FastQCFastQC Report
Sat 23 Apr 2022
EGAF00004684448

Summary

[OK]Basic Statistics

MeasureValue
FilenameEGAF00004684448
File typeConventional base calls
EncodingSanger / Illumina 1.9
Total Sequences1174040
Sequences flagged as poor quality0
Sequence length20-76
%GC55

[OK]Per base sequence quality

Per base quality graph

[OK]Per tile sequence quality

Per base quality graph

[OK]Per sequence quality scores

Per Sequence quality graph

[FAIL]Per base sequence content

Per base sequence content

[FAIL]Per sequence GC content

Per sequence GC content graph

[OK]Per base N content

N content graph

[WARN]Sequence Length Distribution

Sequence length distribution

[FAIL]Sequence Duplication Levels

Duplication level graph

[WARN]Overrepresented sequences

SequenceCountPercentagePossible Source
GCCCAAGGCTGCCCCCTCGGTCACTCTGTTCCCGCCCTCCTCTGAGGAGC60870.5184661510681067No Hit
GCCTTGGGCTGACCGAGGACGGCCAGCTTGGTCCCTCCGCCAAATACCAAATTGTTGCTGCCTGCATATGAGCTG48670.4145514633232258No Hit
GTCCTGGGCCCAGTCTGCCCTGACTCAGCCTCCCTCCGCGTCCGGGTCTCCTGGACAGTCAGTCACCATCTCCTG37940.3231576436918674No Hit
GTGTGGTGGTCTCCACTCCCGCCTTGACGGGGCTGCTATCTGCCTTCCAG33460.28499880753637014No Hit
CCTCTGAGGAGCTTCAAGCCAACAAGGCCACACTGGTGTGTCTCATAAGT32370.27571462641817995No Hit
CCTCAGAGGAGGGCGGGAACAGAGTGACCGAGGGGGCAGCCTTGGGCTGACCGAGGACGGCCAGCTTGGTCCCTC28800.2453068038567681No Hit
GATTATTACTGCAGCTCATATGCAGGCAGCAACAATTTGGTATTTGGCGGAGGGACCAAGCTGGCCGTCCTCGGT28360.24155906102006747No Hit
CTCCTGGACAGTCAGTCACCATCTCCTGCACTGGAACCAGCAGTGACGTT27630.23534121495008686No Hit
GTACCAACACCACCCAGGCAAAGCCCCCAAACTCATGATTTATGAGGTCA27130.23108241627201798No Hit
GTGCAGGGAGAAGGGCTTGATGCCTTGGGGTGGGAGGAGAGACCCCTCCCCTGGGATCCTGCAGCTCTAGTCTCC25750.21932813192054784No Hit
CCTATGAACATTCTGTAGGGGCCACTGTCTTCTCCACGGTGCTCCCTTCATGCGTGACCTGGCAGCTGTAGCTT24970.2126844059827604No Hit
GGGCACAGGGTCCTGGGCCCAGTCTGCCCTGACTCAGCCTCCCTCCGCGTCCGGGTCTCCTGGACAGTCAGTCAC24650.20995877482879627No Hit
GTCTTCTCCACGGTGCTCCCTTCATGCGTGACCTGGCAGCTGTAGCTTCT24390.20774419951620046No Hit
GTGCTCCCTTCATGCGTGACCTGGCAGCTGTAGCTTCTGTGGGACTTCCACTGCTCAGGCGTCAGGCTCAGGTAG22680.19317910803720487No Hit
GTTTGGAGGGTGTGGTGGTCTCCACTCCCGCCTTGACGGGGCTGCTATCTGCCTTCCAGGCCACTGTCACGGCTC22560.19215699635446834No Hit
GTGTTGGTACCAGGAGACATAGTTATAACCACCAACGTCACTGCTGGTTCCAGTGCAGGAGATGGTGACTGA22460.19130523661885457No Hit
TCTCTGGGCTCCAGGCTGAGGATGAGGCTGATTATTACTGCAGCTCATAT21460.18278763926271677No Hit
GGGTTTATTGAGTGCAGGGAGAAGGGCTTGATGCCTTGGGGTGGGAGGAGAGACCCCTCCCCTGGGATCCTGCAG21170.18031753602943681No Hit
CTCTGTTCCCGCCCTCCTCTGAGGAGCTTCAAGCCAACAAGGCCACACTGGTGTGTCTCATAAGTGACTTCTACC21010.17895472045245478No Hit
CTTCATGCGTGACCTGGCAGCTGTAGCTTCTGTGGGACTTCCACTGCTCAGGCGTCAGGCTCAGGTAGCTGCTGG20860.1776770808490341No Hit
CTCCTGCACTGGAACCAGCAGTGACGTTGGTGGTTATAACTATGTCTCCTGGTACCAACACCACCCAGGCAAAGC20620.17563285748356106No Hit
GTCAGTCACCATCTCCTGCACTGGAACCAGCAGTGACGTTGGTGGTTATAACTATGTCTCCTGGTACCAACACCA20520.17478109774794726No Hit
CACCAGTGTGGCCTTGTTGGCTTGAAGCTCCTCAGAGGAGGGCGGGAACAGAGTGACCGAGGGGGCAGCCTTGGG20260.17256652243535145No Hit
GTAATAATCAGCCTCATCCTCAGCCTGGAGCCCAGAGACGGTCAGGGAGG20210.17214064256754455No Hit
GCTCAGGCGTCAGGCTCAGGTAGCTGCTGGCCGCGTACTTGTTGTTGCTTTGTTTGGAGGGTGTGGTGGTCTCCA19820.1688187795986508No Hit
GGTTTATTGAGTGCAGGGAGAAGGGCTTGATGCCTTGGGGTGGGAGGAGAGACCCCTCCCCTGGGATCCTGCAG18850.16055671016319717No Hit
CTTATGAGACACACCAGTGTGGCCTTGTTGGCTTGAAGCTCCTCAGAGGA18340.1562127355115669No Hit
GAGAAGGGCTTGATGCCTTGGGGTGGGAGGAGAGACCCCTCCCCTGGGAT17740.15110217709788423No Hit
GTTCCCGCCCTCCTCTGAGGAGCTTCAAGCCAACAAGGCCACACTGGTGT17710.1508466491772001No Hit
GGCCTGGGCTCTGCTCCTCCTCACCCTCCTCACTCAGGGCACAGGGTCCTGGGCCCAGTCTGCCCTGACTCAGC17690.15067629723007733No Hit
CCCTTCATGCGTGACCTGGCAGCTGTAGCTTCTGTGGGACTTCCACTGCTCAGGCGTCAGGCTCAGGTAGCTG17380.14803584204967463No Hit
CCACCACACCCTCCAAACAAAGCAACAACAAGTACGCGGCCAGCAGCTAC16930.14420292323941264No Hit
CCCCTGAGGGCCGCTTAATGACCTCATAAATCATGAGTTTGGGGGCTTTGCCTGGGTGGTGTTGGTACCAGGAGA16430.13994412456134372No Hit
TCCTCACCCTCCTCACTCAGGGCACAGGGTCCTGGGCCCAGTCTGCCCTGACTCAGCCTCCCTCCGCGTCCGGGT16390.13960342066709822No Hit
GGTTATAACTATGTCTCCTGGTACCAACACCACCCAGGCAAAGCCCCCAAACTCATGATTTATGAGGTCATTAAG16320.1390071888521686No Hit
GGCTCAGGTAGCTGCTGGCCGCGTACTTGTTGTTGCTTTGTTTGGAGGGTGTGGTGGTCTCCACTCCCGCCTTGA15910.13551497393615208No Hit
ACACTGGTGTGTCTCATAAGTGACTTCTACCCGGGAGCCGTGACAGTGGC15810.1346632142005383No Hit
GCCTTGGGGTGGGAGGAGAGACCCCTCCCCTGGGATCCTGCAGCTCTAGT15720.1338966304384859No Hit
GGAACAGAGTGACCGAGGGGGCAGCCTTGGGCTGACCGAGGACGGCCAGC15640.13321522264999489No Hit
GCCCAGAGACGGTCAGGGAGGCCGTGTTGCCAGACTTGGAGCCAGAGAAG15630.13313004667643352No Hit
GTCAGGGAGGCCGTGTTGCCAGACTTGGAGCCAGAGAAGCGATCAGGGAC15560.13253381486150387No Hit
GGATGAGGCTGATTATTACTGCAGCTCATATGCAGGCAGCAACAATTTGGTATTTGGCGGAGGGACCAAGCTGGC15530.13227828694081972No Hit
GCTATCTGCCTTCCAGGCCACTGTCACGGCTCCCGGGTAGAAGTCACTTA15520.13219311096725836No Hit
ACCATGGCCTGGGCTCTGCTCCTCCTCACCCTCCTCACTCAGGGCACAGGGTCCTGGGCCCAGTCTGCCCTGACT15490.1319375830465742No Hit
GACTCAGCCTCCCTCCGCGTCCGGGTCTCCTGGACAGTCAGTCACCATCT15180.1292971278661715No Hit
GCCCTCAGGGGTCCCTGATCGCTTCTCTGGCTCCAAGTCTGGCAACACGG15160.12912677591904875No Hit
GCTCTAGTCTCCCGTGGTGGGGGTGAGGGTTGAGAACCTATGAACATTCT15060.12827501618343498No Hit
GTAATACATGGCCGTGTCCTCAGATCTTAGGCTGCTCAGCTCCACGTAGG14130.12035365064222683No Hit
GCCCTGACTCAGCCTCCCTCCGCGTCCGGGTCTCCTGGACAGTCAGTCAC14060.1197574188272972No Hit
GAGTGACCGAGGGGGCAGCCTTGGGCTGACCGAGGACGGCCAGCTTGGTCCCTCCGCCAAATACCAAATTGTTG13940.11873530714456065No Hit
AGTCTGCCCTGACTCAGCCTCCCTCCGCGTCCGGGTCTCCTGGACAGTCA13770.11728731559401723No Hit
GGTGTGTCTCATAAGTGACTTCTACCCGGGAGCCGTGACAGTGGCCTGGA13750.1171169636468945No Hit
TCCTCACTCAGGGCACAGGGTCCTGGGCCCAGTCTGCCCTGACTCAGCCT13570.11558379612278968No Hit
GCCTCCCTCCGCGTCCGGGTCTCCTGGACAGTCAGTCACCATCTCCTGCA13560.11549862014922832No Hit
GCTGTAGCTTCTGTGGGACTTCCACTGCTCAGGCGTCAGGCTCAGGTAGC13410.11422098054580765No Hit
CCCCTGGGATCCTGCAGCTCTAGTCTCCCGTGGTGGGGGTGAGGGTTGAG13380.1139654526251235No Hit
CTCAGGACGTCACCACCATGGCCTGGGCTCTGCTCCTCCTCACCCTCCTC13320.11345439678375523No Hit
CCTCAGGGGTCCCTGATCGCTTCTCTGGCTCCAAGTCTGGCAACACGGCCTCCCTGACCGTCTCTGGGCTCCAGG13320.11345439678375523No Hit
GGTAGCTGCTGGCCGCGTACTTGTTGTTGCTTTGTTTGGAGGGTGTGGTGGTCTCCACTCCCGCCTTGACGGGG12960.11038806173554565No Hit
CTCCCTGACCGTCTCTGGGCTCCAGGCTGAGGATGAGGCTGATTATTACT12920.11004735784130014No Hit
CTTCTCCACGGTGCTCCCTTCATGCGTGACCTGGCAGCTGTAGCTTCTGT12740.10851419031719532No Hit
GGGCTGCTATCTGCCTTCCAGGCCACTGTCACGGCTCCCGGGTAGAAGTCACTTATGAGACACACCAGTGTGGC12660.10783278252870429No Hit
GACTTCCACTGCTCAGGCGTCAGGCTCAGGTAGCTGCTGGCCGCGTACTT12660.10783278252870429No Hit
GTTGAGAACCTATGAACATTCTGTAGGGGCCACTGTCTTCTCCACGGTGCTCCCTTCATGCGTGACCTGGCAG12620.1074920786344588No Hit
TTCCAGGCCACTGTCACGGCTCCCGGGTAGAAGTCACTTATGAGACACACCAGTGTGGCCTTGTTGGCTTGAAG12530.10672549487240639No Hit
CTTATACACATCTCCGAGCCCACGAGACTAGGCATGATCTCGTATGCCGT12440.10595891111035399No Hit
CTGTAGGGGCCACTGTCTTCTCCACGGTGCTCCCTTCATGCGTGACCTGG12400.10561820721610847No Hit
CCCGTGGTGGGGGTGAGGGTTGAGAACCTATGAACATTCTGTAGGGGCCA12130.10331845592995129No Hit
GTGTGGCCTTGTTGGCTTGAAGCTCCTCAGAGGAGGGCGGGAACAGAGTGACCGAGGGGGCAGCCTTGGGCTGAC12100.10306292800926714No Hit
GTTATAACCACCAACGTCACTGCTGGTTCCAGTGCAGGAGATGGTGACTG11990.10212599230009199No Hit
ATGTATTACTGTGCGAGAGATCTATCTCCCCCCGGATTTTGTAGTGGTGG11960.10187046437940786No Hit
CTTAATGACCTCATAAATCATGAGTTTGGGGGCTTTGCCTGGGTGGTGTT11930.10161493645872371No Hit
GTCATTAAGCGGCCCTCAGGGGTCCCTGATCGCTTCTCTGGCTCCAAGTCTGGCAACACGGCCTCCCTGACCGT11890.1012742325644782No Hit
CATATGCAGGCAGCAACAATTTGGTATTTGGCGGAGGGACCAAGCTGGCCGTCCTCGGTCAGCCCAAGGCTGCCC11810.1005928247759872No Hit

[OK]Adapter Content

Adapter graph

[WARN]Kmer Content

Kmer graph

SequenceCountPValueObs/Exp MaxMax Obs/Exp Position
CGTTTAA150.002280714769.56406447
CGTGTTA200.00609724154.27059666
TCGGCTA200.00694092752.5212352
TCTCGTA1500.048.20814538
AGGGGGG2850.048.04553670
TCGTATG1850.044.78306240
CGTATGC1900.043.6383441
GGTATCA10450.043.5305561
CGAGCTT608.336749E-443.4697770
AAAACCG1407.712515E-1041.9172870
GTGGTAT2600.040.781
CGAGACT2100.040.71041523
ATCTCGT1700.040.47805837
ACGAGAC2100.039.08199722
CTTATAC2300.037.1767121
GTATCAA26900.037.1269651
ATGCCGT2250.036.9646944
GACTAGG2250.036.4858626
CCCACGA2350.036.3733219
TGGTATC2950.035.9263842
Produced by FastQC (version 0.11.5)