FastQCFastQC Report
Sat 23 Apr 2022
EGAF00004684437

Summary

[OK]Basic Statistics

MeasureValue
FilenameEGAF00004684437
File typeConventional base calls
EncodingSanger / Illumina 1.9
Total Sequences239307
Sequences flagged as poor quality0
Sequence length20-76
%GC56

[OK]Per base sequence quality

Per base quality graph

[OK]Per tile sequence quality

Per base quality graph

[OK]Per sequence quality scores

Per Sequence quality graph

[FAIL]Per base sequence content

Per base sequence content

[FAIL]Per sequence GC content

Per sequence GC content graph

[OK]Per base N content

N content graph

[WARN]Sequence Length Distribution

Sequence length distribution

[FAIL]Sequence Duplication Levels

Duplication level graph

[WARN]Overrepresented sequences

SequenceCountPercentagePossible Source
GCCTTGGGCTGACCTAGGACGGTCAGCTTGGTCCCTCCGCCGAACACCCA9480.3961438654113753No Hit
GCCCCACTCTGTGTCGGAGTCTCCGGGGAAGACGGTAACCATCTCCTGCACCCGCAGCAGTGGCAGCATTGCCAG9310.3890400197236186No Hit
GTGTCGGAGTCTCCGGGGAAGACGGTAACCATCTCCTGCACCCGCAGCAGTGGCAGCATTGCCAGCAACTCTGTG9210.38486128696611466No Hit
GCCCAAGGCTGCCCCCTCGGTCACTCTGTTCCCGCCCTCCTCTGAGGAGC8650.3614603835240925No Hit
GTGTGGTGGTCTCCACTCCCGCCTTGACGGGGCTGCTATCTGCCTTCCAG8270.34558119904557744No Hit
GGGGGGGGGGGGGGGGGGGGGGGGGGGGGGGGGGGGGGGGGGGGGGGGGG8270.34558119904557744No Hit
GACCTAGGACGGTCAGCTTGGTCCCTCCGCCGAACACCCAATTGCTGCTATCATAAGACTGACAGTAGTAGTCAG7680.32092667577630407No Hit
CCTCTGAGGAGCTTCAAGCCAACAAGGCCACACTGGTGTGTCTCATAAGT6880.28749681371627245No Hit
CCTCAGAGGAGGGCGGGAACAGAGTGACCGAGGGGGCAGCCTTGGGCTGACCTAGGACGGTCAGCTTGGTCCCTC6660.2783036016497637No Hit
CTGCACAGGTTCTTGGGCCAATTTTATGCTGACTCAGCCCCACTCTGTGTCGGAGTCTCCGGGGAAGACGGTAAC6100.25490269820774153No Hit
GTAGTAGTCAGCCTCGTCCTCAGTCTTCAGTCGAGAGATGGTGAGGGAGG6010.25114183872598794No Hit
CCTATGAACATTCTGTAGGGGCCACTGTCTTCTCCACGGTGCTCCCTTCA5940.2482167257957352No Hit
TCCTAGGTCAGCCCAAGGCTGCCCCCTCGGTCACTCTGTTCCCGCCCTCCTCTGAGGAGCTTCAAGCCAACAAGG5910.24696310596848398No Hit
GTGGTACCATCAGCGCCCGGGCAGTGCCCCCACCGCTGTGATCTATGAGGATAACCAAAGACCCTCTGGGGTCC5820.24320224648673042No Hit
GTCTTCTCCACGGTGCTCCCTTCATGCGTGACCTGGCAGCTGTAGCTTTT5740.23985926028072727No Hit
GACTACTACTGTCAGTCTTATGATAGCAGCAATTGGGTGTTCGGCGGAGGGACCAAGCTGACCGTCCTAGGTCAG5670.2369341473504745No Hit
GGGTTTATTGAGTGCAGGGAGAAGGGCTTGATGCCTTGGGGTGGGAGGAGAGACCCCTCCCCTGGGATCCTGCAG5610.2344269076959721No Hit
ATTTTATGCTGACTCAGCCCCACTCTGTGTCGGAGTCTCCGGGGAAGACG5520.23066604821421854No Hit
GTGCTCCCTTCATGCGTGACCTGGCAGCTGTAGCTTTTGTGGGACTTCCACTGCTCAGGCGTCAGGCTCAGGTAG5440.2273230620082154No Hit
ACTGAAGACTGAGGACGAGGCTGACTACTACTGTCAGTCTTATGATAGCAGCAATTGGGTGTTCGGCGGAGGGAC5380.22481582235371303No Hit
ACCAAAGACCCTCTGGGGTCCCTGATCGGTTCTCTGGCTCCATCGACAGC5310.22189070942346023No Hit
ATCATAAGACTGACAGTAGTAGTCAGCCTCGTCCTCAGTCTTCAGTCGAG5180.2164583568387051No Hit
GTTTGGAGGGTGTGGTGGTCTCCACTCCCGCCTTGACGGGGCTGCTATCTGCCTTCCAGGCCACTGTCACGGCTC5160.2156226102872043No Hit
GTCTTCAGTCGAGAGATGGTGAGGGAGGCAGAGTTGGAGGAGCTGTCGATGGAGCCAGAGAACCGATCAGGGACC5100.21311537063270192No Hit
GTGCAGGGAGAAGGGCTTGATGCCTTGGGGTGGGAGGAGAGACCCCTCCCCTGGGATCCTGCAGCTCTAGTCTCC5070.21186175080545075No Hit
GTCTTATGATAGCAGCAATTGGGTGTTCGGCGGAGGGACCAAGCTGACCGTCCTAGGTCAGCCCAAGGCTGCCCC4800.20057917236019004No Hit
CAGTAGTAGTCAGCCTCGTCCTCAGTCTTCAGTCGAGAGATGGTGAGGGA4630.1934753266724333No Hit
CTCTGTTCCCGCCCTCCTCTGAGGAGCTTCAAGCCAACAAGGCCACACTGGTGTGTCTCATAAGTGACTTCTACC4610.19263958012093252No Hit
CTTATGAGACACACCAGTGTGGCCTTGTTGGCTTGAAGCTCCTCAGAGGA4580.19138596029368132No Hit
GCTCAGGCGTCAGGCTCAGGTAGCTGCTGGCCGCGTACTTGTTGTTGCTTTGTTTGGAGGGTGTGGTGGTCTCCA4550.19013234046643016No Hit
GTCCTAGGTCAGCCCAAGGCTGCCCCCTCGGTCACTCTGTTCCCGCCCTC4470.186789354260427No Hit
GTGCAGTGGTACCATCAGCGCCCGGGCAGTGCCCCCACCGCTGTGATCTA4430.1851178611574254No Hit
CTTCATGCGTGACCTGGCAGCTGTAGCTTTTGTGGGACTTCCACTGCTCAGGCGTCAGGCTCAGGTAGCTGCTGG4310.18010338184842065No Hit
GGTTTATTGAGTGCAGGGAGAAGGGCTTGATGCCTTGGGGTGGGAGGAGAGACCCCTCCCCTGGGATCCTGCAG4120.17216378960916312No Hit
CACCAGTGTGGCCTTGTTGGCTTGAAGCTCCTCAGAGGAGGGCGGGAACAGAGTGACCGAGGGGGCAGCCTTGGG4090.17091016978191192No Hit
GGCTCAGGTAGCTGCTGGCCGCGTACTTGTTGTTGCTTTGTTTGGAGGGTGTGGTGGTCTCCACTCCCGCCTTGA4060.16965654995466076No Hit
GTTCTCTGGCTCCATCGACAGCTCCTCCAACTCTGCCTCCCTCACCATCTCTCGACTGAAGACTGAGGACGAGG4060.16965654995466076No Hit
GAGAAGGGCTTGATGCCTTGGGGTGGGAGGAGAGACCCCTCCCCTGGGAT4030.16840293012740956No Hit
CCCTTCATGCGTGACCTGGCAGCTGTAGCTTTTGTGGGACTTCCACTGCTCAGGCGTCAGGCTCAGGTAGCTG4020.16798505685165918No Hit
CTACTACTGTCAGTCTTATGATAGCAGCAATTGGGTGTTCGGCGGAGGGACCAAGCTGACCGTCCTAGGTCAGCC3840.16046333788815204No Hit
GAGTTGGAGGAGCTGTCGATGGAGCCAGAGAACCGATCAGGGACCCCAGA3760.15712035168214888No Hit
GGAACAGAGTGACCGAGGGGGCAGCCTTGGGCTGACCTAGGACGGTCAGC3670.1533594922003953No Hit
GGTTCTTGGGCCAATTTTATGCTGACTCAGCCCCACTCTGTGTCGGAGTCTCCGGGGAAGACGGTAACCATCTC3650.1525237456488945No Hit
GAGTGACCGAGGGGGCAGCCTTGGGCTGACCTAGGACGGTCAGCTTGGTCCCTCCGCCGAACACCCAATTGCTG3560.14876288616714095No Hit
GTCTTTGGTTATCCTCATAGATCACAGCGGTGGGGGCACTGCCCGGGCGCTGATGGTACCACTGCACAGAGTTG3560.14876288616714095No Hit
ACCTAGGACGGTCAGCTTGGTCCCTCCGCCGAACACCCAATTGCTGCTATCATAAGACTGACAGTAGTAGTCAGC3510.14667351978838897No Hit
GCTCTAGTCTCCCGTGGTGGGGGGTGAGGGTTGAGAACCTATGAACATTC3460.144584153409637No Hit
ACTCTGTGCAGTGGTACCATCAGCGCCCGGGCAGTGCCCCCACCGCTGTGATCTATGAGGATAACCAAAGACCCT3450.1441662801338866No Hit
ACACTGGTGTGTCTCATAAGTGACTTCTACCCGGGAGCCGTGACAGTGGC3410.142494787030885No Hit
GAGTTGCTGGCAATGCTGCCACTGCTGCGGGTGCAGGAGATGGTTACCGT3370.14082329392788343No Hit
GACTTCCACTGCTCAGGCGTCAGGCTCAGGTAGCTGCTGGCCGCGTACTT3360.14040542065213302No Hit
GTTCCCGCCCTCCTCTGAGGAGCTTCAAGCCAACAAGGCCACACTGGTGT3340.13956967410063223No Hit
GCTATCTGCCTTCCAGGCCACTGTCACGGCTCCCGGGTAGAAGTCACTTA3290.13748030772188027No Hit
GGTGTGTCTCATAAGTGACTTCTACCCGGGAGCCGTGACAGTGGCCTGGA3280.13706243444612987No Hit
TCTCTCGACTGAAGACTGAGGACGAGGCTGACTACTACTGTCAGTCTTAT3220.1345551947916275No Hit
GCCTGGAGCATCTCCACTATGGCCTGGGCTCCACTACTTCTCACCCTCCT3210.1341373215158771No Hit
GGGCTGCTATCTGCCTTCCAGGCCACTGTCACGGCTCCCGGGTAGAAGTCACTTATGAGACACACCAGTGTGGC3140.13121220858562432No Hit
GTGTGGCCTTGTTGGCTTGAAGCTCCTCAGAGGAGGGCGGGAACAGAGTGACCGAGGGGGCAGCCTTGGGCTGAC3040.12703347582812036No Hit
CTTCTCCACGGTGCTCCCTTCATGCGTGACCTGGCAGCTGTAGCTTTTGT3010.1257798560008692No Hit
CCACCACACCCTCCAAACAAAGCAACAACAAGTACGCGGCCAGCAGCTAC2930.122436869794866No Hit
TTCCAGGCCACTGTCACGGCTCCCGGGTAGAAGTCACTTATGAGACACACCAGTGTGGCCTTGTTGGCTTGAAG2930.122436869794866No Hit
CCACTATGGCCTGGGCTCCACTACTTCTCACCCTCCTCGCTCACTGCACAGGTTCTTGGGCCAATTTTATGCTGA2920.12201899651911562No Hit
GCTTGGTCCCTCCGCCGAACACCCAATTGCTGCTATCATAAGACTGACAG2920.12201899651911562No Hit
GCCTTGGGGTGGGAGGAGAGACCCCTCCCCTGGGATCCTGCAGCTCTAGT2860.11951175686461324No Hit
CCCCTGGGATCCTGCAGCTCTAGTCTCCCGTGGTGGGGGGTGAGGGTTGAGAACCTATGAACATTCTGTAGGGGC2860.11951175686461324No Hit
GCACAGGTTCTTGGGCCAATTTTATGCTGACTCAGCCCCACTCTGTGTCG2850.11909388358886284No Hit
GGTAGCTGCTGGCCGCGTACTTGTTGTTGCTTTGTTTGGAGGGTGTGGTGGTCTCCACTCCCGCCTTGACGGGG2800.11700451721011085No Hit
GTTGAGAACCTATGAACATTCTGTAGGGGCCACTGTCTTCTCCACGGTGCTCCCTTCATGCGTGACCTGGCAG2730.11407940427985809No Hit
AGACACACCAGTGTGGCCTTGTTGGCTTGAAGCTCCTCAGAGGAGGGCGGGAACAGAGTGACCGAGGGGGCAGC2720.1136615310041077No Hit
GGGCGGGAACAGAGTGACCGAGGGGGCAGCCTTGGGCTGACCTAGGACGGTCAGCTTGGTCCCTCCGCCGAACAC2690.11240791117685651No Hit
TGGTACCACTGCACAGAGTTGCTGGCAATGCTGCCACTGCTGCGGGTGCA2630.10990067152235412No Hit
GTATCAACGCAGAGTACGGGGTAGCTCAGGAAGCAGAGCCTGGAGCATCT2620.10948279824660374No Hit
CCTCCAACTCTGCCTCCCTCACCATCTCTCGACTGAAGACTGAGGACGAG2580.10781130514360215No Hit
CCACTACTTCTCACCCTCCTCGCTCACTGCACAGGTTCTTGGGCCAATTT2580.10781130514360215No Hit
GGTACCATCAGCGCCCGGGCAGTGCCCCCACCGCTGTGATCTATGAGGAT2510.10488619221334938No Hit
GGGGTGGGAGGAGAGACCCCTCCCCTGGGATCCTGCAGCTCTAGTCTCCCGTGGTGGGGGGTGAGGGTTGAGAAC2490.1040504456618486No Hit
GAGTGGGGCTGAGTCAGCATAAAATTGGCCCAAGAACCTGTGCAGTGAGCGAGGAGGGTGAGAAGTAGTGGAGCC2460.1027968258345974No Hit
ACCTGAGCCTGACGCCTGAGCAGTGGAAGTCCCACAAAAGCTACAGCTGC2440.1019610792830966No Hit
CTCCTGCACCCGCAGCAGTGGCAGCATTGCCAGCAACTCTGTGCAGTGGTACCATCAGCGCCCGGGCAGTGCCCC2440.1019610792830966No Hit
GTGCCCCCACCGCTGTGATCTATGAGGATAACCAAAGACCCTCTGGGGTC2440.1019610792830966No Hit
CTGTAGGGGCCACTGTCTTCTCCACGGTGCTCCCTTCATGCGTGACCTGG2440.1019610792830966No Hit
CTCCACTCCCGCCTTGACGGGGCTGCTATCTGCCTTCCAGGCCACTGTCA2430.10154320600734622No Hit
CCCCAGAGGGTCTTTGGTTATCCTCATAGATCACAGCGGTGGGGGCACTGCCCGGGCGCTGATGGTACCACTGCA2420.10112533273159581No Hit

[OK]Adapter Content

Adapter graph

[WARN]Kmer Content

Kmer graph

SequenceCountPValueObs/Exp MaxMax Obs/Exp Position
TACGAGC150.002074634771.2267962
CGGTCTC200.007637385351.2538159
GCCGAGT305.569621E-447.1659858
TCTCGCG606.366519E-445.88504870
GTGGTAT603.104742E-739.8974341
GACAGAC350.001405149639.0832021
GTATCAA5650.036.316251
GGTATCA3550.035.642781
AATGGTA1001.8788327E-434.4137970
ACAGGAC501.9555431E-434.1978071
TCTCGCT400.002717640334.16921211
ACAGGGG652.4573028E-531.5672041
TATCAAC6750.030.359942
ATCAACG7200.028.4624443
TCAACGC7300.028.0725464
GACAGGT500.008061605527.3582441
ACAGGCC500.008061605527.3582441
GGGGACG500.00811111427.3239485
AACGCAG7550.027.1486646
CAACGCA7550.027.1429925
Produced by FastQC (version 0.11.5)