FastQCFastQC Report
Sat 23 Apr 2022
EGAF00004684445

Summary

[OK]Basic Statistics

MeasureValue
FilenameEGAF00004684445
File typeConventional base calls
EncodingSanger / Illumina 1.9
Total Sequences799271
Sequences flagged as poor quality0
Sequence length20-76
%GC56

[OK]Per base sequence quality

Per base quality graph

[OK]Per tile sequence quality

Per base quality graph

[OK]Per sequence quality scores

Per Sequence quality graph

[FAIL]Per base sequence content

Per base sequence content

[FAIL]Per sequence GC content

Per sequence GC content graph

[OK]Per base N content

N content graph

[WARN]Sequence Length Distribution

Sequence length distribution

[FAIL]Sequence Duplication Levels

Duplication level graph

[WARN]Overrepresented sequences

SequenceCountPercentagePossible Source
GCCCAAGGCTGCCCCCTCGGTCACTCTGTTCCCGCCCTCCTCTGAGGAGC35550.4447803060538916No Hit
GTTCTGGGGTGTCTCCACCATGGCCTGGACCCCTCTCTGGCTCACTCTCC33200.4153785136705823No Hit
GTAATATACAGCCGTGTCTGCGGCGGTCACAGAGCTCAGCCTCAGGGAGA29960.374841574384658No Hit
GCCTTGGGCTGACCTAGGACGGTCAGCTTGGTCCCTCCGCCGAAGACCTG27200.3403101075855373No Hit
GGGGGGGGGGGGGGGGGGGGGGGGGGGGGGGGGGGGGGGGGGGGGGGGGG24360.3047777287052827No Hit
GTGTGGTGGTCTCCACTCCCGCCTTGACGGGGCTGCTATCTGCCTTCCAG24220.3030261325632983No Hit
CCTCTGAGGAGCTTCAAGCCAACAAGGCCACACTGGTGTGTCTCATAAGT23410.29289189774181723No Hit
CTATTACTGTAACTCCCGGGACAGCAGTGGTAAGCATCAGGTCTTCGGCG22750.28463437307246225No Hit
GACCTAGGACGGTCAGCTTGGTCCCTCCGCCGAAGACCTGATGCTTACCA19840.24822619612121544No Hit
CCTCAGAGGAGGGCGGGAACAGAGTGACCGAGGGGGCAGCCTTGGGCTGACCTAGGACGGTCAGCTTGGTCCCTC18550.23208648881293076No Hit
GGGTTTATTGAGTGCAGGGAGAAGGGCTTGATGCCTTGGGGTGGGAGGAGAGACCCCTCCCCTGGGATCCTGCAG18360.2297093226202377No Hit
GTGCAGGGAGAAGGGCTTGATGCCTTGGGGTGGGAGGAGAGACCCCTCCCCTGGGATCCTGCAGCTCTAGTCTCC18260.22845818251882027No Hit
TCCTAGGTCAGCCCAAGGCTGCCCCCTCGGTCACTCTGTTCCCGCCCTCCTCTGAGGAGCTTCAAGCCAACAAGG17890.22382896414357584No Hit
GTCTTCTCCACGGTGCTCCCTTCATGCGTGACCTGGCAGCTGTAGCTTCT17460.21844906170748096No Hit
CCTATGAACATTCTGTAGGGGCCACTGTCTTCTCCACGGTGCTCCCTTCATGCGTGACCTGGCAGCTGTAGCTT17190.21507098343365394No Hit
CTCCTCACTCTTTGCATAGGTTCTGTGGTTTCTTCTGAGCTGACTCAGGACCCTGCTGTGTCTGTGGCCTTGGGA16540.20693857277444072No Hit
GTATATTACTGTGCGAGACAGTTGGCCTATTACTATGATACTAGTGGTCCCCAGGAGGCTTTTGATATTTGGGGC16400.20518697663245633No Hit
GTCCTAGGTCAGCCCAAGGCTGCCCCCTCGGTCACTCTGTTCCCGCCCTC15990.20005730221664492No Hit
GTGCTCCCTTCATGCGTGACCTGGCAGCTGTAGCTTCTGTGGGACTTCCACTGCTCAGGCGTCAGGCTCAGATAG15830.19805547805437704No Hit
CTCTGTTCCCGCCCTCCTCTGAGGAGCTTCAAGCCAACAAGGCCACACTGGTGTGTCTCATAAGTGACTTCTACC15610.19530296983125875No Hit
CAGTAATAGTCACCCTCATCTTCCGCCTGAGCCCCAGTGATGGTCAAGGA15470.19355137368927436No Hit
AGCCAGGACAGGCCCCTCTACTTGTCATCTATGGTAAAAACAACCGGCCCTCAGGGATCCCAGACCGATTCTCTG15050.1882965852633212No Hit
CTCTTTGCATAGGTTCTGTGGTTTCTTCTGAGCTGACTCAGGACCCTGCT15040.18817147125317946No Hit
ACTCAGGACCCTGCTGTGTCTGTGGCCTTGGGACAGACAGTCAGGATCACATGCCAAGGAGACAGCCTCAAAAC14770.18479339297935243No Hit
GTTTGGAGGGTGTGGTGGTCTCCACTCCCGCCTTGACGGGGCTGCTATCTGCCTTCCAGGCCACTGTCACGGCTC14590.1825413407968011No Hit
GGTTTATTGAGTGCAGGGAGAAGGGCTTGATGCCTTGGGGTGGGAGGAGAGACCCCTCCCCTGGGATCCTGCAG14180.17741166638098968No Hit
GTCCTGGCTTCTGGTGGTACCAGCTTGCATAATAGGTTTTGAGGCTGTCTCCTTGGCATGTGATCCTGACTGT13580.16990482577248517No Hit
GTAGAGGGGCCTGTCCTGGCTTCTGGTGGTACCAGCTTGCATAATAGGTT13520.16915414171163473No Hit
GCTCAGGCGTCAGGCTCAGATAGCTGCTGGCCGCGTACTTGTTGTTGCTTTGTTTGGAGGGTGTGGTGGTCTCCA13410.16777788760007556No Hit
GAGAAGGGCTTGATGCCTTGGGGTGGGAGGAGAGACCCCTCCCCTGGGAT13120.16414958130596508No Hit
GTCTGTGGCCTTGGGACAGACAGTCAGGATCACATGCCAAGGAGACAGCCTCAAAACCTATTATGCAAGCTGGTA12630.1580189948090197No Hit
CTTCATGCGTGACCTGGCAGCTGTAGCTTCTGTGGGACTTCCACTGCTCAGGCGTCAGGCTCAGATAGCTGCTGG12510.15651762668731883No Hit
GGCTCAGATAGCTGCTGGCCGCGTACTTGTTGTTGCTTTGTTTGGAGGGTGTGGTGGTCTCCACTCCCGCCTTGA12470.15601717064675186No Hit
CCCCTCTACTTGTCATCTATGGTAAAAACAACCGGCCCTCAGGGATCCCA12460.1558920566366101No Hit
CCACCACACCCTCCAAACAAAGCAACAACAAGTACGCGGCCAGCAGCTAT12400.15514137257575966No Hit
CACCAGTGTGGCCTTGTTGGCTTGAAGCTCCTCAGAGGAGGGCGGGAACAGAGTGACCGAGGGGGCAGCCTTGGG12370.15476603054533444No Hit
GTTCCCGCCCTCCTCTGAGGAGCTTCAAGCCAACAAGGCCACACTGGTGT12160.15213863633235786No Hit
GCCTTGGGGTGGGAGGAGAGACCCCTCCCCTGGGATCCTGCAGCTCTAGT12070.1510126102410822No Hit
CCCTTCATGCGTGACCTGGCAGCTGTAGCTTCTGTGGGACTTCCACTGCTCAGGCGTCAGGCTCAGATAGCTG11950.1495112421193813No Hit
GGCCTGGACCCCTCTCTGGCTCACTCTCCTCACTCTTTGCATAGGTTCTGTGGTTTCTTCTGAGCTGACTCAGGA11780.1473843039469717No Hit
CTTATGAGACACACCAGTGTGGCCTTGTTGGCTTGAAGCTCCTCAGAGGA11620.1453824797847038No Hit
GTCTCTGGTGGCTCCATCAGTAGTAGTAGTTACTCCTGGGGCTGGATCCG11610.14525736577456208No Hit
ACACTGGTGTGTCTCATAAGTGACTTCTACCCGGGAGCCGTGACAGTGGC11270.14100348942974286No Hit
GCTCTAGTCTCCCGTGGTGGGGGTGAGGGTTGAGAACCTATGAACATTCT10680.1336217628313801No Hit
GGTGTGTCTCATAAGTGACTTCTACCCGGGAGCCGTGACAGTGGCCTGGA10540.13187016668939572No Hit
ATCTGAGCCTGACGCCTGAGCAGTGGAAGTCCCACAGAAGCTACAGCTGC10200.1276162903445765No Hit
TCACTGGGGCTCAGGCGGAAGATGAGGGTGACTATTACTGTAACTCCCGG10100.12636515024315909No Hit
GTACGGGAGCTGTGGGCTCAGAAGCAGAGTTCTGGGGTGTCTCCACCATGGCCTGGACCCCTCTCTGGCTCACT10050.12573958019245038No Hit
GGACAGGCCCCTCTACTTGTCATCTATGGTAAAAACAACCGGCCCTCAGG9930.12423821207074948No Hit
CCCTCATCTTCCGCCTGAGCCCCAGTGATGGTCAAGGAAGCTGTGTTTCCTGAGATGGAGGCAGAGAATCGGT9880.12361264202004076No Hit
GCAGTGGTAAGCATCAGGTCTTCGGCGGAGGGACCAAGCTGACCGTCCTA9710.12148570384763115No Hit
GCTGTAGCTTCTGTGGGACTTCCACTGCTCAGGCGTCAGGCTCAGATAGC9680.12111036181720594No Hit
GCTATCTGCCTTCCAGGCCACTGTCACGGCTCCCGGGTAGAAGTCACTTA9440.11810762557380412No Hit
GGCTCACTCTCCTCACTCTTTGCATAGGTTCTGTGGTTTCTTCTGAGCTGACTCAGGACCCTGCTGTGTCTGTGG9340.11685648547238672No Hit
GGAACAGAGTGACCGAGGGGGCAGCCTTGGGCTGACCTAGGACGGTCAGC9250.11573045938111104No Hit
ACCTAGGACGGTCAGCTTGGTCCCTCCGCCGAAGACCTGATGCTTACCAC9130.11422909125941014No Hit
GTATCAACGCAGAGTACGGGAGCTGTGGGCTCAGAAGCAGAGTTCTGGGGTGTCTCCACCATGGCCTGGACCCCT9110.11397886323912666No Hit
GTCTCCACCATGGCCTGGACCCCTCTCTGGCTCACTCTCCTCACTCTTTGCATAGGTTCTGTGGTTTCTTCTGAG9030.11297795115799271No Hit
GTTGAGAACCTATGAACATTCTGTAGGGGCCACTGTCTTCTCCACGGTGCTCCCTTCATGCGTGACCTGGCAG9030.11297795115799271No Hit
TACCACTGCTGTCCCGGGAGTTACAGTAATAGTCACCCTCATCTTCCGCCTGAGCCCCAGTGATGGTCAAGGAAG8750.10947475887402396No Hit
GAGTGACCGAGGGGGCAGCCTTGGGCTGACCTAGGACGGTCAGCTTGGTCCCTCCGCCGAAGACCTGATGCTTAC8750.10947475887402396No Hit
CCCCTGGGATCCTGCAGCTCTAGTCTCCCGTGGTGGGGGTGAGGGTTGAG8720.10909941684359872No Hit
AGCTGGTACCACCAGAAGCCAGGACAGGCCCCTCTACTTGTCATCTATGGTAAAAACAACCGGCCCTCAGGGATC8600.10759804872189783No Hit
GTTCAAGGACCACCTGAGCGTGACCTCTGTGGCTGCTACAGCGTGTCCAG8570.1072227066914726No Hit
GGGCTGCTATCTGCCTTCCAGGCCACTGTCACGGCTCCCGGGTAGAAGTCACTTATGAGACACACCAGTGTGGC8540.10684736466104738No Hit
CCCCTGCACTGTCTCTGGTGGCTCCATCAGTAGTAGTAGTTACTCCTGGG8510.10647202263062215No Hit
CCCGTGGTGGGGGTGAGGGTTGAGAACCTATGAACATTCTGTAGGGGCCA8360.10459531247849602No Hit
GCTTGGTCCCTCCGCCGAAGACCTGATGCTTACCACTGCTGTCCCGGGAGTTACAGTAATAGTCACCCTCATCTT8260.10334417237707862No Hit
CTGTAGGGGCCACTGTCTTCTCCACGGTGCTCCCTTCATGCGTGACCTGG8210.1027186023263699No Hit
CTTCTGAGCTGACTCAGGACCCTGCTGTGTCTGTGGCCTTGGGACAGACA8200.10259348831622817No Hit
GGTTCTGTGGTTTCTTCTGAGCTGACTCAGGACCCTGCTGTGTCTGTGGC8190.10246837430608641No Hit
GTGTGACCTGGAGCGAAAGCGGACAGAACGTGACCGCCAGAAACTTCCCACCTAGCCAGGATGCCTCCGGGGAC8120.10159257623509424No Hit
ACCATGGCCTGGACCCCTCTCTGGCTCACTCTCCTCACTCTTTGCATAGGTTCTGTGGTTTCTTCTGAGCTGACT8110.10146746222495248No Hit
GTCAGGATCACATGCCAAGGAGACAGCCTCAAAACCTATTATGCAAGCTGGTACCACCAGAAGCCAGGACAGGCC8040.1005916641539603No Hit
GTAATAGTCACCCTCATCTTCCGCCTGAGCCCCAGTGATGGTCAAGGAAG8010.10021632212353507No Hit

[OK]Adapter Content

Adapter graph

[WARN]Kmer Content

Kmer graph

SequenceCountPValueObs/Exp MaxMax Obs/Exp Position
CGGTAAT350.00508183456.82104570
TACGGAT7950.056.7019270
CCTAGTA200.00685945452.6766651
GTATCAA16600.040.189531
GGTATCA5850.038.4019281
GTGGTAT2000.037.441881
CTACGGA8300.036.82560769
TGGTATC2050.036.503522
TATTAGA754.551839E-836.2822882
ATCAACG18200.035.87463
TCAACGC18200.035.6877564
TATCAAC18450.035.397352
AACGCAG18750.034.6452456
CAACGCA18750.034.6430785
ACGCAGA19650.033.058447
GACAGGT1650.033.0066151
CGCAGAG19900.032.6431358
TAGGGAT652.0781883E-532.36413650
TCTACGG8650.032.10802568
AGAGTAC20450.031.93151311
Produced by FastQC (version 0.11.5)