FastQCFastQC Report
Sat 23 Apr 2022
EGAF00004684438

Summary

[OK]Basic Statistics

MeasureValue
FilenameEGAF00004684438
File typeConventional base calls
EncodingSanger / Illumina 1.9
Total Sequences1328556
Sequences flagged as poor quality0
Sequence length20-76
%GC55

[OK]Per base sequence quality

Per base quality graph

[OK]Per tile sequence quality

Per base quality graph

[OK]Per sequence quality scores

Per Sequence quality graph

[FAIL]Per base sequence content

Per base sequence content

[FAIL]Per sequence GC content

Per sequence GC content graph

[OK]Per base N content

N content graph

[WARN]Sequence Length Distribution

Sequence length distribution

[FAIL]Sequence Duplication Levels

Duplication level graph

[WARN]Overrepresented sequences

SequenceCountPercentagePossible Source
CTCTAACACTCTCCCCTGTTGAAGCTCTTTGTGACGGGCGAGCTCAGGCC55360.41669301105862305No Hit
CTCCTGGGAGTTACCCGATTGGAGGGCGTTATCCACCTTCCACTGTACTTTGGCCTCTCTGGGATAGAAGTTATT52680.39652073378916664No Hit
CTCCTAAGCTGCTCATTTACTGGGCGTCTTCCCGGGAATCCGGGGTCCCT47470.3573052246198128No Hit
GTTTTATACAACTCCAACAATAAGAACTACTTAGCTTGGTACCAGCAGAA47270.35579983079373395No Hit
CTTTTGGCCAGGGGACCAAGCTGGAGCTCAAACGAACTGTGGCTGCACCA44570.3354770141416696No Hit
ATATTATAGTAATCCACCACACACTTTTGGCCAGGGGACCAAGCTGGAGCTCAAACGAACTGTGGCTGCACCAT44080.33178879926777644No Hit
GCTTAGGAGGCTGTCCTGGTTTCTGCTGGTACCAAGCTAAGTAGTTCTTATTGTTGGAGTTGTATAAAACACT42580.32049834557218515No Hit
CTATAATATTGCTGACAGTAATAAACTGCCACATCTTCAGCCTGCAGGCTGCTGATGGTGAGAGTGAAATCTGTC38350.28865926615061765No Hit
GTCATAGGTGGTCAGGTCTGTGACCAGGCAGGTCAACTTGGTGGACTTGG35280.26555147092030745No Hit
GGCTGGAACTGAGGAGCAGGTGGGGGCACTTCTCCCTCTAACACTCTCCC32950.24801363284648897No Hit
CTCCCAGGAGAGTGTCACAGAGCAGGACAGCAAGGACAGCACCTACAGCC32490.24455122704650764No Hit
CAACAGAGGCAGTTCCAGATTTCAACTGCTCATCAGATGGCGGGAAGATG29520.22219612872923683No Hit
GTACAGTGGAAGGTGGATAACGCCCTCCAATCGGGTAACTCCCAGGAGAG28840.2170777897205688No Hit
GTCCTGCTCTGTGACACTCTCCTGGGAGTTACCCGATTGGAGGGCGTTATCCACCTTCCACTGTACTTTGGCCT28760.21647563219013727No Hit
GGATGGGAGGGGGTCAGGCTGGAACTGAGGAGCAGGTGGGGGCACTTCTCCCTCTAACACTCTCCCCTGTTGAAG28620.2154218565118821No Hit
GTGCAGGGCCACCCCCTTGGGCCGGGAGATGGTCTGCTTCAGTGGCGAGGGCAGGTCTGTGTGGGTCACGGTGCA27880.2098518993553904No Hit
CAATAATACATGGCCGTGTCCTCGGCTCTCAGGCTGTTCATTTGAAGATA27870.20977662966408642No Hit
GGCGTAGAGTTTGTGTTTCTCGTAGTCTGCTTTGCTCAGCGTCAGGGTGC27660.20819596614670366No Hit
CAATAGGGGTAGGTCCCCTGTGGAAAAAGGGTCAGAGGCCAAAGGATGGGAGGGGGTCAGGCTGGAACTGAGGAG27580.20759380861627208No Hit
GCGTCAGGGTGCTGCTGAGGCTGTAGGTGCTGTCCTTGCTGTCCTGCTCT27440.20654003293801693No Hit
GGCCAAAAGTGTGTGGTGGATTACTATAATATTGCTGACAGTAATAAACTGCCACATCTTCAGCCTGCAGGCTG27120.20413140281629075No Hit
GTAATAAACTGCCACATCTTCAGCCTGCAGGCTGCTGATGGTGAGAGTGAAATCTGTCCCAGACCCGCTGGCA26600.20021737886848578No Hit
GTCTTCATCTTCCCGCCATCTGATGAGCAGTTGAAATCTGGAACTGCCTCTGTTGTGTGCCTGCTGAATAACTT24570.18493763153378553No Hit
GTTTATTACTGTCAGCAATATTATAGTAATCCACCACACACTTTTGGCCA24400.18365804678161854No Hit
GTATAAAACACTCTGGCTGGACTTGCAGTTGATGGTGGCCCTCTCGCCCAGAGACACAGCCAGGGAGTCTGGAGA23920.18004510159902934No Hit
GTATTATTGTGCGAGACACTCTGGAAGCAGCTGGGTTGACTACTGGGGCC23490.17680850487295982No Hit
TGCACACACAGAGCGGCCAGCCGCCCCGAGCCTGTGGGCAGGCCAGCAGG23200.17462568382514548No Hit
GTGTTGCAGACCCAGGTCTTCATTTCTCTGTTGCTCTGGATCTCTGGTGC23100.17387298691210606No Hit
CCACTGTACTTTGGCCTCTCTGGGATAGAAGTTATTCAGCAGGCACACAACAGAGGCAGTTCCAGATTTCAACTG23090.17379771722080214No Hit
CTCTGGGATAGAAGTTATTCAGCAGGCACACAACAGAGGCAGTTCCAGAT22880.17221705370341933No Hit
CTTATACACATCTCCGAGCCCACGAGACTAGCGCTCATCTCGTATGCCGT22800.1716148961729878No Hit
ACCTATGACAGCGTGACCATCTCCTGGACCCGCCAGAATGGCGAAGCTGT21600.16258253321651478No Hit
GTGGCTGCACCATCTGTCTTCATCTTCCCGCCATCTGATGAGCAGTTGAAATCTGGAACTGCCTCTGTTGTGTGC21590.16250726352521083No Hit
GTCTCCAGACTCCCTGGCTGTGTCTCTGGGCGAGAGGGCCACCATCAACTGCAAGTCCAGCCAGAGTGTTTTATA21390.161001869699132No Hit
GGTGTAGGTCTCCCCCGTGTTCCATTCCTCTTCGGACACGGTCAGGATGCTGTGGGCGAAGTACCGGCCTGGGGC21330.16055025155130834No Hit
CTCTACGCCTGCGAAGTCACCCATCAGGGCCTGAGCTCGCCCGTCACAAA20590.15498029439481661No Hit
GGCTGTAGGTGCTGTCCTTGCTGTCCTGCTCTGTGACACTCTCCTGGGAGTTACCCGATTGGAGGGCGTTATCCA20240.15234585519917865No Hit
GGCCAAAGGATGGGAGGGGGTCAGGCTGGAACTGAGGAGCAGGTGGGGGCACTTCTCCCTCTAACACTCTCCC20160.15174369766874712No Hit
CTCCCTGGCTGTGTCTCTGGGCGAGAGGGCCACCATCAACTGCAAGTCCAGCCAGAGTGTTTTATACAACTCCAA19950.15016303415136434No Hit
GATGAAGACAGATGGTGCAGCCACAGTTCGTTTGAGCTCCAGCTTGGTCC19820.14918452816441308No Hit
GTCCCTGACCGATTCAGTGCCAGCGGGTCTGGGACAGATTTCACTCTCAC19360.14572212236443174No Hit
ATCCCAGAGAGGCCAAAGTACAGTGGAAGGTGGATAACGCCCTCCAATCG19060.14346403162531352No Hit
GAGCAGGACAGCAAGGACAGCACCTACAGCCTCAGCAGCACCCTGACGCT18910.14233498625575436No Hit
AACCAGGACAGCCTCCTAAGCTGCTCATTTACTGGGCGTCTTCCCGGGAATCCGGGGTCCCTGACCGATTCAGTG18760.14120594088619523No Hit
GCCCTGCACAGGCCCGATGTCTACTTGCTGCCACCAGCCCGGGAGCAGCT18620.14015216520794005No Hit
GTGCAGCCACAGTTCGTTTGAGCTCCAGCTTGGTCCCCTGGCCAAAAGTGTGTGGTGGATTACTATAATATTG18340.13804461385142966No Hit
GAGTGAAATCTGTCCCAGACCCGCTGGCACTGAATCGGTCAGGGACCCCG18070.13601233218622324No Hit
ATTCAGTGCCAGCGGGTCTGGGACAGATTTCACTCTCACCATCAGCAGCC17930.13495855650796804No Hit
GATTTCACTCTCACCATCAGCAGCCTGCAGGCTGAAGATGTGGCAGTTTATTACTGTCAGCAATATTATAGTAAT17930.13495855650796804No Hit
GTTGTGTGCCTGCTGAATAACTTCTATCCCAGAGAGGCCAAAGTACAGTG17920.13488328681666412No Hit
GTATGTGACCAGCGCCCCAATGCCTGAGCCCCAGGCCCCAGGCCGGTACTTCGCCCACAGCATCCTGACCGTGTC17920.13488328681666412No Hit
GTCCCAGACCCGCTGGCACTGAATCGGTCAGGGACCCCGGATTCCCGGGA17880.13458220805144833No Hit
GGCCCTGATGGGTGACTTCGCAGGCGTAGAGTTTGTGTTTCTCGTAGTCTGCTTTGCTCAGCGTCAGGGTGCTG17860.13443166866884046No Hit
CCCTGGCTGTGTCTCTGGGCGAGAGGGCCACCATCAACTGCAAGTCCAGC17670.13300154453406557No Hit
CTCCTGTGCAGCCTCTGGGTTCGCCGTCAGTGACATGTTCATGAGCTGGGTCCGCCAGGCTCCAGAGAAGGGG17660.13292627484276162No Hit
GCTTTGCTCAGCGTCAGGGTGCTGCTGAGGCTGTAGGTGCTGTCCTTGCT17430.13119507194277097No Hit
GCACTGAACACAGAGGACTCACCATGGAGTTTTGGCTGAGCTGGGTTTTC17380.13081872348625123No Hit
GCTCATTTACTGGGCGTCTTCCCGGGAATCCGGGGTCCCTGACCGATTCAGTGCCAGCGGGTCTGGGACAGATTT17170.12923805996886847No Hit
GTCAGGGACCCCGGATTCCCGGGAAGACGCCCAGTAAATGAGCAGCTTAGGAGGCTGTCCTGGTTTCTGCTGGTA16600.12494768756454377No Hit
TCTTCAGCCTGCAGGCTGCTGATGGTGAGAGTGAAATCTGTCCCAGACCCGCTGGCACTGAATCGGTCAGGGACC16580.12479714818193588No Hit
GTAAATGAGCAGCTTAGGAGGCTGTCCTGGTTTCTGCTGGTACCAAGCTA16210.12201216960369002No Hit
GTCCTGAGAGGGGGCAAGTACGCAGCCACCTCACAGGTGCTGCTGCCTTC16200.12193689991238608No Hit
CTACGTAGACTCCGTGAAGGGCCGATTCACCATCTCCAGAGACAGTTCCAAGAACACTCTCTATCTTCAAATGAA16020.12058204546891511No Hit
GACCGATTCAGTGCCAGCGGGTCTGGGACAGATTTCACTCTCACCATCAG16000.12043150608630725No Hit
GTCCTTGCTGTCCTGCTCTGTGACACTCTCCTGGGAGTTACCCGATTGGAGGGCGTTATCCACCTTCCACTGTA15830.11915192133414024No Hit
GTCCTGGTTTCTGCTGGTACCAAGCTAAGTAGTTCTTATTGTTGGAGTTG15790.11885084256892445No Hit
CAGTAATAAACTGCCACATCTTCAGCCTGCAGGCTGCTGATGGTGAGAGTGAAATCTGTCCCAGACCCGCTGGCA15310.11523789738633525No Hit
CTCCATGTGTGTCCCCGATCAAGACACAGCCATCCGGGTCTTCGCCATCCCCCCATCCTTTGCCAGCATCTTCCT14930.11237764911678544No Hit
GGCAGGGGCAGCAAGATGGTGTTGCAGACCCAGGTCTTCATTTCTCTGTT14840.11170022189504997No Hit
CTCCTGGACCCGCCAGAATGGCGAAGCTGTGAAAACCCACACCAACATCTCCGAGAGCCACCCCAATGCCACTTT14840.11170022189504997No Hit
GTTGAAGCTCTTTGTGACGGGCGAGCTCAGGCCCTGATGGGTGACTTCGC14800.1113991431298342No Hit
GTACTTTGGCCTCTCTGGGATAGAAGTTATTCAGCAGGCACACAACAGAGGCAGTTCCAGATTTCAACTGCTCAT14770.11117333405592238No Hit
CCCCTGTTGAAGCTCTTTGTGACGGGCGAGCTCAGGCCCTGATGGGTGACTTCGCAGGCGTAGAGTTTGTGTTT14580.10974320992114747No Hit
GTCTCTGGGCGAGAGGGCCACCATCAACTGCAAGTCCAGCCAGAGTGTTTTATACAACTCCAACAATAAGAACTA14440.10868943424289228No Hit
GTGGAAAAAGGGTCAGAGGCCAAAGGATGGGAGGGGGTCAGGCTGGAACTGAGGAGCAGGTGGGGGCACTTCTCC14240.10718404041681344No Hit
CCTATGACAGCGTGACCATCTCCTGGACCCGCCAGAATGGCGAAGCTGTG14220.10703350103420554No Hit
GTACAGGGTGGGTTTACCGGTGGACTTGTCCACGGTCCTCTCGGTGACCCTGTTGGGCAGGGCCTCATGGGCCAC14030.10560337689943067No Hit
TCTCAGGACTGATGGGAAGCCCCGGGTGCTGCTGATGTCAGAGTTGTTCT14010.10545283751682277No Hit
GAGCAAAGCAGACTACGAGAAACACAAACTCTACGCCTGCGAAGTCACCC13950.10500121936899913No Hit
CTTCTATCCCAGAGAGGCCAAAGTACAGTGGAAGGTGGATAACGCCCTCCAATCGGGTAACTCCCAGGAGAGTGT13940.1049259496776952No Hit
GGGTAGGTCCCCTGTGGAAAAAGGGTCAGAGGCCAAAGGATGGGAGGGGG13940.1049259496776952No Hit
GGATCTCTGGTGCCTACGGGGCCATCGTGATGACCCAGTCTCCAGACTCCCTGGCTGTGTCTCTGGGCGAGAGGG13810.10394744369074393No Hit
GTCTACGTAGTATGTCTTACCATCGCTAGAAATAACTGAGACCCACTCCAGCCCCTTCTCTGGAGCCTGGCGGAC13690.10304420739509663No Hit
GCACACAACAGAGGCAGTTCCAGATTTCAACTGCTCATCAGATGGCGGGA13580.10221624079075327No Hit
CCTCTGTTGTGTGCCTGCTGAATAACTTCTATCCCAGAGAGGCCAAAGTA13560.10206570140814537No Hit
GGTGAAAGATGAGCTGGAGGACCGCAATAGGGGTAGGTCCCCTGTGGAAA13410.10093665603858626No Hit
GTTGAAATCTGGAACTGCCTCTGTTGTGTGCCTGCTGAATAACTTCTATC13400.10086138634728233No Hit
GCCCAGCACTGGGATTCCGAGGTGTTTCCAATCGGTGATCAGCACTGAAC13320.10025922881685077No Hit
GAGTTTGTGTTTCTCGTAGTCTGCTTTGCTCAGCGTCAGGGTGCTGCTGAGGCTGTAGGTGCTGTCCTTGCTGTC13290.10003341974293896No Hit

[OK]Adapter Content

Adapter graph

[FAIL]Kmer Content

Kmer graph

SequenceCountPValueObs/Exp MaxMax Obs/Exp Position
CGTATGC3900.054.0637741
TCGTATG3950.053.3123140
GACTAGC3950.052.74167326
CTAGCGC4050.051.48792628
CACGAGA4050.051.38518521
GGTACGT303.729627E-451.1784468
ATCTCGT3850.050.91637837
GAGACTA4100.050.77765324
CTTATAC4250.050.585871
TAGCGCT4150.050.27000829
ACTAGCG4150.050.2207327
AGACTAG4150.050.18477625
TATACAC4550.049.4688873
ACGAGAC4300.049.19663622
ATGCCGT4400.048.87539344
CGAGACT4400.048.08577323
AGCGCTC4300.047.7408730
GCCGTCT4450.047.68183546
ACACATC4650.047.6752246
GTATGCC4450.047.4305842
Produced by FastQC (version 0.11.5)