FastQCFastQC Report
Sat 23 Apr 2022
EGAF00004684442

Summary

[OK]Basic Statistics

MeasureValue
FilenameEGAF00004684442
File typeConventional base calls
EncodingSanger / Illumina 1.9
Total Sequences880435
Sequences flagged as poor quality0
Sequence length20-76
%GC54

[OK]Per base sequence quality

Per base quality graph

[OK]Per tile sequence quality

Per base quality graph

[OK]Per sequence quality scores

Per Sequence quality graph

[FAIL]Per base sequence content

Per base sequence content

[WARN]Per sequence GC content

Per sequence GC content graph

[OK]Per base N content

N content graph

[WARN]Sequence Length Distribution

Sequence length distribution

[FAIL]Sequence Duplication Levels

Duplication level graph

[WARN]Overrepresented sequences

SequenceCountPercentagePossible Source
CTCCTGGGAGTTACCCGATTGGAGGGCGTTATCCACCTTCCACTGTACTTTGGCCTCTCTGGGATAGAAGTTATT39830.4523900117555527No Hit
CTCTAACACTCTCCCCTGTTGAAGCTCTTTGTGACGGGCGAGCTCAGGCC38920.44205421183846616No Hit
ATATTATAGTACTCCTCCGGCTTTCGGCGGAGGGACCAAGGTGGAGATCAGACGAACTGTGGCTGCACCAT33800.38390113977749635No Hit
GGCGTAGACTTTGTGTTTCTCGTAGTCTGCTTTGCTCAGCGTCAGGGTGC27890.316775230425869No Hit
GGCTGGAACTGAGGAGCAGGTGGGGGCACTTCTCCCTCTAACACTCTCCC25900.29417276687092175No Hit
CTATAATATTGCTGACAGTAATAAACCGCCACATCCTCAGCCTGCAGGCTGCTGATGCTGAGAGTGAAATCTGTC24830.28201968345193No Hit
CTCCCAGGAGAGTGTCACAGAGCAGGACAGCAAGGACAGCACCTACAGCC24690.28042956038776284No Hit
CTTTTATATAGTTCCAACAACAAGAACTACTTAGCTTGGTACCAGCAGAAAGTTGGACAGCCTCCTAAGTTGCT23310.2647554901838296No Hit
TTATAGTACTCCTCCGGCTTTCGGCGGAGGGACCAAGGTGGAGATCAGACGAACTGTGGCTGCACCATCTGTCTT22980.26100734296114986No Hit
GGATGGGAGGGGGTCAGGCTGGAACTGAGGAGCAGGTGGGGGCACTTCTCCCTCTAACACTCTCCCCTGTTGAAG22560.25623697376864846No Hit
CAACAGAGGCAGTTCCAGATTTCAACTGCTCATCAGATGGCGGGAAGATG22330.2536246287346596No Hit
CAATAGGGGTAGGTCCCCTGTGGAAAAAGGGTCAGAGGCCAAAGGATGGGAGGGGGTCAGGCTGGAACTGAGGAG22240.2526024067648378No Hit
GTCTACGCCTGCGAAGTCACCCATCAGGGCCTGAGCTCGCCCGTCACAAA22230.25248882654596877No Hit
GTCCTGCTCTGTGACACTCTCCTGGGAGTTACCCGATTGGAGGGCGTTATCCACCTTCCACTGTACTTTGGCCT21890.24862709910442No Hit
GTACAGTGGAAGGTGGATAACGCCCTCCAATCGGGTAACTCCCAGGAGAG20860.23692833656090453No Hit
GCGTCAGGGTGCTGCTGAGGCTGTAGGTGCTGTCCTTGCTGTCCTGCTCT20450.23227154758727217No Hit
GTACTATAATATTGCTGACAGTAATAAACCGCCACATCCTCAGCCTGCAGGCTGCTGATGCTGAGAGTGAAAT20090.22818265970798524No Hit
CTCCCTGACTGTGTCTCTGGGCGAGAGGGCCACCATCAACTGCAAGTCAAGCCAGCGTCTTTTATATAGTTCCAA19860.22557031467399638No Hit
GTCTTCATCTTCCCGCCATCTGATGAGCAGTTGAAATCTGGAACTGCCTCTGTTGTGTGCCTGCTGAATAACTT18850.21409871256821913No Hit
ACTTAGGAGGCTGTCCAACTTTCTGCTGGTACCAAGCTAAGTAGTTCTTGTTGTTGGAACTATATAAAAGACG17890.2031950115567873No Hit
CCACTGTACTTTGGCCTCTCTGGGATAGAAGTTATTCAGCAGGCACACAACAGAGGCAGTTCCAGATTTCAACTG17750.20160488849262012No Hit
GTAATAAACCGCCACATCCTCAGCCTGCAGGCTGCTGATGCTGAGAGTGAAATCTGTCCCAGACCCGCTGGCA17500.19876538302089308No Hit
GTGTTGCAGACCCAGGTCTTCATTTCTCTGTTGCTCTGGATCTCTGGTGG16940.1924048907642245No Hit
CTCTGGGATAGAAGTTATTCAGCAGGCACACAACAGAGGCAGTTCCAGAT16440.1867258798207704No Hit
GTTTATTACTGTCAGCAATATTATAGTACTCCTCCGGCTTTCGGCGGAGGGACCAAGGTGGAGATCAGACGAA15720.1785481040621965No Hit
TCCTCAGCCTGCAGGCTGCTGATGCTGAGAGTGAAATCTGTCCCAGACCCGCTGGCACTGAATCGGTCAGGGACC15670.1779802029678511No Hit
CTCCTAAGTTGCTCTTTTACTGGGCATCTATTCGGGAATCCGGGGTCCCT15450.17548143815273132No Hit
GTCTCCAGACTCCCTGACTGTGTCTCTGGGCGAGAGGGCCACCATCAACT15350.1743456359640405No Hit
GGCCAAAGGATGGGAGGGGGTCAGGCTGGAACTGAGGAGCAGGTGGGGGCACTTCTCCCTCTAACACTCTCCC15270.17343699421308784No Hit
GGCTGTAGGTGCTGTCCTTGCTGTCCTGCTCTGTGACACTCTCCTGGGAGTTACCCGATTGGAGGGCGTTATCCA14850.1686666250205864No Hit
GTGGCTGCACCATCTGTCTTCATCTTCCCGCCATCTGATGAGCAGTTGAAATCTGGAACTGCCTCTGTTGTGTGC14750.16753082283189558No Hit
CCCTGACTGTGTCTCTGGGCGAGAGGGCCACCATCAACTGCAAGTCAAGC14440.16400983604695407No Hit
ATCCCAGAGAGGCCAAAGTACAGTGGAAGGTGGATAACGCCCTCCAATCG14350.16298761407713233No Hit
GAGCAGGACAGCAAGGACAGCACCTACAGCCTCAGCAGCACCCTGACGCT14290.16230613276391784No Hit
GTCCCTGACCGATTCAGTGCCAGCGGGTCTGGGACAGATTTCACTCTCAG14180.16105675035635794No Hit
GTCCCAGACCCGCTGGCACTGAATCGGTCAGGGACCCCGGATTCCCGAAT14040.1594666272921908No Hit
ATTCAGTGCCAGCGGGTCTGGGACAGATTTCACTCTCAGCATCAGCAGCC14000.15901230641671446No Hit
ATATAAAAGACGCTGGCTTGACTTGCAGTTGATGGTGGCCCTCTCGCCCAGAGACACAGTCAGGGAGTCTGGAGA13970.1586715657601072No Hit
GAGTGAAATCTGTCCCAGACCCGCTGGCACTGAATCGGTCAGGGACCCCG13560.15401477678647488No Hit
GTTGTGTGCCTGCTGAATAACTTCTATCCCAGAGAGGCCAAAGTACAGTG13370.1518567526279623No Hit
GCTTTGCTCAGCGTCAGGGTGCTGCTGAGGCTGTAGGTGCTGTCCTTGCT13300.15106169109587875No Hit
GACCGATTCAGTGCCAGCGGGTCTGGGACAGATTTCACTCTCAGCATCAG13240.15038020978266425No Hit
GGCCCTGATGGGTGACTTCGCAGGCGTAGACTTTGTGTTTCTCGTAGTCTGCTTTGCTCAGCGTCAGGGTGCTG13140.14924440759397345No Hit
GTTGAAGCTCTTTGTGACGGGCGAGCTCAGGCCCTGATGGGTGACTTCGC12470.14163453292974496No Hit
CTTATACACATCTCCGAGCCCACGAGACCGTACTAGATCTCGTATGCCGT12370.14049873074105412No Hit
GTGCAGCCACAGTTCGTCTGATCTCCACCTTGGTCCCTCCGCCGAAAGCC12120.13765922526932708No Hit
GGCAGGGGCAGCAAGATGGTGTTGCAGACCCAGGTCTTCATTTCTCTGTT11980.13606910220515994No Hit
GATTTCACTCTCAGCATCAGCAGCCTGCAGGCTGAGGATGTGGCGGTTTATTACTGTCAGCAATATTATAGTACT11900.1351604604542073No Hit
GTACTTTGGCCTCTCTGGGATAGAAGTTATTCAGCAGGCACACAACAGAGGCAGTTCCAGATTTCAACTGCTCAT11660.13243453520134935No Hit
GTAATATACGGCTGTGTCCTCGATTTTCAGGCTGTTCATCTGCAGATACA11360.12902712863527688No Hit
GGCTGAGGATGTGGCGGTTTATTACTGTCAGCAATATTATAGTACTCCTC11260.12789132644658607No Hit
GTCCTTGCTGTCCTGCTCTGTGACACTCTCCTGGGAGTTACCCGATTGGAGGGCGTTATCCACCTTCCACTGTA11160.12675552425789524No Hit
GTCAGGGACCCCGGATTCCCGAATAGATGCCCAGTAAAAGAGCAACTTAGGAGGCTGTCCAACTTTCTGCTGGTA11090.12596046272581166No Hit
AACTGTGGCTGCACCATCTGTCTTCATCTTCCCGCCATCTGATGAGCAGT11060.1256197220692044No Hit
GTGGAAAAAGGGTCAGAGGCCAAAGGATGGGAGGGGGTCAGGCTGGAACTGAGGAGCAGGTGGGGGCACTTCTCC11040.12539256163146625No Hit
CCCCTGTTGAAGCTCTTTGTGACGGGCGAGCTCAGGCCCTGATGGGTGACTTCGCAGGCGTAGACTTTGTGTTT10970.12459750009938268No Hit
CTACTTAGCTTGGTACCAGCAGAAAGTTGGACAGCCTCCTAAGTTGCTCTTTTACTGGGCATCTATTCGGGAATC10950.12437033966164453No Hit
GTTGAAATCTGGAACTGCCTCTGTTGTGTGCCTGCTGAATAACTTCTATC10880.12357527812956096No Hit
GGGTAGGTCCCCTGTGGAAAAAGGGTCAGAGGCCAAAGGATGGGAGGGGG10850.12323453747295371No Hit
CTTCTATCCCAGAGAGGCCAAAGTACAGTGGAAGGTGGATAACGCCCTCCAATCGGGTAACTCCCAGGAGAGTGT10710.12164441440878657No Hit
GAGCAAAGCAGACTACGAGAAACACAAAGTCTACGCCTGCGAAGTCACCC10460.11880490893705951No Hit
GAATCGGTCAGGGACCCCGGATTCCCGAATAGATGCCCAGTAAAAGAGCAACTTAGGAGGCTGTCCAACTTTCTG10450.11869132871819045No Hit
CAGTAATAAACCGCCACATCCTCAGCCTGCAGGCTGCTGATGCTGAGAGTGAAATCTGTCCCAGACCCGCTGGCA10400.11812342762384503No Hit
CCGCAATAGGGGTAGGTCCCCTGTGGAAAAAGGGTCAGAGGCCAAAGGAT10370.11778268696723779No Hit
CTTTGTGACGGGCGAGCTCAGGCCCTGATGGGTGACTTCGCAGGCGTAGA10360.1176691067483687No Hit
GCACACAACAGAGGCAGTTCCAGATTTCAACTGCTCATCAGATGGCGGGA10170.11551108258985615No Hit
ACCTTCCACTGTACTTTGGCCTCTCTGGGATAGAAGTTATTCAGCAGGCA10140.1151703419332489No Hit
CCTCTGTTGTGTGCCTGCTGAATAACTTCTATCCCAGAGAGGCCAAAGTA10100.11471602105777258No Hit
GAACTGAGGAGCAGGTGGGGGCACTTCTCCCTCTAACACTCTCCCCTGTT10020.11380737930681993No Hit
GGTGAAAGATGAGCTGGAGGACCGCAATAGGGGTAGGTCCCCTGTGGAAA9980.11335305843134359No Hit
GTATGCTGGTCACAGCGAAGGTGGTGGTGCCCTGGCTGGGCTCCTGCCGG9970.1132394782124745No Hit
GCCTCCTAAGTTGCTCTTTTACTGGGCATCTATTCGGGAATCCGGGGTCCCTGACCGATTCAGTGCCAGCGGGT9930.11278515733699819No Hit
AAGTTGGACAGCCTCCTAAGTTGCTCTTTTACTGGGCATCTATTCGGGAATCCGGGGTCCCTGACCGATTCAGTG9930.11278515733699819No Hit
TCCTTGGGGCTGAAGCCACGTGCCAGGCACGTCAGCGTCACCAGCTCGTT9910.11255799689926002No Hit
GTGGAAGGTGGATAACGCCCTCCAATCGGGTAACTCCCAGGAGAGTGTCACAGAGCAGGACAGCAAGGACAGCAC9890.11233083646152187No Hit
CTACGGGGACATCGTGATGACCCAGTCTCCAGACTCCCTGACTGTGTCTCTGGGCGAGAGGGCCACCATCAACTG9890.11233083646152187No Hit
GTCACATCCTGGCTGGGATTCGTGTAGTGCTTCACGTGGCATGTCACGGA9780.11108145405396197No Hit
GTAAAAGAGCAACTTAGGAGGCTGTCCAACTTTCTGCTGGTACCAAGCTA9750.11074071339735472No Hit
GTGCCAGCGGGTCTGGGACAGATTTCACTCTCAGCATCAGCAGCCTGCAGGCTGAGGATGTGGCGGTTTATTA9680.10994565186527114No Hit
GATGAAGACAGATGGTGCAGCCACAGTTCGTCTGATCTCCACCTTGGTCC9490.10778762770675858No Hit
CCTCCAACATTAGCATAATTAAAGCCAAGGAGGAGGAGGGGGGTGAGGTG9490.10778762770675858No Hit
CTTTTACTGGGCATCTATTCGGGAATCCGGGGTCCCTGACCGATTCAGTGCCAGCGGGTCTGGGACAGATTTCA9480.10767404748788952No Hit
GAGGAGGAGGGGGGTGAGGTGAAAGATGAGCTGGAGGACCGCAATAGGGG9380.1065382452991987No Hit
GCCTCTGTTGTGTGCCTGCTGAATAACTTCTATCCCAGAGAGGCCAAAGTACAGTGGAAGGTGGATAACGCCCTC9380.1065382452991987No Hit
GTCTCTGGGCGAGAGGGCCACCATCAACTGCAAGTCAAGCCAGCGTCTTTTATATAGTTCCAACAACAAGAACTA9260.10517528267276971No Hit
GTGCTGTCCTTGCTGTCCTGCTCTGTGACACTCTCCTGGGAGTTACCCGATTGGAGGGCGTTATCCACCTTCCA9200.10449380135955522No Hit
GACCAAGGTGGAGATCAGACGAACTGTGGCTGCACCATCTGTCTTCATCT9000.10222219698217358No Hit
GGATCTCTGGTGGCTACGGGGACATCGTGATGACCCAGTCTCCAGACTCCCTGACTGTGTCTCTGGGCGAGAGGG8980.10199503654443542No Hit
GCTCAGGCCCTGATGGGTGACTTCGCAGGCGTAGACTTTGTGTTTCTCGT8960.10176787610669727No Hit
GGACTGAACACAGAGGACTCACCATGGAGTTTGGGCTGAGCTGGATTTTC8880.10085923435574459No Hit

[OK]Adapter Content

Adapter graph

[WARN]Kmer Content

Kmer graph

SequenceCountPValueObs/Exp MaxMax Obs/Exp Position
TACGTAT350.005222655456.43092370
GTTATTA503.2347717E-452.6688670
GTCGTAT200.007445518451.5962239
GTGGTAT1450.049.061291
GGTATCA7200.046.579021
GTACGTA408.1489264E-443.672169
GACCGTA2200.043.17333626
TCGTATG2250.042.88988540
ACCGTAC2250.042.23555827
ATCTCGT1950.042.2014537
CGTACTA2300.041.36684829
CGTATGC2350.041.14088441
TGGTATC1750.040.634622
CTTATAC2350.040.362481
GTACTAG2400.039.6703630
TATACAC2400.039.5058823
GGTACGT400.001343016539.45546368
ACTAGAT2350.039.13889732
TTATACA2450.038.6996422
GTATCAA19200.038.639421
Produced by FastQC (version 0.11.5)