FastQCFastQC Report
Sat 23 Apr 2022
EGAF00004684436

Summary

[OK]Basic Statistics

MeasureValue
FilenameEGAF00004684436
File typeConventional base calls
EncodingSanger / Illumina 1.9
Total Sequences239307
Sequences flagged as poor quality0
Sequence length21-76
%GC56

[OK]Per base sequence quality

Per base quality graph

[OK]Per tile sequence quality

Per base quality graph

[OK]Per sequence quality scores

Per Sequence quality graph

[FAIL]Per base sequence content

Per base sequence content

[FAIL]Per sequence GC content

Per sequence GC content graph

[OK]Per base N content

N content graph

[WARN]Sequence Length Distribution

Sequence length distribution

[FAIL]Sequence Duplication Levels

Duplication level graph

[WARN]Overrepresented sequences

SequenceCountPercentagePossible Source
GCCCCACTCTGTGTCGGAGTCTCCGGGGAAGACGGTAACCATCTCCTGCACCCGCAGCAGTGGCAGCATTGCCAG10930.45673549039518274No Hit
GCCTTGGGCTGACCTAGGACGGTCAGCTTGGTCCCTCCGCCGAACACCCA9840.4111873033383896No Hit
GCCCAAGGCTGCCCCCTCGGTCACTCTGTTCCCGCCCTCCTCTGAGGAGC9400.39280087920537216No Hit
GTGTCGGAGTCTCCGGGGAAGACGGTAACCATCTCCTGCACCCGCAGCAGTGGCAGCATTGCCAGCAACTCTGTG8500.35519228438783657No Hit
GTGTGGTGGTCTCCACTCCCGCCTTGACGGGGCTGCTATCTGCCTTCCAG8150.3405667197365727No Hit
GACCTAGGACGGTCAGCTTGGTCCCTCCGCCGAACACCCAATTGCTGCTATCATAAGACTGACAGTAGTAGTCAG7930.33137350767006396No Hit
CCTCTGAGGAGCTTCAAGCCAACAAGGCCACACTGGTGTGTCTCATAAGT7140.2983615188857827No Hit
CCTCAGAGGAGGGCGGGAACAGAGTGACCGAGGGGGCAGCCTTGGGCTGACCTAGGACGGTCAGCTTGGTCCCTC6700.2799750947527653No Hit
CCTATGAACATTCTGTAGGGGCCACTGTCTTCTCCACGGTGCTCCCTTCA6440.269110389583255No Hit
TCCTAGGTCAGCCCAAGGCTGCCCCCTCGGTCACTCTGTTCCCGCCCTCCTCTGAGGAGCTTCAAGCCAACAAGG6420.2682746430317542No Hit
GTGCAGGGAGAAGGGCTTGATGCCTTGGGGTGGGAGGAGAGACCCCTCCCCTGGGATCCTGCAGCTCTAGTCTCC6110.2553205714834919No Hit
ACTGAAGACTGAGGACGAGGCTGACTACTACTGTCAGTCTTATGATAGCAGCAATTGGGTGTTCGGCGGAGGGAC5890.24612735941698322No Hit
ACCAAAGACCCTCTGGGGTCCCTGATCGGTTCTCTGGCTCCATCGACAGC5840.24403799303823123No Hit
GTCTTCTCCACGGTGCTCCCTTCATGCGTGACCTGGCAGCTGTAGCTTTT5800.24236649993522966No Hit
GTGGTACCATCAGCGCCCGGGCAGTGCCCCCACCGCTGTGATCTATGAGGATAACCAAAGACCCTCTGGGGTCC5780.24153075338372884No Hit
GACTACTACTGTCAGTCTTATGATAGCAGCAATTGGGTGTTCGGCGGAGGGACCAAGCTGACCGTCCTAGGTCAG5680.2373520206262249No Hit
ATTTTATGCTGACTCAGCCCCACTCTGTGTCGGAGTCTCCGGGGAAGACG5630.23526265424747292No Hit
CTGCACAGGTTCTTGGGCCAATTTTATGCTGACTCAGCCCCACTCTGTGTCGGAGTCTCCGGGGAAGACGGTAAC5620.2348447809717225No Hit
GTAGTAGTCAGCCTCGTCCTCAGTCTTCAGTCGAGAGATGGTGAGGGAGG5530.23108392148996895No Hit
GTGCTCCCTTCATGCGTGACCTGGCAGCTGTAGCTTTTGTGGGACTTCCACTGCTCAGGCGTCAGGCTCAGGTAG5390.22523369562946338No Hit
GTTTGGAGGGTGTGGTGGTCTCCACTCCCGCCTTGACGGGGCTGCTATCTGCCTTCCAGGCCACTGTCACGGCTC5360.22398007580221221No Hit
GGGTTTATTGAGTGCAGGGAGAAGGGCTTGATGCCTTGGGGTGGGAGGAGAGACCCCTCCCCTGGGATCCTGCAG5230.21854772321745708No Hit
CACCAGTGTGGCCTTGTTGGCTTGAAGCTCCTCAGAGGAGGGCGGGAACAGAGTGACCGAGGGGGCAGCCTTGGG5200.21729410339020588No Hit
GTCCTAGGTCAGCCCAAGGCTGCCCCCTCGGTCACTCTGTTCCCGCCCTC5140.2147868637357035No Hit
GTCTTCAGTCGAGAGATGGTGAGGGAGGCAGAGTTGGAGGAGCTGTCGATGGAGCCAGAGAACCGATCAGGGACC5120.21395111718420273No Hit
ATCATAAGACTGACAGTAGTAGTCAGCCTCGTCCTCAGTCTTCAGTCGAG4690.1959825663269357No Hit
CTTCATGCGTGACCTGGCAGCTGTAGCTTTTGTGGGACTTCCACTGCTCAGGCGTCAGGCTCAGGTAGCTGCTGG4690.1959825663269357No Hit
CTTATGAGACACACCAGTGTGGCCTTGTTGGCTTGAAGCTCCTCAGAGGA4670.1951468197754349No Hit
CAGTAGTAGTCAGCCTCGTCCTCAGTCTTCAGTCGAGAGATGGTGAGGGA4650.1943110732239341No Hit
CTCTGTTCCCGCCCTCCTCTGAGGAGCTTCAAGCCAACAAGGCCACACTGGTGTGTCTCATAAGTGACTTCTACC4640.19389319994818371No Hit
GGTTTATTGAGTGCAGGGAGAAGGGCTTGATGCCTTGGGGTGGGAGGAGAGACCCCTCCCCTGGGATCCTGCAG4620.19305745339668293No Hit
GAGAAGGGCTTGATGCCTTGGGGTGGGAGGAGAGACCCCTCCCCTGGGAT4600.19222170684518214No Hit
GTCTTATGATAGCAGCAATTGGGTGTTCGGCGGAGGGACCAAGCTGACCGTCCTAGGTCAGCCCAAGGCTGCCCC4570.19096808701793094No Hit
GTGCAGTGGTACCATCAGCGCCCGGGCAGTGCCCCCACCGCTGTGATCTA4400.1838642413301742No Hit
CCACTACTTCTCACCCTCCTCGCTCACTGCACAGGTTCTTGGGCCAATTT4370.182610621502923No Hit
GCTCAGGCGTCAGGCTCAGGTAGCTGCTGGCCGCGTACTTGTTGTTGCTTTGTTTGGAGGGTGTGGTGGTCTCCA4320.18052125512417105No Hit
CTACTACTGTCAGTCTTATGATAGCAGCAATTGGGTGTTCGGCGGAGGGACCAAGCTGACCGTCCTAGGTCAGCC4200.1755067758151663No Hit
CCCTTCATGCGTGACCTGGCAGCTGTAGCTTTTGTGGGACTTCCACTGCTCAGGCGTCAGGCTCAGGTAGCTG4050.16923867667891035No Hit
CTTATACACATCTCCGAGCCCACGAGACCGAGGCTGATCTCGTATGCCGT4000.16714931030015837No Hit
CCACTATGGCCTGGGCTCCACTACTTCTCACCCTCCTCGCTCACTGCACAGGTTCTTGGGCCAATTTTATGCTGA3980.16631356374865758No Hit
GCCTGGAGCATCTCCACTATGGCCTGGGCTCCACTACTTCTCACCCTCCT3870.16171695771540323No Hit
GGCTCAGGTAGCTGCTGGCCGCGTACTTGTTGTTGCTTTGTTTGGAGGGTGTGGTGGTCTCCACTCCCGCCTTGA3860.16129908443965282No Hit
GGAACAGAGTGACCGAGGGGGCAGCCTTGGGCTGACCTAGGACGGTCAGC3760.15712035168214888No Hit
ACACTGGTGTGTCTCATAAGTGACTTCTACCCGGGAGCCGTGACAGTGGC3750.15670247840639848No Hit
ACTCTGTGCAGTGGTACCATCAGCGCCCGGGCAGTGCCCCCACCGCTGTGATCTATGAGGATAACCAAAGACCCT3650.1525237456488945No Hit
GGCCTGGGCTCCACTACTTCTCACCCTCCTCGCTCACTGCACAGGTTCTTGGGCCAATTTTATGCTGACTCAGCC3600.15043437927014253No Hit
TCTCTCGACTGAAGACTGAGGACGAGGCTGACTACTACTGTCAGTCTTAT3600.15043437927014253No Hit
GCTATCTGCCTTCCAGGCCACTGTCACGGCTCCCGGGTAGAAGTCACTTA3580.14959863271864174No Hit
GCTCTAGTCTCCCGTGGTGGGGGGTGAGGGTTGAGAACCTATGAACATTC3570.14918075944289136No Hit
GGGCTGCTATCTGCCTTCCAGGCCACTGTCACGGCTCCCGGGTAGAAGTCACTTATGAGACACACCAGTGTGGC3530.14750926633988976No Hit
ACCTAGGACGGTCAGCTTGGTCCCTCCGCCGAACACCCAATTGCTGCTATCATAAGACTGACAGTAGTAGTCAGC3520.14709139306413938No Hit
GAGTGACCGAGGGGGCAGCCTTGGGCTGACCTAGGACGGTCAGCTTGGTCCCTCCGCCGAACACCCAATTGCTG3500.1462556465126386No Hit
CCCCTGGGATCCTGCAGCTCTAGTCTCCCGTGGTGGGGGGTGAGGGTTGAGAACCTATGAACATTCTGTAGGGGC3500.1462556465126386No Hit
CTTCTCACCCTCCTCGCTCACTGCACAGGTTCTTGGGCCAATTTTATGCT3490.14583777323688818No Hit
GTTCTCTGGCTCCATCGACAGCTCCTCCAACTCTGCCTCCCTCACCATCTCTCGACTGAAGACTGAGGACGAGG3460.144584153409637No Hit
GCCTTGGGGTGGGAGGAGAGACCCCTCCCCTGGGATCCTGCAGCTCTAGT3440.14374840685813622No Hit
GGTTCTTGGGCCAATTTTATGCTGACTCAGCCCCACTCTGTGTCGGAGTCTCCGGGGAAGACGGTAACCATCTC3410.142494787030885No Hit
GTTCCCGCCCTCCTCTGAGGAGCTTCAAGCCAACAAGGCCACACTGGTGT3400.14207691375513462No Hit
GACTTCCACTGCTCAGGCGTCAGGCTCAGGTAGCTGCTGGCCGCGTACTT3340.13956967410063223No Hit
GTGCCCCCACCGCTGTGATCTATGAGGATAACCAAAGACCCTCTGGGGTC3310.13831605427338106No Hit
GAGTTGGAGGAGCTGTCGATGGAGCCAGAGAACCGATCAGGGACCCCAGA3260.13622668789462908No Hit
GGTGTGTCTCATAAGTGACTTCTACCCGGGAGCCGTGACAGTGGCCTGGA3260.13622668789462908No Hit
GCACAGGTTCTTGGGCCAATTTTATGCTGACTCAGCCCCACTCTGTGTCG3200.13371944824012671No Hit
CTCCACTACTTCTCACCCTCCTCGCTCACTGCACAGGTTCTTGGGCCAATTTTATGCTGACTCAGCCCCACT3200.13371944824012671No Hit
GTGTGGCCTTGTTGGCTTGAAGCTCCTCAGAGGAGGGCGGGAACAGAGTGACCGAGGGGGCAGCCTTGGGCTGAC3140.13121220858562432No Hit
CTGTAGGGGCCACTGTCTTCTCCACGGTGCTCCCTTCATGCGTGACCTGG3140.13121220858562432No Hit
ACTATGGCCTGGGCTCCACTACTTCTCACCCTCCTCGCTCACTGCACAGGTTCTTGGGCCAATTTTATGCTGACT3130.13079433530987392No Hit
CTTCTCCACGGTGCTCCCTTCATGCGTGACCTGGCAGCTGTAGCTTTTGT3110.12995858875837313No Hit
CCACCACACCCTCCAAACAAAGCAACAACAAGTACGCGGCCAGCAGCTAC3080.12870496893112193No Hit
GTCTTTGGTTATCCTCATAGATCACAGCGGTGGGGGCACTGCCCGGGCGCTGATGGTACCACTGCACAGAGTTG3060.12786922237962114No Hit
TTCCAGGCCACTGTCACGGCTCCCGGGTAGAAGTCACTTATGAGACACACCAGTGTGGCCTTGTTGGCTTGAAG2940.1228547430706164No Hit
GGTAGCTGCTGGCCGCGTACTTGTTGTTGCTTTGTTTGGAGGGTGTGGTGGTCTCCACTCCCGCCTTGACGGGG2940.1228547430706164No Hit
GTTGAGAACCTATGAACATTCTGTAGGGGCCACTGTCTTCTCCACGGTGCTCCCTTCATGCGTGACCTGGCAG2890.12076537669186442No Hit
TCTCCACTATGGCCTGGGCTCCACTACTTCTCACCCTCCTCGCTCACTGCACAGGTTCTTGGGCCAATTTTATG2830.11825813703736204No Hit
GCTTGGTCCCTCCGCCGAACACCCAATTGCTGCTATCATAAGACTGACAG2830.11825813703736204No Hit
CTGTCACGGCTCCCGGGTAGAAGTCACTTATGAGACACACCAGTGTGGCC2710.1132436577283573No Hit
CGGTCACTCTGTTCCCGCCCTCCTCTGAGGAGCTTCAAGCCAACAAGGCC2680.11199003790110611No Hit
GTAATACATGGCGGTGTCCGAGGCCTTCAGGCTGCTCCACTGCAGGTAGG2620.10948279824660374No Hit
CTCCTGCACCCGCAGCAGTGGCAGCATTGCCAGCAACTCTGTGCAGTGGTACCATCAGCGCCCGGGCAGTGCCCC2610.10906492497085334No Hit
TGGTACCACTGCACAGAGTTGCTGGCAATGCTGCCACTGCTGCGGGTGCA2590.10822917841935255No Hit
GTATCAACGCAGAGTACGGGGTAGCTCAGGAAGCAGAGCCTGGAGCATCT2560.10697555859210137No Hit
CCAAAGACCCTCTGGGGTCCCTGATCGGTTCTCTGGCTCCATCGACAGCTCCTCCAACTCTGCCTCCCTCACCAT2520.10530406548909979No Hit
ACTTCTCACCCTCCTCGCTCACTGCACAGGTTCTTGGGCCAATTTTATGC2520.10530406548909979No Hit
CCCCAGAGGGTCTTTGGTTATCCTCATAGATCACAGCGGTGGGGGCACTGCCCGGGCGCTGATGGTACCACTGCA2510.10488619221334938No Hit
CCTCCAACTCTGCCTCCCTCACCATCTCTCGACTGAAGACTGAGGACGAG2490.1040504456618486No Hit
GATCTATGAGGATAACCAAAGACCCTCTGGGGTCCCTGATCGGTTCTCTGGCTCCATCGACAGCTCCTCCAACT2460.1027968258345974No Hit
GGGTTGAGAACCTATGAACATTCTGTAGGGGCCACTGTCTTCTCCACGGTGCTCCCTTCATGCGTGACCTGGCAG2460.1027968258345974No Hit
GGGTAGAAGTCACTTATGAGACACACCAGTGTGGCCTTGTTGGCTTGAAG2430.10154320600734622No Hit
GTCGAGAGATGGTGAGGGAGGCAGAGTTGGAGGAGCTGTCGATGGAGCCA2430.10154320600734622No Hit
ATTCTGTAGGGGCCACTGTCTTCTCCACGGTGCTCCCTTCATGCGTGACC2430.10154320600734622No Hit
ATGTATTACTGTGCGAGACGACGTGGATACAGCTATGGTTACGGCGAGTACTTTGACTACTGGGGCCAGGGAACC2400.10028958618009502No Hit

[OK]Adapter Content

Adapter graph

[FAIL]Kmer Content

Kmer graph

SequenceCountPValueObs/Exp MaxMax Obs/Exp Position
AGGGGGG900.088.44327570
TGTCGTA150.002360772668.9444138
TCACCTA150.002430540768.4400123
GTTAGTC150.002432556668.425719
TTAGTCC150.002432556668.4257110
CGTTTTT150.002434573468.41141514
AAAGGGG700.059.5224968
AACAGGT200.006580215453.2212360
TCGTATG800.051.76280640
CTTATAC800.051.3300061
TCTCCGA800.051.3192811
CCCACGA750.050.17885219
GTATGCC900.049.9826342
CGTATGC850.048.7590541
ACGAGAC850.048.31059322
ATCTCCG850.048.300510
ACGGGTC507.384551E-847.8985
AAAAGGG850.047.04820367
CACGAGA800.047.0426721
TTATACA900.045.6076052
Produced by FastQC (version 0.11.5)