FastQCFastQC Report
Sat 23 Apr 2022
EGAF00004684451

Summary

[OK]Basic Statistics

MeasureValue
FilenameEGAF00004684451
File typeConventional base calls
EncodingSanger / Illumina 1.9
Total Sequences784729
Sequences flagged as poor quality0
Sequence length20-76
%GC53

[OK]Per base sequence quality

Per base quality graph

[OK]Per tile sequence quality

Per base quality graph

[OK]Per sequence quality scores

Per Sequence quality graph

[FAIL]Per base sequence content

Per base sequence content

[FAIL]Per sequence GC content

Per sequence GC content graph

[OK]Per base N content

N content graph

[WARN]Sequence Length Distribution

Sequence length distribution

[FAIL]Sequence Duplication Levels

Duplication level graph

[WARN]Overrepresented sequences

SequenceCountPercentagePossible Source
GTATTACACTACTCCTCGAACTTTTGGCCAGGGGACCAAGCTGGAGATCAAACGAACTGTGGCTGCACCAT70930.9038789187095162No Hit
GTGTAATACTGCTGACAGTAATAAACTGCCACATCCTCAGCCTGCAGGCTGCTGATGGTGAGAGTGAAATCTGTC57190.7287866257013568No Hit
CTCCTGGGAGTTACCCGATTGGAGGGCGTTATCCACCTTCCACTGTACTTTGGCCTCTCTGGGATAGAAGTTATT39410.5022115915175812No Hit
CTCTAACACTCTCCCCTGTTGAAGCTCTTTGTGACGGGCGAGCTCAGGCC32320.4118619294049284No Hit
GTTTTATACACCTCCAACAATAAGAGCTACTTAGCTTGGTACCAACAAAA29890.3808958251829613No Hit
CTCCTAAGCTGCTCATTTACTGGGCATCTACCCGGGAATCCGGGGTCCCT28760.36649594955710824No Hit
GGGGGGGGGGGGGGGGGGGGGGGGGGGGGGGGGGGGGGGGGGGGGGGGGG25760.32826619125838347No Hit
CTCCCAGGAGAGTGTCACAGAGCAGGACAGCAAGGACAGCACCTACAGCC22190.282772778882901No Hit
GCTTAGGAGGCTGTCCTGGTTTTTGTTGGTACCAAGCTAAGTAGCTCTTATTGTTGGAGGTGTATAAAACACT22010.2804789933849775No Hit
GTCCTGCTCTGTGACACTCTCCTGGGAGTTACCCGATTGGAGGGCGTTATCCACCTTCCACTGTACTTTGGCCT19990.25473762279716944No Hit
GGCTGGAACTGAGGAGCAGGTGGGGGCACTTCTCCCTCTAACACTCTCCC19880.2533358649928829No Hit
CAACAGAGGCAGTTCCAGATTTCAACTGCTCATCAGATGGCGGGAAGATG19300.2459447783884628No Hit
GGCGTAGAGTTTGTGTTTCTCGTAGTCTGCTTTGCTCAGCGTCAGGGTGC18600.23702450145209364No Hit
GTAATATACAGCCGTGTCCTCGGCTCTCAGGCTGTTCATTTGCAGATACA17890.22797679198806212No Hit
GCGTCAGGGTGCTGCTGAGGCTGTAGGTGCTGTCCTTGCTGTCCTGCTCT17150.21854678494104335No Hit
GTACAGTGGAAGGTGGATAACGCCCTCCAATCGGGTAACTCCCAGGAGAG15660.19955933831934336No Hit
GGATGGGAGGGGGTCAGGCTGGAACTGAGGAGCAGGTGGGGGCACTTCTCCCTCTAACACTCTCCCCTGTTGAAG15570.19841244557038162No Hit
GTCTTCATCTTCCCGCCATCTGATGAGCAGTTGAAATCTGGAACTGCCTCTGTTGTGTGCCTGCTGAATAACTT15220.19395230710219707No Hit
GTTTATTACTGTCAGCAGTATTACACTACTCCTCGAACTTTTGGCCAGGGGACCAAGCTGGAGATCAAACGAA15120.1926779818255729No Hit
CTCTGGGATAGAAGTTATTCAGCAGGCACACAACAGAGGCAGTTCCAGAT14790.18847270841271319No Hit
CCACTGTACTTTGGCCTCTCTGGGATAGAAGTTATTCAGCAGGCACACAACAGAGGCAGTTCCAGATTTCAACTG14740.1878355457744011No Hit
CAATAGGGGTAGGTCCCCTGTGGAAAAAGGGTCAGAGGCCAAAGGATGGGAGGGGGTCAGGCTGGAACTGAGGAG14470.18439486752751588No Hit
GAGCAGGACAGCAAGGACAGCACCTACAGCCTCAGCAGCACCCTGACGCT14160.18044445916998098No Hit
GGCTGTAGGTGCTGTCCTTGCTGTCCTGCTCTGTGACACTCTCCTGGGAGTTACCCGATTGGAGGGCGTTATCCA14050.1790427013656944No Hit
GTGGCTGCACCATCTGTCTTCATCTTCCCGCCATCTGATGAGCAGTTGAAATCTGGAACTGCCTCTGTTGTGTGC13780.17560202311880918No Hit
GTGTTGCAGACCCAGGTCTTCATTTCTCTGTTGCTCTGGATCTCTGGTGC13610.1734356701485481No Hit
GTAATAAACTGCCACATCCTCAGCCTGCAGGCTGCTGATGGTGAGAGTGA13250.16884809915270113No Hit
GTGCAGCCACAGTTCGTTTGATCTCCAGCTTGGTCCCCTGGCCAAAAGTT12790.16298620288023No Hit
CCCTGACGCTGAGCAAAGCAGACTACGAGAAACACAAACTCTACGCCTGCGAAGTCACCCATCAGGGCCTGAGCT12630.16094728243763134No Hit
GATGAAGACAGATGGTGCAGCCACAGTTCGTTTGATCTCCAGCTTGGTCC12550.159927822216332No Hit
CTCTACGCCTGCGAAGTCACCCATCAGGGCCTGAGCTCGCCCGTCACAAA12510.15941809210568234No Hit
GTCCCTGACCGATTCGGTGGCAGCGGGTCTGGGACAGATTTCACTCTCAC12300.15674200902477162No Hit
GTCTCCAGACTCCCTGGCTGTGTCTCTGGGCGAGAGGGCCACCATCAACTGCAAGTCCAGCCAGAGTGTTTTATA12280.15648714396944677No Hit
TCCTCAGCCTGCAGGCTGCTGATGGTGAGAGTGAAATCTGTCCCAGACCCGCTGCCACCGAATCGGTCAGGGACC11810.15049781516931324No Hit
ATCCCAGAGAGGCCAAAGTACAGTGGAAGGTGGATAACGCCCTCCAATCG11590.14769429956074007No Hit
GTCCCAGACCCGCTGCCACCGAATCGGTCAGGGACCCCGGATTCCCGGGT11370.14489078395216692No Hit
GTCCTTGCTGTCCTGCTCTGTGACACTCTCCTGGGAGTTACCCGATTGGAGGGCGTTATCCACCTTCCACTGTA11120.1417049707606065No Hit
GTATAAAACACTCTGGCTGGACTTGCAGTTGATGGTGGCCCTCTCGCCCAGAGACACAGCCAGGGAGTCTGGAGA10970.1397934828456703No Hit
GATTTCACTCTCACCATCAGCAGCCTGCAGGCTGAGGATGTGGCAGTTTATTACTGTCAGCAGTATTACACTACT10680.13609793954346022No Hit
GCTTTGCTCAGCGTCAGGGTGCTGCTGAGGCTGTAGGTGCTGTCCTTGCT10610.13520591184982333No Hit
GGCCAAAGGATGGGAGGGGGTCAGGCTGGAACTGAGGAGCAGGTGGGGGCACTTCTCCCTCTAACACTCTCCC10600.13507847932216088No Hit
GTACTTTGGCCTCTCTGGGATAGAAGTTATTCAGCAGGCACACAACAGAGGCAGTTCCAGATTTCAACTGCTCAT10570.13469618173917366No Hit
GTATATTACTGTGTGAGAGATCCACATAGGGGGGGTGACTATGACCTCTGGGGCCAGGGAACCCTGGTCACCGT10560.1345687492115112No Hit
GTTGTGTGCCTGCTGAATAACTTCTATCCCAGAGAGGCCAAAGTACAGTG10510.13393158657319915No Hit
CCTACAGCCTCAGCAGCACCCTGACGCTGAGCAAAGCAGACTACGAGAAACACAAACTCTACGCCTGCGAAGTCA10270.13087320590930118No Hit
CTCCCTGGCTGTGTCTCTGGGCGAGAGGGCCACCATCAACTGCAAGTCCAGCCAGAGTGTTTTATACACCTCCAA10240.1304909083263139No Hit
GAGTGAAATCTGTCCCAGACCCGCTGCCACCGAATCGGTCAGGGACCCCG10180.12972631316033942No Hit
GGCCCTGATGGGTGACTTCGCAGGCGTAGAGTTTGTGTTTCTCGTAGTCTGCTTTGCTCAGCGTCAGGGTGCTG9940.12666793249644145No Hit
GCTCATTTACTGGGCATCTACCCGGGAATCCGGGGTCCCTGACCGATTCGGTGGCAGCGGGTCTGGGACAGATTT9780.12462901205384279No Hit
ACCTTCCACTGTACTTTGGCCTCTCTGGGATAGAAGTTATTCAGCAGGCA9630.12271752413890655No Hit
CCCTGGCTGTGTCTCTGGGCGAGAGGGCCACCATCAACTGCAAGTCCAGC9290.11838481819838442No Hit
GCACACAACAGAGGCAGTTCCAGATTTCAACTGCTCATCAGATGGCGGGA9170.11685562786643543No Hit
GTCAGGGACCCCGGATTCCCGGGTAGATGCCCAGTAAATGAGCAGCTTAGGAGGCTGTCCTGGTTTTTGTTGGTA9160.11672819533877302No Hit
GGCTGAGGATGTGGCAGTTTATTACTGTCAGCAGTATTACACTACTCCTCGAACTTTTGGCCAGGGGACCAAG9020.11494413995149919No Hit
GTAAATGAGCAGCTTAGGAGGCTGTCCTGGTTTTTGTTGGTACCAAGCTA8940.11392467973019985No Hit
CCTCTGTTGTGTGCCTGCTGAATAACTTCTATCCCAGAGAGGCCAAAGTA8910.1135423821472126No Hit
GTTGAAATCTGGAACTGCCTCTGTTGTGTGCCTGCTGAATAACTTCTATC8820.11239548939825086No Hit
CTTCTATCCCAGAGAGGCCAAAGTACAGTGGAAGGTGGATAACGCCCTCCAATCGGGTAACTCCCAGGAGAGTGT8820.11239548939825086No Hit
CCCCTGTTGAAGCTCTTTGTGACGGGCGAGCTCAGGCCCTGATGGGTGACTTCGCAGGCGTAGAGTTTGTGTTT8720.11112116412162672No Hit
ACTTAGCTTGGTACCAACAAAAACCAGGACAGCCTCCTAAGCTGCTCATT8700.11086629906630187No Hit
GTAGTGTAATACTGCTGACAGTAATAAACTGCCACATCCTCAGCCTGCAGGCTGCTGATGGTGAGAGTGAAAT8670.11048400148331461No Hit
GTGTCACAGAGCAGGACAGCAAGGACAGCACCTACAGCCTCAGCAGCACC8530.1086999460960408No Hit
GTTGAAGCTCTTTGTGACGGGCGAGCTCAGGCCCTGATGGGTGACTTCGC8470.1079353509300663No Hit
GAGCAAAGCAGACTACGAGAAACACAAACTCTACGCCTGCGAAGTCACCC8340.1062787280704549No Hit
GTTATGACCCAGTCTCCAGACTCCCTGGCTGTGTCTCTGGGCGAGAGGGC8150.10385751004486898No Hit
GTGGAAGGTGGATAACGCCCTCCAATCGGGTAACTCCCAGGAGAGTGTCACAGAGCAGGACAGCAAGGACAGCAC8130.10360264498954416No Hit
GTCTCTGGGCGAGAGGGCCACCATCAACTGCAAGTCCAGCCAGAGTGTTTTATACACCTCCAACAATAAGAGCTA8130.10360264498954416No Hit
GCCTCTGTTGTGTGCCTGCTGAATAACTTCTATCCCAGAGAGGCCAAAGTACAGTGGAAGGTGGATAACGCCCTC8030.10232831971292No Hit
TTGCTGTCCTGCTCTGTGACACTCTCCTGGGAGTTACCCGATTGGAGGGC7990.10181858960227033No Hit
GAGTTTGTGTTTCTCGTAGTCTGCTTTGCTCAGCGTCAGGGTGCTGCTGAGGCTGTAGGTGCTGTCCTTGCTGTC7950.10130885949162066No Hit
ACACTACTCCTCGAACTTTTGGCCAGGGGACCAAGCTGGAGATCAAACGA7930.10105399443629584No Hit
GGTGAAAGATGAGCTGGAGGACCGCAATAGGGGTAGGTCCCCTGTGGAAA7920.10092656190863343No Hit

[OK]Adapter Content

Adapter graph

[WARN]Kmer Content

Kmer graph

SequenceCountPValueObs/Exp MaxMax Obs/Exp Position
CGAAACG200.00715742152.11318244
AGAGTCG306.9037237E-445.16837711
GTGGTAT2450.040.1547661
GGTATCA8800.039.320831
GTAGTTT700.00157401238.1971770
GTATCAA22800.038.089941
TGGTATC2500.037.9486962
CGCACTT400.002567532534.57712641
ACCCGCA400.002642945234.37362338
TATCAAC25600.033.882762
ATCAACG26650.032.535353
TCAACGC27150.031.936174
TATAAGA851.4149373E-731.8896562
CAACGCA27300.031.6386475
CGAGGCA450.004029931531.53745856
AACGCAG27600.031.4234836
ACGAACT17800.031.05337352
ACAGACG450.004416507730.95270346
CGAACTG18150.030.89776653
AACGAAC18700.030.63772451
Produced by FastQC (version 0.11.5)