FastQCFastQC Report
Fri 2 Sep 2022
EGAF00004684456

Summary

[OK]Basic Statistics

MeasureValue
FilenameEGAF00004684456
File typeConventional base calls
EncodingSanger / Illumina 1.9
Total Sequences919125
Sequences flagged as poor quality0
Sequence length20-76
%GC54

[OK]Per base sequence quality

Per base quality graph

[OK]Per tile sequence quality

Per tile quality graph

[OK]Per sequence quality scores

Per Sequence quality graph

[FAIL]Per base sequence content

Per base sequence content

[FAIL]Per sequence GC content

Per sequence GC content graph

[OK]Per base N content

N content graph

[WARN]Sequence Length Distribution

Sequence length distribution

[FAIL]Sequence Duplication Levels

Duplication level graph

[WARN]Overrepresented sequences

SequenceCountPercentagePossible Source
CTCCTGGGAGTTACCCGATTGGAGGGCGTTATCCACCTTCCACTGTACTTTGGCCTCTCTGGGATAGAAGTTATT29900.3253093975248198No Hit
CTCTAACACTCTCCCCTGTTGAAGCTCTTTGTGACGGGCGAGCTCAGGCC26950.2932136542907657No Hit
GTAATACACGGCCGTGTCTGCGGCGGTCACAGAGCTCAACTTCAGGGAGA22390.2436012511899905No Hit
GGCGTAGACTTTGTGTTTCTCGTAGTCTGCTTTGCTCAGCGTCAGGGTGC21010.22858697130422956No Hit
CTTTTGCCCCGGGGACCAAGCTGGAAATCAGACGAACTGTGGCTGCACCA20420.22216782265741872No Hit
GTCTCAGTCAGGACACAGCATGGACATGAGGGTCCCCGCTCAGCTCCTGG20420.22216782265741872No Hit
CTTATACACATCTCCGAGCCCACGAGACAAGAGGCAATCTCGTATGCCGT19600.2132462940296478No Hit
GGCTGGAACTGAGGAGCAGGTGGGGGCACTTCTCCCTCTAACACTCTCCC18710.20356317149462805No Hit
GGGCAAAAGTGTGCAGAGAGCTGTAACTCTCTTGACAGTAGTAAGTTGCA18570.20203998368013057No Hit
CAACAGAGGCAGTTCCAGATTTCAACTGCTCATCAGATGGCGGGAAGATG18180.197796817625459No Hit
CAATAGGGGTAGGTCCCCTGTGGAAAAAGGGTCAGAGGCCAAAGGATGGGAGGGGGTCAGGCTGGAACTGAGGAG17380.18909288725690196No Hit
CTCCCAGGAGAGTGTCACAGAGCAGGACAGCAAGGACAGCACCTACAGCC17100.18604651162790697No Hit
GTAGGAGACAGAGTCACCATCACTTGCCGGGCAAGTCAGACCATTAGTACCTATTTACATTGGTATCAGCAACAA16550.18006255949952402No Hit
GTCCTGCTCTGTGACACTCTCCTGGGAGTTACCCGATTGGAGGGCGTTATCCACCTTCCACTGTACTTTGGCCT16470.1791921664626683No Hit
GTCTACGCCTGCGAAGTCACCCATCAGGGCCTGAGCTCGCCCGTCACAAA15910.17309941520467836No Hit
GCGTCAGGGTGCTGCTGAGGCTGTAGGTGCTGTCCTTGCTGTCCTGCTCT15680.17059703522371822No Hit
GGATGGGAGGGGGTCAGGCTGGAACTGAGGAGCAGGTGGGGGCACTTCTCCCTCTAACACTCTCCCCTGTTGAAG14580.15862913096695225No Hit
GTCTCCATCCTCCCTGTCTGCATCTGTAGGAGACAGAGTCACCATCACTTGCCGGGCAAGTCAGACCATTAGTAC14400.15667074663402691No Hit
GCATGGACATGAGGGTCCCCGCTCAGCTCCTGGGGCTCCTGCTACTCTGG14370.15634434924520604No Hit
GTATTACAGTGGGAGTACCAATTACAACCCCTCCCTCAAGAGTCGAGTCACCATTTCAGTAGACACGTCCAAGAA13720.14927240582075343No Hit
CTCTGGGATAGAAGTTATTCAGCAGGCACACAACAGAGGCAGTTCCAGAT13490.1467700258397933No Hit
GTACAGTGGAAGGTGGATAACGCCCTCCAATCGGGTAACTCCCAGGAGAG13260.14426764585883314No Hit
GTCTTCATCTTCCCGCCATCTGATGAGCAGTTGAAATCTGGAACTGCCTCTGTTGTGTGCCTGCTGAATAACTT13230.14394124847001225No Hit
CTGCTACTCTGGCTCCGAGGTGCCAGATGTGACATCCAGATGACCCAGTC13130.14285325717394262No Hit
CCACTGTACTTTGGCCTCTCTGGGATAGAAGTTATTCAGCAGGCACACAACAGAGGCAGTTCCAGATTTCAACTG12700.1381748946008432No Hit
GTAGTAAGTTGCAAAATCTTCAGGTTGCAGACTGGTGATGGTGAGAGTGA12390.13480212158302735No Hit
GTCACATCCTGGCTGGGATTCGTGTAGTGCTTCACGTGGCATGTCACGGA12190.13262613899088807No Hit
GATGAAGACAGATGGTGCAGCCACAGTTCGTCTGATTTCCAGCTTGGTCCCCGGGGCAAAAGTGTGCAGAGAG12170.13240854073167416No Hit
GTGCAGCCACAGTTCGTCTGATTTCCAGCTTGGTCCCCGGGGCAAAAGTG11970.13023255813953488No Hit
GTGCAGTGACAGTCGGGGGTGGCAGCATGAGGGAGATGGGGTAGGTGGAG11890.12936216510267917No Hit
CACCATCACCAGTCTGCAACCTGAAGATTTTGCAACTTACTACTGTCAAG11740.12773017815857474No Hit
GGACACAGCATGGACATGAGGGTCCCCGCTCAGCTCCTGGGGCTCCTGCT11540.12555419556643546No Hit
AATCTATGGTGCATCCACTTTGCAAAGTGGGGTCCCATCAAGGTTCACTGGCAGTGGATCTGGGACAGATTTCA11350.12348701210390317No Hit
CAGTAATACACGGCCGTGTCTGCGGCGGTCACAGAGCTCAACTTCAGGGAGAACTGGTTCTTGGACGTGTCTA11290.1228342173262614No Hit
GGCTGTAGGTGCTGTCCTTGCTGTCCTGCTCTGTGACACTCTCCTGGGAGTTACCCGATTGGAGGGCGTTATCCA11270.12261661906704747No Hit
CAGCATGGACATGAGGGTCCCCGCTCAGCTCCTGGGGCTCCTGCTACTCTGGCTCCGAGGTGCCAGATGTGACAT11200.12185502515979871No Hit
ACTTACTACTGTCAAGAGAGTTACAGCTCTCTGCACACTTTTGCCCCGGGGACCAAGCTGGAAATCAGACGAA11170.12152862777097782No Hit
GTGTATTACTGTGCGAGACGGGGACGCGGAGGGTTTAATTACTTCTTTGA10990.11957024343805249No Hit
GGCCAAAGGATGGGAGGGGGTCAGGCTGGAACTGAGGAGCAGGTGGGGGCACTTCTCCCTCTAACACTCTCCC10880.1183734530123759No Hit
GTATGCTGGTCACAGCGAAGGTGGTGGTGCCCTGGCTGGGCTCCTGCCGG10860.11815585475316197No Hit
GTGGCTGCACCATCTGTCTTCATCTTCCCGCCATCTGATGAGCAGTTGAAATCTGGAACTGCCTCTGTTGTGTGC10830.11782945736434108No Hit
ATCCCAGAGAGGCCAAAGTACAGTGGAAGGTGGATAACGCCCTCCAATCG10770.11717666258669932No Hit
GTGCATCCACTTTGCAAAGTGGGGTCCCATCAAGGTTCACTGGCAGTGGA10590.11521827825377398No Hit
GTCCTGGACCTCCTGTGCAAGAACATGAAACATCTGTGGTTCTTCCTTCTCCTGGTGGCAGCTCCCAGATGGGTC10530.1145654834761322No Hit
GCTCAGTAGCAGGTGCCGTCCACCTCCGCCATGACAACAGACACATTGAC10460.11380388956888346No Hit
GTGTACAGGTCCCCGGAGGCATCCTGGCTGGGTGGGAAGTTTCTGGCGGT10450.1136950904392765No Hit
GTGGATGCACCATAGATTAGGAGCTTAGGGGCTCTCCCTGGTTGTTGCTG10370.11282469740242076No Hit
GAGCTGTAACTCTCTTGACAGTAGTAAGTTGCAAAATCTTCAGGTTGCAGACTGGTGATGGTGAGAGTGAAAT10350.11260709914320685No Hit
TCCTTGGGGCTGAAGCCACGTGCCAGGCACGTCAGCGTCACCAGCTCGTT10300.11206310349517203No Hit
AGGTCACACTGAGTGGCTCCTGGGGGAAGAAGCCCTGGACCAGGCAGGCG10240.11141030871753026No Hit
GTCACTGCACCGACCGGCCCTCGAGGACCTGCTCTTAGGTTCAGAAGCGAACCTCACGTGCACACTGACCGGC10120.1101047191622467No Hit
GGCCCTGATGGGTGACTTCGCAGGCGTAGACTTTGTGTTTCTCGTAGTCTGCTTTGCTCAGCGTCAGGGTGCTG10060.10945192438460492No Hit
GAGCAGGACAGCAAGGACAGCACCTACAGCCTCAGCAGCACCCTGACGCT10000.10879912960696314No Hit
ATGTATTACAGTGGGAGTACCAATTACAACCCCTCCCTCAAGAGTCGAGT9990.10869033047735618No Hit
GCTCCTGGGGCTCCTGCTACTCTGGCTCCGAGGTGCCAGATGTGACATCCAGATGACCCAGTCTCCATCCTCC9940.10814633482932137No Hit
GCTTTGCTCAGCGTCAGGGTGCTGCTGAGGCTGTAGGTGCTGTCCTTGCT9860.10727594179246566No Hit
GATTTCACTCTCACCATCACCAGTCTGCAACCTGAAGATTTTGCAACTTACTACTGTCAAGAGAGTTACAGCTCT9710.10564395484836123No Hit
GTTGTGTGCCTGCTGAATAACTTCTATCCCAGAGAGGCCAAAGTACAGTG9670.10520875832993337No Hit
GAGTTTATTCAGGGGTGGGGACAGGCGGGCGGCTCAGTAGCAGGTGCCGT9650.10499116007071944No Hit
CCATAGATTAGGAGCTTAGGGGCTCTCCCTGGTTGTTGCTGATACCAATG9620.10466476268189856No Hit
GTCCTTGCTGTCCTGCTCTGTGACACTCTCCTGGGAGTTACCCGATTGGAGGGCGTTATCCACCTTCCACTGTA9510.10346797225622194No Hit
ACCAGGGAGAGCCCCTAAGCTCCTAATCTATGGTGCATCCACTTTGCAAAGTGGGGTCCCATCAAGGTTCACTGG9400.10227118183054534No Hit
GTCTGCATCTGTAGGAGACAGAGTCACCATCACTTGCCGGGCAAGTCAGA9320.10140078879368965No Hit
GTATTACTGTGCGAGACGGGGACGCGGAGGGTTTAATTACTTCTTTGACTACTGGGGCCAGGGAACCCTGGTCAC9200.1000951992384061No Hit

[OK]Adapter Content

Adapter graph

Produced by FastQC (version 0.11.9)