FastQCFastQC Report
Wed 27 Apr 2022
EGAF00004684447

Summary

[OK]Basic Statistics

MeasureValue
FilenameEGAF00004684447
File typeConventional base calls
EncodingSanger / Illumina 1.9
Total Sequences913515
Sequences flagged as poor quality0
Sequence length20-76
%GC54

[OK]Per base sequence quality

Per base quality graph

[OK]Per tile sequence quality

Per tile quality graph

[OK]Per sequence quality scores

Per Sequence quality graph

[FAIL]Per base sequence content

Per base sequence content

[FAIL]Per sequence GC content

Per sequence GC content graph

[OK]Per base N content

N content graph

[WARN]Sequence Length Distribution

Sequence length distribution

[FAIL]Sequence Duplication Levels

Duplication level graph

[WARN]Overrepresented sequences

SequenceCountPercentagePossible Source
CTCCTGGGAGTTACCCGATTGGAGGGCGTTATCCACCTTCCACTGTACTTTGGCCTCTCTGGGATAGAAGTTATT30410.33288999085948234No Hit
CTCTAACACTCTCCCCTGTTGAAGCTCTTTGTGACGGGCGAGCTCAGGCC24700.27038417541036547No Hit
GGGGGGGGGGGGGGGGGGGGGGGGGGGGGGGGGGGGGGGGGGGGGGGGGG22400.2452067015867282No Hit
CTCCCAGGAGAGTGTCACAGAGCAGGACAGCAAGGACAGCACCTACAGCC22040.24126587959694148No Hit
GTTAGGACCCAGAGGGAACCATGGAAACCCCAGCGCAGCTTCTCTTCCTCCTGCTACTCTGGCTCCCAGATATTA21730.23787239399462515No Hit
GGCGTAGACTTTGTGTTTCTCGTAGTCTGCTTTGCTCAGCGTCAGGGTGC20390.22320377881041908No Hit
CTCCTGCAGGGCCAGCCAGAGTGTTAATAGCAACTACATAGCCTGGTACC17830.19518015577193587No Hit
GGCTGGAACTGAGGAGCAGGTGGGGGCACTTCTCCCTCTAACACTCTCCC17440.19091093194966696No Hit
GTGTTAATAGCAACTACATAGCCTGGTACCAGCAGAAACCTGGCCAGGGTCCCAGGCTCCTCATCTATGGTACAT17380.19025412828470248No Hit
TATCAATACTGATGGGGGTAGTGCGACGTACGCGGACTCCGTGGAGGGCCGATTCACCGTCTCCAGAGACAACGC17030.18642277357240986No Hit
GTAATACACAGCCGTGTCCTCGGCTCTCAGACTGTTGATTTGCAGATATA16660.1823724843051291No Hit
GTCCTGCTCTGTGACACTCTCCTGGGAGTTACCCGATTGGAGGGCGTTATCCACCTTCCACTGTACTTTGGCCT16420.1797452696452713No Hit
GTCTGGGACAGACTTCACTCTCACCATCAGTGGACTGGAGCCTGAAGATT15830.17328670027312087No Hit
CAACAGAGGCAGTTCCAGATTTCAACTGCTCATCAGATGGCGGGAAGATG15080.16507665446106523No Hit
GTCTACGCCTGCGAAGTCACCCATCAGGGCCTGAGCTCGCCCGTCACAAA14790.16190210341373704No Hit
CTATTATTGTCAATACTATTATAACTCTCAATTCACTTTCGGCCCTGGGA14760.16157370158125484No Hit
GTGTATTACTGTACAAGAGGGGGTTGGTACATGGACGTCTGGGGCAAGGG14560.15938435603137333No Hit
GCGTCAGGGTGCTGCTGAGGCTGTAGGTGCTGTCCTTGCTGTCCTGCTCT14410.15774234686896219No Hit
GTCCCAGACCCACTGCCACTGAACCTGTCTGGGATGCCAGTGGCCCTCTT14260.15610033770655105No Hit
GTCTTCATCTTCCCGCCATCTGATGAGCAGTTGAAATCTGGAACTGCCTCTGTTGTGTGCCTGCTGAATAACTT14250.155990870429057No Hit
GTACAGTGGAAGGTGGATAACGCCCTCCAATCGGGTAACTCCCAGGAGAG14240.15588140315156293No Hit
GTCTTTGTCTCCGGGAGAAACAGGCGCCCTCTCCTGCAGGGCCAGCCAGA13770.1507364411093414No Hit
CTCCAGGCACCCTGTCTTTGTCTCCGGGAGAAACAGGCGCCCTCTCCTGC13550.14832816100447174No Hit
CAATAGGGGTAGGTCCCCTGTGGAAAAAGGGTCAGAGGCCAAAGGATGGGAGGGGGTCAGGCTGGAACTGAGGAG13490.1476713573395073No Hit
GGATGGGAGGGGGTCAGGCTGGAACTGAGGAGCAGGTGGGGGCACTTCTCCCTCTAACACTCTCCCCTGTTGAAG13270.14526307723463763No Hit
CTATTATAACTCTCAATTCACTTTCGGCCCTGGGACCAAAGTGGATATCAGACGAACTGTGGCTGCACCAT13080.1431831989622502No Hit
GAGCAGGACAGCAAGGACAGCACCTACAGCCTCAGCAGCACCCTGACGCT13010.1424169280197917No Hit
GTCCCAGGCTCCTCATCTATGGTACATCCAAGAGGGCCACTGGCATCCCA12880.1409938534123687No Hit
GTCTCCAGGCACCCTGTCTTTGTCTCCGGGAGAAACAGGCGCCCTCTCCTGCAGGGCCAGCCAGAGTGTTAATAG12680.13880450786248721No Hit
CCCTATATCTGCAAATCAACAGTCTGAGAGCCGAGGACACGGCTGTGTAT12670.13869504058499313No Hit
CTCTGGGATAGAAGTTATTCAGCAGGCACACAACAGAGGCAGTTCCAGAT12600.13792876964253462No Hit
CCACTGTACTTTGGCCTCTCTGGGATAGAAGTTATTCAGCAGGCACACAACAGAGGCAGTTCCAGATTTCAACTG12040.1317986021028664No Hit
GTGTGACCTGGAGCGAAAGCGGACAGAACGTGACCGCCAGAAACTTCCCACCTAGCCAGGATGCCTCCGGGGAC11900.13026606021794934No Hit
GTTCAAGGACCACCTGAGCGTGACCTCTGTGGCTGCTACAGCGTGTCCAG11750.12862405105553823No Hit
CAATAATAGACTGCAAAATCTTCAGGCTCCAGTCCACTGATGGTGAGAGTGAAGTCTGTCCCAGACCCACTGCCA11460.12544950000821006No Hit
GTGGCTGCACCATCTGTCTTCATCTTCCCGCCATCTGATGAGCAGTTGAAATCTGGAACTGCCTCTGTTGTGTGC11290.12358855629081077No Hit
GCTCAGTTAGGACCCAGAGGGAACCATGGAAACCCCAGCGCAGCTTCTCT11180.12238441623837594No Hit
CTCCTGTGCAGCCTCCGGATTCTCCTTTAGTACTAACTGGATGAACTGGGTCCGCCAAGCTCCGGGGAAGGGG11090.12139921074092927No Hit
GGCTGTAGGTGCTGTCCTTGCTGTCCTGCTCTGTGACACTCTCCTGGGAGTTACCCGATTGGAGGGCGTTATCCA11090.12139921074092927No Hit
GTGTACAGGTCCCCGGAGGCATCCTGGCTAGGTGGGAAGTTTCTGGCGGT11050.12096134163095296No Hit
CCCTGACGCTGAGCAAAGCAGACTACGAGAAACACAAAGTCTACGCCTGCGAAGTCACCCATCAGGGCCTGAGCT10970.12008560341100036No Hit
GTGCAGCCACAGTTCGTCTGATATCCACTTTGGTCCCAGGGCCGAAAGTG10850.11877199608107147No Hit
GATCAGGACTGCACACAGAGAACTCACCATGGAGTTTGGGCTGAGCTGGG10750.1176773233061307No Hit
CAGTTAGGACCCAGAGGGAACCATGGAAACCCCAGCGCAGCTTCTCTTCC10740.11756785602863665No Hit
ATCCCAGAGAGGCCAAAGTACAGTGGAAGGTGGATAACGCCCTCCAATCG10520.115159575923767No Hit
GTATTGATACGTGTGATCCACTCCAGCCCCTTCCCCGGAGCTTGGCGGAC10270.1124228939864151No Hit
GTCCTTGCTGTCCTGCTCTGTGACACTCTCCTGGGAGTTACCCGATTGGAGGGCGTTATCCACCTTCCACTGTA10220.11187555759894473No Hit
GACTTCACTCTCACCATCAGTGGACTGGAGCCTGAAGATTTTGCAGTCTATTATTGTCAATACTATTATAACTCT10150.11110928665648621No Hit
GTCGCTGCACCGACCGGCCCTCGAGGACCTGCTCTTAGGTTCAGAAGCGAACCTCACGTGCACACTGACTGGC10100.11056195026901583No Hit
GTTGTGTGCCTGCTGAATAACTTCTATCCCAGAGAGGCCAAAGTACAGTG9830.1076063337766758No Hit
GAGTGAAGTCTGTCCCAGACCCACTGCCACTGAACCTGTCTGGGATGCCA9770.10694953011171136No Hit
ATAATAGACTGCAAAATCTTCAGGCTCCAGTCCACTGATGGTGAGAGTGAAGTCTGTCCCAGACCCACTGCCA9740.10662112827922914No Hit
GTCACATCCTGGCTGGGATTCGTGTAGTGCTTCACGTGGCATGTCACGGA9670.10585485733677061No Hit
GCCTGGTACCAGCAGAAACCTGGCCAGGGTCCCAGGCTCCTCATCTATGGTACATCCAAGAGGGCCACTGGCATC9650.10563592278178245No Hit
CTACATAGCCTGGTACCAGCAGAAACCTGGCCAGGGTCCCAGGCTCCTCATCTATGGTACATCCAAGAGGGCCA9650.10563592278178245No Hit
GAGTTTATTCAGGGGTGGGGACAGGCGGGTGGCTCAGTAGCAGGTGCCGT9630.10541698822679431No Hit
GCCTGGGACCCTGGCCAGGTTTCTGCTGGTACCAGGCTATGTAGTTGCTA9400.10289924084443058No Hit
GTCCAGGGCTTCTTCCCCCAGGAGCCACTCAGTGTGACCTGGAGCGAAAGCGGACAGAACGTGACCGCCAGAAA9240.10114776440452537No Hit

[OK]Adapter Content

Adapter graph

Produced by FastQC (version 0.11.9)