FastQCFastQC Report
Sat 23 Apr 2022
EGAF00004684425

Summary

[OK]Basic Statistics

MeasureValue
FilenameEGAF00004684425
File typeConventional base calls
EncodingSanger / Illumina 1.9
Total Sequences912892
Sequences flagged as poor quality0
Sequence length20-76
%GC54

[OK]Per base sequence quality

Per base quality graph

[OK]Per tile sequence quality

Per base quality graph

[OK]Per sequence quality scores

Per Sequence quality graph

[FAIL]Per base sequence content

Per base sequence content

[FAIL]Per sequence GC content

Per sequence GC content graph

[OK]Per base N content

N content graph

[WARN]Sequence Length Distribution

Sequence length distribution

[FAIL]Sequence Duplication Levels

Duplication level graph

[WARN]Overrepresented sequences

SequenceCountPercentagePossible Source
CTCCTGGGAGTTACCCGATTGGAGGGCGTTATCCACCTTCCACTGTACTTTGGCCTCTCTGGGATAGAAGTTATT25560.2799893087024533No Hit
GGGGGGGGGGGGGGGGGGGGGGGGGGGGGGGGGGGGGGGGGGGGGGGGGG25230.27637442326145917No Hit
GAGTTACACTACCCCGTGGACGTTCGGCCAAGGGACCAAGGTGGAAATCAAACGAACTGTGGCTGCACCAT23790.2606003777007576No Hit
ATTATATACACTGGGTGCGACAGGCCCCTGGACAAGGGCTTGAGTGGATG22510.24657900386902284No Hit
CTCTAACACTCTCCCCTGTTGAAGCTCTTTGTGACGGGCGAGCTCAGGCC21650.23715839332582606No Hit
GGGTAGTGTAACTCTGTTGACAGTAGTAAGTTGCAAAATCTTCAGGTTGCAGACTGCTGATGGTGACAGTGAAAT19730.21612633257822395No Hit
ACACTACCCCGTGGACGTTCGGCCAAGGGACCAAGGTGGAAATCAAACGA18050.19772327942407208No Hit
GATCTATGCTGCATCCAATTTGCAAACGGGGGTCCCATCAAGGTTCAGTGGCAGTGGATCTGGGACAGATTTCA17510.19180801233880895No Hit
GTGTAACTCTGTTGACAGTAGTAAGTTGCAAAATCTTCAGGTTGCAGACTGCTGATGGTGACAGTGAAATCTGTC17460.19126030242350683No Hit
GTCCTGATCTATGCTGCATCCAATTTGCAAACGGGGGTCCCATCAAGGTT15840.17351450116771752No Hit
GTCTCAGTCAGGACACAGCATGGACATGAGGGTCCCCGCTCAGCTCCTGG15710.172090455387932No Hit
GCCCTAACAGTGGTGGCACAAACTTTGCACAGAAGTTTCAGGGCAGGGTC15460.1693519058114213No Hit
CTCCCAGGAGAGTGTCACAGAGCAGGACAGCAAGGACAGCACCTACAGCC15110.16551793640430632No Hit
CAATAATACACGGCCGTGTCGTCAGATCTCAGCCTGCTCAGCTCCATGTA14740.16146488303107048No Hit
GTGCAGTGACAGTCGGGGGTGGCAGCATGAGGGAGATGGGGTAGGTGGAG14560.15949312733598278No Hit
GTCTGGGGCTGAGGTGAAGAAGCCTGGGGCCTCAGTGAGGGTCTCCTGCAAGGCTTCTGGATACACCTTCACCGA14420.1579595395731368No Hit
GTGTACAGGTCCCCGGAGGCATCCTGGCTGGGTGGGAAGTTTCTGGCGGT14090.15434465413214268No Hit
GTTCAAGGACCACCTGAGCGTGACCTCTGTGGCTGCTACAGCGTGTCCAG13770.150839310674209No Hit
GTATTATTGTGCGAGACGATACCTGGACCCGCCATTTTTCTACTACATGGATGTCTGGGGCACAGGGACCACGGT13610.14908663894524216No Hit
GTGTGACCTGGAGCGAAAGCGGACAGGGCGTGACCGCCAGAAACTTCCCACCCAGCCAGGATGCCTCCGGGGAC13360.14634808936873145No Hit
GTCACATCCTGGCTGGGATTCGTGTAGTGCTTCACGTGGCATGTCACGGA13220.14481450160588547No Hit
GTCCTGCTCTGTGACACTCTCCTGGGAGTTACCCGATTGGAGGGCGTTATCCACCTTCCACTGTACTTTGGCCT13090.1433904558260999No Hit
GTAGGAGACAGAGTCACCATCACTTGCCGGGCAAGTCAGACCATTAGCACCTATTTAAATTGGTATCAGCAAAAA12830.1405423642665288No Hit
AGGTCACACTGAGTGGCTCCTGGGGGAAGAAGCCCTGGACCAGGCAGGCG12820.14043282228346837No Hit
GGCTGGAACTGAGGAGCAGGTGGGGGCACTTCTCCCTCTAACACTCTCCC12750.13966602840204537No Hit
ATAATACACGGCCGTGTCGTCAGATCTCAGCCTGCTCAGCTCCATGTAGGCTGTGCTGACGGACGTGTCCCTGGT12470.1365988528763534No Hit
CAACAGAGGCAGTTCCAGATTTCAACTGCTCATCAGATGGCGGGAAGATG12460.13648931089329297No Hit
GCCTACATGGAGCTGAGCAGGCTGAGATCTGACGACACGGCCGTGTATTATTGTGCGAGACGATACCTGGACCCG12440.1362702269271721No Hit
GTCACTGCACCGACCGGCCCTCGAGGACCTGCTCTTAGGTTCAGAAGCGAACCTCACGTGCACACTGACCGGC12160.13320305140148014No Hit
GGCGTAGAGTTTGTGTTTCTCGTAGTCTGCTTTGCTCAGCGTCAGGGTGC12010.1315599216555737No Hit
CTATTATATACACTGGGTGCGACAGGCCCCTGGACAAGGGCTTGAGTGGA11720.1283832041468213No Hit
CTGTTAGGGCTGATCCATCCCATCCACTCAAGCCCTTGTCCAGGGGCCTG11660.12772595224845876No Hit
GTCTCCATCCTCCCTGTCTGCATCTGTAGGAGACAGAGTCACCATCACTTGCCGGGCAAGTCAGACCATTAGCAC11570.1267400744009149No Hit
GTCCAGGGCTTCTTCCCCCAGGAGCCACTCAGTGTGACCTGGAGCGAAAGCGGACAGGGCGTGACCGCCAGAAA11200.12268702102767907No Hit
GCGTCAGGGTGCTGCTGAGGCTGTAGGTGCTGTCCTTGCTGTCCTGCTCT11110.12170114318013521No Hit
GGATGGGAGGGGGTCAGGCTGGAACTGAGGAGCAGGTGGGGGCACTTCTCCCTCTAACACTCTCCCCTGTTGAAG10720.11742900584077853No Hit
CTCTGGGATAGAAGTTATTCAGCAGGCACACAACAGAGGCAGTTCCAGAT10590.11600496006099299No Hit
GTGGCTGCACCATCTGTCTTCATCTTCCCGCCATCTGATGAGCAGTTGAAATCTGGAACTGCCTCTGTTGTGTGC10530.1153477081626304No Hit
CAATAGGGGTAGGTCCCCTGTGGAAAAAGGGTCAGAGGCCAAAGGATGGGAGGGGGTCAGGCTGGAACTGAGGAG10520.11523816617956999No Hit
GTCTTCATCTTCCCGCCATCTGATGAGCAGTTGAAATCTGGAACTGCCTCTGTTGTGTGCCTGCTGAATAACTT10460.11458091428120742No Hit
CGTGCACACTGACCGGCCTGAGAGATGCCTCAGGTGTCACCTTCACCTGGACGCCCTCAAGTGGGAAGAGCG10270.1124996166030593No Hit
CCTTGGGGCTGGTCGGGGATGCTGAGGAGACGGAGACCGTGGTCCCTGTG10200.1117328227216363No Hit
GTACAGTGGAAGGTGGATAACGCCCTCCAATCGGGTAACTCCCAGGAGAG10010.10965152504348817No Hit
GCTCAGTAGCAGGTGCCGTCCACCTCCGCCATGACAACAGACACATTGAC9990.10943244107736731No Hit
GAGCAGGACAGCAAGGACAGCACCTACAGCCTCAGCAGCACCCTGACGCT9980.10932289909430688No Hit
CTGCTACTCTGGCTCCGAGGTGCCAGATGTGACATCCAGATGACCCAGTC9950.10899427314512561No Hit
GCCCCAAGGTCTTCCCGCTGAGCCTCTGCAGCACCCAGCCAGATGGGAAC9950.10899427314512561No Hit
GTCCAAGACCCCGCTAACCGCCACCCTCTCAAAATCCGGAAACACATTCC9910.10855610521288389No Hit
GCATAGATCAGGACCTTAGGGGCTTTCCCTGGTTTTTGCTGATACCAATT9860.10800839529758176No Hit
GCATGGACATGAGGGTCCCCGCTCAGCTCCTGGGGCTCCTGCTACTCTGG9850.10789885331452131No Hit
TCCTTGGGGCTGAAGCCACGTGCCAGGCACGTCAGCGTCACCAGCTCGTT9840.10778931133146089No Hit
GTCTTGGACTCGGGGTAGGCAGCAGTGCAAGTGAAGGTCTTCCCATGGTT9780.10713205943309832No Hit
GATCAGGACCTTAGGGGCTTTCCCTGGTTTTTGCTGATACCAATTTAAAT9680.10603663960249406No Hit
CCACTGTACTTTGGCCTCTCTGGGATAGAAGTTATTCAGCAGGCACACAACAGAGGCAGTTCCAGATTTCAACTG9530.10439350985658763No Hit
GGATGTGACTGTGCCCTGCCCAGTTCCCTCAACTCCACCTACCCCATCTCCCTCAACTCCACCTACCCCATCTCC9520.1042839678735272No Hit
GAGTTTATTCAGGGGTGGGGACAGGCGGGCGGCTCAGTAGCAGGTGCCGT9430.10329809002598336No Hit
GTGCAGCCACAGTTCGTTTGATTTCCACCTTGGTCCCTTGGCCGAACGTC9380.10275038011068122No Hit
GCCCTGGACCAGGCAGGCGATGACCACGTTCCCATCTGGCTGGGTGCTGC9290.10176450226313738No Hit
GAGTGGCTCCTGGGGGAAGAAGCCCTGGACCAGGCAGGCGATGACCACGT9290.10176450226313738No Hit
CCTGTACACCACGAGCAGCCAGCTGACCCTGCCGGCCACACAGTGCCTAG9280.10165496028007694No Hit
GGGTGCGACAGGCCCCTGGACAAGGGCTTGAGTGGATGGGATGGATCAGCCCTAACAGTGGTGGCACAAACTTTG9250.10132633433089565No Hit
GGCTGTAGGTGCTGTCCTTGCTGTCCTGCTCTGTGACACTCTCCTGGGAGTTACCCGATTGGAGGGCGTTATCCA9240.10121679234783523No Hit

[OK]Adapter Content

Adapter graph

[WARN]Kmer Content

Kmer graph

SequenceCountPValueObs/Exp MaxMax Obs/Exp Position
CTAGCTC350.001439280938.904533
GTATCAA21350.035.116321
GTGGTAT2650.030.8638151
TATCAAC24200.030.670492
CCGTCAG8350.030.64278670
GAGTTTA3750.029.989341
ATCGTTG703.7901948E-529.63945840
AATCGTT703.82632E-529.59828839
ATCAACG25250.029.3902533
AACGCAG25750.028.9612876
TCAACGC26150.028.3787364
AGTTTAT4000.028.0888212
CAACGCA26350.028.0387545
GCGTAGA13700.027.7752270
ACGCAGA27400.027.2172667
CGCAGAG27750.026.8739878
AGAGTAC28500.026.04729511
GCAGAGT29150.025.7001179
TCTCGCG6050.024.941570
GGGTATA700.001432572324.3419971
Produced by FastQC (version 0.11.5)