FastQCFastQC Report
Wed 27 Apr 2022
EGAF00004684440

Summary

[OK]Basic Statistics

MeasureValue
FilenameEGAF00004684440
File typeConventional base calls
EncodingSanger / Illumina 1.9
Total Sequences832127
Sequences flagged as poor quality0
Sequence length20-76
%GC55

[OK]Per base sequence quality

Per base quality graph

[OK]Per tile sequence quality

Per tile quality graph

[OK]Per sequence quality scores

Per Sequence quality graph

[FAIL]Per base sequence content

Per base sequence content

[FAIL]Per sequence GC content

Per sequence GC content graph

[OK]Per base N content

N content graph

[WARN]Sequence Length Distribution

Sequence length distribution

[FAIL]Sequence Duplication Levels

Duplication level graph

[WARN]Overrepresented sequences

SequenceCountPercentagePossible Source
GCCCAAGGCTGCCCCCTCGGTCACTCTCTTCCCGCCCTCCTCTGAGGAGC26080.3134136976687453No Hit
GCCTTGGGCTGACCTAGGACGGTCAGCCTGGTCCCTCCGCCGAAAAGCAC22010.26450289438991886No Hit
CCTATGAGGTGACTCAGCCGCTCTCAGTGTCAGTGGCCCTGGGACAGACG21850.2625801109686382No Hit
CTTTACAGGTTCTGTGGCCTCCTATGAGGTGACTCAGCCGCTCTCAGTGT21150.25416793350053535No Hit
CCTCTGAGGAGCTTCAAGCCAACAAGGCCACACTGGTGTGTCTCATAAGT20500.24635662585158277No Hit
GTGTGGTGGTCTCCACTCCCGCCTTGACGGGGCTGCTATCTGCCTTCCAG18580.223283224796215No Hit
GGCCTGGACCGCTCTCCTTCTGAGCCTCCTTGCTCACTTTACAGGTTCTG18400.22112009344727426No Hit
GGATTACCTGTGGGGGCAATGACATTGGAGATAAAAATGTGCACTGGTACCAGCAGAAGCCAGGCCAGGCCC18100.21751487453237306No Hit
GTGCAGGGAGAAGGGCTTGATGCCTTGGGGTGGGAGGAGAGACCCCTCCCCTGGGATCCTGCAGCTCTAGTCTCC17830.21427017750896196No Hit
CTTATACACATCTCCGAGCCCACGAGACATCTCAGGATCTCGTATGCCGT17690.21258774201534142No Hit
GGGTTTATTGAGTGCAGGGAGAAGGGCTTGATGCCTTGGGGTGGGAGGAGAGACCCCTCCCCTGGGATCCTGCAG17500.21030443670257062No Hit
CCTATGAACATTCTGTAGGGGCCACTGTCTTCTCCACGGTGCTCCCTTCATGCGTGACCTGGCAGCTGTAGCTT16870.20273347698127808No Hit
GACCTAGGACGGTCAGCCTGGTCCCTCCGCCGAAAAGCACAGAGGCAGTG16710.20081069355999745No Hit
CCTCAGAGGAGGGCGGGAAGAGAGTGACCGAGGGGGCAGCCTTGGGCTGACCTAGGACGGTCAGCCTGGTCCCTC15180.18242407709400127No Hit
GGTTTATTGAGTGCAGGGAGAAGGGCTTGATGCCTTGGGGTGGGAGGAGAGACCCCTCCCCTGGGATCCTGCAG15150.18206355520251116No Hit
CCCCTGTGCTGGTCATTCATAGGGACAGCAGCCGGCCCTCTGGGATCCCT15070.18110216349187083No Hit
TCCTAGGTCAGCCCAAGGCTGCCCCCTCGGTCACTCTCTTCCCGCCCTCCTCTGAGGAGCTTCAAGCCAACAAGG14960.1797802498897404No Hit
GTCTTCTCCACGGTGCTCCCTTCATGCGTGACCTGGCAGCTGTAGCTTCT14810.17797764043228978No Hit
GTGCTCCCTTCATGCGTGACCTGGCAGCTGTAGCTTCTGTGGGACTTCCACTGCTCAGGCGTCAGGCTCAGGTAG14430.17341102980674825No Hit
GTCCTAGGTCAGCCCAAGGCTGCCCCCTCGGTCACTCTCTTCCCGCCCTC14390.17293033395142807No Hit
GAGAAGGGCTTGATGCCTTGGGGTGGGAGGAGAGACCCCTCCCCTGGGAT14050.1688444191812067No Hit
GCACAGGGGCCTGGCCTGGCTTCTGCTGGTACCAGTGCACATTTTTATCT13590.16331641684502488No Hit
GACTATTACTGTCAAATATGGTACGTCAACACTGCCTCTGTGCTTTTCGG13510.16235502513438455No Hit
GTTTGGAGGGTGTGGTGGTCTCCACTCCCGCCTTGACGGGGCTGCTATCTGCCTTCCAGGCCACTGTCACGGCTC13200.15862963225565327No Hit
GCCATGGCCTGGACCGCTCTCCTTCTGAGCCTCCTTGCTCACTTTACAGGTTCTGTGGCCTCCTATGAGGTGACT12930.15538493523224217No Hit
GTAATAGTCAGCCTCATCCCCGGCTTGGGCTCTAGTGATGGTCAGGGTGG12620.15165954235351095No Hit
CTCTCTTCCCGCCCTCCTCTGAGGAGCTTCAAGCCAACAAGGCCACACTGGTGTGTCTCATAAGTGACTTCTACC12320.14805432343860972No Hit
TTCATAGGGACAGCAGCCGGCCCTCTGGGATCCCTGACCGATTCTCTGGCTCCAACTCGGGGAATATGGCCACC12190.14649206190881922No Hit
GCTCAGGCGTCAGGCTCAGGTAGCTGCTGGCCGCGTACTTGTTGTTGCTTTGTTTGGAGGGTGTGGTGGTCTCCA12130.14577101812583898No Hit
CTTCATGCGTGACCTGGCAGCTGTAGCTTCTGTGGGACTTCCACTGCTCAGGCGTCAGGCTCAGGTAGCTGCTGG11950.14360788677689826No Hit
CCACCACACCCTCCAAACAAAGCAACAACAAGTACGCGGCCAGCAGCTAC11710.14072371164497727No Hit
GTCTCTGGTGGCTCCATCAGTCCTTACTACTGGAGCTGGATCCGGCAGCC11530.13856058029603655No Hit
GAGTTTATTCAGGGGTGGGGACAGGCGGGCGGCTCAGTAGCAGGTGCCGT11460.13771936254922626No Hit
GCCTTGGGGTGGGAGGAGAGACCCCTCCCCTGGGATCCTGCAGCTCTAGT11290.13567640516411555No Hit
GCTCAGTAGCAGGTGCCGTCCACCTCCGCCATGACAACAGACACATTGAC11150.133993969670495No Hit
CCCTTCATGCGTGACCTGGCAGCTGTAGCTTCTGTGGGACTTCCACTGCTCAGGCGTCAGGCTCAGGTAGCTG10820.13002822886410367No Hit
CACCAGTGTGGCCTTGTTGGCTTGAAGCTCCTCAGAGGAGGGCGGGAAGAGAGTGACCGAGGGGGCAGCCTTGGG10590.12726422769601273No Hit
GTGTTGACGTACCATATTTGACAGTAATAGTCAGCCTCATCCCCGGCTTG10430.1253414442747321No Hit
GCTCTAGTCTCCCGTGGTGGGGGTGAGGGTTGAGAACCTATGAACATTCT10380.1247405744555819No Hit
GTATGCTGGTCACAGCGAAGGTGGTGGTGCCCTGGCTGGGCTCCTGCCGG9970.11981344193855024No Hit
GGCTCAGGTAGCTGCTGGCCGCGTACTTGTTGTTGCTTTGTTTGGAGGGTGTGGTGGTCTCCACTCCCGCCTTGA9800.11777048455343957No Hit
CTTATGAGACACACCAGTGTGGCCTTGTTGGCTTGAAGCTCCTCAGAGGA9650.11596787509598895No Hit
ACACTGGTGTGTCTCATAAGTGACTTCTACCCGGGAGCCGTGACAGTGGC9500.11416526563853835No Hit
GTCACATCCTGGCTGGGATTCGTGTAGTGCTTCACGTGGCATGTCACGGA9360.11248283014491779No Hit
CCCCTGGGATCCTGCAGCTCTAGTCTCCCGTGGTGGGGGTGAGGGTTGAG9140.10983900294065689No Hit
GCTATCTGCCTTCCAGGCCACTGTCACGGCTCCCGGGTAGAAGTCACTTA9080.10911795915767666No Hit
TCACTAGAGCCCAAGCCGGGGATGAGGCTGACTATTACTGTCAAATATGG9010.10827674141086636No Hit
GGCCAGGCCCCTGTGCTGGTCATTCATAGGGACAGCAGCCGGCCCTCTGG8990.10803639348320629No Hit
GAAGTAAAGGGCCGTGTCCGCAGCGGTCACAGAGCTCAGCTTCAGGGAGAACTGGTTCTTGGACGTGTCTACTGA8910.10707500177256596No Hit
ACCTAGGACGGTCAGCCTGGTCCCTCCGCCGAAAAGCACAGAGGCAGTGT8770.1053925662789454No Hit
GGTGTGTCTCATAAGTGACTTCTACCCGGGAGCCGTGACAGTGGCCTGGA8750.10515221835128531No Hit
ATTCAGGGGTGGGGACAGGCGGGCGGCTCAGTAGCAGGTGCCGTCCACCT8690.10443117456830507No Hit
CTCTCCTTCTGAGCCTCCTTGCTCACTTTACAGGTTCTGTGGCCTCCTATGAGGTGACTCAGCCGCTCTCAGTGT8580.10310926096617463No Hit
CCCTGGGACAGACGGCCAGGATTACCTGTGGGGGCAATGACATTGGAGAT8440.10142682547255406No Hit
GCTGTAGCTTCTGTGGGACTTCCACTGCTCAGGCGTCAGGCTCAGGTAGC8380.10070578168957384No Hit

[OK]Adapter Content

Adapter graph

Produced by FastQC (version 0.11.9)