FastQCFastQC Report
Sat 23 Apr 2022
EGAF00004684457

Summary

[OK]Basic Statistics

MeasureValue
FilenameEGAF00004684457
File typeConventional base calls
EncodingSanger / Illumina 1.9
Total Sequences919125
Sequences flagged as poor quality0
Sequence length20-76
%GC54

[OK]Per base sequence quality

Per base quality graph

[OK]Per tile sequence quality

Per base quality graph

[OK]Per sequence quality scores

Per Sequence quality graph

[FAIL]Per base sequence content

Per base sequence content

[FAIL]Per sequence GC content

Per sequence GC content graph

[OK]Per base N content

N content graph

[WARN]Sequence Length Distribution

Sequence length distribution

[FAIL]Sequence Duplication Levels

Duplication level graph

[WARN]Overrepresented sequences

SequenceCountPercentagePossible Source
GGGGGGGGGGGGGGGGGGGGGGGGGGGGGGGGGGGGGGGGGGGGGGGGGG38820.42235822113423094No Hit
CTCCTGGGAGTTACCCGATTGGAGGGCGTTATCCACCTTCCACTGTACTTTGGCCTCTCTGGGATAGAAGTTATT29250.3182374541003672No Hit
CTCTAACACTCTCCCCTGTTGAAGCTCTTTGTGACGGGCGAGCTCAGGCC22100.24044607643138854No Hit
GTAATACACGGCCGTGTCTGCGGCGGTCACAGAGCTCAACTTCAGGGAGA19960.21716306269549843No Hit
GTCTCAGTCAGGACACAGCATGGACATGAGGGTCCCCGCTCAGCTCCTGG19940.2169454644362845No Hit
CTTTTGCCCCGGGGACCAAGCTGGAAATCAGACGAACTGTGGCTGCACCA19660.21389908880728956No Hit
GGCGTAGACTTTGTGTTTCTCGTAGTCTGCTTTGCTCAGCGTCAGGGTGC19380.2108527131782946No Hit
CTCCCAGGAGAGTGTCACAGAGCAGGACAGCAAGGACAGCACCTACAGCC18650.20291037671698628No Hit
GGGCAAAAGTGTGCAGAGAGCTGTAACTCTCTTGACAGTAGTAAGTTGCA16700.18169454644362845No Hit
GGCTGGAACTGAGGAGCAGGTGGGGGCACTTCTCCCTCTAACACTCTCCC16170.1759281925744594No Hit
GTCCTGCTCTGTGACACTCTCCTGGGAGTTACCCGATTGGAGGGCGTTATCCACCTTCCACTGTACTTTGGCCT15920.17320821433428532No Hit
CAACAGAGGCAGTTCCAGATTTCAACTGCTCATCAGATGGCGGGAAGATG15370.16722426220590234No Hit
GTAGGAGACAGAGTCACCATCACTTGCCGGGCAAGTCAGACCATTAGTACCTATTTACATTGGTATCAGCAACAA15160.16493948048415613No Hit
GTCTACGCCTGCGAAGTCACCCATCAGGGCCTGAGCTCGCCCGTCACAAA14820.16124031007751938No Hit
GCGTCAGGGTGCTGCTGAGGCTGTAGGTGCTGTCCTTGCTGTCCTGCTCT14060.1529715762273902No Hit
GTCTCCATCCTCCCTGTCTGCATCTGTAGGAGACAGAGTCACCATCACTTGCCGGGCAAGTCAGACCATTAGTAC13990.15220998232014143No Hit
GTATTACAGTGGGAGTACCAATTACAACCCCTCCCTCAAGAGTCGAGTCACCATTTCAGTAGACACGTCCAAGAA13400.14579083367333062No Hit
GCATGGACATGAGGGTCCCCGCTCAGCTCCTGGGGCTCCTGCTACTCTGG13000.1414388684890521No Hit
CTCTGGGATAGAAGTTATTCAGCAGGCACACAACAGAGGCAGTTCCAGAT12960.14100367197062424No Hit
GTCTTCATCTTCCCGCCATCTGATGAGCAGTTGAAATCTGGAACTGCCTCTGTTGTGTGCCTGCTGAATAACTT12920.1405684754521964No Hit
GGATGGGAGGGGGTCAGGCTGGAACTGAGGAGCAGGTGGGGGCACTTCTCCCTCTAACACTCTCCCCTGTTGAAG12760.13882768937848497No Hit
GTACAGTGGAAGGTGGATAACGCCCTCCAATCGGGTAACTCCCAGGAGAG12700.1381748946008432No Hit
CACCATCACCAGTCTGCAACCTGAAGATTTTGCAACTTACTACTGTCAAG12630.13741330069359445No Hit
GTAGTAAGTTGCAAAATCTTCAGGTTGCAGACTGGTGATGGTGAGAGTGA12150.13219094247246022No Hit
AATCTATGGTGCATCCACTTTGCAAAGTGGGGTCCCATCAAGGTTCACTGGCAGTGGATCTGGGACAGATTTCA12070.1313205494356045No Hit
GAGCAGGACAGCAAGGACAGCACCTACAGCCTCAGCAGCACCCTGACGCT11890.12936216510267917No Hit
CAATAGGGGTAGGTCCCCTGTGGAAAAAGGGTCAGAGGCCAAAGGATGGGAGGGGGTCAGGCTGGAACTGAGGAG11760.12794777641778865No Hit
GATGAAGACAGATGGTGCAGCCACAGTTCGTCTGATTTCCAGCTTGGTCCCCGGGGCAAAAGTGTGCAGAGAG11530.1254453964368285No Hit
ACTTACTACTGTCAAGAGAGTTACAGCTCTCTGCACACTTTTGCCCCGGGGACCAAGCTGGAAATCAGACGAA11440.12446620427036584No Hit
GGCTGTAGGTGCTGTCCTTGCTGTCCTGCTCTGTGACACTCTCCTGGGAGTTACCCGATTGGAGGGCGTTATCCA11390.12392220862233103No Hit
CTGCTACTCTGGCTCCGAGGTGCCAGATGTGACATCCAGATGACCCAGTC11370.1237046103631171No Hit
GTGGCTGCACCATCTGTCTTCATCTTCCCGCCATCTGATGAGCAGTTGAAATCTGGAACTGCCTCTGTTGTGTGC11170.12152862777097782No Hit
CAGTAATACACGGCCGTGTCTGCGGCGGTCACAGAGCTCAACTTCAGGGAGAACTGGTTCTTGGACGTGTCTA11120.12098463212294301No Hit
GGACACAGCATGGACATGAGGGTCCCCGCTCAGCTCCTGGGGCTCCTGCT11080.12054943560451516No Hit
CAGCATGGACATGAGGGTCCCCGCTCAGCTCCTGGGGCTCCTGCTACTCTGGCTCCGAGGTGCCAGATGTGACAT11020.11989664082687337No Hit
GTGTATTACTGTGCGAGACGGGGACGCGGAGGGTTTAATTACTTCTTTGA10960.1192438460492316No Hit
GTGCAGCCACAGTTCGTCTGATTTCCAGCTTGGTCCCCGGGGCAAAAGTG10900.11859105127158982No Hit
CCACTGTACTTTGGCCTCTCTGGGATAGAAGTTATTCAGCAGGCACACAACAGAGGCAGTTCCAGATTTCAACTG10850.11804705562355501No Hit
GTTCAAGGACCACCTGAGCGTGACCTCTGTGGCTGCTACAGCGTGTCCAG10600.11532707738338094No Hit
GTGCATCCACTTTGCAAAGTGGGGTCCCATCAAGGTTCACTGGCAGTGGA10250.11151910784713721No Hit
CCCTGACGCTGAGCAAAGCAGACTACGAGAAACACAAAGTCTACGCCTGCGAAGTCACCCATCAGGGCCTGAGCT10230.11130150958792329No Hit
GTCCTGGACCTCCTGTGCAAGAACATGAAACATCTGTGGTTCTTCCTTCTCCTGGTGGCAGCTCCCAGATGGGTC10230.11130150958792329No Hit
ATCCCAGAGAGGCCAAAGTACAGTGGAAGGTGGATAACGCCCTCCAATCG10070.10956072351421188No Hit
GTCACATCCTGGCTGGGATTCGTGTAGTGCTTCACGTGGCATGTCACGGA9910.10781993744050047No Hit
GTGTGACCTGGAGCGAAAGCGGACAGGGCGTGACCGCCAGAAACTTCCCACCCAGCCAGGATGCCTCCGGGGAC9880.10749354005167959No Hit
GTACTTTGGCCTCTCTGGGATAGAAGTTATTCAGCAGGCACACAACAGAGGCAGTTCCAGATTTCAACTGCTCAT9710.10564395484836123No Hit
GTGGATGCACCATAGATTAGGAGCTTAGGGGCTCTCCCTGGTTGTTGCTG9650.10499116007071944No Hit
GTGTACAGGTCCCCGGAGGCATCCTGGCTGGGTGGGAAGTTTCTGGCGGT9570.10412076703386372No Hit
ACCAGGGAGAGCCCCTAAGCTCCTAATCTATGGTGCATCCACTTTGCAAAGTGGGGTCCCATCAAGGTTCACTGG9570.10412076703386372No Hit
GTCCTTGCTGTCCTGCTCTGTGACACTCTCCTGGGAGTTACCCGATTGGAGGGCGTTATCCACCTTCCACTGTA9550.10390316877464979No Hit
GTCTGCATCTGTAGGAGACAGAGTCACCATCACTTGCCGGGCAAGTCAGA9500.10335917312661498No Hit
GTTGTGTGCCTGCTGAATAACTTCTATCCCAGAGAGGCCAAAGTACAGTG9450.10281517747858017No Hit
CCATAGATTAGGAGCTTAGGGGCTCTCCCTGGTTGTTGCTGATACCAATG9240.10053039575683394No Hit
GCTCCTGGGGCTCCTGCTACTCTGGCTCCGAGGTGCCAGATGTGACATCCAGATGACCCAGTCTCCATCCTCC9230.10042159662722698No Hit
GAGCTGTAACTCTCTTGACAGTAGTAAGTTGCAAAATCTTCAGGTTGCAGACTGGTGATGGTGAGAGTGAAAT9220.10031279749762002No Hit

[OK]Adapter Content

Adapter graph

[FAIL]Kmer Content

Kmer graph

SequenceCountPValueObs/Exp MaxMax Obs/Exp Position
GTATCAA21950.038.81361
TACTGAA8150.038.56582670
GTGGTAT2350.036.1091161
GACAGGT2100.035.5588841
ATCAACG27450.030.8794563
TATCAAC27750.030.6744792
TCAACGC27900.030.3814014
CAACGCA28050.030.3398085
AACGCAG28250.030.2483236
GTCGTGC450.005029559630.1416288
TTATACC450.005032236730.1383484
ACGCAGA29400.029.1804777
CGCAGAG30050.028.5492868
AGAGTAC30350.027.93190611
GCAGAGT30700.027.613469
GTCCGTC500.00784263127.52126538
AATGGTA2955.4569682E-1126.63656670
GATACTG659.573877E-426.0841039
CAGAGTA34100.025.05909310
GGTTGAC700.001473849924.2235872
Produced by FastQC (version 0.11.5)