FastQCFastQC Report
Wed 27 Apr 2022
EGAF00004684431

Summary

[OK]Basic Statistics

MeasureValue
FilenameEGAF00004684431
File typeConventional base calls
EncodingSanger / Illumina 1.9
Total Sequences982373
Sequences flagged as poor quality0
Sequence length20-76
%GC55

[OK]Per base sequence quality

Per base quality graph

[OK]Per tile sequence quality

Per tile quality graph

[OK]Per sequence quality scores

Per Sequence quality graph

[FAIL]Per base sequence content

Per base sequence content

[FAIL]Per sequence GC content

Per sequence GC content graph

[OK]Per base N content

N content graph

[WARN]Sequence Length Distribution

Sequence length distribution

[FAIL]Sequence Duplication Levels

Duplication level graph

[WARN]Overrepresented sequences

SequenceCountPercentagePossible Source
CTCCTGGGAGTTACCCGATTGGAGGGCGTTATCCACCTTCCACTGTACTTTGGCCTCTCTGGGATAGAAGTTATT40820.41552444947082223No Hit
GGGGGGGGGGGGGGGGGGGGGGGGGGGGGGGGGGGGGGGGGGGGGGGGGG35000.3562801502077113No Hit
CTCTAACACTCTCCCCTGTTGAAGCTCTTTGTGACGGGCGAGCTCAGGCC31630.32197546145914024No Hit
CCCCTGGAGAGCCGGCCTCCATCTCCTGCAGGTCTAGTCAGAGCCTCCTG30990.31546062442677064No Hit
GGCGTAGACTTTGTGTTTCTCGTAGTCTGCTTTGCTCAGCGTCAGGGTGC27790.2828864392649228No Hit
CTCCCAGGAGAGTGTCACAGAGCAGGACAGCAAGGACAGCACCTACAGCC25980.2644616657827526No Hit
GGTCTAGTCAGAGCCTCCTGTTTAGTAGTGGAAACAACCACTTGGATTGGTACCTGCAGAAGCCAGGGCAGTCTC25630.26089886428067544No Hit
GTAATACACAGCCGTGTCCTCGGCTCTCAGACTGTTCATTTGCAGATACA25290.25743785710722916No Hit
GTAATAAACCCCAACATCCTCAGCCTCCACTCTACTGATTTTCAGTGTAAAATCTGTGCCTGATCCACTGCCA24640.2508212257462288No Hit
GTTTAGTAGTGGAAACAACCACTTGGATTGGTACCTGCAGAAGCCAGGGC24480.24919251648813637No Hit
GATCAGGACTCCTCAGTTCACCTTCTCACAATGAGGCTCCCTGCTCAGCTCCTGGGGCTGCTAATGCTCTGGGT24340.24776739588730554No Hit
CAACAGAGGCAGTTCCAGATTTCAACTGCTCATCAGATGGCGGGAAGATG23750.24176153049808982No Hit
GGGCTATTGTGATGACTCAGTCTCCACTCTTTTTGCCCGTCACCCCTGGAGAGCCGGCCTCCATCTCCTGCAGGT22770.2317856862922739No Hit
GTCCTGCTCTGTGACACTCTCCTGGGAGTTACCCGATTGGAGGGCGTTATCCACCTTCCACTGTACTTTGGCCT22570.22974979971965842No Hit
GGCTGGAACTGAGGAGCAGGTGGGGGCACTTCTCCCTCTAACACTCTCCC22340.22740853016115062No Hit
GGACTCCTCAGTTCACCTTCTCACAATGAGGCTCCCTGCTCAGCTCCTGG22330.2273067358325198No Hit
GTGTATTACTGTGCGAGATTCATGTGTCTCCAGCCTGGTAGCAGCACCTG22160.22557623224579668No Hit
GTACAGTGGAAGGTGGATAACGCCCTCCAATCGGGTAACTCCCAGGAGAG20920.2129537354955806No Hit
GCGTCAGGGTGCTGCTGAGGCTGTAGGTGCTGTCCTTGCTGTCCTGCTCT19830.20185815367482615No Hit
GTCTACGCCTGCGAAGTCACCCATCAGGGCCTGAGCTCGCCCGTCACAAA19690.20043303307399532No Hit
GTCTTCATCTTCCCGCCATCTGATGAGCAGTTGAAATCTGGAACTGCCTCTGTTGTGTGCCTGCTGAATAACTT18790.19127154349722558No Hit
CTTCAAACTCCCCTCACTTTCGGCGGGGGGACCAAGGTGGAGATCAAACG18440.18770874199514848No Hit
GGATGGGAGGGGGTCAGGCTGGAACTGAGGAGCAGGTGGGGGCACTTCTCCCTCTAACACTCTCCCCTGTTGAAG18320.1864872100515792No Hit
GTGGCTGCACCATCTGTCTTCATCTTCCCGCCATCTGATGAGCAGTTGAAATCTGGAACTGCCTCTGTTGTGTGC17340.17651136584576327No Hit
CTCTGGGATAGAAGTTATTCAGCAGGCACACAACAGAGGCAGTTCCAGAT17320.1763077771885017No Hit
CAATAGGGGTAGGTCCCCTGTGGAAAAAGGGTCAGAGGCCAAAGGATGGGAGGGGGTCAGGCTGGAACTGAGGAG17080.17386471330136313No Hit
CCTCAGTTCACCTTCTCACAATGAGGCTCCCTGCTCAGCTCCTGGGGCTG17010.17315215300094772No Hit
CCACTGTACTTTGGCCTCTCTGGGATAGAAGTTATTCAGCAGGCACACAACAGAGGCAGTTCCAGATTTCAACTG16950.17254138702916305No Hit
GATTTTACACTGAAAATCAGTAGAGTGGAGGCTGAGGATGTTGGGGTTTATTACTGCATGCAAGCTCTTCAAACT16860.17162523807148608No Hit
CAATGAGGCTCCCTGCTCAGCTCCTGGGGCTGCTAATGCTCTGGGTCTCTGGATCCCGTGGGGCTATTGTGATGA16710.17009832314202447No Hit
GGCTGTAGGTGCTGTCCTTGCTGTCCTGCTCTGTGACACTCTCCTGGGAGTTACCCGATTGGAGGGCGTTATCCA16630.16928396851297828No Hit
GTGCAGCCACAGTTCGTTTGATCTCCACCTTGGTCCCCCCGCCGAAAGTG16430.16724808194036278No Hit
GTGTAAAATCTGTGCCTGATCCACTGCCACTGAACCTGTCAGGGACCCCG16350.1664337273113166No Hit
GAGCAGGACAGCAAGGACAGCACCTACAGCCTCAGCAGCACCCTGACGCT16290.16582296133953192No Hit
GATGAAGACAGATGGTGCAGCCACAGTTCGTTTGATCTCCACCTTGGTCC15490.15767941504906996No Hit
GCTCCTGGGGCTGCTAATGCTCTGGGTCTCTGGATCCCGTGGGGCTATTG15270.15543993981919293No Hit
GTATCAACGCAGAGTACGGGGATCAGGACTCCTCAGTTCACCTTCTCACA15090.15360764190383897No Hit
AGCCAGGGCAGTCTCCACAGCTCCTGATCTATTTGGCTTCTAGTCGGGCC14990.15258969861753124No Hit
GCTAATGCTCTGGGTCTCTGGATCCCGTGGGGCTATTGTGATGACTCAGTCTCCACTCTTTTTGCCCGTCACCC14860.15126637234533116No Hit
GTCAGGGACCCCGGGGGCCCGACTAGAAGCCAAATAGATCAGGAGCTGTGGAGACTGCCCTGGCTTCTGCAGGTA14780.15045201771628497No Hit
CTCCAGGGGTGACGGGCAAAAAGAGTGGAGACTGAGTCATCACAATAGCC14750.15014663473039264No Hit
CCCTGACGCTGAGCAAAGCAGACTACGAGAAACACAAAGTCTACGCCTGCGAAGTCACCCATCAGGGCCTGAGCT14630.14892510278682333No Hit
GTCCCTGACAGGTTCAGTGGCAGTGGATCAGGCACAGATTTTACACTGAA14100.14353000336939226No Hit
GAGTTTGAAGAGCTTGCATGCAGTAATAAACCCCAACATCCTCAGCCTCCACTCTACTGATTTTCAGTGTAAAAT13960.14210488276856142No Hit
GTTCAGTGGCAGTGGATCAGGCACAGATTTTACACTGAAAATCAGTAGAG13510.13752413798017657No Hit
CTCCAGCCCCTTGCCTGGAGCCTGGCGGACCCAGTGCATGCCATAATTAC13490.13732054932291501No Hit
GCTCAGCTCCTGGGGCTGCTAATGCTCTGGGTCTCTGGATCCCGTGGGGCTATTGTGATGACTCAGTCTCCACT13240.13477569110714566No Hit
GTTGTGTGCCTGCTGAATAACTTCTATCCCAGAGAGGCCAAAGTACAGTG13240.13477569110714566No Hit
CTACTAAACAGGAGGCTCTGACTAGACCTGCAGGAGATGGAGGCCGGCTC13110.1334523648349456No Hit
CAGTAATAAACCCCAACATCCTCAGCCTCCACTCTACTGATTTTCAGTGTAAAATCTGTGCCTGATCCACTGCCA12870.131009300947807No Hit
GTTTATTACTGCATGCAAGCTCTTCAAACTCCCCTCACTTTCGGCGGGGGGACCAAGGTGGAGATCAAACGAA12760.12988956333286847No Hit
GTCCTTGCTGTCCTGCTCTGTGACACTCTCCTGGGAGTTACCCGATTGGAGGGCGTTATCCACCTTCCACTGTA12720.12948238601834539No Hit
ATCCCAGAGAGGCCAAAGTACAGTGGAAGGTGGATAACGCCCTCCAATCG12690.12917700303245305No Hit
GACTAGACCTGCAGGAGATGGAGGCCGGCTCTCCAGGGGTGACGGGCAAA12510.1273447051170991No Hit
GGGTCTCTGGATCCCGTGGGGCTATTGTGATGACTCAGTCTCCACTCTTTTTGCCCGTCACCCCTGGAGAGCCGG12370.12591958451626825No Hit
GTGTGACCTGGAGCGAAAGCGGACAGAACGTGACCGCCAGAAACTTCCCACCTAGCCAGGATGCCTCCGGGGAC12330.12551240720174517No Hit
CCTACAGCCTCAGCAGCACCCTGACGCTGAGCAAAGCAGACTACGAGAAACACAAAGTCTACGCCTGCGAAGTCA12320.1254106128731144No Hit
GTACTTTGGCCTCTCTGGGATAGAAGTTATTCAGCAGGCACACAACAGAGGCAGTTCCAGATTTCAACTGCTCAT12260.12479984690132974No Hit
GCTTTGCTCAGCGTCAGGGTGCTGCTGAGGCTGTAGGTGCTGTCCTTGCT12180.12398549227228355No Hit
GTTCACCTTCTCACAATGAGGCTCCCTGCTCAGCTCCTGGGGCTGCTAAT12140.12357831495776045No Hit
CTTTTTGCCCGTCACCCCTGGAGAGCCGGCCTCCATCTCCTGCAGGTCTA12050.12266216600008348No Hit
GGCCCTGATGGGTGACTTCGCAGGCGTAGACTTTGTGTTTCTCGTAGTCTGCTTTGCTCAGCGTCAGGGTGCTG11990.12205140002829883No Hit
GGCCAAAGGATGGGAGGGGGTCAGGCTGGAACTGAGGAGCAGGTGGGGGCACTTCTCCCTCTAACACTCTCCC11860.12072807375609876No Hit
TTATTACTGCATGCAAGCTCTTCAAACTCCCCTCACTTTCGGCGGGGGGA11790.12001551345568334No Hit
CCTTCTCACAATGAGGCTCCCTGCTCAGCTCCTGGGGCTGCTAATGCTCT11600.11808142121169862No Hit
GTCTCCACTCTTTTTGCCCGTCACCCCTGGAGAGCCGGCCTCCATCTCCT11570.11777603822580628No Hit
GCCCTGGCTTCTGCAGGTACCAATCCAAGTGGTTGTTTCCACTACTAAAC11570.11777603822580628No Hit
ACCTTCCACTGTACTTTGGCCTCTCTGGGATAGAAGTTATTCAGCAGGCA11420.11624912329634465No Hit
CTTCTATCCCAGAGAGGCCAAAGTACAGTGGAAGGTGGATAACGCCCTCCAATCGGGTAACTCCCAGGAGAGTGT11060.11258452746563678No Hit
GTGTCACAGAGCAGGACAGCAAGGACAGCACCTACAGCCTCAGCAGCACC11060.11258452746563678No Hit
CCTCTGTTGTGTGCCTGCTGAATAACTTCTATCCCAGAGAGGCCAAAGTA10960.11156658417932903No Hit
GTTGAAATCTGGAACTGCCTCTGTTGTGTGCCTGCTGAATAACTTCTATC10820.11014146357849819No Hit
GTTCAAGGACCACCTGAGCGTGACCTCTGTGGCTGCTACAGCGTGTCCAG10820.11014146357849819No Hit
AGTCAGAGCCTCCTGTTTAGTAGTGGAAACAACCACTTGGATTGGTACCTGCAGAAGCCAGGGCAGTCTCCACAG10450.10637507341915954No Hit
GTTGAAGCTCTTTGTGACGGGCGAGCTCAGGCCCTGATGGGTGACTTCGC10380.1056625131187441No Hit
CAGTAATACACAGCCGTGTCCTCGGCTCTCAGACTGTTCATTTGCAGATA10320.10505174714695946No Hit
CCCCTGTTGAAGCTCTTTGTGACGGGCGAGCTCAGGCCCTGATGGGTGACTTCGCAGGCGTAGACTTTGTGTTT10310.10494995281832868No Hit
CTCCTGTGCAGCGTCTGGATTCACCTTCAGTAATTATGGCATGCACTGGGTCCGCCAGGCTCCAGGCAAGGGG10290.10474636416106713No Hit
GAGCAAAGCAGACTACGAGAAACACAAAGTCTACGCCTGCGAAGTCACCC10190.10372842087475939No Hit
GCCTCTGTTGTGTGCCTGCTGAATAACTTCTATCCCAGAGAGGCCAAAGTACAGTGGAAGGTGGATAACGCCCTC10110.10291406624571318No Hit
GTGGAAGGTGGATAACGCCCTCCAATCGGGTAACTCCCAGGAGAGTGTCACAGAGCAGGACAGCAAGGACAGCAC10060.1024050946025593No Hit
GTATTACTGTGCGAGATTCATGTGTCTCCAGCCTGGTAGCAGCACCTGTCATTCCCCCTATTACGCTATCGACGG9930.10108176833035923No Hit
GCACACAACAGAGGCAGTTCCAGATTTCAACTGCTCATCAGATGGCGGGA9910.1008781796730977No Hit
GTGTACAGGTCCCCGGAGGCATCCTGGCTAGGTGGGAAGTTTCTGGCGGT9850.10026741370131305No Hit

[OK]Adapter Content

Adapter graph

Produced by FastQC (version 0.11.9)