FastQCFastQC Report
Wed 27 Apr 2022
EGAF00004684421

Summary

[OK]Basic Statistics

MeasureValue
FilenameEGAF00004684421
File typeConventional base calls
EncodingSanger / Illumina 1.9
Total Sequences598948
Sequences flagged as poor quality0
Sequence length20-76
%GC55

[OK]Per base sequence quality

Per base quality graph

[OK]Per tile sequence quality

Per tile quality graph

[OK]Per sequence quality scores

Per Sequence quality graph

[FAIL]Per base sequence content

Per base sequence content

[FAIL]Per sequence GC content

Per sequence GC content graph

[OK]Per base N content

N content graph

[WARN]Sequence Length Distribution

Sequence length distribution

[FAIL]Sequence Duplication Levels

Duplication level graph

[WARN]Overrepresented sequences

SequenceCountPercentagePossible Source
CTCCTGGGAGTTACCCGATTGGAGGGCGTTATCCACCTTCCACTGTACTTTGGCCTCTCTGGGATAGAAGTTATT16860.2814935520278889No Hit
GGGGGGGGGGGGGGGGGGGGGGGGGGGGGGGGGGGGGGGGGGGGGGGGGG15640.26112450496537265No Hit
ATAATACACAGCCGTGTCTTCAATTCTCAGGCTGTTCATTTGCAGATAGA15280.2551139664879088No Hit
CTCTAACACTCTCCCCTGTTGAAGCTCTTTGTGACGGGCGAGCTCAGGCC13740.2294022185565358No Hit
GGGCTACAGTGGGTGGCAGTTGTATCATTTGATGGAAGAAATAAAGACTA12700.2120384407327514No Hit
CAATAATACACAGCCGTGTCTTCAATTCTCAGGCTGTTCATTTGCAGATA11860.1980138509520025No Hit
GTATTATTGTGCGAGAGATAAAGAGGTTGTCTCCAAGAACTGGTTCGATCCTTGGGGACAGGGAACCCAGGTCAC11800.19701209453909188No Hit
CTGTAGCCCCTTGCCTGGAGCCTGTCGGACCCAGTGCATAGAGTAACTAC11210.1871614898121373No Hit
GTTCAAGGACCACCTGAGCGTGACCTCTGTGGCTGCTACAGCGTGTCCAG11200.1869945304099855No Hit
CTCCCAGGAGAGTGTCACAGAGCAGGACAGCAAGGACAGCACCTACAGCC11160.18632669280137842No Hit
CTCCTGTGCAGCGTCTGGATTCACATTCAGTAGTTACTCTATGCACTGGG11040.18432317997555714No Hit
GTGCAGTGACAGTCGGGGGTGGCAGCATGAGGGAGATGGGGTAGGTGGAG10550.17614216927012027No Hit
GTGTGACCTGGAGCGAAAGCGGACAGGGCGTGACCGCCAGAAACTTCCCACCCAGCCAGGATGCCTCCGGGGAC10350.17280298122708482No Hit
GTGTACAGGTCCCCGGAGGCATCCTGGCTGGGTGGGAAGTTTCTGGCGGT10320.1723021030206295No Hit
GTGTATTATTGTGCGAGAGATAAAGAGGTTGTCTCCAAGAACTGGTTCGA10260.17130034660771887No Hit
GTCACTGCACCGACCGGCCCTCGAGGACCTGCTCTTAGGTTCAGAAGCGAACCTCACGTGCACACTGACCGGC9680.16161670128291605No Hit
AGGTCACACTGAGTGGCTCCTGGGGGAAGAAGCCCTGGACCAGGCAGGCG9600.16028102606570185No Hit
AGTCAGGACACAGCATGGACATGAGGGTCCCCGCTCAGCTCCTGGGGCTC9530.15911231025063946No Hit
GTAGTTACTCTATGCACTGGGTCCGACAGGCTCCAGGCAAGGGGCTACAG9510.1587783914463359No Hit
GTCACATCCTGGCTGGGATTCGTGTAGTGCTTCACGTGGCATGTCACGGA9380.15660791921836287No Hit
GTCCTGCTCTGTGACACTCTCCTGGGAGTTACCCGATTGGAGGGCGTTATCCACCTTCCACTGTACTTTGGCCT9110.152100015360265No Hit
CTGTTAGCCTGTTGACAAAAGTAAGTTGCAAAATCTTCAGGCTGCAGGCTGCTGATGGTGAGAGTGAAATCTGTC9050.15109825894735437No Hit
GTAGGAGACAGAGTCACCATCACTTGTCGGGCGAGTCACGATATTAAGAACTGGTTAGCCTGGTATCAGCAGAAA8910.14876082731722953No Hit
GGCTAACAGTTTCCCGCTCTCTTTCGGCGGAGGGGCCAAGGTGGAGATCAAACGAACTGTGGCTGCACCAT8880.14825994911077423No Hit
ATTAAGAACTGGTTAGCCTGGTATCAGCAGAAACCAGGTAAAGCCCCTAGACTCTTGATCTCTGGTGGATCCAC8850.14775907090431892No Hit
GGCTGGAACTGAGGAGCAGGTGGGGGCACTTCTCCCTCTAACACTCTCCC8610.14375204525267635No Hit
GTCCAGGGCTTCTTCCCCCAGGAGCCACTCAGTGTGACCTGGAGCGAAAGCGGACAGGGCGTGACCGCCAGAAA8430.14074677601394445No Hit
CAACAGAGGCAGTTCCAGATTTCAACTGCTCATCAGATGGCGGGAAGATG8360.13957806019888205No Hit
CGTGCACACTGACCGGCCTGAGAGATGCCTCAGGTGTCACCTTCACCTGGACGCCCTCAAGTGGGAAGAGCG8230.137407587970909No Hit
CTCTATGCACTGGGTCCGACAGGCTCCAGGCAAGGGGCTACAGTGGGTGG8070.13473623753648062No Hit
GCCCCAAGGTCTTCCCGCTGAGCCTCTGCAGCACCCAGCCAGATGGGAAC7870.13139704949344516No Hit
GTACAGTGGAAGGTGGATAACGCCCTCCAATCGGGTAACTCCCAGGAGAG7740.12922657726547213No Hit
GGATGTGACTGTGCCCTGCCCAGTTCCCTCAACTCCACCTACCCCATCTCCCTCAACTCCACCTACCCCATCTCC7690.12839178025471326No Hit
GGCGTAGAGTTTGTGTTTCTCGTAGTCTGCTTTGCTCAGCGTCAGGGTGC7560.12622130802674023No Hit
CAATAGGGGTAGGTCCCCTGTGGAAAAAGGGTCAGAGGCCAAAGGATGGGAGGGGGTCAGGCTGGAACTGAGGAG7400.12354995759231185No Hit
CTCTGGGATAGAAGTTATTCAGCAGGCACACAACAGAGGCAGTTCCAGAT7370.12304907938585653No Hit
GTGCCTAGCCGGCAAGTCCGTGACATGCCACGTGAAGCACTACACGAATCCCAGCCAGGATGTGACTGTGCCCTG7320.12221428237509768No Hit
GCACTGAACACAGAGGACTCACCATGGAGTTTGGGCTGAGCTGGGTTTTC7320.12221428237509768No Hit
TCCTTGGGGCTGAAGCCACGTGCCAGGCACGTCAGCGTCACCAGCTCGTT7280.12154644476649058No Hit
CCTTGGGGCTGGTCGGGGATGCTGAGGAGACGGTGACCTGGGTTCCCTGT7250.12104556656003526No Hit
GTCTTGGACTCGGGGTAGGCAGCAGTGCAAGTGAAGGTCTTCCCATGGTT7230.1207116477557317No Hit
GCGTCAGGGTGCTGCTGAGGCTGTAGGTGCTGTCCTTGCTGTCCTGCTCT7150.11937597253851753No Hit
GTGGCTGCACCATCTGTCTTCATCTTCCCGCCATCTGATGAGCAGTTGAAATCTGGAACTGCCTCTGTTGTGTGC7140.11920901313636575No Hit
GTCCAAGACCCCGCTAACCGCCACCCTCTCAAAATCCGGAAACACATTCC7110.11870813492991043No Hit
GTCTTCATCTTCCCGCCATCTGATGAGCAGTTGAAATCTGGAACTGCCTCTGTTGTGTGCCTGCTGAATAACTT7040.11753941911484803No Hit
GAGCAGGACAGCAAGGACAGCACCTACAGCCTCAGCAGCACCCTGACGCT7040.11753941911484803No Hit
GTGCAGCCACAGTTCGTTTGATCTCCACCTTGGCCCCTCCGCCGAAAGAG6940.11586982509333031No Hit
CAGCATGGACATGAGGGTCCCCGCTCAGCTCCTGGGGCTCCTGCTGCTCTGGTTCCCAGGTTCCAGATGCGACAT6930.11570286569117855No Hit
GCCCTGGACCAGGCAGGCGATGACCACGTTCCCATCTGGCTGGGTGCTGC6860.11453414987611613No Hit
CCTGTACACCACGAGCAGCCAGCTGACCCTGCCGGCCACACAGTGCCTAG6840.11420023107181257No Hit
GCACTGGAAGTCGCCGGTGTTTCCATTCGGTGATCAGCACTGAACACAGA6810.11369935286535725No Hit
GCATGGACATGAGGGTCCCCGCTCAGCTCCTGGGGCTCCTGCTGCTCTGG6790.11336543406105372No Hit
GAGTGGCTCCTGGGGGAAGAAGCCCTGGACCAGGCAGGCGATGACCACGT6720.1121967182459913No Hit
GTGTGCACGTGAGGTTCGCTTCTGAACCTAAGAGCAGGTCCTCGAGGGCC6650.11102800243092889No Hit
GGATGGGAGGGGGTCAGGCTGGAACTGAGGAGCAGGTGGGGGCACTTCTCCCTCTAACACTCTCCCCTGTTGAAG6500.1085236113986523No Hit
CCACTGTACTTTGGCCTCTCTGGGATAGAAGTTATTCAGCAGGCACACAACAGAGGCAGTTCCAGATTTCAACTG6400.10685401737713458No Hit
GAGTTTATTCAGGGGTGGGGACAGGCGGGCGGCTCAGTAGCAGGTGCCGT6310.10535138275776862No Hit
GTGGAGTCCCATCAAGATTCAGCGGCAGTGGATCTGGGACAGATTTCACT6270.10468354514916152No Hit
CTGCAGCACCCAGCCAGATGGGAACGTGGTCATCGCCTGCCTGGTCCAGG6260.10451658574700975No Hit
GAGTAACTACTGAATGTGAATCCAGACGCTGCACAGGAGAGTCTCAGGGACCTCCCAGGCTGGACCACGCCTCCC6230.10401570754055443No Hit
GATTTCACTCTCACCATCAGCAGCCTGCAGCCTGAAGATTTTGCAACTTACTTTTGTCAACAGGCTAACAGTTTC6220.10384874813840267No Hit
GGGTCCGACAGGCTCCAGGCAAGGGGCTACAGTGGGTGGCAGTTGTATCATTTGATGGAAGAAATAAAGACTACG6180.10318091052979557No Hit
GATGAAGACAGATGGTGCAGCCACAGTTCGTTTGATCTCCACCTTGGCCCCTCCGCCGAAAGAGAGCGGGAAA6170.1030139511276438No Hit
GTATGCTGGTCACAGCGAAGGTGGTGGTGCCCTGGCTGGGCTCCTGCCGG6130.10234611351903672No Hit
GCTCAGTAGCAGGTGCCGTCCACCTCCGCCATGACAACAGACACATTGAC6070.10134435710612608No Hit

[OK]Adapter Content

Adapter graph

Produced by FastQC (version 0.11.9)