FastQCFastQC Report
Sat 23 Apr 2022
EGAF00004684424

Summary

[OK]Basic Statistics

MeasureValue
FilenameEGAF00004684424
File typeConventional base calls
EncodingSanger / Illumina 1.9
Total Sequences912892
Sequences flagged as poor quality0
Sequence length20-76
%GC54

[OK]Per base sequence quality

Per base quality graph

[OK]Per tile sequence quality

Per base quality graph

[OK]Per sequence quality scores

Per Sequence quality graph

[FAIL]Per base sequence content

Per base sequence content

[FAIL]Per sequence GC content

Per sequence GC content graph

[OK]Per base N content

N content graph

[WARN]Sequence Length Distribution

Sequence length distribution

[FAIL]Sequence Duplication Levels

Duplication level graph

[WARN]Overrepresented sequences

SequenceCountPercentagePossible Source
CTCCTGGGAGTTACCCGATTGGAGGGCGTTATCCACCTTCCACTGTACTTTGGCCTCTCTGGGATAGAAGTTATT25790.28250877431284316No Hit
GAGTTACACTACCCCGTGGACGTTCGGCCAAGGGACCAAGGTGGAAATCAAACGAACTGTGGCTGCACCAT25550.27987976671939285No Hit
CTCTAACACTCTCCCCTGTTGAAGCTCTTTGTGACGGGCGAGCTCAGGCC25190.27593625532921745No Hit
ATTATATACACTGGGTGCGACAGGCCCCTGGACAAGGGCTTGAGTGGATG24830.2719927439390421No Hit
GGGTAGTGTAACTCTGTTGACAGTAGTAAGTTGCAAAATCTTCAGGTTGCAGACTGCTGATGGTGACAGTGAAAT20980.2298190804607774No Hit
GATCTATGCTGCATCCAATTTGCAAACGGGGGTCCCATCAAGGTTCAGTGGCAGTGGATCTGGGACAGATTTCA18500.2026526686617913No Hit
GTGCAGTGACAGTCGGGGGTGGCAGCATGAGGGAGATGGGGTAGGTGGAG18350.2010095389158849No Hit
ACACTACCCCGTGGACGTTCGGCCAAGGGACCAAGGTGGAAATCAAACGA17660.1934511420847154No Hit
GTGTAACTCTGTTGACAGTAGTAAGTTGCAAAATCTTCAGGTTGCAGACTGCTGATGGTGACAGTGAAATCTGTC16920.18534503533824376No Hit
GCCCTAACAGTGGTGGCACAAACTTTGCACAGAAGTTTCAGGGCAGGGTC16680.18271602774479345No Hit
GGCTGGAACTGAGGAGCAGGTGGGGGCACTTCTCCCTCTAACACTCTCCC15870.1738431271168988No Hit
GTCTCAGTCAGGACACAGCATGGACATGAGGGTCCCCGCTCAGCTCCTGG15820.1732954172015967No Hit
GTCTGGGGCTGAGGTGAAGAAGCCTGGGGCCTCAGTGAGGGTCTCCTGCAAGGCTTCTGGATACACCTTCACCGA15600.17088549357426727No Hit
GTCCTGATCTATGCTGCATCCAATTTGCAAACGGGGGTCCCATCAAGGTT14770.16179350898025177No Hit
GTCACATCCTGGCTGGGATTCGTGTAGTGCTTCACGTGGCATGTCACGGA14770.16179350898025177No Hit
CAATAATACACGGCCGTGTCGTCAGATCTCAGCCTGCTCAGCTCCATGTA14690.16091717311576834No Hit
GTCACTGCACCGACCGGCCCTCGAGGACCTGCTCTTAGGTTCAGAAGCGAACCTCACGTGCACACTGACCGGC14520.15905495940374106No Hit
CTCCCAGGAGAGTGTCACAGAGCAGGACAGCAAGGACAGCACCTACAGCC14240.1559877838780491No Hit
GTATTATTGTGCGAGACGATACCTGGACCCGCCATTTTTCTACTACATGGATGTCTGGGGCACAGGGACCACGGT14060.1540160281829614No Hit
GTGTACAGGTCCCCGGAGGCATCCTGGCTGGGTGGGAAGTTTCTGGCGGT13850.15171564653869243No Hit
GTATGCTGGTCACAGCGAAGGTGGTGGTGCCCTGGCTGGGCTCCTGCCGG13630.14930572291136301No Hit
GTCCTGCTCTGTGACACTCTCCTGGGAGTTACCCGATTGGAGGGCGTTATCCACCTTCCACTGTACTTTGGCCT13570.14864847101300044No Hit
CTATTATATACACTGGGTGCGACAGGCCCCTGGACAAGGGCTTGAGTGGA13510.1479912191146379No Hit
GTAGGAGACAGAGTCACCATCACTTGCCGGGCAAGTCAGACCATTAGCACCTATTTAAATTGGTATCAGCAAAAA13460.14744350919933574No Hit
AGGTCACACTGAGTGGCTCCTGGGGGAAGAAGCCCTGGACCAGGCAGGCG13430.14711488325015445No Hit
TCCTTGGGGCTGAAGCCACGTGCCAGGCACGTCAGCGTCACCAGCTCGTT13400.14678625730097317No Hit
CAACAGAGGCAGTTCCAGATTTCAACTGCTCATCAGATGGCGGGAAGATG13350.14623854738567102No Hit
GCTCAGTAGCAGGTGCCGTCCACCTCCGCCATGACAACAGACACATTGAC13180.14437633367364375No Hit
CAATAGGGGTAGGTCCCCTGTGGAAAAAGGGTCAGAGGCCAAAGGATGGGAGGGGGTCAGGCTGGAACTGAGGAG13140.14393816574140206No Hit
CTTATACACATCTCCGAGCCCACGAGACGGACTCCTATCTCGTATGCCGT12540.13736564675777638No Hit
GTGTGACCTGGAGCGAAAGCGGACAGGGCGTGACCGCCAGAAACTTCCCACCCAGCCAGGATGCCTCCGGGGAC12530.13725610477471595No Hit
GGCGTAGAGTTTGTGTTTCTCGTAGTCTGCTTTGCTCAGCGTCAGGGTGC12510.13703702080859512No Hit
GCGTCAGGGTGCTGCTGAGGCTGTAGGTGCTGTCCTTGCTGTCCTGCTCT12420.13605114296105125No Hit
GGATGGGAGGGGGTCAGGCTGGAACTGAGGAGCAGGTGGGGGCACTTCTCCCTCTAACACTCTCCCCTGTTGAAG12220.1338603032998427No Hit
GCCTACATGGAGCTGAGCAGGCTGAGATCTGACGACACGGCCGTGTATTATTGTGCGAGACGATACCTGGACCCG11890.13024541785884858No Hit
GTCTCCATCCTCCCTGTCTGCATCTGTAGGAGACAGAGTCACCATCACTTGCCGGGCAAGTCAGACCATTAGCAC11740.12860228811294216No Hit
GAGTTTATTCAGGGGTGGGGACAGGCGGGCGGCTCAGTAGCAGGTGCCGT11560.12663053241785446No Hit
GTTCAAGGACCACCTGAGCGTGACCTCTGTGGCTGCTACAGCGTGTCCAG11490.12586373853643146No Hit
GCATGGACATGAGGGTCCCCGCTCAGCTCCTGGGGCTCCTGCTACTCTGG11480.12575419655337106No Hit
CTCTGGGATAGAAGTTATTCAGCAGGCACACAACAGAGGCAGTTCCAGAT11430.1252064866380689No Hit
GTCTTGGACTCGGGGTAGGCAGCAGTGCAAGTGAAGGTCTTCCCATGGTT11400.12487786068888762No Hit
ATAATACACGGCCGTGTCGTCAGATCTCAGCCTGCTCAGCTCCATGTAGGCTGTGCTGACGGACGTGTCCCTGGT11300.12378244085828334No Hit
CGTGCACACTGACCGGCCTGAGAGATGCCTCAGGTGTCACCTTCACCTGGACGCCCTCAAGTGGGAAGAGCG10900.11940076153586625No Hit
GTGTGCACGTGAGGTTCGCTTCTGAACCTAAGAGCAGGTCCTCGAGGGCC10770.11797671575608068No Hit
CTGCTACTCTGGCTCCGAGGTGCCAGATGTGACATCCAGATGACCCAGTC10720.11742900584077853No Hit
GACCTGGAGGGTCCTCTTCTTGGTGGCAGCAGGCACAGGAGCCCACTCCCAGGTGCAGCTGGTGCAGTCTGGGG10710.11731946385771812No Hit
GATCAGGACCTTAGGGGCTTTCCCTGGTTTTTGCTGATACCAATTTAAAT10680.11699083790853683No Hit
GTCCAGGGCTTCTTCCCCCAGGAGCCACTCAGTGTGACCTGGAGCGAAAGCGGACAGGGCGTGACCGCCAGAAA10640.11655266997629513No Hit
GCATAGATCAGGACCTTAGGGGCTTTCCCTGGTTTTTGCTGATACCAATT10530.1153477081626304No Hit
GTGCCTAGCCGGCAAGTCCGTGACATGCCACGTGAAGCACTACACGAATCCCAGCCAGGATGTGACTGTGCCCTG10310.112937784535301No Hit
GTACAGTGGAAGGTGGATAACGCCCTCCAATCGGGTAACTCCCAGGAGAG10260.11239007461999886No Hit
GTCTTCATCTTCCCGCCATCTGATGAGCAGTTGAAATCTGGAACTGCCTCTGTTGTGTGCCTGCTGAATAACTT10170.11140419677245501No Hit
CCACTGTACTTTGGCCTCTCTGGGATAGAAGTTATTCAGCAGGCACACAACAGAGGCAGTTCCAGATTTCAACTG10160.11129465478939458No Hit
GCTAGGCACTGTGTGGCCGGCAGGGTCAGCTGGCTGCTCGTGGTGTACAG10030.10987060900960902No Hit
GCCCCAAGGTCTTCCCGCTGAGCCTCTGCAGCACCCAGCCAGATGGGAAC9990.10943244107736731No Hit
CCCCTGGACAAGGGCTTGAGTGGATGGGATGGATCAGCCCTAACAGTGGT9810.10746068538227962No Hit
GTGGCTGCACCATCTGTCTTCATCTTCCCGCCATCTGATGAGCAGTTGAAATCTGGAACTGCCTCTGTTGTGTGC9790.10724160141615875No Hit
GGATGTGACTGTGCCCTGCCCAGTTCCCTCAACTCCACCTACCCCATCTCCCTCAACTCCACCTACCCCATCTCC9690.10614618158555449No Hit
CTGTTAGGGCTGATCCATCCCATCCACTCAAGCCCTTGTCCAGGGGCCTG9670.10592709761943363No Hit
CCTTGGGGCTGGTCGGGGATGCTGAGGAGACGGAGACCGTGGTCCCTGTG9530.10439350985658763No Hit
CAGTATGCTGGTCACAGCGAAGGTGGTGGTGCCCTGGCTGGGCTCCTGCC9430.10329809002598336No Hit
GGCTGTAGGTGCTGTCCTTGCTGTCCTGCTCTGTGACACTCTCCTGGGAGTTACCCGATTGGAGGGCGTTATCCA9300.10187404424619781No Hit
GTGCAGCCACAGTTCGTTTGATTTCCACCTTGGTCCCTTGGCCGAACGTC9180.10055954044947267No Hit

[OK]Adapter Content

Adapter graph

[FAIL]Kmer Content

Kmer graph

SequenceCountPValueObs/Exp MaxMax Obs/Exp Position
TCGTATG2200.043.98344840
TATCTCG2100.042.5857836
CGTATGC2200.042.45736741
ATGCCGT2350.041.38493344
CGGACTC2400.041.3043528
GTGGTAT1700.040.160091
TTTCGAT808.647866E-539.2955170
CTTATAC2450.039.0126571
GCCGTCT2450.038.38292746
ACTCCTA2500.038.3564331
ACGAGAC2600.038.06859622
TCTCCGA2600.038.05411
GTATCAA21400.037.6453931
CCCACGA2550.037.46632819
CACGAGA2650.037.3462321
ATCTCGT2450.036.53841837
CCGTCTT2600.036.20699347
TTATACA2850.035.913032
GTATGCC2800.035.86827542
ATCTCCG2800.035.33585710
Produced by FastQC (version 0.11.5)