FastQCFastQC Report
Wed 27 Apr 2022
EGAF00004684446

Summary

[OK]Basic Statistics

MeasureValue
FilenameEGAF00004684446
File typeConventional base calls
EncodingSanger / Illumina 1.9
Total Sequences913515
Sequences flagged as poor quality0
Sequence length20-76
%GC54

[OK]Per base sequence quality

Per base quality graph

[OK]Per tile sequence quality

Per tile quality graph

[OK]Per sequence quality scores

Per Sequence quality graph

[FAIL]Per base sequence content

Per base sequence content

[FAIL]Per sequence GC content

Per sequence GC content graph

[OK]Per base N content

N content graph

[WARN]Sequence Length Distribution

Sequence length distribution

[FAIL]Sequence Duplication Levels

Duplication level graph

[WARN]Overrepresented sequences

SequenceCountPercentagePossible Source
CTCCTGGGAGTTACCCGATTGGAGGGCGTTATCCACCTTCCACTGTACTTTGGCCTCTCTGGGATAGAAGTTATT30490.33376572907943497No Hit
CTCTAACACTCTCCCCTGTTGAAGCTCTTTGTGACGGGCGAGCTCAGGCC28800.31526575918293626No Hit
GTTAGGACCCAGAGGGAACCATGGAAACCCCAGCGCAGCTTCTCTTCCTCCTGCTACTCTGGCTCCCAGATATTA22960.25133686912639636No Hit
CTCCCAGGAGAGTGTCACAGAGCAGGACAGCAAGGACAGCACCTACAGCC21010.22999075001505176No Hit
GGCGTAGACTTTGTGTTTCTCGTAGTCTGCTTTGCTCAGCGTCAGGGTGC20800.22769193718767616No Hit
GGCTGGAACTGAGGAGCAGGTGGGGGCACTTCTCCCTCTAACACTCTCCC20360.22287537697793686No Hit
CTCCTGCAGGGCCAGCCAGAGTGTTAATAGCAACTACATAGCCTGGTACC19350.2118191819510353No Hit
TATCAATACTGATGGGGGTAGTGCGACGTACGCGGACTCCGTGGAGGGCCGATTCACCGTCTCCAGAGACAACGC17860.19550855760441813No Hit
GTGTTAATAGCAACTACATAGCCTGGTACCAGCAGAAACCTGGCCAGGGTCCCAGGCTCCTCATCTATGGTACAT17650.1932097447770425No Hit
CAATAGGGGTAGGTCCCCTGTGGAAAAAGGGTCAGAGGCCAAAGGATGGGAGGGGGTCAGGCTGGAACTGAGGAG17410.1905825301171847No Hit
GTAATACACAGCCGTGTCCTCGGCTCTCAGACTGTTGATTTGCAGATATA17290.18926892278725582No Hit
CAACAGAGGCAGTTCCAGATTTCAACTGCTCATCAGATGGCGGGAAGATG17120.18740797906985654No Hit
GGATGGGAGGGGGTCAGGCTGGAACTGAGGAGCAGGTGGGGGCACTTCTCCCTCTAACACTCTCCCCTGTTGAAG16600.18171568064016463No Hit
GTCCTGCTCTGTGACACTCTCCTGGGAGTTACCCGATTGGAGGGCGTTATCCACCTTCCACTGTACTTTGGCCT16590.18160621336267058No Hit
CTATTATTGTCAATACTATTATAACTCTCAATTCACTTTCGGCCCTGGGA16230.17766539137288387No Hit
GTCTACGCCTGCGAAGTCACCCATCAGGGCCTGAGCTCGCCCGTCACAAA15650.1713162892782275No Hit
GCGTCAGGGTGCTGCTGAGGCTGTAGGTGCTGTCCTTGCTGTCCTGCTCT15480.16945534556082822No Hit
GTCTGGGACAGACTTCACTCTCACCATCAGTGGACTGGAGCCTGAAGATT15230.16671866362347637No Hit
GTCCCAGACCCACTGCCACTGAACCTGTCTGGGATGCCAGTGGCCCTCTT14810.1621210379687252No Hit
GTACAGTGGAAGGTGGATAACGCCCTCCAATCGGGTAACTCCCAGGAGAG14510.15883701964390295No Hit
GTCCCAGGCTCCTCATCTATGGTACATCCAAGAGGGCCACTGGCATCCCA14230.15577193587406885No Hit
CTATTATAACTCTCAATTCACTTTCGGCCCTGGGACCAAAGTGGATATCAGACGAACTGTGGCTGCACCAT14010.1533636557691992No Hit
GTCTCCAGGCACCCTGTCTTTGTCTCCGGGAGAAACAGGCGCCCTCTCCTGCAGGGCCAGCCAGAGTGTTAATAG13800.1510648429418236No Hit
GTATGCTGGTCACAGCGAAGGTGGTGGTGCCCTGGCTGGGCTCCTGCCGG13740.15040803927685917No Hit
GTCTTTGTCTCCGGGAGAAACAGGCGCCCTCTCCTGCAGGGCCAGCCAGA13650.14942283377941248No Hit
GTCTTCATCTTCCCGCCATCTGATGAGCAGTTGAAATCTGGAACTGCCTCTGTTGTGTGCCTGCTGAATAACTT13600.1488754973919421No Hit
GTGTATTACTGTACAAGAGGGGGTTGGTACATGGACGTCTGGGGCAAGGG13490.1476713573395073No Hit
CTCCAGGCACCCTGTCTTTGTCTCCGGGAGAAACAGGCGCCCTCTCCTGC13330.1459198808996021No Hit
CTCTGGGATAGAAGTTATTCAGCAGGCACACAACAGAGGCAGTTCCAGAT13020.14252639529728575No Hit
CCCTATATCTGCAAATCAACAGTCTGAGAGCCGAGGACACGGCTGTGTAT12610.13803823692002867No Hit
GATCAGGACTGCACACAGAGAACTCACCATGGAGTTTGGGCTGAGCTGGG12420.13595835864764125No Hit
CCACTGTACTTTGGCCTCTCTGGGATAGAAGTTATTCAGCAGGCACACAACAGAGGCAGTTCCAGATTTCAACTG12410.1358488913701472No Hit
GTGCAGCGACAGTCGGGGGTGGCAGCATGGGGGAGGTGGGGGAACTGGGC12410.1358488913701472No Hit
TCCTTGGGGCTGAAGCCACGTGCCAGGCACGTCAGCGTCACCAGCTCGTT12340.13508262042768865No Hit
GTCACATCCTGGCTGGGATTCGTGTAGTGCTTCACGTGGCATGTCACGGA12260.13420688220773605No Hit
GAGTTTATTCAGGGGTGGGGACAGGCGGGTGGCTCAGTAGCAGGTGCCGT12160.1331122094327953No Hit
GAGCAGGACAGCAAGGACAGCACCTACAGCCTCAGCAGCACCCTGACGCT11840.12960925655298491No Hit
CAGTTAGGACCCAGAGGGAACCATGGAAACCCCAGCGCAGCTTCTCTTCC11740.12851458377804414No Hit
GTGTACAGGTCCCCGGAGGCATCCTGGCTAGGTGGGAAGTTTCTGGCGGT11690.12796724739057377No Hit
CAATAATAGACTGCAAAATCTTCAGGCTCCAGTCCACTGATGGTGAGAGTGAAGTCTGTCCCAGACCCACTGCCA11450.12534003273071598No Hit
GTTCAAGGACCACCTGAGCGTGACCTCTGTGGCTGCTACAGCGTGTCCAG11420.12501163089823375No Hit
GGCTGTAGGTGCTGTCCTTGCTGTCCTGCTCTGTGACACTCTCCTGGGAGTTACCCGATTGGAGGGCGTTATCCA11340.12413589267828114No Hit
GTGGCTGCACCATCTGTCTTCATCTTCCCGCCATCTGATGAGCAGTTGAAATCTGGAACTGCCTCTGTTGTGTGC11330.12402642540078708No Hit
GGCCAAAGGATGGGAGGGGGTCAGGCTGGAACTGAGGAGCAGGTGGGGGCACTTCTCCCTCTAACACTCTCCC11150.12205601440589371No Hit
CTCCTGTGCAGCCTCCGGATTCTCCTTTAGTACTAACTGGATGAACTGGGTCCGCCAAGCTCCGGGGAAGGGG10900.11931933246854184No Hit
GCTCAGTTAGGACCCAGAGGGAACCATGGAAACCCCAGCGCAGCTTCTCT10890.11920986519104776No Hit
ATCCCAGAGAGGCCAAAGTACAGTGGAAGGTGGATAACGCCCTCCAATCG10810.11833412697109516No Hit
CTTATACACATCTCCGAGCCCACGAGACGGACTCCTATCTCGTATGCCGT10710.11723945419615442No Hit
GAGTGAAGTCTGTCCCAGACCCACTGCCACTGAACCTGTCTGGGATGCCA10520.115159575923767No Hit
GTGCAGCCACAGTTCGTCTGATATCCACTTTGGTCCCAGGGCCGAAAGTG10380.11362703403884993No Hit
GACTTCACTCTCACCATCAGTGGACTGGAGCCTGAAGATTTTGCAGTCTATTATTGTCAATACTATTATAACTCT10260.11231342670892104No Hit
ATAATAGACTGCAAAATCTTCAGGCTCCAGTCCACTGATGGTGAGAGTGAAGTCTGTCCCAGACCCACTGCCA10190.1115471557664625No Hit
GCCTGGTACCAGCAGAAACCTGGCCAGGGTCCCAGGCTCCTCATCTATGGTACATCCAAGAGGGCCACTGGCATC10180.11143768848896843No Hit
GTATTGATACGTGTGATCCACTCCAGCCCCTTCCCCGGAGCTTGGCGGAC10080.11034301571402767No Hit
ATCCAAGAGGGCCACTGGCATCCCAGACAGGTTCAGTGGCAGTGGGTCTG9760.10684006283421728No Hit
GGCCCTGATGGGTGACTTCGCAGGCGTAGACTTTGTGTTTCTCGTAGTCTGCTTTGCTCAGCGTCAGGGTGCTG9760.10684006283421728No Hit
CTACATAGCCTGGTACCAGCAGAAACCTGGCCAGGGTCCCAGGCTCCTCATCTATGGTACATCCAAGAGGGCCA9760.10684006283421728No Hit
GCTTTGCTCAGCGTCAGGGTGCTGCTGAGGCTGTAGGTGCTGTCCTTGCT9650.10563592278178245No Hit
GCTCAGTAGCAGGTGCCGTCCACCTCCGCCATGACAACAGACACATTGAC9590.10497911911681801No Hit
GTGTGACCTGGAGCGAAAGCGGACAGAACGTGACCGCCAGAAACTTCCCACCTAGCCAGGATGCCTCCGGGGAC9550.10454125000684171No Hit
GCCTGGGACCCTGGCCAGGTTTCTGCTGGTACCAGGCTATGTAGTTGCTA9380.10268030628944243No Hit
GTTGTGTGCCTGCTGAATAACTTCTATCCCAGAGAGGCCAAAGTACAGTG9360.10246137173445428No Hit
GTCTATTATTGTCAATACTATTATAACTCTCAATTCACTTTCGGCCCTGGGACCAAAGTGGATATCAGACGAA9340.10224243717946613No Hit
GTCCTTGCTGTCCTGCTCTGTGACACTCTCCTGGGAGTTACCCGATTGGAGGGCGTTATCCACCTTCCACTGTA9340.10224243717946613No Hit
TCCATGTACCAACCCCCTCTTGTACAGTAATACACAGCCGTGTCCTCGGC9210.10081936257204316No Hit
GCCCCAAGGTCTTCCCGCTGAGCCTCGACAGCACCCCCCAAGATGGGAAC9190.100600428017055No Hit
CTGCTACTCTGGCTCCCAGATATTACCGGAGAGATTGTGTTGACGCAGTC9150.1001625589070787No Hit

[OK]Adapter Content

Adapter graph

Produced by FastQC (version 0.11.9)