FastQCFastQC Report
Sat 23 Apr 2022
EGAF00004684452

Summary

[OK]Basic Statistics

MeasureValue
FilenameEGAF00004684452
File typeConventional base calls
EncodingSanger / Illumina 1.9
Total Sequences792862
Sequences flagged as poor quality0
Sequence length20-76
%GC55

[OK]Per base sequence quality

Per base quality graph

[OK]Per tile sequence quality

Per base quality graph

[OK]Per sequence quality scores

Per Sequence quality graph

[FAIL]Per base sequence content

Per base sequence content

[FAIL]Per sequence GC content

Per sequence GC content graph

[OK]Per base N content

N content graph

[WARN]Sequence Length Distribution

Sequence length distribution

[FAIL]Sequence Duplication Levels

Duplication level graph

[WARN]Overrepresented sequences

SequenceCountPercentagePossible Source
CTCCTGGGAGTTACCCGATTGGAGGGCGTTATCCACCTTCCACTGTACTTTGGCCTCTCTGGGATAGAAGTTATT20600.25981822814058436No Hit
GAAATACACAGCCGAGTCCGCGGCGTTCACAGAGCTCAACTTCAGGGAGA20400.2572957210712583No Hit
GTGCAGTGACAGTCGGGGGTGGCAGCATGAGGGAGATGGGGTAGGTGGAG18940.23888141946517805No Hit
CTCTAACACTCTCCCCTGTTGAAGCTCTTTGTGACGGGCGAGCTCAGGCC18250.23017877007600313No Hit
GTGTATTTCTGTGCGAGAGGCCCGTTTTTATATGGTTCGGGGAGACCTCCGTTTGACTCCTGGGGCCAGGGAACC15880.2002870613044893No Hit
GTCACTGCACCGACCGGCCCTCGAGGACCTGCTCTTAGGTTCAGAAGCGAACCTCACGTGCACACTGACCGGC15790.19915193312329257No Hit
GTTAGGACCCAGAGGGAACCATGGAAACCCCAGCGCAGCTTCTCTTCCTCCTGCTACTCTGGCTCCCAGATACCA15570.19637717534703392No Hit
GTCACATCCTGGCTGGGATTCGTGTAGTGCTTCACGTGGCATGTCACGGA15520.1957465485797024No Hit
CTTATACACATCTCCGAGCCCACGAGACCGTACTAGATCTCGTATGCCGT15000.18918803019945465No Hit
GTGTACAGGTCCCCGGAGGCATCCTGGCTGGGTGGGAAGTTTCTGGCGGT14410.18174663434494276No Hit
GTGTGACCTGGAGCGAAAGCGGACAGGGCGTGACCGCCAGAAACTTCCCACCCAGCCAGGATGCCTCCGGGGAC14320.18061150616374602No Hit
ACCCTGGCCAGGCTCCCAGGCTCCTCATCTATGGTGCATCCAGCAGGGCC14200.1790980019221504No Hit
GTTCAAGGACCACCTGAGCGTGACCTCTGTGGCTGCTACAGCGTGTCCAG13680.17253948354190263No Hit
TCCTTGGGGCTGAAGCCACGTGCCAGGCACGTCAGCGTCACCAGCTCGTT13620.1717827314211048No Hit
GTATGCTGGTCACAGCGAAGGTGGTGGTGCCCTGGCTGGGCTCCTGCCGG13260.1672422186963179No Hit
GGCGTAGACTTTGTGTTTCTCGTAGTCTGCTTTGCTCAGCGTCAGGGTGC13250.1671160933428516No Hit
GCCCCAAGGTCTTCCCGCTGAGCCTCTGCAGCACCCAGCCAGATGGGAAC12750.16080982566953644No Hit
GTCATGGACCTCCTGCACAAGAACATGAAACACCTGTGGTTCTTCCTCCTCCTGGTGGCAGCTCCCAGATGGGTC12670.15980082284180602No Hit
AGGTCACACTGAGTGGCTCCTGGGGGAAGAAGCCCTGGACCAGGCAGGCG12450.15702606506554734No Hit
GTCCAGGGCTTCTTCCCCCAGGAGCCACTCAGTGTGACCTGGAGCGAAAGCGGACAGGGCGTGACCGCCAGAAA12130.15299005375462565No Hit
GCTCAGTAGCAGGTGCCGTCCACCTCCGCCATGACAACAGACACATTGAC11840.14933241850410287No Hit
CATCTATGGTGCATCCAGCAGGGCCACTGGCATCCCAGACAGGTTCAGTGGCAGTGGCTCTGGGACAGACTTCA11820.14908016779717026No Hit
CTCCCAGGAGAGTGTCACAGAGCAGGACAGCAAGGACAGCACCTACAGCC11670.1471882874951757No Hit
GGATGTGACTGTGCCCTGCCCAGTTCCCTCAACTCCACCTACCCCATCTCCCTCAACTCCACCTACCCCATCTCC11530.14542253254664747No Hit
GGCTGGAACTGAGGAGCAGGTGGGGGCACTTCTCCCTCTAACACTCTCCC11460.14453965507238334No Hit
CTACTGGACCTGGATCCGCCAGTCCCCAGGGAAGGGGCCGGAGTGGATTGGGGAAATCAGTCACAGTGGAATCAC11280.1422693987099899No Hit
CAGTAGACCCGTCCAAGGACCAGTTCTCCCTGAAGTTGAGCTCTGTGAACGCCGCGGACTCGGCTGTGTATTT11160.14075589446839426No Hit
GTCTTGGACTCGGGGTAGGCAGCAGTGCAAGTGAAGGTCTTCCCATGGTT11140.14050364376146163No Hit
CGTGCACACTGACCGGCCTGAGAGATGCCTCAGGTGTCACCTTCACCTGGACGCCCTCAAGTGGGAAGAGCG10930.13785501133866926No Hit
GTCCTGCTCTGTGACACTCTCCTGGGAGTTACCCGATTGGAGGGCGTTATCCACCTTCCACTGTACTTTGGCCT10840.13671988315747255No Hit
CAACAGAGGCAGTTCCAGATTTCAACTGCTCATCAGATGGCGGGAAGATG10790.13608925639014105No Hit
GGATGGGAGGGGGTCAGGCTGGAACTGAGGAGCAGGTGGGGGCACTTCTCCCTCTAACACTCTCCCCTGTTGAAG10750.1355847549762758No Hit
CTCCTGCAGGGCCAGTCAGAGTGTTAGCAGCAGCTACTTAGCCTGGTACC10590.13356674932081497No Hit
GTGTGCACGTGAGGTTCGCTTCTGAACCTAAGAGCAGGTCCTCGAGGGCC10530.13280999720001715No Hit
GAGTTTATTCAGGGGTGGGGACAGGCGGGCGGCTCAGTAGCAGGTGCCGT10510.13255774649308455No Hit
GTGCCTAGCCGGCAAGTCCGTGACATGCCACGTGAAGCACTACACGAATCCCAGCCAGGATGTGACTGTGCCCTG10230.12902623659602808No Hit
CAGTATGCTGGTCACAGCGAAGGTGGTGGTGCCCTGGCTGGGCTCCTGCC10180.12839560982869655No Hit
GGTCTACTGATATGGTGACTCGACTCTTGAGTGACGGGTTGTAGCTGGTG10160.12814335912176394No Hit
GTCTCCAGGCACCCTGTCTTTGTCTCCAGGGGAAAGAGCCACCCTCTCCTGCAGGGCCAGTCAGAGTGTTAGCAG9980.12587310275937047No Hit
CAATAGGGGTAGGTCCCCTGTGGAAAAAGGGTCAGAGGCCAAAGGATGGGAGGGGGTCAGGCTGGAACTGAGGAG9960.1256208520524379No Hit
GAGTGGCTCCTGGGGGAAGAAGCCCTGGACCAGGCAGGCGATGACCACGT9820.12385509710390963No Hit
GCGTCAGGGTGCTGCTGAGGCTGTAGGTGCTGTCCTTGCTGTCCTGCTCT9600.12108033932765097No Hit
GTGTCTGGTGGGTCCTTCAGTGGTTACTACTGGACCTGGATCCGCCAGTC9300.11729657872366188No Hit
GCTAGGCACTGTGTGGCCGGCAGGGTCAGCTGGCTGCTCGTGGTGTACAG9280.11704432801672927No Hit
GTACAGTGGAAGGTGGATAACGCCCTCCAATCGGGTAACTCCCAGGAGAG9190.11590919983553254No Hit
CTCCAGGCACCCTGTCTTTGTCTCCAGGGGAAAGAGCCACCCTCTCCTGC9130.11515244771473472No Hit
CCTTGGGGCTGGTCGGGGATGCTGAGGAGACGGTGACCGGGGTTCCCTGGCCCCAGGAGTCAAACGGAGGTCTCC9100.1147740716543358No Hit
GTACAGGTCCCCGGAGGCATCCTGGCTGGGTGGGAAGTTTCTGGCGGTCACGCCCTGTCCGCTTTCGCTCCAGGT9090.11464794630086951No Hit
GTCTACGCCTGCGAAGTCACCCATCAGGGCCTGAGCTCGCCCGTCACAAA9050.11414344488700429No Hit
GGTTACTACTGGACCTGGATCCGCCAGTCCCCAGGGAAGGGGCCGGAGTG9040.114017319533538No Hit
GTTTTACTGTCAGCAATATGGTAGTTCACCCTACACTTTTGGCCAGGGGA8950.11288219135234126No Hit
CCACTGTACTTTGGCCTCTCTGGGATAGAAGTTATTCAGCAGGCACACAACAGAGGCAGTTCCAGATTTCAACTG8890.11212543923154344No Hit
GCCCTGGACCAGGCAGGCGATGACCACGTTCCCATCTGGCTGGGTGCTGC8860.11174706317114452No Hit
GTCTTTGTCTCCAGGGGAAAGAGCCACCCTCTCCTGCAGGGCCAGTCAGA8500.10720655044635763No Hit
GTCCTGTCCCAGGTGCAGTTACAGCAGTGGGGCGCAGGACTGTTGAAGCC8420.1061975476186272No Hit
GACTTCACTCTCACCATCAGCAGACTGGAGCCTGAAGACTTTGCTGTGTTTTACTGTCAGCAATATGGTAGTTCA8420.1061975476186272No Hit
CAGTTAGGACCCAGAGGGAACCATGGAAACCCCAGCGCAGCTTCTCTTCCTCCTGCTACTCTGGCTCCCAGATAC8150.10279216307503701No Hit
ATTCAGGGGTGGGGACAGGCGGGCGGCTCAGTAGCAGGTGCCGTCCACCT8070.10178316024730659No Hit
GTTGAAGCCTTCGGAGACCCTGTCCCTCAGCTGCGTTGTGTCTGGTGGGT8070.10178316024730659No Hit
CTCTGGGATAGAAGTTATTCAGCAGGCACACAACAGAGGCAGTTCCAGAT8030.10127865883344138No Hit
ATGCAGGAGAAGGTGTCCCCCTTCTTCCAGTCCTCGGCTGCCACGCGCAG7990.10077415741957615No Hit

[OK]Adapter Content

Adapter graph

[FAIL]Kmer Content

Kmer graph

SequenceCountPValueObs/Exp MaxMax Obs/Exp Position
TCGTATG2200.054.92504540
CGTACTA2300.053.35433629
CGTATGC2400.051.84959441
GGTATCA5600.050.986091
CTAGATC2150.050.88940433
ACCGTAC2450.050.03709827
GAGACCG2450.049.96446624
GACCGTA2500.049.00228526
ATCTCGT2250.048.86953737
GTACTAG2550.048.15398430
TAGATCT2300.047.62795334
ATCTCCG2600.047.0492410
ATGCCGT2700.046.29344644
TATACAC2650.046.1615223
AGACCGT2700.045.35816625
GTATGCC2750.045.29995742
CCGTCTT2800.044.8365747
ACGAGAC2750.044.50537522
CACGAGA2750.044.50256321
ACTAGAT2700.044.29116432
Produced by FastQC (version 0.11.5)