FastQCFastQC Report
Sat 23 Apr 2022
EGAF00004684422

Summary

[OK]Basic Statistics

MeasureValue
FilenameEGAF00004684422
File typeConventional base calls
EncodingSanger / Illumina 1.9
Total Sequences1104769
Sequences flagged as poor quality0
Sequence length20-76
%GC57

[OK]Per base sequence quality

Per base quality graph

[OK]Per tile sequence quality

Per base quality graph

[OK]Per sequence quality scores

Per Sequence quality graph

[FAIL]Per base sequence content

Per base sequence content

[FAIL]Per sequence GC content

Per sequence GC content graph

[OK]Per base N content

N content graph

[WARN]Sequence Length Distribution

Sequence length distribution

[FAIL]Sequence Duplication Levels

Duplication level graph

[WARN]Overrepresented sequences

SequenceCountPercentagePossible Source
GCCCAAGGCTGCCCCCTCGGTCACTCTGTTCCCGCCCTCCTCTGAGGAGC34980.31662727683343755No Hit
GCCTTGGGCTGACCTAGGACGGTCAACTTGGTCCCTCCGCCGAACACTTT33260.3010584113058929No Hit
GTGTGGTGGTCTCCACTCCCGCCTTGACGGGGCTGCTATCTGCCTTCCAG28900.26159314752676804No Hit
CTCGTAATACTAATCGGCCCTCAGGGGTCCCTGACCGATTCTCTGCCTCCAAGTCTGGCACGTCAGCCTCCCTGG26850.24303723221777584No Hit
GACCTAGGACGGTCAACTTGGTCCCTCCGCCGAACACTTTCGAACCACTC26250.23760623261514396No Hit
CCTCAGAGGAGGGCGGGAACAGAGTGACCGAGGGGGCAGCCTTGGGCTGACCTAGGACGGTCAACTTGGTCCCTC25930.23470969949374032No Hit
CCTCTGAGGAGCTTCAAGCCAACAAGGCCACACTGGTGTGTCTCATAAGT25530.23108903309198575No Hit
GGTTATGATGTGCACTGGTACCAGCGGCTTCCAGGAACAGGCCCCAAACTCCTCATCTCTCGTAATACTAATCGG24080.2179641173856254No Hit
CCTATGAACATTCTGTAGGGGCCACTGTCTTCTCCACGGTGCTCCCTTCA23170.20972710132163377No Hit
GTAATAAACGGCCATGTCGTCAGATCTCAGGCTCCTCAGTTCCATGTAGGCTGTGTTCGTGGATGGGTCTGTGGT21980.19895561877641388No Hit
GTGCAGGGAGAAGGGCTTGATGCCTTGGGGTGGGAGGAGAGACCCCTCCCCTGGGATCCTGCAGCTCTAGTCTCC21090.19089963603250998No Hit
TCCTAGGTCAGCCCAAGGCTGCCCCCTCGGTCACTCTGTTCCCGCCCTCCTCTGAGGAGCTTCAAGCCAACAAGG20830.1885462028713695No Hit
GTATTACGAGAGATGAGGAGTTTGGGGCCTGTTCCTGGAAGCCGCTGGTA20780.18809361957115017No Hit
GGGCAAAGGGTCACCATCACCTGCACTGGGACCAGCTCCAACATCGGGGCAGGTTATGATGTGCACTGGTACCAG20270.18347726990891308No Hit
GTCTTCTCCACGGTGCTCCCTTCATGCGTGACCTGGCAGCTGTAGCTTTT19590.1773221370259303No Hit
GTGCAGTGACAGTCGGGGGTGGCAGCATGAGGGAGATGGGGTAGGTGGAG19410.17569283714514075No Hit
GTGCTCCCTTCATGCGTGACCTGGCAGCTGTAGCTTTTGTGGGACTTCCACTGCTCAGGCGTCAGGCTCAGGTAG19290.17460663722461436No Hit
GGGTTTATTGAGTGCAGGGAGAAGGGCTTGATGCCTTGGGGTGGGAGGAGAGACCCCTCCCCTGGGATCCTGCAG18540.16781788772132455No Hit
GGACAATCTCCAGCATGGCCTGGTCTCCTCTCCTCCTCACTCTCCTCGCTCACTGCACAGGGTCCTGGGCCCAGT18320.16582652120035954No Hit
GTCACATCCTGGCTGGGATTCGTGTAGTGCTTCACGTGGCATGTCACGGA18010.16302050473899973No Hit
CACCAGTGTGGCCTTGTTGGCTTGAAGCTCCTCAGAGGAGGGCGGGAACAGAGTGACCGAGGGGGCAGCCTTGGG17650.15976190497742063No Hit
CACCTGCACTGGGACCAGCTCCAACATCGGGGCAGGTTATGATGTGCACTGGTACCAGCGGCTTCCAGGAACAGG17630.1595808716573329No Hit
GTTTGGAGGGTGTGGTGGTCTCCACTCCCGCCTTGACGGGGCTGCTATCTGCCTTCCAGGCCACTGTCACGGCTC17470.15813260509663107No Hit
GTCCTAGGTCAGCCCAAGGCTGCCCCCTCGGTCACTCTGTTCCCGCCCTC16970.15360677209443782No Hit
GCTCAGGCGTCAGGCTCAGGTAGCTGCTGGCCGCGTACTTGTTGTTGCTTTGTTTGGAGGGTGTGGTGGTCTCCA16630.15052920565294645No Hit
CTCTGTTCCCGCCCTCCTCTGAGGAGCTTCAAGCCAACAAGGCCACACTGGTGTGTCTCATAAGTGACTTCTACC16600.15025765567281488No Hit
GTGTACAGGTCCCCGGAGGCATCCTGGCTGGGTGGGAAGTTTCTGGCGGT16600.15025765567281488No Hit
GTCACTGCACCGACCGGCCCTCGAGGACCTGCTCTTAGGTTCAGAAGCGAACCTCACGTGCACACTGACCGGC16090.14564130601057776No Hit
CATCATAACCTGCCCCGATGTTGGAGCTGGTCCCAGTGCAGGTGATGGTGACCCTTTGCCCTGGGGCCCCAGACA16080.14555078935053392No Hit
CTTCATGCGTGACCTGGCAGCTGTAGCTTTTGTGGGACTTCCACTGCTCAGGCGTCAGGCTCAGGTAGCTGCTGG15960.14446458943000753No Hit
GCTCAGTAGCAGGTGCCGTCCACCTCCGCCATGACAACAGACACATTGAC15920.14410252278983207No Hit
GAGAAGGGCTTGATGCCTTGGGGTGGGAGGAGAGACCCCTCCCCTGGGAT15830.1432878728494373No Hit
GTCTGGAGCTGAGGTGAAGAAGCCTGGGGCCTCAGTGAAGGTCTCCTGCAAGGCTTCTGGTTACACCTTTACCGA15510.14039133972803364No Hit
GTATGCTGGTCACAGCGAAGGTGGTGGTGCCCTGGCTGGGCTCCTGCCGG15390.13930513980750728No Hit
CTTATGAGACACACCAGTGTGGCCTTGTTGGCTTGAAGCTCCTCAGAGGA15350.13894307316733182No Hit
GAGTTTATTCAGGGGTGGGGACAGGCGGGCGGCTCAGTAGCAGGTGCCGT15340.13885255650728795No Hit
CCCTTCATGCGTGACCTGGCAGCTGTAGCTTTTGTGGGACTTCCACTGCTCAGGCGTCAGGCTCAGGTAGCTG15320.13867152318720022No Hit
CTCCCAGGTTCAGCTGGTGCAGTCTGGAGCTGAGGTGAAGAAGCCTGGGGCCTCAGTGAAGGTCTCCTGCAAGG14940.13523189010553338No Hit
GTGATGGCCAGGGAGGCTGACGTGCCAGACTTGGAGGCAGAGAATCGGTC14750.13351207356469993No Hit
GGCCATCACTGGGCTCCAGGCTGAGGATGAGGCTGACTATTACTGCCAGT14710.1331500069245245No Hit
GGATGAGGCTGACTATTACTGCCAGTCCTATGACATCAGCCTGAGTGGTT14510.13133967372364722No Hit
GACTATTACTGCCAGTCCTATGACATCAGCCTGAGTGGTTCGAAAGTGTTCGGCGGAGGGACCAAGTTGACCGTC14480.13106812374351562No Hit
GTTATGATGTGCACTGGTACCAGCGGCTTCCAGGAACAGGCCCCAAACTCCTCATCTCTCGTAATACTAATCGGC14460.1308870904234279No Hit
GTTTATTACTGTGCGAGAGATGGATGGAACGGTAGAAACTACCTAACTGA14300.12943882386272607No Hit
GTGTGACCTGGAGCGAAAGCGGACAGGGCGTGACCGCCAGAAACTTCCCACCCAGCCAGGATGCCTCCGGGGAC14230.128805207242419No Hit
GGTTTATTGAGTGCAGGGAGAAGGGCTTGATGCCTTGGGGTGGGAGGAGAGACCCCTCCCCTGGGATCCTGCAG14220.12871469058237514No Hit
CTTTCGAACCACTCAGGCTGATGTCATAGGACTGGCAGTAATAGTCAGCC14140.12799055730202422No Hit
AGGTCACACTGAGTGGCTCCTGGGGGAAGAAGCCCTGGACCAGGCAGGCG13950.1262707407611908No Hit
GGCTCAGGTAGCTGCTGGCCGCGTACTTGTTGTTGCTTTGTTTGGAGGGTGTGGTGGTCTCCACTCCCGCCTTGA13880.12563712414088374No Hit
GTTCAAGGACCACCTGAGCGTGACCTCTGTGGCTGCTACAGCGTGTCCAG13590.12301214099961168No Hit
GTCCAGGGCTTCTTCCCCCAGGAGCCACTCAGTGTGACCTGGAGCGAAAGCGGACAGGGCGTGACCGCCAGAAA13530.12246904103934848No Hit
GTCCTATGACATCAGCCTGAGTGGTTCGAAAGTGTTCGGCGGAGGGACCA13520.12237852437930463No Hit
GTGTCTGGGGCCCCAGGGCAAAGGGTCACCATCACCTGCACTGGGACCAG13450.12174490775899759No Hit
ACCTAGGACGGTCAACTTGGTCCCTCCGCCGAACACTTTCGAACCACTCA13240.11984405789807642No Hit
CCTTGGGAGAATCCCCTAGATCACAGCTCCTCACCATGGACTGGACCTGGAGCATCCTTTTCTTGGTGGCAGCAG13240.11984405789807642No Hit
GTCTTGGACTCGGGGTAGGCAGCAGTGCAAGTGAAGGTCTTCCCATGGTT13000.11767165805702368No Hit
GCTCTAGTCTCCCGTGGTGGGGGGTGAGGGTTGAGAACCTATGAACATTC12960.11730959141684823No Hit
GTTCGAAAGTGTTCGGCGGAGGGACCAAGTTGACCGTCCTAGGTCAGCCC12810.11595184151619027No Hit
GCTATCTGCCTTCCAGGCCACTGTCACGGCTCCCGGGTAGAAGTCACTTA12760.11549925821597094No Hit
ACATGGAACTGAGGAGCCTGAGATCTGACGACATGGCCGTTTATTACTGT12690.1148656415956639No Hit
GCCCCAAGGTCTTCCCGCTGAGCCTCTGCAGCACCCAGCCAGATGGGAAC12590.11396047499522524No Hit
CTATTACTGCCAGTCCTATGACATCAGCCTGAGTGGTTCGAAAGTGTTCG12440.11260272509456729No Hit
CCACCACACCCTCCAAACAAAGCAACAACAAGTACGCGGCCAGCAGCTAC12360.11187859181421636No Hit
GAGTGACCGAGGGGGCAGCCTTGGGCTGACCTAGGACGGTCAACTTGGTCCCTCCGCCGAACACTTTCGAACCA12330.11160704183408476No Hit
ACACTGGTGTGTCTCATAAGTGACTTCTACCCGGGAGCCGTGACAGTGGC12140.10988722529325136No Hit
GGATGTGACTGTGCCCTGCCCAGTTCCCTCAACTCCACCTACCCCATCTCCCTCAACTCCACCTACCCCATCTCC12070.10925360867294429No Hit
ATTCAGGGGTGGGGACAGGCGGGCGGCTCAGTAGCAGGTGCCGTCCACCT12010.1087105087126811No Hit
GCCTTGGGGTGGGAGGAGAGACCCCTCCCCTGGGATCCTGCAGCTCTAGT11890.10762430879215473No Hit
CTTCAGCTGTGGGCACAAGAGGCAGCACTCAGGACAATCTCCAGCATGGCCTGGTCTCCTCTCCTCCTCACTCTC11870.107443275472067No Hit
CAGTATGCTGGTCACAGCGAAGGTGGTGGTGCCCTGGCTGGGCTCCTGCC11770.10653810887162837No Hit
GTTCCCGCCCTCCTCTGAGGAGCTTCAAGCCAACAAGGCCACACTGGTGT11750.10635707555154063No Hit
GTGCCTAGCCGGCAAGTCCGTGACATGCCACGTGAAGCACTACACGAATCCCAGCCAGGATGTGACTGTGCCCTG11750.10635707555154063No Hit
CCCCTGGACAAGGGCTTGAGTGGATGGGATGGGTCAACCCCAACAATGGT11550.10454674235066334No Hit
GAGTGGCTCCTGGGGGAAGAAGCCCTGGACCAGGCAGGCGATGACCACGT11430.10346054243013697No Hit
CCCCTGGGATCCTGCAGCTCTAGTCTCCCGTGGTGGGGGGTGAGGGTTGAGAACCTATGAACATTCTGTAGGGGC11390.10309847578996152No Hit
GACTTCCACTGCTCAGGCGTCAGGCTCAGGTAGCTGCTGGCCGCGTACTT11330.10255537582969834No Hit
CTGTAGGGGCCACTGTCTTCTCCACGGTGCTCCCTTCATGCGTGACCTGG11320.10246485916965448No Hit
CTTATACACATCTCCGAGCCCACGAGACATGCGCAGATCTCGTATGCCGT11290.1021933091895229No Hit
GAACTGAGGAGCCTGAGATCTGACGACATGGCCGTTTATTACTGTGCGAGAGATGGATGGAACGGTAGAAACTAC11270.10201227586943515No Hit
GTAATAGTCAGCCTCATCCTCAGCCTGGAGCCCAGTGATGGCCAGGGAGG11220.10155969256921582No Hit
GTATCAACGCAGAGTACGGGACCCAAAAACCACACCCCTCCTTGGGAGAA11190.10128814258908424No Hit
GTGTGCACGTGAGGTTCGCTTCTGAACCTAAGAGCAGGTCCTCGAGGGCC11090.1003829759886456No Hit
GCTAGGCACTGTGTGGCCGGCAGGGTCAGCTGGCTGCTCGTGGTGTACAG11080.10029245932860172No Hit

[OK]Adapter Content

Adapter graph

[FAIL]Kmer Content

Kmer graph

SequenceCountPValueObs/Exp MaxMax Obs/Exp Position
AGGGGGG2650.059.77152670
GGCGTAA200.00590062954.72185567
AATGGTA2700.043.99848670
GTGGTAT1950.043.8463171
CTCTTAT1100.043.5274351
GTATCAA21750.040.5684281
CGTATGC2500.037.33337441
TCGTATG2500.037.2924140
GACAGGT2100.035.8287051
ACATGCG2800.034.2140727
CTTATAC2900.034.2001271
TCTTATA1300.034.1861952
TATCAAC26150.033.99012
CACGAGA2850.033.59121
ACGAGAC2800.032.96824322
ATCAACG26950.032.6005673
ATCTCGT2750.032.5290837
TCAACGC27250.032.241664
CCCCTAT751.7868751E-631.9201181
TCTCCGA2900.031.82852711
Produced by FastQC (version 0.11.5)