FastQCFastQC Report
Sat 23 Apr 2022
EGAF00004684444

Summary

[OK]Basic Statistics

MeasureValue
FilenameEGAF00004684444
File typeConventional base calls
EncodingSanger / Illumina 1.9
Total Sequences799271
Sequences flagged as poor quality0
Sequence length20-76
%GC56

[OK]Per base sequence quality

Per base quality graph

[OK]Per tile sequence quality

Per base quality graph

[OK]Per sequence quality scores

Per Sequence quality graph

[FAIL]Per base sequence content

Per base sequence content

[FAIL]Per sequence GC content

Per sequence GC content graph

[OK]Per base N content

N content graph

[WARN]Sequence Length Distribution

Sequence length distribution

[FAIL]Sequence Duplication Levels

Duplication level graph

[WARN]Overrepresented sequences

SequenceCountPercentagePossible Source
GCCCAAGGCTGCCCCCTCGGTCACTCTGTTCCCGCCCTCCTCTGAGGAGC36550.45729170706806577No Hit
GTTCTGGGGTGTCTCCACCATGGCCTGGACCCCTCTCTGGCTCACTCTCC34930.43702323742510363No Hit
GTAATATACAGCCGTGTCTGCGGCGGTCACAGAGCTCAGCCTCAGGGAGA30630.3832242130641547No Hit
GCCTTGGGCTGACCTAGGACGGTCAGCTTGGTCCCTCCGCCGAAGACCTG29780.37258952220210667No Hit
CCTCTGAGGAGCTTCAAGCCAACAAGGCCACACTGGTGTGTCTCATAAGT24470.30615398281684186No Hit
GTGTGGTGGTCTCCACTCCCGCCTTGACGGGGCTGCTATCTGCCTTCCAG24300.30402704464443225No Hit
GACCTAGGACGGTCAGCTTGGTCCCTCCGCCGAAGACCTGATGCTTACCA22620.2830078909406196No Hit
CTATTACTGTAACTCCCGGGACAGCAGTGGTAAGCATCAGGTCTTCGGCG22120.27675219043353255No Hit
CCTCAGAGGAGGGCGGGAACAGAGTGACCGAGGGGGCAGCCTTGGGCTGACCTAGGACGGTCAGCTTGGTCCCTC20560.25723440485142085No Hit
GTGCAGGGAGAAGGGCTTGATGCCTTGGGGTGGGAGGAGAGACCCCTCCCCTGGGATCCTGCAGCTCTAGTCTCC19780.24747551206036503No Hit
CCTATGAACATTCTGTAGGGGCCACTGTCTTCTCCACGGTGCTCCCTTCATGCGTGACCTGGCAGCTGTAGCTT19060.2384673033301596No Hit
TCCTAGGTCAGCCCAAGGCTGCCCCCTCGGTCACTCTGTTCCCGCCCTCCTCTGAGGAGCTTCAAGCCAACAAGG18790.2350892250563326No Hit
GTATATTACTGTGCGAGACAGTTGGCCTATTACTATGATACTAGTGGTCCCCAGGAGGCTTTTGATATTTGGGGC18030.22558056028556023No Hit
GTCTTCTCCACGGTGCTCCCTTCATGCGTGACCTGGCAGCTGTAGCTTCT17540.2194499737886149No Hit
GGGTTTATTGAGTGCAGGGAGAAGGGCTTGATGCCTTGGGGTGGGAGGAGAGACCCCTCCCCTGGGATCCTGCAG17320.21669746556549654No Hit
CAGTAATAGTCACCCTCATCTTCCGCCTGAGCCCCAGTGATGGTCAAGGA17060.21344450130181125No Hit
GTCCTAGGTCAGCCCAAGGCTGCCCCCTCGGTCACTCTGTTCCCGCCCTC16650.2083148268859999No Hit
CTCTGTTCCCGCCCTCCTCTGAGGAGCTTCAAGCCAACAAGGCCACACTGGTGTGTCTCATAAGTGACTTCTACC16350.20456140658174762No Hit
CTCTTTGCATAGGTTCTGTGGTTTCTTCTGAGCTGACTCAGGACCCTGCT16070.20105821429777884No Hit
CTCCTCACTCTTTGCATAGGTTCTGTGGTTTCTTCTGAGCTGACTCAGGACCCTGCTGTGTCTGTGGCCTTGGGA15410.1928006896284239No Hit
GTAGAGGGGCCTGTCCTGGCTTCTGGTGGTACCAGCTTGCATAATAGGTT15360.1921751195777152No Hit
GTTTGGAGGGTGTGGTGGTCTCCACTCCCGCCTTGACGGGGCTGCTATCTGCCTTCCAGGCCACTGTCACGGCTC15300.19142443551686475No Hit
GTGCTCCCTTCATGCGTGACCTGGCAGCTGTAGCTTCTGTGGGACTTCCACTGCTCAGGCGTCAGGCTCAGATAG15250.19079886546615604No Hit
GTCCTGGCTTCTGGTGGTACCAGCTTGCATAATAGGTTTTGAGGCTGTCTCCTTGGCATGTGATCCTGACTGT15090.18879704130388816No Hit
ACTCAGGACCCTGCTGTGTCTGTGGCCTTGGGACAGACAGTCAGGATCACATGCCAAGGAGACAGCCTCAAAAC14960.1871705591720455No Hit
AGCCAGGACAGGCCCCTCTACTTGTCATCTATGGTAAAAACAACCGGCCCTCAGGGATCCCAGACCGATTCTCTG14540.18191577074609236No Hit
GGTTTATTGAGTGCAGGGAGAAGGGCTTGATGCCTTGGGGTGGGAGGAGAGACCCCTCCCCTGGGATCCTGCAG14490.18129020069538368No Hit
GCTCAGGCGTCAGGCTCAGATAGCTGCTGGCCGCGTACTTGTTGTTGCTTTGTTTGGAGGGTGTGGTGGTCTCCA14330.1792883765331158No Hit
GAGAAGGGCTTGATGCCTTGGGGTGGGAGGAGAGACCCCTCCCCTGGGAT14220.17791212242155666No Hit
CTTCATGCGTGACCTGGCAGCTGTAGCTTCTGTGGGACTTCCACTGCTCAGGCGTCAGGCTCAGATAGCTGCTGG13910.17403358810716266No Hit
CACCAGTGTGGCCTTGTTGGCTTGAAGCTCCTCAGAGGAGGGCGGGAACAGAGTGACCGAGGGGGCAGCCTTGGG13700.17140619389418607No Hit
GGCTCAGATAGCTGCTGGCCGCGTACTTGTTGTTGCTTTGTTTGGAGGGTGTGGTGGTCTCCACTCCCGCCTTGA13330.16677697551894163No Hit
CCCTTCATGCGTGACCTGGCAGCTGTAGCTTCTGTGGGACTTCCACTGCTCAGGCGTCAGGCTCAGATAGCTG13220.16540072140738246No Hit
CCCCTCTACTTGTCATCTATGGTAAAAACAACCGGCCCTCAGGGATCCCA13150.1645249233363903No Hit
GGCCTGGACCCCTCTCTGGCTCACTCTCCTCACTCTTTGCATAGGTTCTGTGGTTTCTTCTGAGCTGACTCAGGA12950.16202264313355544No Hit
GTCTGTGGCCTTGGGACAGACAGTCAGGATCACATGCCAAGGAGACAGCCTCAAAACCTATTATGCAAGCTGGTA12610.15776876678873622No Hit
GTCTCTGGTGGCTCCATCAGTAGTAGTAGTTACTCCTGGGGCTGGATCCG12340.1543906885149092No Hit
CTTATGAGACACACCAGTGTGGCCTTGTTGGCTTGAAGCTCCTCAGAGGA12260.15338977643377527No Hit
CCACCACACCCTCCAAACAAAGCAACAACAAGTACGCGGCCAGCAGCTAT12220.15288932039320832No Hit
GCTCTAGTCTCCCGTGGTGGGGGTGAGGGTTGAGAACCTATGAACATTCT12220.15288932039320832No Hit
GCCTTGGGGTGGGAGGAGAGACCCCTCCCCTGGGATCCTGCAGCTCTAGT12160.15213863633235786No Hit
GTTCCCGCCCTCCTCTGAGGAGCTTCAAGCCAACAAGGCCACACTGGTGT11830.14800987399768037No Hit
ACACTGGTGTGTCTCATAAGTGACTTCTACCCGGGAGCCGTGACAGTGGC11820.14788475998753864No Hit
CTTATACACATCTCCGAGCCCACGAGACCGTACTAGATCTCGTATGCCGT11550.14450668171371162No Hit
GCTATCTGCCTTCCAGGCCACTGTCACGGCTCCCGGGTAGAAGTCACTTA10940.13687472709506537No Hit
GGACAGGCCCCTCTACTTGTCATCTATGGTAAAAACAACCGGCCCTCAGG10620.13287107877052964No Hit
TCACTGGGGCTCAGGCGGAAGATGAGGGTGACTATTACTGTAACTCCCGG10390.1299934565372696No Hit
GGTGTGTCTCATAAGTGACTTCTACCCGGGAGCCGTGACAGTGGCCTGGA10290.12874231643585218No Hit
CCCTCATCTTCCGCCTGAGCCCCAGTGATGGTCAAGGAAGCTGTGTTTCCTGAGATGGAGGCAGAGAATCGGT10250.1282418603952852No Hit
ACCTAGGACGGTCAGCTTGGTCCCTCCGCCGAAGACCTGATGCTTACCAC10190.12749117633443474No Hit
CCCCTGGGATCCTGCAGCTCTAGTCTCCCGTGGTGGGGGTGAGGGTTGAG10150.1269907202938678No Hit
GGAACAGAGTGACCGAGGGGGCAGCCTTGGGCTGACCTAGGACGGTCAGC10090.12624003623301733No Hit
GCAGTGGTAAGCATCAGGTCTTCGGCGGAGGGACCAAGCTGACCGTCCTA9670.12098524780706418No Hit
ATCTGAGCCTGACGCCTGAGCAGTGGAAGTCCCACAGAAGCTACAGCTGC9520.11910853765493806No Hit
TCCTTGGGGCTGAAGCCACGTGCCAGGCACGTCAGCGTCACCAGCTCGTT9510.11898342364479633No Hit
GGCTCACTCTCCTCACTCTTTGCATAGGTTCTGTGGTTTCTTCTGAGCTGACTCAGGACCCTGCTGTGTCTGTGG9510.11898342364479633No Hit
GAGTGACCGAGGGGGCAGCCTTGGGCTGACCTAGGACGGTCAGCTTGGTCCCTCCGCCGAAGACCTGATGCTTAC9370.11723182750281193No Hit
GTACGGGAGCTGTGGGCTCAGAAGCAGAGTTCTGGGGTGTCTCCACCATGGCCTGGACCCCTCTCTGGCTCACT9360.1171067134926702No Hit
GTCACATCCTGGCTGGGATTCGTGTAGTGCTTCACGTGGCATGTCACGGA9350.11698159948252845No Hit
CTGTAGGGGCCACTGTCTTCTCCACGGTGCTCCCTTCATGCGTGACCTGG9260.11585557339125278No Hit
GTATGCTGGTCACAGCGAAGGTGGTGGTGCCCTGGCTGGGCTCCTGCCGG9230.11548023136082755No Hit
GGGCTGCTATCTGCCTTCCAGGCCACTGTCACGGCTCCCGGGTAGAAGTCACTTATGAGACACACCAGTGTGGC9200.11510488933040232No Hit
AGCTGGTACCACCAGAAGCCAGGACAGGCCCCTCTACTTGTCATCTATGGTAAAAACAACCGGCCCTCAGGGATC9170.11472954729997711No Hit
GCTGTAGCTTCTGTGGGACTTCCACTGCTCAGGCGTCAGGCTCAGATAGC9130.11422909125941014No Hit
CCCGTGGTGGGGGTGAGGGTTGAGAACCTATGAACATTCTGTAGGGGCCA9090.11372863521884317No Hit
GTTGAGAACCTATGAACATTCTGTAGGGGCCACTGTCTTCTCCACGGTGCTCCCTTCATGCGTGACCTGGCAG9080.11360352120870143No Hit
CCCCTGCACTGTCTCTGGTGGCTCCATCAGTAGTAGTAGTTACTCCTGGG9010.11272772313770923No Hit
GTCTCCACCATGGCCTGGACCCCTCTCTGGCTCACTCTCCTCACTCTTTGCATAGGTTCTGTGGTTTCTTCTGAG8990.11247749511742575No Hit
GCTTGGTCCCTCCGCCGAAGACCTGATGCTTACCACTGCTGTCCCGGGAGTTACAGTAATAGTCACCCTCATCTT8960.11210215308700053No Hit
CTTCTCCACGGTGCTCCCTTCATGCGTGACCTGGCAGCTGTAGCTTCTGT8750.10947475887402396No Hit
TACCACTGCTGTCCCGGGAGTTACAGTAATAGTCACCCTCATCTTCCGCCTGAGCCCCAGTGATGGTCAAGGAAG8610.10772316273203957No Hit
GTTCAAGGACCACCTGAGCGTGACCTCTGTGGCTGCTACAGCGTGTCCAG8590.10747293471175608No Hit
GTATCAACGCAGAGTACGGGAGCTGTGGGCTCAGAAGCAGAGTTCTGGGGTGTCTCCACCATGGCCTGGACCCCT8590.10747293471175608No Hit
GTAATAGTCACCCTCATCTTCCGCCTGAGCCCCAGTGATGGTCAAGGAAG8150.10196791826551944No Hit
GACTTCCACTGCTCAGGCGTCAGGCTCAGATAGCTGCTGGCCGCGTACTT8140.10184280425537771No Hit
GTGCAGCGACAGTCGGGGGTGGCAGCATGGGGGAGGTGGGGGAACTGGGC8130.10171769024523597No Hit
CTCTACTTGTCATCTATGGTAAAAACAACCGGCCCTCAGGGATCCCAGAC8090.101217234204669No Hit
GCTCAGTAGCAGGTGCCGTCCACCTCCGCCATGACAACAGACACATTGAC8080.10109212019452726No Hit

[OK]Adapter Content

Adapter graph

[FAIL]Kmer Content

Kmer graph

SequenceCountPValueObs/Exp MaxMax Obs/Exp Position
AGGGGGG2450.064.42271470
TACGGAT8200.062.5568270
TACGTAT350.00524475656.3698870
CGTATGC1950.054.9462841
TCGTATG1950.054.8696440
CGTACTA1950.054.26754429
TCTCGTA1900.052.55050338
GGTATCA6550.052.5285531
GACCGTA2050.051.5653826
ATCTCGT1950.051.13191637
ATAACGC200.00774392151.0855333
TACTAGA2100.050.45139731
ACCGTAC2100.050.35655627
GTATGCC2250.049.22812742
ATGCCGT2200.048.89795744
GTACTAG2100.048.7841230
ACTAGAT2200.048.1914332
CTAGATC2100.047.2587733
TTATACA2200.046.441392
TAGATCT2150.046.20329334
Produced by FastQC (version 0.11.5)