FastQCFastQC Report
Wed 27 Apr 2022
EGAF00004684427

Summary

[OK]Basic Statistics

MeasureValue
FilenameEGAF00004684427
File typeConventional base calls
EncodingSanger / Illumina 1.9
Total Sequences1142538
Sequences flagged as poor quality0
Sequence length20-76
%GC55

[OK]Per base sequence quality

Per base quality graph

[OK]Per tile sequence quality

Per tile quality graph

[OK]Per sequence quality scores

Per Sequence quality graph

[FAIL]Per base sequence content

Per base sequence content

[FAIL]Per sequence GC content

Per sequence GC content graph

[OK]Per base N content

N content graph

[WARN]Sequence Length Distribution

Sequence length distribution

[FAIL]Sequence Duplication Levels

Duplication level graph

[WARN]Overrepresented sequences

SequenceCountPercentagePossible Source
CTCCTGGGAGTTACCCGATTGGAGGGCGTTATCCACCTTCCACTGTACTTTGGCCTCTCTGGGATAGAAGTTATT51270.44873781003345187No Hit
ATGTTATAGTAGTCCGCTCACTTTCGGCGGAGGGACCAGGGTGGAGATCAAACGAACTGTGGCTGCACCAT42820.3747796572192785No Hit
CTCTAACACTCTCCCCTGTTGAAGCTCTTTGTGACGGGCGAGCTCAGGCC40220.35202330250722513No Hit
GGGGGGGGGGGGGGGGGGGGGGGGGGGGGGGGGGGGGGGGGGGGGGGGGG39940.3495726181536194No Hit
GGCGTAGACTTTGTGTTTCTCGTAGTCTGCTTTGCTCAGCGTCAGGGTGC33140.2900559981374799No Hit
CTCCCAGGAGAGTGTCACAGAGCAGGACAGCAAGGACAGCACCTACAGCC32680.2860298738422704No Hit
GTCTCTGGGCGAGAGGGCCACCATCAACTGCAAGTCCAGCCGGAGTGTTTTCTACAGTTCCGACAATAAGAACTA30690.2686125100434296No Hit
GGCTGGAACTGAGGAGCAGGTGGGGGCACTTCTCCCTCTAACACTCTCCC29360.25697175936380234No Hit
CAACAGAGGCAGTTCCAGATTTCAACTGCTCATCAGATGGCGGGAAGATG28440.24891951077338345No Hit
GTCCTGCTCTGTGACACTCTCCTGGGAGTTACCCGATTGGAGGGCGTTATCCACCTTCCACTGTACTTTGGCCT27810.24340547097777054No Hit
GTACAGTGGAAGGTGGATAACGCCCTCCAATCGGGTAACTCCCAGGAGAG26880.2352656979461515No Hit
CTATAACATTGCTGACAGTAATAAACTGCCACATCTTCAGCCTGCAGGCTGCTGATGGTGAGAGTGAAATCTGTC26680.23351520912214735No Hit
GTCTACGCCTGCGAAGTCACCCATCAGGGCCTGAGCTCGCCCGTCACAAA26200.22931403594453753No Hit
GCGTCAGGGTGCTGCTGAGGCTGTAGGTGCTGTCCTTGCTGTCCTGCTCT25390.2222245562073209No Hit
ACTATAACATTGCTGACAGTAATAAACTGCCACATCTTCAGCCTGCAGGCTGCTGATGGTGAGAGTGAAAT25100.21968634741251497No Hit
GTCTTCATCTTCCCGCCATCTGATGAGCAGTTGAAATCTGGAACTGCCTCTGTTGTGTGCCTGCTGAATAACTT25070.21942377408891434No Hit
GTAATACACAGCCGTGTCCTCGGCCCCCAGACTGTTCATCTGCAGATAAA24430.2138222098521012No Hit
GTAATAAACTGCCACATCTTCAGCCTGCAGGCTGCTGATGGTGAGAGTGAAATCTGTCCCAGACCCGCTGCCA24430.2138222098521012No Hit
GTTTATTACTGTCAGCAATGTTATAGTAGTCCGCTCACTTTCGGCGGAGGGACCAGGGTGGAGATCAAACGAA23340.2042820457612788No Hit
GTGTTGCAGACCCAGGTCTTCATTTCTCTGTTGCTCTGGATCTCTGGTGC23310.2040194724376782No Hit
GGATGGGAGGGGGTCAGGCTGGAACTGAGGAGCAGGTGGGGGCACTTCTCCCTCTAACACTCTCCCCTGTTGAAG23160.20270660581967515No Hit
ATTTAGGAGGCTGCCCTGGCTTCTGCTGGTACCAAGCAAGGTAGTTCTTA23010.20139373920167206No Hit
GTTATAGTAGTCCGCTCACTTTCGGCGGAGGGACCAGGGTGGAGATCAAA22400.19605474828845956No Hit
GTTTTCTACAGTTCCGACAATAAGAACTACCTTGCTTGGTACCAGCAGAAGCCAGGGCAGCCTCCTAAATTGCTC21630.18931536631604376No Hit
CTCTGGGATAGAAGTTATTCAGCAGGCACACAACAGAGGCAGTTCCAGAT20930.1831886554320294No Hit
CCACTGTACTTTGGCCTCTCTGGGATAGAAGTTATTCAGCAGGCACACAACAGAGGCAGTTCCAGATTTCAACTG20650.18073797107842363No Hit
GTGTATTACTGTTCAAGAGGGGATGGGCCGCTCTGGCACCTCAATCTCTGGGGCCGTGGCACCCTGGTCA20580.18012529999002222No Hit
GAGCAGGACAGCAAGGACAGCACCTACAGCCTCAGCAGCACCCTGACGCT20540.1797752022252214No Hit
CTCCTAAATTGCTCCTTTCCTGGGCATCTACCCGGGAATCCGGGGTCCCT20460.17907500669561977No Hit
CAATAGGGGTAGGTCCCCTGTGGAAAAAGGGTCAGAGGCCAAAGGATGGGAGGGGGTCAGGCTGGAACTGAGGAG20160.1764492734596136No Hit
GTGGCTGCACCATCTGTCTTCATCTTCCCGCCATCTGATGAGCAGTTGAAATCTGGAACTGCCTCTGTTGTGTGC20080.17574907793001193No Hit
GGCTGTAGGTGCTGTCCTTGCTGTCCTGCTCTGTGACACTCTCCTGGGAGTTACCCGATTGGAGGGCGTTATCCA19920.17434868687080868No Hit
GTCTCCAGACTCCCTGGCTCTGTCTCTGGGCGAGAGGGCCACCATCAACTGCAAGTCCAGCCGGAGTGTTTTCTA19080.16699663380999144No Hit
GTCCCTGACCGATTCAGTGGCAGCGGGTCTGGGACAGATTTCACTCTCAC19040.1666465360451906No Hit
CCCTGGCTCTGTCTCTGGGCGAGAGGGCCACCATCAACTGCAAGTCCAGC18810.1646334738975859No Hit
GTCCCAGACCCGCTGCCACTGAATCGGTCAGGGACCCCGGATTCCCGGGT18270.15990715407277484No Hit
GAGTGAAATCTGTCCCAGACCCGCTGCCACTGAATCGGTCAGGGACCCCG18070.15815666524877073No Hit
GATTTCACTCTCACCATCAGCAGCCTGCAGGCTGAAGATGTGGCAGTTTATTACTGTCAGCAATGTTATAGTAGT18030.1578065674839699No Hit
GTACCAGCAGAAGCCAGGGCAGCCTCCTAAATTGCTCCTTTCCTGGGCATCTACCCGGGAATCCGGGGTCCCTGA17800.15579350533636516No Hit
ATAGTAGTCCGCTCACTTTCGGCGGAGGGACCAGGGTGGAGATCAAACGA17790.15570598089516496No Hit
GGGTGGAGATCAAACGAACTGTGGCTGCACCATCTGTCTTCATCTTCCCG17750.15535588313036416No Hit
GTGCAGCCACAGTTCGTTTGATCTCCACCCTGGTCCCTCCGCCGAAAGTG17560.15369291874756025No Hit
CCCTGACGCTGAGCAAAGCAGACTACGAGAAACACAAAGTCTACGCCTGCGAAGTCACCCATCAGGGCCTGAGCT17510.15325529654155923No Hit
ATCCCAGAGAGGCCAAAGTACAGTGGAAGGTGGATAACGCCCTCCAATCG16690.1460782923631424No Hit
CTACTATAACATTGCTGACAGTAATAAACTGCCACATCTTCAGCCTGCAGGCTGCTGATGGTGAGAGTGAAAT15840.13863871486112497No Hit
GGCCAAAGGATGGGAGGGGGTCAGGCTGGAACTGAGGAGCAGGTGGGGGCACTTCTCCCTCTAACACTCTCCC15790.13820109265512395No Hit
GTTGTGTGCCTGCTGAATAACTTCTATCCCAGAGAGGCCAAAGTACAGTG15750.13785099489032313No Hit
GACTACTATAACATTGCTGACAGTAATAAACTGCCACATCTTCAGCCTGCAGGCTGCTGATGGTGAGAGTGAAAT15690.13732584824312188No Hit
CCTACAGCCTCAGCAGCACCCTGACGCTGAGCAAAGCAGACTACGAGAAACACAAAGTCTACGCCTGCGAAGTCA15330.13417496835991452No Hit
GTCAGGGACCCCGGATTCCCGGGTAGATGCCCAGGAAAGGAGCAATTTAGGAGGCTGCCCTGGCTTCTGCTGGTA15180.13286210174191143No Hit
GCTTTGCTCAGCGTCAGGGTGCTGCTGAGGCTGTAGGTGCTGTCCTTGCT14940.1307615151531065No Hit
GTACTTTGGCCTCTCTGGGATAGAAGTTATTCAGCAGGCACACAACAGAGGCAGTTCCAGATTTCAACTGCTCAT14730.1289235018879022No Hit
TCTTCAGCCTGCAGGCTGCTGATGGTGAGAGTGAAATCTGTCCCAGACCCGCTGCCACTGAATCGGTCAGGGACC14700.12866092856430159No Hit
CTGCTGGTACCAAGCAAGGTAGTTCTTATTGTCGGAACTGTAGAAAACAC14630.12804825747590012No Hit
GTCCTTGCTGTCCTGCTCTGTGACACTCTCCTGGGAGTTACCCGATTGGAGGGCGTTATCCACCTTCCACTGTA14590.12769815971109932No Hit
CTCCTGTGCAGCCTCTGGATTCACCTTCAGAAGTTACTGGATGCACTGGGTCCGCCAAGCTCCAGGGAAGGGG14580.12761063526989913No Hit
GATCAGGACTGCACACAGAGAACTCACCATGGAGTTTGGGCTGATCTGCG14340.1255100486810942No Hit
GGCTTCTGCTGGTACCAAGCAAGGTAGTTCTTATTGTCGGAACTGTAGAA14180.12410965762189091No Hit
GTAGAAAACACTCCGGCTGGACTTGCAGTTGATGGTGGCCCTCTCGCCCAGAGACAGAGCCAGGGAGTCTGGAGA14160.1239346087394905No Hit
GGCCCTGATGGGTGACTTCGCAGGCGTAGACTTTGTGTTTCTCGTAGTCTGCTTTGCTCAGCGTCAGGGTGCTG13930.12192154659188578No Hit
ATTCAGTGGCAGCGGGTCTGGGACAGATTTCACTCTCACCATCAGCAGCC13680.11973343556188065No Hit
CCTCTGTTGTGTGCCTGCTGAATAACTTCTATCCCAGAGAGGCCAAAGTA13650.11947086223828003No Hit
GGCAGGGGCAGCAAGATGGTGTTGCAGACCCAGGTCTTCATTTCTCTGTT13470.11789542229667634No Hit
GTGTGACCTGGAGCGAAAGCGGACAGAACGTGACCGCCAGAAACTTCCCACCTAGCCAGGATGCCTCCGGGGAC13470.11789542229667634No Hit
CTTCTATCCCAGAGAGGCCAAAGTACAGTGGAAGGTGGATAACGCCCTCCAATCGGGTAACTCCCAGGAGAGTGT13460.11780789785547614No Hit
ACCTTCCACTGTACTTTGGCCTCTCTGGGATAGAAGTTATTCAGCAGGCA13430.11754532453187552No Hit
GAACTACCTTGCTTGGTACCAGCAGAAGCCAGGGCAGCCTCCTAAATTGC13230.11579483570787143No Hit
GATGAAGACAGATGGTGCAGCCACAGTTCGTTTGATCTCCACCCTGGTCC13160.11518216461946999No Hit
GTGTCACAGAGCAGGACAGCAAGGACAGCACCTACAGCCTCAGCAGCACC13100.11465701797226877No Hit
GTTGAAATCTGGAACTGCCTCTGTTGTGTGCCTGCTGAATAACTTCTATC13020.11395682244266711No Hit
CAGTAATAAACTGCCACATCTTCAGCCTGCAGGCTGCTGATGGTGAGAGTGAAATCTGTCCCAGACCCGCTGCCA13000.11378177356026671No Hit
GGATCTCTGGTGCCTACGGGGACATCGTGATGACCCAGTCTCCAGACTCCCTGGCTCTGTCTCTGGGCGAGAGGG12980.1136067246778663No Hit
GTGTACAGGTCCCCGGAGGCATCCTGGCTAGGTGGGAAGTTTCTGGCGGT12930.11316910247186526No Hit
GAGCAAAGCAGACTACGAGAAACACAAAGTCTACGCCTGCGAAGTCACCC12610.1103683203534587No Hit
GACCAGGGTGGAGATCAAACGAACTGTGGCTGCACCATCTGTCTTCATCT12520.10958060038265685No Hit
GTGGAAGGTGGATAACGCCCTCCAATCGGGTAACTCCCAGGAGAGTGTCACAGAGCAGGACAGCAAGGACAGCAC12450.10896792929425542No Hit
GCCTCTGTTGTGTGCCTGCTGAATAACTTCTATCCCAGAGAGGCCAAAGTACAGTGGAAGGTGGATAACGCCCTC12430.10879288041185502No Hit
GGCTGAAGATGTGGCAGTTTATTACTGTCAGCAATGTTATAGTAGTCCGC12330.10791763599985295No Hit
GTCACATCCTGGCTGGGATTCGTGTAGTGCTTCACGTGGCATGTCACGGA12330.10791763599985295No Hit
GTTGAAGCTCTTTGTGACGGGCGAGCTCAGGCCCTGATGGGTGACTTCGC12190.10669229382305008No Hit
GCAGAAGCCAGGGCAGCCTCCTAAATTGCTCCTTTCCTGGGCATCTACCCGGGAATCCGGGGTCCCTGACCGATT12080.10572952496984782No Hit
GGATAGAAGTTATTCAGCAGGCACACAACAGAGGCAGTTCCAGATTTCAA12020.1052043783226466No Hit
GCACACAACAGAGGCAGTTCCAGATTTCAACTGCTCATCAGATGGCGGGA12010.10511685388144638No Hit
CCCCTGTTGAAGCTCTTTGTGACGGGCGAGCTCAGGCCCTGATGGGTGACTTCGCAGGCGTAGACTTTGTGTTT11940.10450418279304495No Hit
GTGTTAATACGTGAGACCCATACCAGCCCCTTCCCTGGAGCTTGGCGGAC11930.10441665835184476No Hit
GCCCTGGCTTCTGCTGGTACCAAGCAAGGTAGTTCTTATTGTCGGAACTG11910.10424160946944434No Hit
GGTCTGGGACAGATTTCACTCTCACCATCAGCAGCCTGCAGGCTGAAGAT11900.10415408502824414No Hit
GTGCTGTCCTTGCTGTCCTGCTCTGTGACACTCTCCTGGGAGTTACCCGATTGGAGGGCGTTATCCACCTTCCA11880.10397903614584372No Hit
AGCCAGGGCAGCCTCCTAAATTGCTCCTTTCCTGGGCATCTACCCGGGAA11850.1037164628222431No Hit
ACCTTGCTTGGTACCAGCAGAAGCCAGGGCAGCCTCCTAAATTGCTCCTT11650.101965973998239No Hit
GTCTGGGACAGATTTCACTCTCACCATCAGCAGCCTGCAGGCTGAAGATG11590.10144082735103778No Hit
GTATCAACGCAGAGTACGGGGAGCTACAACAGGCAGGCAGGGGCAGCAAG11540.10100320514503675No Hit
GCGTTATCCACCTTCCACTGTACTTTGGCCTCTCTGGGATAGAAGTTATTCAGCAGGCACACAACAGAGGCAGTT11500.10065310738023593No Hit

[OK]Adapter Content

Adapter graph

Produced by FastQC (version 0.11.9)