FastQCFastQC Report
Sat 23 Apr 2022
EGAF00004684439

Summary

[OK]Basic Statistics

MeasureValue
FilenameEGAF00004684439
File typeConventional base calls
EncodingSanger / Illumina 1.9
Total Sequences1328556
Sequences flagged as poor quality0
Sequence length20-76
%GC55

[OK]Per base sequence quality

Per base quality graph

[OK]Per tile sequence quality

Per base quality graph

[OK]Per sequence quality scores

Per Sequence quality graph

[FAIL]Per base sequence content

Per base sequence content

[FAIL]Per sequence GC content

Per sequence GC content graph

[OK]Per base N content

N content graph

[WARN]Sequence Length Distribution

Sequence length distribution

[FAIL]Sequence Duplication Levels

Duplication level graph

[WARN]Overrepresented sequences

SequenceCountPercentagePossible Source
CTCCTGGGAGTTACCCGATTGGAGGGCGTTATCCACCTTCCACTGTACTTTGGCCTCTCTGGGATAGAAGTTATT53320.40133799403261883No Hit
CTCCTAAGCTGCTCATTTACTGGGCGTCTTCCCGGGAATCCGGGGTCCCT48450.36468165436759914No Hit
GTTTTATACAACTCCAACAATAAGAACTACTTAGCTTGGTACCAGCAGAA46740.351810537154625No Hit
CTCTAACACTCTCCCCTGTTGAAGCTCTTTGTGACGGGCGAGCTCAGGCC44950.3383372624112194No Hit
GGGGGGGGGGGGGGGGGGGGGGGGGGGGGGGGGGGGGGGGGGGGGGGGGG43720.32907909038083455No Hit
ATATTATAGTAATCCACCACACACTTTTGGCCAGGGGACCAAGCTGGAGCTCAAACGAACTGTGGCTGCACCAT42110.31696067008089984No Hit
CTTTTGGCCAGGGGACCAAGCTGGAGCTCAAACGAACTGTGGCTGCACCA40510.30491751947226914No Hit
GCTTAGGAGGCTGTCCTGGTTTCTGCTGGTACCAAGCTAAGTAGTTCTTATTGTTGGAGTTGTATAAAACACT37970.2857990178810679No Hit
GTCATAGGTGGTCAGGTCTGTGACCAGGCAGGTCAACTTGGTGGACTTGG37600.28301403930282204No Hit
CTATAATATTGCTGACAGTAATAAACTGCCACATCTTCAGCCTGCAGGCTGCTGATGGTGAGAGTGAAATCTGTC36220.272626821902878No Hit
CTCCCAGGAGAGTGTCACAGAGCAGGACAGCAAGGACAGCACCTACAGCC35230.26517512246378777No Hit
GGCTGGAACTGAGGAGCAGGTGGGGGCACTTCTCCCTCTAACACTCTCCC29110.21911007138577526No Hit
GTCCTGCTCTGTGACACTCTCCTGGGAGTTACCCGATTGGAGGGCGTTATCCACCTTCCACTGTACTTTGGCCT28870.2173035987944806No Hit
CAACAGAGGCAGTTCCAGATTTCAACTGCTCATCAGATGGCGGGAAGATG28360.2134648445379796No Hit
GTGCAGGGCCACCCCCTTGGGCCGGGAGATGGTCTGCTTCAGTGGCGAGGGCAGGTCTGTGTGGGTCACGGTGCA27860.20970135997278247No Hit
GTACAGTGGAAGGTGGATAACGCCCTCCAATCGGGTAACTCCCAGGAGAG26900.20247546960760401No Hit
CAATAATACATGGCCGTGTCCTCGGCTCTCAGGCTGTTCATTTGAAGATA26610.20029264855978973No Hit
GCGTCAGGGTGCTGCTGAGGCTGTAGGTGCTGTCCTTGCTGTCCTGCTCT26590.20014210917718186No Hit
GGCGTAGAGTTTGTGTTTCTCGTAGTCTGCTTTGCTCAGCGTCAGGGTGC26290.19788401843806358No Hit
GGCCAAAAGTGTGTGGTGGATTACTATAATATTGCTGACAGTAATAAACTGCCACATCTTCAGCCTGCAGGCTG26060.19615281553807293No Hit
GTAATAAACTGCCACATCTTCAGCCTGCAGGCTGCTGATGGTGAGAGTGAAATCTGTCCCAGACCCGCTGGCA25680.19329256726852312No Hit
GTGTTGCAGACCCAGGTCTTCATTTCTCTGTTGCTCTGGATCTCTGGTGC25030.18840003733376687No Hit
GTCTTCATCTTCCCGCCATCTGATGAGCAGTTGAAATCTGGAACTGCCTCTGTTGTGTGCCTGCTGAATAACTT24500.18441074369465796No Hit
GTTTATTACTGTCAGCAATATTATAGTAATCCACCACACACTTTTGGCCA23730.17861497746425442No Hit
GGATGGGAGGGGGTCAGGCTGGAACTGAGGAGCAGGTGGGGGCACTTCTCCCTCTAACACTCTCCCCTGTTGAAG23020.17327082938167454No Hit
GAGCAGGACAGCAAGGACAGCACCTACAGCCTCAGCAGCACCCTGACGCT22510.1694320751251735No Hit
GTCTCCAGACTCCCTGGCTGTGTCTCTGGGCGAGAGGGCCACCATCAACTGCAAGTCCAGCCAGAGTGTTTTATA22470.16913099635995774No Hit
GTGGCTGCACCATCTGTCTTCATCTTCCCGCCATCTGATGAGCAGTTGAAATCTGGAACTGCCTCTGTTGTGTGC21980.16544278148606456No Hit
GGTGTAGGTCTCCCCCGTGTTCCATTCCTCTTCGGACACGGTCAGGATGCTGTGGGCGAAGTACCGGCCTGGGGC21800.16408792704259362No Hit
CCACTGTACTTTGGCCTCTCTGGGATAGAAGTTATTCAGCAGGCACACAACAGAGGCAGTTCCAGATTTCAACTG21750.1637115785860739No Hit
TGCACACACAGAGCGGCCAGCCGCCCCGAGCCTGTGGGCAGGCCAGCAGG21270.16009863340348468No Hit
CAATAGGGGTAGGTCCCCTGTGGAAAAAGGGTCAGAGGCCAAAGGATGGGAGGGGGTCAGGCTGGAACTGAGGAG21060.15851796988610192No Hit
GTATTATTGTGCGAGACACTCTGGAAGCAGCTGGGTTGACTACTGGGGCC20850.1569373063687191No Hit
CTCTGGGATAGAAGTTATTCAGCAGGCACACAACAGAGGCAGTTCCAGAT20710.15588353069046393No Hit
GGCTGTAGGTGCTGTCCTTGCTGTCCTGCTCTGTGACACTCTCCTGGGAGTTACCCGATTGGAGGGCGTTATCCA20570.15482975501220875No Hit
GCCCTGCACAGGCCCGATGTCTACTTGCTGCCACCAGCCCGGGAGCAGCT19870.14956087662093281No Hit
CTCTACGCCTGCGAAGTCACCCATCAGGGCCTGAGCTCGCCCGTCACAAA19570.14730278588181456No Hit
AACCAGGACAGCCTCCTAAGCTGCTCATTTACTGGGCGTCTTCCCGGGAATCCGGGGTCCCTGACCGATTCAGTG19440.1463242798948633No Hit
CTCCTGTGCAGCCTCTGGGTTCGCCGTCAGTGACATGTTCATGAGCTGGGTCCGCCAGGCTCCAGAGAAGGGG19420.1461737405122554No Hit
CCCTGACGCTGAGCAAAGCAGACTACGAGAAACACAAACTCTACGCCTGCGAAGTCACCCATCAGGGCCTGAGCT19110.1438403800818332No Hit
CTCCCTGGCTGTGTCTCTGGGCGAGAGGGCCACCATCAACTGCAAGTCCAGCCAGAGTGTTTTATACAACTCCAA19050.14338876193400954No Hit
GTCCCTGACCGATTCAGTGCCAGCGGGTCTGGGACAGATTTCACTCTCAC18460.13894785014707697No Hit
ATCCCAGAGAGGCCAAAGTACAGTGGAAGGTGGATAACGCCCTCCAATCG18050.13586179280361535No Hit
ATTCAGTGCCAGCGGGTCTGGGACAGATTTCACTCTCACCATCAGCAGCC18040.1357865231123114No Hit
GCTCATTTACTGGGCGTCTTCCCGGGAATCCGGGGTCCCTGACCGATTCAGTGCCAGCGGGTCTGGGACAGATTT17950.13510909589057593No Hit
GTGCAGCCACAGTTCGTTTGAGCTCCAGCTTGGTCCCCTGGCCAAAAGTGTGTGGTGGATTACTATAATATTG17950.13510909589057593No Hit
GTATAAAACACTCTGGCTGGACTTGCAGTTGATGGTGGCCCTCTCGCCCAGAGACACAGCCAGGGAGTCTGGAGA17880.13458220805144833No Hit
GAGTGAAATCTGTCCCAGACCCGCTGGCACTGAATCGGTCAGGGACCCCG17040.1282595539819172No Hit
GTTGTGTGCCTGCTGAATAACTTCTATCCCAGAGAGGCCAAAGTACAGTG17040.1282595539819172No Hit
CCCTGGCTGTGTCTCTGGGCGAGAGGGCCACCATCAACTGCAAGTCCAGC16960.12765739645148566No Hit
GATTTCACTCTCACCATCAGCAGCCTGCAGGCTGAAGATGTGGCAGTTTATTACTGTCAGCAATATTATAGTAAT16950.12758212676018174No Hit
GATGAAGACAGATGGTGCAGCCACAGTTCGTTTGAGCTCCAGCTTGGTCC16760.12615200262540682No Hit
GCACTGAACACAGAGGACTCACCATGGAGTTTTGGCTGAGCTGGGTTTTC16550.12457133910802405No Hit
ACCTATGACAGCGTGACCATCTCCTGGACCCGCCAGAATGGCGAAGCTGT16460.12389391188628858No Hit
GTCCTGAGAGGGGGCAAGTACGCAGCCACCTCACAGGTGCTGCTGCCTTC16280.12253905744281761No Hit
CCTACAGCCTCAGCAGCACCCTGACGCTGAGCAAAGCAGACTACGAGAAACACAAACTCTACGCCTGCGAAGTCA16260.12238851806020974No Hit
GTCCCAGACCCGCTGGCACTGAATCGGTCAGGGACCCCGGATTCCCGGGA16170.12171109083847426No Hit
GTCCTTGCTGTCCTGCTCTGTGACACTCTCCTGGGAGTTACCCGATTGGAGGGCGTTATCCACCTTCCACTGTA16100.12118420299934665No Hit
GGCCCTGATGGGTGACTTCGCAGGCGTAGAGTTTGTGTTTCTCGTAGTCTGCTTTGCTCAGCGTCAGGGTGCTG15770.11870030318631658No Hit
GGCCAAAGGATGGGAGGGGGTCAGGCTGGAACTGAGGAGCAGGTGGGGGCACTTCTCCCTCTAACACTCTCCC15570.11719490936023774No Hit
CTCCATGTGTGTCCCCGATCAAGACACAGCCATCCGGGTCTTCGCCATCCCCCCATCCTTTGCCAGCATCTTCCT15200.11440993078199188No Hit
GTACTTTGGCCTCTCTGGGATAGAAGTTATTCAGCAGGCACACAACAGAGGCAGTTCCAGATTTCAACTGCTCAT15180.114259391399384No Hit
GACCGATTCAGTGCCAGCGGGTCTGGGACAGATTTCACTCTCACCATCAG15120.11380777325156034No Hit
GCTTTGCTCAGCGTCAGGGTGCTGCTGAGGCTGTAGGTGCTGTCCTTGCT14760.11109806436461842No Hit
GTCAGGGACCCCGGATTCCCGGGAAGACGCCCAGTAAATGAGCAGCTTAGGAGGCTGTCCTGGTTTCTGCTGGTA14580.10974320992114747No Hit
GTATGTGACCAGCGCCCCAATGCCTGAGCCCCAGGCCCCAGGCCGGTACTTCGCCCACAGCATCCTGACCGTGTC14480.10899051300810804No Hit
GTAAATGAGCAGCTTAGGAGGCTGTCCTGGTTTCTGCTGGTACCAAGCTA14450.10876470393419621No Hit
GTACAGGGTGGGTTTACCGGTGGACTTGTCCACGGTCCTCTCGGTGACCCTGTTGGGCAGGGCCTCATGGGCCAC14430.10861416455158834No Hit
GTCCTGGTTTCTGCTGGTACCAAGCTAAGTAGTTCTTATTGTTGGAGTTG14380.10823781609506863No Hit
TCTTCAGCCTGCAGGCTGCTGATGGTGAGAGTGAAATCTGTCCCAGACCCGCTGGCACTGAATCGGTCAGGGACC14350.1080120070211568No Hit
CAGTAATAAACTGCCACATCTTCAGCCTGCAGGCTGCTGATGGTGAGAGTGAAATCTGTCCCAGACCCGCTGGCA14210.10695823134290161No Hit
GGATGGGGGGATGGCGAAGACCCGGATGGCTGTGTCTTGATCGGGGACAC14170.10665715257768586No Hit
GGATCTCTGGTGCCTACGGGGCCATCGTGATGACCCAGTCTCCAGACTCCCTGGCTGTGTCTCTGGGCGAGAGGG14070.10590445566464643No Hit
GGCAGGGGCAGCAAGATGGTGTTGCAGACCCAGGTCTTCATTTCTCTGTT14030.10560337689943067No Hit
GTGTCACAGAGCAGGACAGCAAGGACAGCACCTACAGCCTCAGCAGCACC14000.10537756782551884No Hit
CTACGTAGACTCCGTGAAGGGCCGATTCACCATCTCCAGAGACAGTTCCAAGAACACTCTCTATCTTCAAATGAA13940.1049259496776952No Hit
CTTCTATCCCAGAGAGGCCAAAGTACAGTGGAAGGTGGATAACGCCCTCCAATCGGGTAACTCCCAGGAGAGTGT13870.10439906183856759No Hit
CCTCTGTTGTGTGCCTGCTGAATAACTTCTATCCCAGAGAGGCCAAAGTA13700.10311947708640057No Hit
GTCTCTGGGCGAGAGGGCCACCATCAACTGCAAGTCCAGCCAGAGTGTTTTATACAACTCCAACAATAAGAACTA13410.10093665603858626No Hit
GGTCACATACTTCTCCGGGGACAAGGGCTGCCCCCTCTGCATCCACTGCACGAAGACGTCCGCGGGAGAGAAGCC13340.10040976819945867No Hit

[OK]Adapter Content

Adapter graph

[FAIL]Kmer Content

Kmer graph

SequenceCountPValueObs/Exp MaxMax Obs/Exp Position
GGTATCA10400.045.5154651
GTGGTAT3200.043.632631
TGGTATC3150.043.2066732
GTATCAA24250.037.4953161
CCGTTAA502.0105497E-434.05088816
TATAAGG400.002779007434.0252572
GCTATAC400.00278003334.0226943
TATCAAC28350.032.1649672
AATGGTA3050.032.1264970
ATCAACG29950.030.3307483
GCTCGGG4150.029.9073170
TCAACGC30450.029.8327084
AACGCAG30550.029.5200966
CAACGCA30800.029.497035
GTGAGCG7350.029.32908270
ACAGACG10850.028.89903870
GACAGGT1800.028.3789481
GCCGTTA605.857277E-428.373615
GGTACCG605.874074E-428.3597156
ACGAACC1004.2544525E-728.12637952
Produced by FastQC (version 0.11.5)