FastQCFastQC Report
Wed 27 Apr 2022
EGAF00004684430

Summary

[OK]Basic Statistics

MeasureValue
FilenameEGAF00004684430
File typeConventional base calls
EncodingSanger / Illumina 1.9
Total Sequences982373
Sequences flagged as poor quality0
Sequence length20-76
%GC54

[OK]Per base sequence quality

Per base quality graph

[OK]Per tile sequence quality

Per tile quality graph

[OK]Per sequence quality scores

Per Sequence quality graph

[FAIL]Per base sequence content

Per base sequence content

[FAIL]Per sequence GC content

Per sequence GC content graph

[OK]Per base N content

N content graph

[WARN]Sequence Length Distribution

Sequence length distribution

[FAIL]Sequence Duplication Levels

Duplication level graph

[WARN]Overrepresented sequences

SequenceCountPercentagePossible Source
CTCCTGGGAGTTACCCGATTGGAGGGCGTTATCCACCTTCCACTGTACTTTGGCCTCTCTGGGATAGAAGTTATT41630.4237697900899149No Hit
CTCTAACACTCTCCCCTGTTGAAGCTCTTTGTGACGGGCGAGCTCAGGCC39310.40015350584757514No Hit
CCCCTGGAGAGCCGGCCTCCATCTCCTGCAGGTCTAGTCAGAGCCTCCTG33940.3454899513728492No Hit
GGCTGGAACTGAGGAGCAGGTGGGGGCACTTCTCCCTCTAACACTCTCCC28010.28512591449479985No Hit
GGCGTAGACTTTGTGTTTCTCGTAGTCTGCTTTGCTCAGCGTCAGGGTGC27770.28268285060766124No Hit
GGTCTAGTCAGAGCCTCCTGTTTAGTAGTGGAAACAACCACTTGGATTGGTACCTGCAGAAGCCAGGGCAGTCTC27610.28105414134956885No Hit
GTAATACACAGCCGTGTCCTCGGCTCTCAGACTGTTCATTTGCAGATACA26870.27352136103089153No Hit
GTAATAAACCCCAACATCCTCAGCCTCCACTCTACTGATTTTCAGTGTAAAATCTGTGCCTGATCCACTGCCA25840.26303654518192177No Hit
GTTTAGTAGTGGAAACAACCACTTGGATTGGTACCTGCAGAAGCCAGGGC25620.2607970699520447No Hit
CAACAGAGGCAGTTCCAGATTTCAACTGCTCATCAGATGGCGGGAAGATG25460.25916836069395227No Hit
GGACTCCTCAGTTCACCTTCTCACAATGAGGCTCCCTGCTCAGCTCCTGG25440.25896477203669077No Hit
GATCAGGACTCCTCAGTTCACCTTCTCACAATGAGGCTCCCTGCTCAGCTCCTGGGGCTGCTAATGCTCTGGGT23690.2411507645263052No Hit
CTCCCAGGAGAGTGTCACAGAGCAGGACAGCAAGGACAGCACCTACAGCC23560.2398274382541051No Hit
GGATGGGAGGGGGTCAGGCTGGAACTGAGGAGCAGGTGGGGGCACTTCTCCCTCTAACACTCTCCCCTGTTGAAG23480.2390130836250589No Hit
GGGCTATTGTGATGACTCAGTCTCCACTCTTTTTGCCCGTCACCCCTGGAGAGCCGGCCTCCATCTCCTGCAGGT22960.2337197785362586No Hit
GTCCTGCTCTGTGACACTCTCCTGGGAGTTACCCGATTGGAGGGCGTTATCCACCTTCCACTGTACTTTGGCCT22870.23280362957858167No Hit
CAATAGGGGTAGGTCCCCTGTGGAAAAAGGGTCAGAGGCCAAAGGATGGGAGGGGGTCAGGCTGGAACTGAGGAG22840.2324982465926893No Hit
GTCTACGCCTGCGAAGTCACCCATCAGGGCCTGAGCTCGCCCGTCACAAA22340.22740853016115062No Hit
GTACAGTGGAAGGTGGATAACGCCCTCCAATCGGGTAACTCCCAGGAGAG22150.22547443791716587No Hit
GCGTCAGGGTGCTGCTGAGGCTGTAGGTGCTGTCCTTGCTGTCCTGCTCT21680.2206901044715195No Hit
GTGTATTACTGTGCGAGATTCATGTGTCTCCAGCCTGGTAGCAGCACCTG21480.218654217898904No Hit
CCACTGTACTTTGGCCTCTCTGGGATAGAAGTTATTCAGCAGGCACACAACAGAGGCAGTTCCAGATTTCAACTG18880.19218769245490258No Hit
CTTCAAACTCCCCTCACTTTCGGCGGGGGGACCAAGGTGGAGATCAAACG18550.188828479610087No Hit
CTCTGGGATAGAAGTTATTCAGCAGGCACACAACAGAGGCAGTTCCAGAT18410.18740335900925617No Hit
GTCTTCATCTTCCCGCCATCTGATGAGCAGTTGAAATCTGGAACTGCCTCTGTTGTGTGCCTGCTGAATAACTT18390.18719977035199462No Hit
CAATGAGGCTCCCTGCTCAGCTCCTGGGGCTGCTAATGCTCTGGGTCTCTGGATCCCGTGGGGCTATTGTGATGA17950.18272081989224054No Hit
GTGCAGCCACAGTTCGTTTGATCTCCACCTTGGTCCCCCCGCCGAAAGTG16870.17172703240011686No Hit
GATTTTACACTGAAAATCAGTAGAGTGGAGGCTGAGGATGTTGGGGTTTATTACTGCATGCAAGCTCTTCAAACT16770.1707090891138091No Hit
GGCTGTAGGTGCTGTCCTTGCTGTCCTGCTCTGTGACACTCTCCTGGGAGTTACCCGATTGGAGGGCGTTATCCA16730.170301911799286No Hit
GATGAAGACAGATGGTGCAGCCACAGTTCGTTTGATCTCCACCTTGGTCC16660.16958935149887058No Hit
CCTCAGTTCACCTTCTCACAATGAGGCTCCCTGCTCAGCTCCTGGGGCTG16550.16846961388393208No Hit
GCTAATGCTCTGGGTCTCTGGATCCCGTGGGGCTATTGTGATGACTCAGTCTCCACTCTTTTTGCCCGTCACCC16540.16836781955530128No Hit
GTGGCTGCACCATCTGTCTTCATCTTCCCGCCATCTGATGAGCAGTTGAAATCTGGAACTGCCTCTGTTGTGTGC16350.1664337273113166No Hit
GTGTAAAATCTGTGCCTGATCCACTGCCACTGAACCTGTCAGGGACCCCG16290.16582296133953192No Hit
CTTATACACATCTCCGAGCCCACGAGACCCTAAGACATCTCGTATGCCGT16250.16541578402500884No Hit
AGCCAGGGCAGTCTCCACAGCTCCTGATCTATTTGGCTTCTAGTCGGGCC16040.16327810312376256No Hit
CTCCAGGGGTGACGGGCAAAAAGAGTGGAGACTGAGTCATCACAATAGCC15980.1626673371519779No Hit
GGCCAAAGGATGGGAGGGGGTCAGGCTGGAACTGAGGAGCAGGTGGGGGCACTTCTCCCTCTAACACTCTCCC15930.16215836550882404No Hit
GCTCCTGGGGCTGCTAATGCTCTGGGTCTCTGGATCCCGTGGGGCTATTG15580.15859556400674693No Hit
GTCAGGGACCCCGGGGGCCCGACTAGAAGCCAAATAGATCAGGAGCTGTGGAGACTGCCCTGGCTTCTGCAGGTA15550.15829018102085463No Hit
GTCCCTGACAGGTTCAGTGGCAGTGGATCAGGCACAGATTTTACACTGAA15470.1574758263918084No Hit
ATCCCAGAGAGGCCAAAGTACAGTGGAAGGTGGATAACGCCCTCCAATCG15270.15543993981919293No Hit
GTATCAACGCAGAGTACGGGGATCAGGACTCCTCAGTTCACCTTCTCACA14490.14749998218599247No Hit
CTACTAAACAGGAGGCTCTGACTAGACCTGCAGGAGATGGAGGCCGGCTC14110.14363179769802306No Hit
GCTCAGCTCCTGGGGCTGCTAATGCTCTGGGTCTCTGGATCCCGTGGGGCTATTGTGATGACTCAGTCTCCACT14100.14353000336939226No Hit
GGCCCTGATGGGTGACTTCGCAGGCGTAGACTTTGTGTTTCTCGTAGTCTGCTTTGCTCAGCGTCAGGGTGCTG14010.1426138544117153No Hit
GACTAGACCTGCAGGAGATGGAGGCCGGCTCTCCAGGGGTGACGGGCAAA13970.1422066770971922No Hit
GTACTTTGGCCTCTCTGGGATAGAAGTTATTCAGCAGGCACACAACAGAGGCAGTTCCAGATTTCAACTGCTCAT13840.14088335082499212No Hit
GAGCAGGACAGCAAGGACAGCACCTACAGCCTCAGCAGCACCCTGACGCT13630.13874566992374587No Hit
GCTTTGCTCAGCGTCAGGGTGCTGCTGAGGCTGTAGGTGCTGTCCTTGCT13460.1370151663370227No Hit
GTTCAGTGGCAGTGGATCAGGCACAGATTTTACACTGAAAATCAGTAGAG13430.13670978335113038No Hit
GTTGTGTGCCTGCTGAATAACTTCTATCCCAGAGAGGCCAAAGTACAGTG13250.13487748543577643No Hit
GAGTTTGAAGAGCTTGCATGCAGTAATAAACCCCAACATCCTCAGCCTCCACTCTACTGATTTTCAGTGTAAAAT13150.13385954214946869No Hit
GTTGAAGCTCTTTGTGACGGGCGAGCTCAGGCCCTGATGGGTGACTTCGC12900.13131468393369933No Hit
GCCCTGGCTTCTGCAGGTACCAATCCAAGTGGTTGTTTCCACTACTAAAC12900.13131468393369933No Hit
GTTTATTACTGCATGCAAGCTCTTCAAACTCCCCTCACTTTCGGCGGGGGGACCAAGGTGGAGATCAAACGAA12850.13080571229054544No Hit
CAGTAATAAACCCCAACATCCTCAGCCTCCACTCTACTGATTTTCAGTGTAAAATCTGTGCCTGATCCACTGCCA12800.13029674064739155No Hit
CTTTTTGCCCGTCACCCCTGGAGAGCCGGCCTCCATCTCCTGCAGGTCTA12760.12988956333286847No Hit
GTCACATCCTGGCTGGGATTCGTGTAGTGCTTCACGTGGCATGTCACGGA12580.12805726541751453No Hit
GTCCTTGCTGTCCTGCTCTGTGACACTCTCCTGGGAGTTACCCGATTGGAGGGCGTTATCCACCTTCCACTGTA12370.12591958451626825No Hit
CCTTCTCACAATGAGGCTCCCTGCTCAGCTCCTGGGGCTGCTAATGCTCT12250.12469805257269898No Hit
GTATGCTGGTCACAGCGAAGGTGGTGGTGCCCTGGCTGGGCTCCTGCCGG12180.12398549227228355No Hit
TCCTTGGGGCTGAAGCCACGTGCCAGGCACGTCAGCGTCACCAGCTCGTT11800.1201173077843141No Hit
CTCCTGTGCAGCGTCTGGATTCACCTTCAGTAATTATGGCATGCACTGGGTCCGCCAGGCTCCAGGCAAGGGG11780.11991371912705255No Hit
GTTCACCTTCTCACAATGAGGCTCCCTGCTCAGCTCCTGGGGCTGCTAAT11700.11909936449800634No Hit
GGGTCTCTGGATCCCGTGGGGCTATTGTGATGACTCAGTCTCCACTCTTTTTGCCCGTCACCCCTGGAGAGCCGG11670.11879398151211404No Hit
GGGTAGGTCCCCTGTGGAAAAAGGGTCAGAGGCCAAAGGATGGGAGGGGG11380.11584194598182158No Hit
GTGGAAAAAGGGTCAGAGGCCAAAGGATGGGAGGGGGTCAGGCTGGAACTGAGGAGCAGGTGGGGGCACTTCTCC11330.1153329743386677No Hit
GTGTACAGGTCCCCGGAGGCATCCTGGCTAGGTGGGAAGTTTCTGGCGGT11230.11431503105235996No Hit
GCACTGAACACAGAGGACTCACCATGGAGTTTGGGCTGAGCTGGGTTTTC11230.11431503105235996No Hit
GTCTCCACTCTTTTTGCCCGTCACCCCTGGAGAGCCGGCCTCCATCTCCT11210.1141114423950984No Hit
CTTCTATCCCAGAGAGGCCAAAGTACAGTGGAAGGTGGATAACGCCCTCCAATCGGGTAACTCCCAGGAGAGTGT11200.11400964806646761No Hit
GTTGAAATCTGGAACTGCCTCTGTTGTGTGCCTGCTGAATAACTTCTATC11190.11390785373783685No Hit
CCGCAATAGGGGTAGGTCCCCTGTGGAAAAAGGGTCAGAGGCCAAAGGAT11180.11380605940920607No Hit
TTATTACTGCATGCAAGCTCTTCAAACTCCCCTCACTTTCGGCGGGGGGA11140.11339888209468296No Hit
GTATTACTGTGCGAGATTCATGTGTCTCCAGCCTGGTAGCAGCACCTGTCATTCCCCCTATTACGCTATCGACGG11130.1132970877660522No Hit
GCACACAACAGAGGCAGTTCCAGATTTCAACTGCTCATCAGATGGCGGGA10950.11146478985069827No Hit
ACCTTCCACTGTACTTTGGCCTCTCTGGGATAGAAGTTATTCAGCAGGCA10790.10983608059260586No Hit
GTGCAGCGACAGTCGGGGGTGGCAGCATGGGGGAGGTGGGGGAACTGGGC10750.10942890327808276No Hit
CCCCTGTTGAAGCTCTTTGTGACGGGCGAGCTCAGGCCCTGATGGGTGACTTCGCAGGCGTAGACTTTGTGTTT10690.10881813730629812No Hit
GTGGAAGGTGGATAACGCCCTCCAATCGGGTAACTCCCAGGAGAGTGTCACAGAGCAGGACAGCAAGGACAGCAC10570.10759660536272882No Hit
GGTGAAAGATGAGCTGGAGGACCGCAATAGGGGTAGGTCCCCTGTGGAAA10530.10718942804820572No Hit
CCTCTGTTGTGTGCCTGCTGAATAACTTCTATCCCAGAGAGGCCAAAGTA10420.1060696904332672No Hit
CAGTAATACACAGCCGTGTCCTCGGCTCTCAGACTGTTCATTTGCAGATA10360.10545892446148254No Hit
GCAGAAGCCAGGGCAGTCTCCACAGCTCCTGATCTATTTGGCTTCTAGTCGGGCCCCCGGGGTCCCTGACAGGTT10330.10515354147559022No Hit
GTGCTGTCCTTGCTGTCCTGCTCTGTGACACTCTCCTGGGAGTTACCCGATTGGAGGGCGTTATCCACCTTCCA10260.10444098117517481No Hit
GAACTGAGGAGCAGGTGGGGGCACTTCTCCCTCTAACACTCTCCCCTGTT10240.10423739251791327No Hit
AGTCAGAGCCTCCTGTTTAGTAGTGGAAACAACCACTTGGATTGGTACCTGCAGAAGCCAGGGCAGTCTCCACAG10220.10403380386065171No Hit
GTTCAAGGACCACCTGAGCGTGACCTCTGTGGCTGCTACAGCGTGTCCAG10150.1033212435602363No Hit
TCCCTGCTCAGCTCCTGGGGCTGCTAATGCTCTGGGTCTCTGGATCCCGTGGGGCTATTGTGATGACTCAGTCTC10110.10291406624571318No Hit
GAGCAAAGCAGACTACGAGAAACACAAAGTCTACGCCTGCGAAGTCACCC10010.10189612295940544No Hit
CCTCCAACATTAGCATAATTAAAGCCAAGGAGGAGGAGGGGGGTGAGGTG10010.10189612295940544No Hit
GTGTGCACGTGAGGTTCGCTTCTGAACCTAAGAGCAGGTCCTCGAGGGCC9970.10148894564488234No Hit
GCCTCTGTTGTGTGCCTGCTGAATAACTTCTATCCCAGAGAGGCCAAAGTACAGTGGAAGGTGGATAACGCCCTC9970.10148894564488234No Hit
GGATAGAAGTTATTCAGCAGGCACACAACAGAGGCAGTTCCAGATTTCAA9970.10148894564488234No Hit
GCTCAGGCCCTGATGGGTGACTTCGCAGGCGTAGACTTTGTGTTTCTCGT9950.10128535698762078No Hit
GCGTTATCCACCTTCCACTGTACTTTGGCCTCTCTGGGATAGAAGTTATTCAGCAGGCACACAACAGAGGCAGTT9930.10108176833035923No Hit

[OK]Adapter Content

Adapter graph

Produced by FastQC (version 0.11.9)