FastQCFastQC Report
Sat 23 Apr 2022
EGAF00004684426

Summary

[OK]Basic Statistics

MeasureValue
FilenameEGAF00004684426
File typeConventional base calls
EncodingSanger / Illumina 1.9
Total Sequences1142538
Sequences flagged as poor quality0
Sequence length20-76
%GC55

[OK]Per base sequence quality

Per base quality graph

[OK]Per tile sequence quality

Per base quality graph

[OK]Per sequence quality scores

Per Sequence quality graph

[FAIL]Per base sequence content

Per base sequence content

[FAIL]Per sequence GC content

Per sequence GC content graph

[OK]Per base N content

N content graph

[WARN]Sequence Length Distribution

Sequence length distribution

[FAIL]Sequence Duplication Levels

Duplication level graph

[WARN]Overrepresented sequences

SequenceCountPercentagePossible Source
CTCCTGGGAGTTACCCGATTGGAGGGCGTTATCCACCTTCCACTGTACTTTGGCCTCTCTGGGATAGAAGTTATT50400.44112318364903397No Hit
CTCTAACACTCTCCCCTGTTGAAGCTCTTTGTGACGGGCGAGCTCAGGCC48930.4282570907926038No Hit
ATGTTATAGTAGTCCGCTCACTTTCGGCGGAGGGACCAGGGTGGAGATCAAACGAACTGTGGCTGCACCAT43460.3803812214560916No Hit
GTCTCTGGGCGAGAGGGCCACCATCAACTGCAAGTCCAGCCGGAGTGTTTTCTACAGTTCCGACAATAAGAACTA38460.33661900085598906No Hit
GGCGTAGACTTTGTGTTTCTCGTAGTCTGCTTTGCTCAGCGTCAGGGTGC35760.3129874017319336No Hit
GGCTGGAACTGAGGAGCAGGTGGGGGCACTTCTCCCTCTAACACTCTCCC33080.2895308514902787No Hit
CAACAGAGGCAGTTCCAGATTTCAACTGCTCATCAGATGGCGGGAAGATG30970.27106319439703536No Hit
CTCCCAGGAGAGTGTCACAGAGCAGGACAGCAAGGACAGCACCTACAGCC30290.2651115323954214No Hit
GTCTACGCCTGCGAAGTCACCCATCAGGGCCTGAGCTCGCCCGTCACAAA29120.25487117277499743No Hit
GTCCTGCTCTGTGACACTCTCCTGGGAGTTACCCGATTGGAGGGCGTTATCCACCTTCCACTGTACTTTGGCCT27530.2409547866241648No Hit
GTACAGTGGAAGGTGGATAACGCCCTCCAATCGGGTAACTCCCAGGAGAG27110.23727876009375617No Hit
CAATAGGGGTAGGTCCCCTGTGGAAAAAGGGTCAGAGGCCAAAGGATGGGAGGGGGTCAGGCTGGAACTGAGGAG26990.23622846679935372No Hit
GCGTCAGGGTGCTGCTGAGGCTGTAGGTGCTGTCCTTGCTGTCCTGCTCT26580.2326399647101453No Hit
GTGTTGCAGACCCAGGTCTTCATTTCTCTGTTGCTCTGGATCTCTGGTGC26440.2314146225333424No Hit
GGATGGGAGGGGGTCAGGCTGGAACTGAGGAGCAGGTGGGGGCACTTCTCCCTCTAACACTCTCCCCTGTTGAAG26400.2310645247685416No Hit
GTAATACACAGCCGTGTCCTCGGCCCCCAGACTGTTCATCTGCAGATAAA26270.22992670703293897No Hit
GTTTTCTACAGTTCCGACAATAAGAACTACCTTGCTTGGTACCAGCAGAAGCCAGGGCAGCCTCCTAAATTGCTC26060.22808869376773466No Hit
GTTTATTACTGTCAGCAATGTTATAGTAGTCCGCTCACTTTCGGCGGAGGGACCAGGGTGGAGATCAAACGAA24930.21819843191211147No Hit
GTCTTCATCTTCCCGCCATCTGATGAGCAGTTGAAATCTGGAACTGCCTCTGTTGTGTGCCTGCTGAATAACTT24300.21268439211649853No Hit
ATTTAGGAGGCTGCCCTGGCTTCTGCTGGTACCAAGCAAGGTAGTTCTTA23570.20629510790888356No Hit
GTCTCCAGACTCCCTGGCTCTGTCTCTGGGCGAGAGGGCCACCATCAACTGCAAGTCCAGCCGGAGTGTTTTCTA23370.20454461908487945No Hit
CTATAACATTGCTGACAGTAATAAACTGCCACATCTTCAGCCTGCAGGCTGCTGATGGTGAGAGTGAAATCTGTC22950.20086859255447082No Hit
CCACTGTACTTTGGCCTCTCTGGGATAGAAGTTATTCAGCAGGCACACAACAGAGGCAGTTCCAGATTTCAACTG22950.20086859255447082No Hit
CTCCTAAATTGCTCCTTTCCTGGGCATCTACCCGGGAATCCGGGGTCCCT22700.19868048152446569No Hit
GTTATAGTAGTCCGCTCACTTTCGGCGGAGGGACCAGGGTGGAGATCAAA22490.19684246825926138No Hit
CCCTGGCTCTGTCTCTGGGCGAGAGGGCCACCATCAACTGCAAGTCCAGC22030.19281634396405195No Hit
GTACCAGCAGAAGCCAGGGCAGCCTCCTAAATTGCTCCTTTCCTGGGCATCTACCCGGGAATCCGGGGTCCCTGA21870.19141595290484867No Hit
CTCTGGGATAGAAGTTATTCAGCAGGCACACAACAGAGGCAGTTCCAGAT21500.18817754858044108No Hit
GTCCCTGACCGATTCAGTGGCAGCGGGTCTGGGACAGATTTCACTCTCAC21250.18598943755043595No Hit
ACTATAACATTGCTGACAGTAATAAACTGCCACATCTTCAGCCTGCAGGCTGCTGATGGTGAGAGTGAAAT20370.17828728672481792No Hit
GTGTATTACTGTTCAAGAGGGGATGGGCCGCTCTGGCACCTCAATCTCTGGGGCCGTGGCACCCTGGTCA19900.17417363798840826No Hit
GTGGCTGCACCATCTGTCTTCATCTTCCCGCCATCTGATGAGCAGTTGAAATCTGGAACTGCCTCTGTTGTGTGC19700.17242314916440415No Hit
GGCCAAAGGATGGGAGGGGGTCAGGCTGGAACTGAGGAGCAGGTGGGGGCACTTCTCCCTCTAACACTCTCCC19680.17224810028200374No Hit
GGCTGTAGGTGCTGTCCTTGCTGTCCTGCTCTGTGACACTCTCCTGGGAGTTACCCGATTGGAGGGCGTTATCCA19660.17207305139960333No Hit
GATTTCACTCTCACCATCAGCAGCCTGCAGGCTGAAGATGTGGCAGTTTATTACTGTCAGCAATGTTATAGTAGT19390.1697098914871978No Hit
CTTATACACATCTCCGAGCCCACGAGACTAGCGCTCATCTCGTATGCCGT19370.1695348426047974No Hit
ATAGTAGTCCGCTCACTTTCGGCGGAGGGACCAGGGTGGAGATCAAACGA18790.16445842501518548No Hit
GTAATAAACTGCCACATCTTCAGCCTGCAGGCTGCTGATGGTGAGAGTGAAATCTGTCCCAGACCCGCTGCCA17830.1560560786599658No Hit
GAGCAGGACAGCAAGGACAGCACCTACAGCCTCAGCAGCACCCTGACGCT17710.15500578536556334No Hit
ATCCCAGAGAGGCCAAAGTACAGTGGAAGGTGGATAACGCCCTCCAATCG17530.15343034542395964No Hit
GAACTACCTTGCTTGGTACCAGCAGAAGCCAGGGCAGCCTCCTAAATTGC17530.15343034542395964No Hit
GTAGAAAACACTCCGGCTGGACTTGCAGTTGATGGTGGCCCTCTCGCCCAGAGACAGAGCCAGGGAGTCTGGAGA16800.14704106121634467No Hit
GGCCCTGATGGGTGACTTCGCAGGCGTAGACTTTGTGTTTCTCGTAGTCTGCTTTGCTCAGCGTCAGGGTGCTG16670.145903243480742No Hit
GCTTTGCTCAGCGTCAGGGTGCTGCTGAGGCTGTAGGTGCTGTCCTTGCT16590.14520304795114036No Hit
GATGAAGACAGATGGTGCAGCCACAGTTCGTTTGATCTCCACCCTGGTCC16520.14459037686273893No Hit
GTGCAGCCACAGTTCGTTTGATCTCCACCCTGGTCCCTCCGCCGAAAGTG16420.14371513245073686No Hit
GGCAGGGGCAGCAAGATGGTGTTGCAGACCCAGGTCTTCATTTCTCTGTT16370.14327751024473584No Hit
GTCAGGGACCCCGGATTCCCGGGTAGATGCCCAGGAAAGGAGCAATTTAGGAGGCTGCCCTGGCTTCTGCTGGTA15820.13846366597872456No Hit
GATCAGGACTGCACACAGAGAACTCACCATGGAGTTTGGGCTGATCTGCG15720.13758842156672252No Hit
GTCACATCCTGGCTGGGATTCGTGTAGTGCTTCACGTGGCATGTCACGGA15460.13531278609551717No Hit
GCAGAAGCCAGGGCAGCCTCCTAAATTGCTCCTTTCCTGGGCATCTACCCGGGAATCCGGGGTCCCTGACCGATT15420.13496268833071637No Hit
GTTGTGTGCCTGCTGAATAACTTCTATCCCAGAGAGGCCAAAGTACAGTG15390.13470011500711573No Hit
GGCTTCTGCTGGTACCAAGCAAGGTAGTTCTTATTGTCGGAACTGTAGAA15160.13268705285951102No Hit
GGGTGGAGATCAAACGAACTGTGGCTGCACCATCTGTCTTCATCTTCCCG15150.13259952841831082No Hit
GTACTTTGGCCTCTCTGGGATAGAAGTTATTCAGCAGGCACACAACAGAGGCAGTTCCAGATTTCAACTGCTCAT15140.1325120039771106No Hit
GTTGAAGCTCTTTGTGACGGGCGAGCTCAGGCCCTGATGGGTGACTTCGC15080.1319868573299094No Hit
ATTCAGTGGCAGCGGGTCTGGGACAGATTTCACTCTCACCATCAGCAGCC14970.13102408847670713No Hit
ACCTTGCTTGGTACCAGCAGAAGCCAGGGCAGCCTCCTAAATTGCTCCTT14960.1309365640355069No Hit
AGCCAGGGCAGCCTCCTAAATTGCTCCTTTCCTGGGCATCTACCCGGGAA14890.13032389294710547No Hit
GTCCTTGCTGTCCTGCTCTGTGACACTCTCCTGGGAGTTACCCGATTGGAGGGCGTTATCCACCTTCCACTGTA14790.1294486485351034No Hit
CTCCTGTGCAGCCTCTGGATTCACCTTCAGAAGTTACTGGATGCACTGGGTCCGCCAAGCTCCAGGGAAGGGG14620.12796073303469993No Hit
GACTACTATAACATTGCTGACAGTAATAAACTGCCACATCTTCAGCCTGCAGGCTGCTGATGGTGAGAGTGAAAT14560.12743558638749872No Hit
CTGCTGGTACCAAGCAAGGTAGTTCTTATTGTCGGAACTGTAGAAAACAC14340.1255100486810942No Hit
GGATCTCTGGTGCCTACGGGGACATCGTGATGACCCAGTCTCCAGACTCCCTGGCTCTGTCTCTGGGCGAGAGGG14320.12533499979869378No Hit
GTTGAAATCTGGAACTGCCTCTGTTGTGTGCCTGCTGAATAACTTCTATC14070.12314688876868865No Hit
ACCTTCCACTGTACTTTGGCCTCTCTGGGATAGAAGTTATTCAGCAGGCA13920.12183402215068559No Hit
CCCCTGTTGAAGCTCTTTGTGACGGGCGAGCTCAGGCCCTGATGGGTGACTTCGCAGGCGTAGACTTTGTGTTT13900.12165897326828516No Hit
GTGGAAAAAGGGTCAGAGGCCAAAGGATGGGAGGGGGTCAGGCTGGAACTGAGGAGCAGGTGGGGGCACTTCTCC13890.12157144882708497No Hit
GTGTACAGGTCCCCGGAGGCATCCTGGCTAGGTGGGAAGTTTCTGGCGGT13800.12078372885628312No Hit
GAGCAAAGCAGACTACGAGAAACACAAAGTCTACGCCTGCGAAGTCACCC13560.11868314226747818No Hit
CTTCTATCCCAGAGAGGCCAAAGTACAGTGGAAGGTGGATAACGCCCTCCAATCGGGTAACTCCCAGGAGAGTGT13480.11798294673787656No Hit
GTGCAGCGACAGTCGGGGGTGGCAGCATGGGGGAGGTGGGGGAACTGGGC13420.11745780009067533No Hit
CCCAGAGACAGAGCCAGGGAGTCTGGAGACTGGGTCATCACGATGTCCCC13370.1170201778846743No Hit
CCTCTGTTGTGTGCCTGCTGAATAACTTCTATCCCAGAGAGGCCAAAGTA13370.1170201778846743No Hit
CTACTATAACATTGCTGACAGTAATAAACTGCCACATCTTCAGCCTGCAGGCTGCTGATGGTGAGAGTGAAAT13370.1170201778846743No Hit
GACCAGGGTGGAGATCAAACGAACTGTGGCTGCACCATCTGTCTTCATCT13280.11623245791387246No Hit
GGTCTGGGACAGATTTCACTCTCACCATCAGCAGCCTGCAGGCTGAAGAT12970.11351920023666609No Hit
GGGTAGGTCCCCTGTGGAAAAAGGGTCAGAGGCCAAAGGATGGGAGGGGG12880.11273148026586424No Hit
GCACACAACAGAGGCAGTTCCAGATTTCAACTGCTCATCAGATGGCGGGA12840.11238138250106343No Hit
GGTGAAAGATGAGCTGGAGGACCGCAATAGGGGTAGGTCCCCTGTGGAAA12820.11220633361866302No Hit
GTGGAAGGTGGATAACGCCCTCCAATCGGGTAACTCCCAGGAGAGTGTCACAGAGCAGGACAGCAAGGACAGCAC12720.11133108920666096No Hit
CCGCAATAGGGGTAGGTCCCCTGTGGAAAAAGGGTCAGAGGCCAAAGGAT12700.11115604032426055No Hit
GCCCTGGCTTCTGCTGGTACCAAGCAAGGTAGTTCTTATTGTCGGAACTG12450.10896792929425542No Hit
CACCATCAGCAGCCTGCAGGCTGAAGATGTGGCAGTTTATTACTGTCAGCAATGTTATAGTAGTCCGCTCACTTT12370.10826773376465379No Hit
CTACGGGGACATCGTGATGACCCAGTCTCCAGACTCCCTGGCTCTGTCTCTGGGCGAGAGGGCCACCATCAACTG12340.10800516044105317No Hit
AGGTAGTTCTTATTGTCGGAACTGTAGAAAACACTCCGGCTGGACTTGCA12290.10756753823505213No Hit
GAACTGAGGAGCAGGTGGGGGCACTTCTCCCTCTAACACTCTCCCCTGTT12270.10739248935265173No Hit
GTGTTAATACGTGAGACCCATACCAGCCCCTTCCCTGGAGCTTGGCGGAC12120.10607962273464866No Hit
GCTCAGGCCCTGATGGGTGACTTCGCAGGCGTAGACTTTGTGTTTCTCGT12110.10599209829344844No Hit
TCCTTGGGGCTGAAGCCACGTGCCAGGCACGTCAGCGTCACCAGCTCGTT11980.10485428055784579No Hit
GCCTCTGTTGTGTGCCTGCTGAATAACTTCTATCCCAGAGAGGCCAAAGTACAGTGGAAGGTGGATAACGCCCTC11960.10467923167544538No Hit
CTTTGTGACGGGCGAGCTCAGGCCCTGATGGGTGACTTCGCAGGCGTAGA11890.10406656058704394No Hit
GAGGAGGAGGGGGGTGAGGTGAAAGATGAGCTGGAGGACCGCAATAGGGG11880.10397903614584372No Hit
GGCTGAAGATGTGGCAGTTTATTACTGTCAGCAATGTTATAGTAGTCCGC11850.1037164628222431No Hit
CCTCCAACATTAGCATAATTAAAGCCAAGGAGGAGGAGGGGGGTGAGGTG11810.10336636505744229No Hit
GCGTTATCCACCTTCCACTGTACTTTGGCCTCTCTGGGATAGAAGTTATTCAGCAGGCACACAACAGAGGCAGTT11770.10301626729264148No Hit
GGACTGCACACAGAGAACTCACCATGGAGTTTGGGCTGATCTGCGTTTTC11580.10135330290983757No Hit
GTGCTGTCCTTGCTGTCCTGCTCTGTGACACTCTCCTGGGAGTTACCCGATTGGAGGGCGTTATCCACCTTCCA11500.10065310738023593No Hit
GGATAGAAGTTATTCAGCAGGCACACAACAGAGGCAGTTCCAGATTTCAA11480.10047805849783553No Hit

[OK]Adapter Content

Adapter graph

[WARN]Kmer Content

Kmer graph

SequenceCountPValueObs/Exp MaxMax Obs/Exp Position
TCAATCG200.00684173152.71263552
CTAGCGC3300.051.66428828
CGTATGC3350.051.55791541
TCGTATG3300.050.19424440
CGAGACT3400.050.0722923
TAGCGCT3450.049.4440429
TTATACA3550.048.8899962
ATCTCGT3100.048.75267837
GACTAGC3600.048.2595526
CTTATAC3650.047.5776141
ACTAGCG3600.047.3464727
TATACAC3700.046.9079673
ACGAGAC3750.045.39092322
ATGCCGT3900.045.35603744
TACACAT4000.044.242595
AGACTAG3850.044.2351825
CGCTCAT3850.043.5144132
CACGAGA3950.043.09264821
ATCTCCG3950.043.07567610
GGTATCA9600.042.9180031
Produced by FastQC (version 0.11.5)