGTTCCAGATGCGACAT7020.1172055003105445No Hit
CTCTGGGATAGAAGTTATTCAGCAGGCACACAACAGAGGCAGTTCCAGAT6980.1165376627019374No Hit
GATCAGCACTGAACACAGAGGACTCACCATGGAGTTTGGGCTGAGCTGGG6940.11586982509333031No Hit
CTTATACACATCTCCGAGCCCACGAGACGTAGAGGAATCTCGTATGCCGT6940.11586982509333031No Hit
CCCTGAGACTCTCCTGTGCAGCGTCTGGATTCACATTCAGTAGTTACTCT6640.11086104302877713No Hit
GTATCAACGCAGAGTACGGGAGCTCTGGGAGAGGAGCCCAGCACTGGAAG6600.11019320542017003No Hit
GATGAAGACAGATGGTGCAGCCACAGTTCGTTTGATCTCCACCTTGGCCCCTCCGCCGAAAGAGAGCGGGAAA6550.10935840840941118No Hit
CTGTAGCCCCTTGCCTGGAGCCTGTCGGACCCAGTGCATAGAGTAACTAC6550.10935840840941118No Hit
GTGCTGCAGAGGCTCAGCGGGAAGACCTTGGGGCTGGTCGGGGATGCTGAGGAGACGGTGACCTGGGTTCC6530.10902448960510762No Hit
GGGTCCGACAGGCTCCAGGCAAGGGGCTACAGTGGGTGGCAGTTGTATCATTTGATGGAAGAAATAAAGACTACG6450.10768881438789343No Hit
ATCCCAGAGAGGCCAAAGTACAGTGGAAGGTGGATAACGCCCTCCAATCG6310.10535138275776862No Hit
CCACTGTACTTTGGCCTCTCTGGGATAGAAGTTATTCAGCAGGCACACAACAGAGGCAGTTCCAGATTTCAACTG6290.10501746395346508No Hit
ATTCAGGGGTGGGGACAGGCGGGCGGCTCAGTAGCAGGTGCCGTCCACCT6150.10268003232334025No Hit
GTAGCAGGTGCCGTCCACCTCCGCCATGACAACAGACACATTGACATGGG6090.10167827591042963No Hit
ATGCAGGAGAAGGTGTCCCCCTTCTTCCAGTCCTCGGCTGCCACGCGCAG6070.10134435710612608No Hit
GTCACGGACTTGCCGGCTAGGCACTGTGTGGCCGGCAGGGTCAGCTGGCT6030.10067651949751898No Hit
CTCTACGCCTGCGAAGTCACCCATCAGGGCCTGAGCTCGCCCGTCACAAA5990.10000868188891189No Hit

[OK]Adapter Content

Adapter graph

Produced by FastQC (version 0.11.9)