FastQCFastQC Report
Sat 23 Apr 2022
EGAF00004684443

Summary

[OK]Basic Statistics

MeasureValue
FilenameEGAF00004684443
File typeConventional base calls
EncodingSanger / Illumina 1.9
Total Sequences880435
Sequences flagged as poor quality0
Sequence length20-76
%GC54

[OK]Per base sequence quality

Per base quality graph

[OK]Per tile sequence quality

Per base quality graph

[OK]Per sequence quality scores

Per Sequence quality graph

[FAIL]Per base sequence content

Per base sequence content

[WARN]Per sequence GC content

Per sequence GC content graph

[OK]Per base N content

N content graph

[WARN]Sequence Length Distribution

Sequence length distribution

[FAIL]Sequence Duplication Levels

Duplication level graph

[WARN]Overrepresented sequences

SequenceCountPercentagePossible Source
CTCCTGGGAGTTACCCGATTGGAGGGCGTTATCCACCTTCCACTGTACTTTGGCCTCTCTGGGATAGAAGTTATT39160.44478013709132413No Hit
ATATTATAGTACTCCTCCGGCTTTCGGCGGAGGGACCAAGGTGGAGATCAGACGAACTGTGGCTGCACCAT32990.3747011420491007No Hit
CTCTAACACTCTCCCCTGTTGAAGCTCTTTGTGACGGGCGAGCTCAGGCC31730.36039003447159645No Hit
GGCGTAGACTTTGTGTTTCTCGTAGTCTGCTTTGCTCAGCGTCAGGGTGC26290.29860239540681593No Hit
GGGGGGGGGGGGGGGGGGGGGGGGGGGGGGGGGGGGGGGGGGGGGGGGGG25900.29417276687092175No Hit
CTCCCAGGAGAGTGTCACAGAGCAGGACAGCAAGGACAGCACCTACAGCC25590.2906517800859802No Hit
CTATAATATTGCTGACAGTAATAAACCGCCACATCCTCAGCCTGCAGGCTGCTGATGCTGAGAGTGAAATCTGTC24810.28179252301419183No Hit
CTTTTATATAGTTCCAACAACAAGAACTACTTAGCTTGGTACCAGCAGAAAGTTGGACAGCCTCCTAAGTTGCT23590.2679357363121639No Hit
GGCTGGAACTGAGGAGCAGGTGGGGGCACTTCTCCCTCTAACACTCTCCC22980.26100734296114986No Hit
TTATAGTACTCCTCCGGCTTTCGGCGGAGGGACCAAGGTGGAGATCAGACGAACTGTGGCTGCACCATCTGTCTT22670.25748635617620835No Hit
GTCCTGCTCTGTGACACTCTCCTGGGAGTTACCCGATTGGAGGGCGTTATCCACCTTCCACTGTACTTTGGCCT22150.2515801847950161No Hit
GTCTACGCCTGCGAAGTCACCCATCAGGGCCTGAGCTCGCCCGTCACAAA21570.24499253210060937No Hit
CAACAGAGGCAGTTCCAGATTTCAACTGCTCATCAGATGGCGGGAAGATG20270.23022710364762872No Hit
GTACTATAATATTGCTGACAGTAATAAACCGCCACATCCTCAGCCTGCAGGCTGCTGATGCTGAGAGTGAAAT19790.2247752531419128No Hit
GTACAGTGGAAGGTGGATAACGCCCTCCAATCGGGTAACTCCCAGGAGAG19770.22454809270417464No Hit
CTCCCTGACTGTGTCTCTGGGCGAGAGGGCCACCATCAACTGCAAGTCAAGCCAGCGTCTTTTATATAGTTCCAA19310.21932340263619687No Hit
GCGTCAGGGTGCTGCTGAGGCTGTAGGTGCTGTCCTTGCTGTCCTGCTCT19180.21784685979089882No Hit
GGATGGGAGGGGGTCAGGCTGGAACTGAGGAGCAGGTGGGGGCACTTCTCCCTCTAACACTCTCCCCTGTTGAAG18690.21228142906631384No Hit
GTCTTCATCTTCCCGCCATCTGATGAGCAGTTGAAATCTGGAACTGCCTCTGTTGTGTGCCTGCTGAATAACTT18050.20501229505869256No Hit
CAATAGGGGTAGGTCCCCTGTGGAAAAAGGGTCAGAGGCCAAAGGATGGGAGGGGGTCAGGCTGGAACTGAGGAG17840.20262711046244186No Hit
GTGTTGCAGACCCAGGTCTTCATTTCTCTGTTGCTCTGGATCTCTGGTGG17830.2025135302435728No Hit
GTTTATTACTGTCAGCAATATTATAGTACTCCTCCGGCTTTCGGCGGAGGGACCAAGGTGGAGATCAGACGAA16940.1924048907642245No Hit
GTAATAAACCGCCACATCCTCAGCCTGCAGGCTGCTGATGCTGAGAGTGAAATCTGTCCCAGACCCGCTGGCA16880.19172340945101002No Hit
GAGCAGGACAGCAAGGACAGCACCTACAGCCTCAGCAGCACCCTGACGCT16490.18729378091511584No Hit
CCACTGTACTTTGGCCTCTCTGGGATAGAAGTTATTCAGCAGGCACACAACAGAGGCAGTTCCAGATTTCAACTG16400.1862715589452941No Hit
CTCTGGGATAGAAGTTATTCAGCAGGCACACAACAGAGGCAGTTCCAGAT16100.18286415237922163No Hit
ACTTAGGAGGCTGTCCAACTTTCTGCTGGTACCAAGCTAAGTAGTTCTTGTTGTTGGAACTATATAAAAGACG15980.18150118975279267No Hit
GGCTGTAGGTGCTGTCCTTGCTGTCCTGCTCTGTGACACTCTCCTGGGAGTTACCCGATTGGAGGGCGTTATCCA15560.17673082056029124No Hit
GTCTCCAGACTCCCTGACTGTGTCTCTGGGCGAGAGGGCCACCATCAACT15420.17514069749612407No Hit
GTGGCTGCACCATCTGTCTTCATCTTCCCGCCATCTGATGAGCAGTTGAAATCTGGAACTGCCTCTGTTGTGTGC15330.17411847552630233No Hit
CTCCTAAGTTGCTCTTTTACTGGGCATCTATTCGGGAATCCGGGGTCCCT15140.17196045136778979No Hit
CCCTGACTGTGTCTCTGGGCGAGAGGGCCACCATCAACTGCAAGTCAAGC14670.16662218108094295No Hit
GTCCCTGACCGATTCAGTGCCAGCGGGTCTGGGACAGATTTCACTCTCAG14500.16469131736016857No Hit
TCCTCAGCCTGCAGGCTGCTGATGCTGAGAGTGAAATCTGTCCCAGACCCGCTGGCACTGAATCGGTCAGGGACC14400.16355551517147773No Hit
CCCTGACGCTGAGCAAAGCAGACTACGAGAAACACAAAGTCTACGCCTGCGAAGTCACCCATCAGGGCCTGAGCT13900.15787650422802363No Hit
GAGTGAAATCTGTCCCAGACCCGCTGGCACTGAATCGGTCAGGGACCCCG13580.15424193722421303No Hit
ATCCCAGAGAGGCCAAAGTACAGTGGAAGGTGGATAACGCCCTCCAATCG13540.1537876163487367No Hit
GGCTGAGGATGTGGCGGTTTATTACTGTCAGCAATATTATAGTACTCCTC12930.14685922299772272No Hit
ATATAAAAGACGCTGGCTTGACTTGCAGTTGATGGTGGCCCTCTCGCCCAGAGACACAGTCAGGGAGTCTGGAGA12830.1457234208090319No Hit
GTGCAGCCACAGTTCGTCTGATCTCCACCTTGGTCCCTCCGCCGAAAGCC12510.14208885380522127No Hit
GTTGTGTGCCTGCTGAATAACTTCTATCCCAGAGAGGCCAAAGTACAGTG12490.1418616933674831No Hit
ATTCAGTGCCAGCGGGTCTGGGACAGATTTCACTCTCAGCATCAGCAGCC12490.1418616933674831No Hit
GTCCCAGACCCGCTGGCACTGAATCGGTCAGGGACCCCGGATTCCCGAAT12400.14083947139766137No Hit
GCTTTGCTCAGCGTCAGGGTGCTGCTGAGGCTGTAGGTGCTGTCCTTGCT12280.1394765087712324No Hit
CCTACAGCCTCAGCAGCACCCTGACGCTGAGCAAAGCAGACTACGAGAAACACAAAGTCTACGCCTGCGAAGTCA11960.1358419417674218No Hit
GGCCAAAGGATGGGAGGGGGTCAGGCTGGAACTGAGGAGCAGGTGGGGGCACTTCTCCCTCTAACACTCTCCC11910.13527404067307638No Hit
GGCCCTGATGGGTGACTTCGCAGGCGTAGACTTTGTGTTTCTCGTAGTCTGCTTTGCTCAGCGTCAGGGTGCTG11690.1327752758579566No Hit
GACCGATTCAGTGCCAGCGGGTCTGGGACAGATTTCACTCTCAGCATCAG11650.13232095498248025No Hit
GTACTTTGGCCTCTCTGGGATAGAAGTTATTCAGCAGGCACACAACAGAGGCAGTTCCAGATTTCAACTGCTCAT11620.131980214325873No Hit
GTCCTTGCTGTCCTGCTCTGTGACACTCTCCTGGGAGTTACCCGATTGGAGGGCGTTATCCACCTTCCACTGTA11370.12914070885414597No Hit
GATTTCACTCTCAGCATCAGCAGCCTGCAGGCTGAGGATGTGGCGGTTTATTACTGTCAGCAATATTATAGTACT11320.12857280775980057No Hit
GTTGAAATCTGGAACTGCCTCTGTTGTGTGCCTGCTGAATAACTTCTATC11090.12596046272581166No Hit
GGCAGGGGCAGCAAGATGGTGTTGCAGACCCAGGTCTTCATTTCTCTGTT10800.1226666363786083No Hit
GAGCAAAGCAGACTACGAGAAACACAAAGTCTACGCCTGCGAAGTCACCC10720.12175799462765566No Hit
GTGTCACAGAGCAGGACAGCAAGGACAGCACCTACAGCCTCAGCAGCACC10670.12119009353331023No Hit
GTCAGGGACCCCGGATTCCCGAATAGATGCCCAGTAAAAGAGCAACTTAGGAGGCTGTCCAACTTTCTGCTGGTA10310.1171012056540233No Hit
GTAATATACGGCTGTGTCCTCGATTTTCAGGCTGTTCATCTGCAGATACA10290.11687404521628514No Hit
CCTCTGTTGTGTGCCTGCTGAATAACTTCTATCCCAGAGAGGCCAAAGTA10240.11630614412193971No Hit
GAATCGGTCAGGGACCCCGGATTCCCGAATAGATGCCCAGTAAAAGAGCAACTTAGGAGGCTGTCCAACTTTCTG10230.11619256390307064No Hit
CTACTTAGCTTGGTACCAGCAGAAAGTTGGACAGCCTCCTAAGTTGCTCTTTTACTGGGCATCTATTCGGGAATC10230.11619256390307064No Hit
AACTGTGGCTGCACCATCTGTCTTCATCTTCCCGCCATCTGATGAGCAGT10220.11607898368420155No Hit
GTGGAAGGTGGATAACGCCCTCCAATCGGGTAACTCCCAGGAGAGTGTCACAGAGCAGGACAGCAAGGACAGCAC10180.11562466280872523No Hit
ACCTTCCACTGTACTTTGGCCTCTCTGGGATAGAAGTTATTCAGCAGGCA10130.11505676171437983No Hit
CTTCTATCCCAGAGAGGCCAAAGTACAGTGGAAGGTGGATAACGCCCTCCAATCGGGTAACTCCCAGGAGAGTGT10100.11471602105777258No Hit
CAGTAATAAACCGCCACATCCTCAGCCTGCAGGCTGCTGATGCTGAGAGTGAAATCTGTCCCAGACCCGCTGGCA10090.11460244083890349No Hit
GTGCCAGCGGGTCTGGGACAGATTTCACTCTCAGCATCAGCAGCCTGCAGGCTGAGGATGTGGCGGTTTATTA9900.11244441668039094No Hit
GTTGAAGCTCTTTGTGACGGGCGAGCTCAGGCCCTGATGGGTGACTTCGC9760.11085429361622379No Hit
GTGGAAAAAGGGTCAGAGGCCAAAGGATGGGAGGGGGTCAGGCTGGAACTGAGGAGCAGGTGGGGGCACTTCTCC9760.11085429361622379No Hit
GCCTCTGTTGTGTGCCTGCTGAATAACTTCTATCCCAGAGAGGCCAAAGTACAGTGGAAGGTGGATAACGCCCTC9620.10926417055205666No Hit
GTGTGACCTGGAGCGAAAGCGGACAGAACGTGACCGCCAGAAACTTCCCACCTAGCCAGGATGCCTCCGGGGAC9610.10915059033318757No Hit
AAGTTGGACAGCCTCCTAAGTTGCTCTTTTACTGGGCATCTATTCGGGAATCCGGGGTCCCTGACCGATTCAGTG9520.10812836836336583No Hit
GCCTCCTAAGTTGCTCTTTTACTGGGCATCTATTCGGGAATCCGGGGTCCCTGACCGATTCAGTGCCAGCGGGT9370.1064246650803296No Hit
GTAAAAGAGCAACTTAGGAGGCTGTCCAACTTTCTGCTGGTACCAAGCTA9350.10619750464259145No Hit
GGATCTCTGGTGGCTACGGGGACATCGTGATGACCCAGTCTCCAGACTCCCTGACTGTGTCTCTGGGCGAGAGGG9320.1058567639859842No Hit
GTTCAAGGACCACCTGAGCGTGACCTCTGTGGCTGCTACAGCGTGTCCAG9230.10483454201616246No Hit
GTGCTGTCCTTGCTGTCCTGCTCTGTGACACTCTCCTGGGAGTTACCCGATTGGAGGGCGTTATCCACCTTCCA9080.10313083873312624No Hit
CCCCTGTTGAAGCTCTTTGTGACGGGCGAGCTCAGGCCCTGATGGGTGACTTCGCAGGCGTAGACTTTGTGTTT9060.10290367829538806No Hit
GAGTAATAGTGATGGTGGTGCAACAGACTACGCTACTGCCCTTGAAGGCA8980.10199503654443542No Hit
GCACACAACAGAGGCAGTTCCAGATTTCAACTGCTCATCAGATGGCGGGA8880.10085923435574459No Hit
CTACGGGGACATCGTGATGACCCAGTCTCCAGACTCCCTGACTGTGTCTCTGGGCGAGAGGGCCACCATCAACTG8850.10051849369913735No Hit

[OK]Adapter Content

Adapter graph

[WARN]Kmer Content

Kmer graph

SequenceCountPValueObs/Exp MaxMax Obs/Exp Position
GTCCGTA200.00799328650.67800512
GTATCAA15700.038.9743271
GGTATCA5950.038.635961
TATTAGA601.5502228E-533.78152
TCAACGC18400.033.410514
TCTTGAG6250.033.4049870
ATCAACG18350.033.3155823
CAACGCA18650.033.1437645
TATCAAC18500.033.051092
AACGCAG18850.032.797696
ACGCAGA19400.031.8678637
CGCAGAG19600.031.3703168
ATTAGAG652.6830388E-531.177613
ACAGGTA1101.3151293E-930.733151
GTGGTAT2200.030.733151
CAGAGTA19900.030.55786310
AGAGTAC19850.030.46637311
GCAGAGT20150.030.3463959
GTGACGA450.00509054330.06728220
AGGTACG450.00512319130.0282
Produced by FastQC (version 0.11.5)