FastQCFastQC Report
Sat 23 Apr 2022
EGAF00004684423

Summary

[OK]Basic Statistics

MeasureValue
FilenameEGAF00004684423
File typeConventional base calls
EncodingSanger / Illumina 1.9
Total Sequences1104769
Sequences flagged as poor quality0
Sequence length20-76
%GC57

[OK]Per base sequence quality

Per base quality graph

[OK]Per tile sequence quality

Per base quality graph

[OK]Per sequence quality scores

Per Sequence quality graph

[FAIL]Per base sequence content

Per base sequence content

[FAIL]Per sequence GC content

Per sequence GC content graph

[OK]Per base N content

N content graph

[WARN]Sequence Length Distribution

Sequence length distribution

[FAIL]Sequence Duplication Levels

Duplication level graph

[WARN]Overrepresented sequences

SequenceCountPercentagePossible Source
GCCCAAGGCTGCCCCCTCGGTCACTCTGTTCCCGCCCTCCTCTGAGGAGC34950.316355726853306No Hit
GCCTTGGGCTGACCTAGGACGGTCAACTTGGTCCCTCCGCCGAACACTTT28560.25851558108527667No Hit
CTCGTAATACTAATCGGCCCTCAGGGGTCCCTGACCGATTCTCTGCCTCCAAGTCTGGCACGTCAGCCTCCCTGG27540.2492828817608025No Hit
GTGTGGTGGTCTCCACTCCCGCCTTGACGGGGCTGCTATCTGCCTTCCAG27210.24629583197935495No Hit
GTCCTGGGCCCAGTCTGTGCTGACGCAGCCGCCCTCAGTGTCTGGGGCCCCAGGGCAAAGGGTCACCATCACCTG25490.23072696645181026No Hit
CCTCTGAGGAGCTTCAAGCCAACAAGGCCACACTGGTGTGTCTCATAAGT25240.22846404995071368No Hit
GGGGGGGGGGGGGGGGGGGGGGGGGGGGGGGGGGGGGGGGGGGGGGGGGG24630.22294253368803796No Hit
GGTTATGATGTGCACTGGTACCAGCGGCTTCCAGGAACAGGCCCCAAACTCCTCATCTCTCGTAATACTAATCGG24630.22294253368803796No Hit
GACCTAGGACGGTCAACTTGGTCCCTCCGCCGAACACTTTCGAACCACTC21420.1938866858139575No Hit
GTAATAAACGGCCATGTCGTCAGATCTCAGGCTCCTCAGTTCCATGTAGGCTGTGTTCGTGGATGGGTCTGTGGT21300.1928004858934311No Hit
CCTCAGAGGAGGGCGGGAACAGAGTGACCGAGGGGGCAGCCTTGGGCTGACCTAGGACGGTCAACTTGGTCCCTC20950.18963240279189586No Hit
CCTATGAACATTCTGTAGGGGCCACTGTCTTCTCCACGGTGCTCCCTTCA20100.1819384866881674No Hit
GTGCAGGGAGAAGGGCTTGATGCCTTGGGGTGGGAGGAGAGACCCCTCCCCTGGGATCCTGCAGCTCTAGTCTCC19840.17958505352702692No Hit
GGGTTTATTGAGTGCAGGGAGAAGGGCTTGATGCCTTGGGGTGGGAGGAGAGACCCCTCCCCTGGGATCCTGCAG19770.17895143690671989No Hit
GGGCAAAGGGTCACCATCACCTGCACTGGGACCAGCTCCAACATCGGGGCAGGTTATGATGTGCACTGGTACCAG19460.17614542044536008No Hit
GGACAATCTCCAGCATGGCCTGGTCTCCTCTCCTCCTCACTCTCCTCGCTCACTGCACAGGGTCCTGGGCCCAGT19270.17442560390452663No Hit
GTCTTCTCCACGGTGCTCCCTTCATGCGTGACCTGGCAGCTGTAGCTTTT18630.16863253766171932No Hit
TCCTAGGTCAGCCCAAGGCTGCCCCCTCGGTCACTCTGTTCCCGCCCTCCTCTGAGGAGCTTCAAGCCAACAAGG18590.16827047102154385No Hit
GTGCTCCCTTCATGCGTGACCTGGCAGCTGTAGCTTTTGTGGGACTTCCACTGCTCAGGCGTCAGGCTCAGGTAG17790.16102913821803472No Hit
CACCTGCACTGGGACCAGCTCCAACATCGGGGCAGGTTATGATGTGCACTGGTACCAGCGGCTTCCAGGAACAGG17090.15469297201496424No Hit
GTGCAGTGACAGTCGGGGGTGGCAGCATGAGGGAGATGGGGTAGGTGGAG16830.15233953885382376No Hit
GTGTGACCTGGAGCGAAAGCGGACAGGGCGTGACCGCCAGAAACTTCCCACCCAGCCAGGATGCCTCCGGGGAC16270.1472706058913673No Hit
GTTTGGAGGGTGTGGTGGTCTCCACTCCCGCCTTGACGGGGCTGCTATCTGCCTTCCAGGCCACTGTCACGGCTC16160.14627492263088485No Hit
GTGCTGACGCAGCCGCCCTCAGTGTCTGGGGCCCCAGGGCAAAGGGTCAC16050.14527923937040232No Hit
GTCACATCCTGGCTGGGATTCGTGTAGTGCTTCACGTGGCATGTCACGGA15950.14437407276996367No Hit
GCTCAGGCGTCAGGCTCAGGTAGCTGCTGGCCGCGTACTTGTTGTTGCTTTGTTTGGAGGGTGTGGTGGTCTCCA15830.1432878728494373No Hit
CTCTGTTCCCGCCCTCCTCTGAGGAGCTTCAAGCCAACAAGGCCACACTGGTGTGTCTCATAAGTGACTTCTACC15240.1379473899068493No Hit
GTCCTAGGTCAGCCCAAGGCTGCCCCCTCGGTCACTCTGTTCCCGCCCTC15200.13758532326667386No Hit
GTATTACGAGAGATGAGGAGTTTGGGGCCTGTTCCTGGAAGCCGCTGGTA15120.13686118998632293No Hit
GTGTACAGGTCCCCGGAGGCATCCTGGCTGGGTGGGAAGTTTCTGGCGGT15050.1362275733660159No Hit
CTTCATGCGTGACCTGGCAGCTGTAGCTTTTGTGGGACTTCCACTGCTCAGGCGTCAGGCTCAGGTAGCTGCTGG15050.1362275733660159No Hit
CTGCACAGGGTCCTGGGCCCAGTCTGTGCTGACGCAGCCGCCCTCAGTGTCTGGGGCCCCAGGGCAAAGGGTCAC14540.13161122370377878No Hit
GGTTTATTGAGTGCAGGGAGAAGGGCTTGATGCCTTGGGGTGGGAGGAGAGACCCCTCCCCTGGGATCCTGCAG14500.13124915706360335No Hit
CTCCCAGGTTCAGCTGGTGCAGTCTGGAGCTGAGGTGAAGAAGCCTGGGGCCTCAGTGAAGGTCTCCTGCAAGG14480.13106812374351562No Hit
GACTATTACTGCCAGTCCTATGACATCAGCCTGAGTGGTTCGAAAGTGTTCGGCGGAGGGACCAAGTTGACCGTC14400.1303439904631647No Hit
GTCACTGCACCGACCGGCCCTCGAGGACCTGCTCTTAGGTTCAGAAGCGAACCTCACGTGCACACTGACCGGC14370.1300724404830331No Hit
GTCTGGAGCTGAGGTGAAGAAGCCTGGGGCCTCAGTGAAGGTCTCCTGCAAGGCTTCTGGTTACACCTTTACCGA14260.12907675722255058No Hit
CACCAGTGTGGCCTTGTTGGCTTGAAGCTCCTCAGAGGAGGGCGGGAACAGAGTGACCGAGGGGGCAGCCTTGGG14130.12790004064198035No Hit
CCCTTCATGCGTGACCTGGCAGCTGTAGCTTTTGTGGGACTTCCACTGCTCAGGCGTCAGGCTCAGGTAGCTG14100.12762849066184878No Hit
GAGAAGGGCTTGATGCCTTGGGGTGGGAGGAGAGACCCCTCCCCTGGGAT13960.12636125742123466No Hit
GTTATGATGTGCACTGGTACCAGCGGCTTCCAGGAACAGGCCCCAAACTCCTCATCTCTCGTAATACTAATCGGC13900.12581815746097147No Hit
GTTCAAGGACCACCTGAGCGTGACCTCTGTGGCTGCTACAGCGTGTCCAG13810.1250035075205767No Hit
GCACAGGGTCCTGGGCCCAGTCTGTGCTGACGCAGCCGCCCTCAGTGTCT13540.12255955769939236No Hit
GGCCATCACTGGGCTCCAGGCTGAGGATGAGGCTGACTATTACTGCCAGT13410.12138284111882212No Hit
GTGTCTGGGGCCCCAGGGCAAAGGGTCACCATCACCTGCACTGGGACCAG13330.12065870783847121No Hit
GTCCAGGGCTTCTTCCCCCAGGAGCCACTCAGTGTGACCTGGAGCGAAAGCGGACAGGGCGTGACCGCCAGAAA13300.12038715785833963No Hit
GTCCTATGACATCAGCCTGAGTGGTTCGAAAGTGTTCGGCGGAGGGACCA13220.1196630245779887No Hit
AGGTCACACTGAGTGGCTCCTGGGGGAAGAAGCCCTGGACCAGGCAGGCG13130.11884837463759393No Hit
GGATGAGGCTGACTATTACTGCCAGTCCTATGACATCAGCCTGAGTGGTT13080.1183957913373746No Hit
GTTCGAAAGTGTTCGGCGGAGGGACCAAGTTGACCGTCCTAGGTCAGCCC13040.11803372469719915No Hit
CTTATGAGACACACCAGTGTGGCCTTGTTGGCTTGAAGCTCCTCAGAGGA12900.11676649145658505No Hit
GGCTCAGGTAGCTGCTGGCCGCGTACTTGTTGTTGCTTTGTTTGGAGGGTGTGGTGGTCTCCACTCCCGCCTTGA12470.11287427507469887No Hit
GCCCCAAGGTCTTCCCGCTGAGCCTCTGCAGCACCCAGCCAGATGGGAAC12430.11251220843452342No Hit
CTTCAGCTGTGGGCACAAGAGGCAGCACTCAGGACAATCTCCAGCATGGCCTGGTCTCCTCTCCTCCTCACTCTC12340.11169755849412864No Hit
GTTTATTACTGTGCGAGAGATGGATGGAACGGTAGAAACTACCTAACTGA12290.11124497519390932No Hit
GCCTTGGGGTGGGAGGAGAGACCCCTCCCCTGGGATCCTGCAGCTCTAGT12220.11061135857360226No Hit
GCTCTAGTCTCCCGTGGTGGGGGGTGAGGGTTGAGAACCTATGAACATTC12160.11006825861333908No Hit
GAGTTTATTCAGGGGTGGGGACAGGCGGGCGGCTCAGTAGCAGGTGCCGT12070.10925360867294429No Hit
CTATTACTGCCAGTCCTATGACATCAGCCTGAGTGGTTCGAAAGTGTTCG12020.10880102537272499No Hit
GTTCCCGCCCTCCTCTGAGGAGCTTCAAGCCAACAAGGCCACACTGGTGT12000.10861999205263725No Hit
CATCATAACCTGCCCCGATGTTGGAGCTGGTCCCAGTGCAGGTGATGGTGACCCTTTGCCCTGGGGCCCCAGACA11870.107443275472067No Hit
ACACTGGTGTGTCTCATAAGTGACTTCTACCCGGGAGCCGTGACAGTGGC11830.10708120883189155No Hit
GTGATGGCCAGGGAGGCTGACGTGCCAGACTTGGAGGCAGAGAATCGGTC11650.105451908951102No Hit
CCACCACACCCTCCAAACAAAGCAACAACAAGTACGCGGCCAGCAGCTAC11650.105451908951102No Hit
GTATCAACGCAGAGTACGGGACCCAAAAACCACACCCCTCCTTGGGAGAA11630.10527087563101427No Hit
ACATGGAACTGAGGAGCCTGAGATCTGACGACATGGCCGTTTATTACTGT11590.10490880899083882No Hit
GCTCAGTAGCAGGTGCCGTCCACCTCCGCCATGACAACAGACACATTGAC11500.10409415905044403No Hit
CCTTGGGAGAATCCCCTAGATCACAGCTCCTCACCATGGACTGGACCTGGAGCATCCTTTTCTTGGTGGCAGCAG11480.1039131257303563No Hit
GAACTGAGGAGCCTGAGATCTGACGACATGGCCGTTTATTACTGTGCGAGAGATGGATGGAACGGTAGAAACTAC11190.10128814258908424No Hit
CTTTCGAACCACTCAGGCTGATGTCATAGGACTGGCAGTAATAGTCAGCC11150.10092607594890879No Hit
GGATGTGACTGTGCCCTGCCCAGTTCCCTCAACTCCACCTACCCCATCTCCCTCAACTCCACCTACCCCATCTCC11100.10047349264868946No Hit
GCTATCTGCCTTCCAGGCCACTGTCACGGCTCCCGGGTAGAAGTCACTTA11100.10047349264868946No Hit
GGTGTGTCTCATAAGTGACTTCTACCCGGGAGCCGTGACAGTGGCCTGGA11060.100111426008514No Hit

[OK]Adapter Content

Adapter graph

[WARN]Kmer Content

Kmer graph

SequenceCountPValueObs/Exp MaxMax Obs/Exp Position
CGTAGCT200.007663150351.22250714
GTGGTAT3450.038.6304471
GTATCAA22850.035.743411
ATATACT400.002734291834.137524
ATATCGC553.438437E-431.07489426
ACAGGTA1650.031.0664461
ATCAACG27400.029.5277083
TCAACGC27200.029.4938134
CAACGCA27600.029.3190465
TATCAAC27900.029.24592
AACGCAG27800.029.2309386
ATCGCAT605.702335E-428.50340528
GACAGGA2700.027.8447441
ACGCAGA29400.027.7562737
CGCAGAG29650.027.4070858
GCAGAGT30100.027.2242159
AGAGTAC30500.027.09106811
CAGAGTA30300.027.04451410
GAGTTTA4600.026.744161
TATCGCA659.1178576E-426.3036827
Produced by FastQC (version 0.11.5)