FastQCFastQC Report
Sat 23 Apr 2022
EGAF00004684434

Summary

[OK]Basic Statistics

MeasureValue
FilenameEGAF00004684434
File typeConventional base calls
EncodingSanger / Illumina 1.9
Total Sequences1541925
Sequences flagged as poor quality0
Sequence length20-76
%GC57

[OK]Per base sequence quality

Per base quality graph

[OK]Per tile sequence quality

Per base quality graph

[OK]Per sequence quality scores

Per Sequence quality graph

[FAIL]Per base sequence content

Per base sequence content

[FAIL]Per sequence GC content

Per sequence GC content graph

[OK]Per base N content

N content graph

[WARN]Sequence Length Distribution

Sequence length distribution

[FAIL]Sequence Duplication Levels

Duplication level graph

[WARN]Overrepresented sequences

SequenceCountPercentagePossible Source
GGTTAATAGTGATGGCAGCCACGACAAGGGGGACGGGGTCCCTGATCGCT72890.47272078732752887No Hit
GCCCAAGGCTGCCCCCTCGGTCACTCTGTTCCCGCCCTCCTCTGAGGAGC58810.3814063589344488No Hit
GCCTTGGGCTGACCTAGGACGGTCAGCTTGGTCCCTCCGCCGAACACCCA55990.3631175316568575No Hit
CTATTAACCTTCATCAAATATCGAGGGCCCTTCTCTGGAAGCTGCTGATGCCAAGCGATGACGTAGGTGCTGTAG55190.35792921186179616No Hit
GACCTAGGACGGTCAGCTTGGTCCCTCCGCCGAACACCCAATTGCCAGTGCCCCAGGTCTGACAGTAATAGTCAC44700.28989736854905396No Hit
GTGTGGTGGTCTCCACTCCCGCCTTGACGGGGCTGCTATCTGCCTTCCAG42930.27841821100248065No Hit
CCTCTGAGGAGCTTCAAGCCAACAAGGCCACACTGGTGTGTCTCATAAGT40940.26551226551226553No Hit
GGGTCTCTCTCCCAGCTTGCGGTGACTCAATCGCCCTCTGCCTCTGCCTCCCTGGGAGCCTCGGTCAAGCTCAC40500.26265868962498173No Hit
GCTCTGGGGAGTCTGCACCATGGCTTGGACCCCACTCCTCTTCCTCACCCTCCTCCTCCACTGCACAGGGTCTCT39120.25370883797850086No Hit
CCTCAGAGGAGGGCGGGAACAGAGTGACCGAGGGGGCAGCCTTGGGCTGACCTAGGACGGTCAGCTTGGTCCCTC38830.2518280720527912No Hit
TCCTAGGTCAGCCCAAGGCTGCCCCCTCGGTCACTCTGTTCCCGCCCTCCTCTGAGGAGCTTCAAGCCAACAAGG36680.23788446260356372No Hit
CTTATACACATCTCCGAGCCCACGAGACTAAGGCGAATCTCGTATGCCGT35640.23113964686998395No Hit
GCCCCAGAGCTGGAGCCTGAGAAGCGATCAGGGACCCCGTCCCCCTTGTC32160.208570455761467No Hit
GTAATACACAGCCGTGTCCTCGGCTCTCAGGCTGTTCATTTGCAGATACA31970.20733822981013994No Hit
CCTATGAACATTCTGTAGGGGCCACTGTCTTCTCCACGGTGCTCCCTTCA31560.20467921591517096No Hit
CTCTATGAGTGGCCGCTTCACCATCTCCAGAGAAAACGCCAACAACGCAC31430.20383611394847348No Hit
GTCTTCTCCACGGTGCTCCCTTCATGCGTGACCTGGCAGCTGTAGCTTTT31020.20117710005350453No Hit
GTGCAGGGAGAAGGGCTTGATGCCTTGGGGTGGGAGGAGAGACCCCTCCCCTGGGATCCTGCAGCTCTAGTCTCC30850.20007458209705403No Hit
CTATTACTGTCAGACCTGGGGCACTGGCAATTGGGTGTTCGGCGGAGGGACCAAGCTGACCGTCCTAGGTCAGCC30370.1969615902200172No Hit
CCTCCTCCACTGCACAGGGTCTCTCTCCCAGCTTGCGGTGACTCAATCGCCCTCTGCCTCTGCCTCCCTGGGAGC30220.19598878025844316No Hit
GTCCTAGGTCAGCCCAAGGCTGCCCCCTCGGTCACTCTGTTCCCGCCCTC30020.19469170030967783No Hit
GTGCTCCCTTCATGCGTGACCTGGCAGCTGTAGCTTTTGTGGGACTTCCACTGCTCAGGCGTCAGGCTCAGGTAG28720.18626068064270312No Hit
GTTTGGAGGGTGTGGTGGTCTCCACTCCCGCCTTGACGGGGCTGCTATCTGCCTTCCAGGCCACTGTCACGGCTC27930.18113721484508002No Hit
GCACAGGGTCTCTCTCCCAGCTTGCGGTGACTCAATCGCCCTCTGCCTCTGCCTCCCTGGGAGCCTCGGTCAAG27300.17705141300646918No Hit
CTTCATGCGTGACCTGGCAGCTGTAGCTTTTGTGGGACTTCCACTGCTCAGGCGTCAGGCTCAGGTAGCTGCTGG27110.17581918705514213No Hit
CTCTGTTCCCGCCCTCCTCTGAGGAGCTTCAAGCCAACAAGGCCACACTGGTGTGTCTCATAAGTGACTTCTACC27070.17555977106538903No Hit
CAGTAATAGTCACCCTCATCCTCAGACTGGAGGCTGGAGATGGTGAGGTAGCGCTCAGCCCCAGAGCTGGAGC26980.17497608508844464No Hit
GTACGGGAGAGCTCTGGGGAGTCTGCACCATGGCTTGGACCCCACTCCTCTTCCTCACCCTCCTCCTCCACTGCA26920.17458696110381502No Hit
CCCAATTGCCAGTGCCCCAGGTCTGACAGTAATAGTCACCCTCATCCTCA26400.17121455323702514No Hit
CTCATAGAGTGGCCATAAAATCTTTCACCTCCATCTTGTTTTATGGAGGC26330.17076057525495728No Hit
CACCAGTGTGGCCTTGTTGGCTTGAAGCTCCTCAGAGGAGGGCGGGAACAGAGTGACCGAGGGGGCAGCCTTGGG26230.17011203528057459No Hit
CTTATGAGACACACCAGTGTGGCCTTGTTGGCTTGAAGCTCCTCAGAGGA25960.1683609773497414No Hit
CACCTGCACTCTGAGCAGTGGCTACAGCACCTACGTCATCGCTTGGCATC25920.16810156135998833No Hit
GCTCAGGCGTCAGGCTCAGGTAGCTGCTGGCCGCGTACTTGTTGTTGCTTTGTTTGGAGGGTGTGGTGGTCTCCA25670.16648021142403166No Hit
GCTCTGGGGCTGAGCGCTACCTCACCATCTCCAGCCTCCAGTCTGAGGATGAGGGTGACTATTACTGTCAGAC25440.1649885694829515No Hit
CCACTGCACAGGGTCTCTCTCCCAGCTTGCGGTGACTCAATCGCCCTCTGCCTCTGCCTCCCTGGGAGCCTCGGT25360.16446973750344537No Hit
GGCTTGGACCCCACTCCTCTTCCTCACCCTCCTCCTCCACTGCACAGGGT25020.1622647015905443No Hit
TCCTCAGACTGGAGGCTGGAGATGGTGAGGTAGCGCTCAGCCCCAGAGCTGGAGCCTGAGAAGCGATCAGGGACC24690.16012451967508146No Hit
GGGTTTATTGAGTGCAGGGAGAAGGGCTTGATGCCTTGGGGTGGGAGGAGAGACCCCTCCCCTGGGATCCTGCAG24280.15746550578011254No Hit
GTCATAGGTGGTCAGGTCTGTGACCAGGCAGGTCAACTTGGTGGACTTGG24240.15720608979035944No Hit
CCCTTCATGCGTGACCTGGCAGCTGTAGCTTTTGTGGGACTTCCACTGCTCAGGCGTCAGGCTCAGGTAGCTG23800.1543525139030757No Hit
GTATCAACGCAGAGTACGGGAGAGCTCTGGGGAGTCTGCACCATGGCTTGGACCCCACTCCTCTTCCTCACCCTC23130.1500072960747118No Hit
GTAATAGTCACCCTCATCCTCAGACTGGAGGCTGGAGATGGTGAGGTAGC22640.14682945020023672No Hit
GAGAAGGGCTTGATGCCTTGGGGTGGGAGGAGAGACCCCTCCCCTGGGAT22310.1446892682847739No Hit
GGTTTATTGAGTGCAGGGAGAAGGGCTTGATGCCTTGGGGTGGGAGGAGAGACCCCTCCCCTGGGATCCTGCAG22110.14339218833600856No Hit
GTTCCCGCCCTCCTCTGAGGAGCTTCAAGCCAACAAGGCCACACTGGTGT21790.141316860417984No Hit
ACCTAGGACGGTCAGCTTGGTCCCTCCGCCGAACACCCAATTGCCAGTGCCCCAGGTCTGACAGTAATAGTCACC21770.14118715242310748No Hit
GTCTGAGGATGAGGGTGACTATTACTGTCAGACCTGGGGCACTGGCAATTGGGTGTTCGGCGGAGGGACCAAG21730.1409277364333544No Hit
GGCTCAGGTAGCTGCTGGCCGCGTACTTGTTGTTGCTTTGTTTGGAGGGTGTGGTGGTCTCCACTCCCGCCTTGA21580.1399549264717804No Hit
GGCAATTGGGTGTTCGGCGGAGGGACCAAGCTGACCGTCCTAGGTCAGCCCAAGGCTGCCCCCTCGGTCACT21390.13872270052045332No Hit
GGAACAGAGTGACCGAGGGGGCAGCCTTGGGCTGACCTAGGACGGTCAGC21350.13846328453070025No Hit
GAGTACGGGAGAGCTCTGGGGAGTCTGCACCATGGCTTGGACCCCACTCCTCTTCCTCACCCTCCTCCTCCACTG20660.13398835870745984No Hit
CCACCACACCCTCCAAACAAAGCAACAACAAGTACGCGGCCAGCAGCTAC20450.13262642476125622No Hit
TGCACACACAGAGCGGCCAGCCGCCCCGAGCCTGTGGGCAGGCCAGCAGG20390.1322373007766266No Hit
TCCTCACCCTCCTCCTCCACTGCACAGGGTCTCTCTCCCAGCTTGCGGTGACTCAATCGCCCTCTGCCTCTGCCT20200.13100507482529955No Hit
GCTATCTGCCTTCCAGGCCACTGTCACGGCTCCCGGGTAGAAGTCACTTA20140.13061595084066993No Hit
CTCCTCTTCCTCACCCTCCTCCTCCACTGCACAGGGTCTCTCTCCCAGCTTGCGGTGACTCAATCGCCCTCTGC19990.12964314087909595No Hit
CCCCTGGGATCCTGCAGCTCTAGTCTCCCGTGGTGGGGGGTGAGGGTTGAGAACCTATGAACATTCTGTAGGGGC19450.1261410250174295No Hit
GCTCTAGTCTCCCGTGGTGGGGGGTGAGGGTTGAGAACCTATGAACATTC19270.12497365306354072No Hit
CGACAAGGGGGACGGGGTCCCTGATCGCTTCTCAGGCTCCAGCTCTGGGGCTGAGCGCTACCTCACCATCTCCAG19080.12374142711221363No Hit
GAGTGCAGGTGAGCTTGACCGAGGCTCCCAGGGAGGCAGAGGCAGAGGGC19020.12335230312758402No Hit
GCCTTGGGGTGGGAGGAGAGACCCCTCCCCTGGGATCCTGCAGCTCTAGT18950.12289832514551614No Hit
CTACCTCACCATCTCCAGCCTCCAGTCTGAGGATGAGGGTGACTATTACT18900.12257405515832481No Hit
GTGCAGGGCCACCCCCTTGGGCCGGGAGATGGTCTGCTTCAGTGGCGAGGGCAGGTCTGTGTGGGTCACGGTGCA18860.12231463916857176No Hit
ACACTGGTGTGTCTCATAAGTGACTTCTACCCGGGAGCCGTGACAGTGGC18580.12049872724030028No Hit
GACTTCCACTGCTCAGGCGTCAGGCTCAGGTAGCTGCTGGCCGCGTACTT18390.11926650128897319No Hit
GAGTGACCGAGGGGGCAGCCTTGGGCTGACCTAGGACGGTCAGCTTGGTC18220.11816398333252265No Hit
GGTGTGTCTCATAAGTGACTTCTACCCGGGAGCCGTGACAGTGGCCTGGA18100.11738573536326347No Hit
GTCTGCACCATGGCTTGGACCCCACTCCTCTTCCTCACCCTCCTCCTCCA18030.11693175738119559No Hit
GACTATTACTGTCAGACCTGGGGCACTGGCAATTGGGTGTTCGGCGGAGGGACCAAGCTGACCGTCCTAGGTCAG17620.11427274348622662No Hit
GTGTATTACTGTGCGAGACTGCCCCGGTCCAGTGGAAACGCCTATGGCGG17390.11278110154514648No Hit
CTTCTCCACGGTGCTCCCTTCATGCGTGACCTGGCAGCTGTAGCTTTTGT17370.11265139355026996No Hit
TTCCAGGCCACTGTCACGGCTCCCGGGTAGAAGTCACTTATGAGACACACCAGTGTGGCCTTGTTGGCTTGAAG17350.11252168555539342No Hit
CCCTCATCCTCAGACTGGAGGCTGGAGATGGTGAGGTAGCGCTCAGCCCC17100.11090033561943675No Hit
GGGCTGCTATCTGCCTTCCAGGCCACTGTCACGGCTCCCGGGTAGAAGTCACTTATGAGACACACCAGTGTGGC17030.11044635763736887No Hit
CCCTGGGAGCCTCGGTCAAGCTCACCTGCACTCTGAGCAGTGGCTACAGC16920.10973296366554794No Hit
CTGTAGGGGCCACTGTCTTCTCCACGGTGCTCCCTTCATGCGTGACCTGG16560.10739821975777031No Hit
CCTGCACTCTGAGCAGTGGCTACAGCACCTACGTCATCGCTTGGCATCAG16480.1068793877782642No Hit
CTCCAGCTCTGGGGCTGAGCGCTACCTCACCATCTCCAGCCTCCAGTCTG16320.10584172381925191No Hit
TCTCTGGAAGCTGCTGATGCCAAGCGATGACGTAGGTGCTGTAGCCACTG16270.10551745383206058No Hit
GGTAGCTGCTGGCCGCGTACTTGTTGTTGCTTTGTTTGGAGGGTGTGGTGGTCTCCACTCCCGCCTTGACGGGG16230.1052580378423075No Hit
CTCCACTCCCGCCTTGACGGGGCTGCTATCTGCCTTCCAGGCCACTGTCA15990.10370154190378911No Hit
GCTTGGTCCCTCCGCCGAACACCCAATTGCCAGTGCCCCAGGTCTGACAGTAATAGTCACCCTCATCCTCAGA15690.10175592198064108No Hit
GTCTCTCTCCCAGCTTGCGGTGACTCAATCGCCCTCTGCCTCTGCCTCCCTGGGAGCCTCGGTCAAGCTCACCTG15670.10162621398576455No Hit
GTGTGGCCTTGTTGGCTTGAAGCTCCTCAGAGGAGGGCGGGAACAGAGTGACCGAGGGGGCAGCCTTGGGCTGAC15560.10091282001394361No Hit
GGTGTAGGTCTCCCCCGTGTTCCATTCCTCTTCGGACACGGTCAGGATGCTGTGGGCGAAGTACCGGCCTGGGGC15500.100523696029314No Hit
GTCCCTGATCGCTTCTCAGGCTCCAGCTCTGGGGCTGAGCGCTACCTCAC15430.10006971804724614No Hit

[OK]Adapter Content

Adapter graph

[FAIL]Kmer Content

Kmer graph

SequenceCountPValueObs/Exp MaxMax Obs/Exp Position
AGGGGGG5550.088.7521370
CGAATCT5950.046.04720334
TCGTATG6400.045.26313440
TAAGGCG6800.044.64487529
GCGAATC6150.044.50761833
GGCGAAT6850.043.9338432
CTTATAC6900.043.917911
TATACAC7150.043.7882043
TCTCGTA6050.043.75493238
TTATACA7000.043.7542082
CGTATGC6600.043.4360941
AGACTAA7050.042.9876725
ATCTCGT6250.042.85289437
ACGAGAC7100.042.6627922
CACGAGA6950.042.60416421
AGGCGAA7150.042.54796631
GACTAAG7200.042.1112226
GTGGTAT3400.042.060181
GAATCTC6450.041.97956535
GGTATCA11550.041.860761
Produced by FastQC (version 0.11.5)