FastQCFastQC Report
Wed 27 Apr 2022
EGAF00004684458

Summary

[OK]Basic Statistics

MeasureValue
FilenameEGAF00004684458
File typeConventional base calls
EncodingSanger / Illumina 1.9
Total Sequences823859
Sequences flagged as poor quality0
Sequence length20-76
%GC56

[OK]Per base sequence quality

Per base quality graph

[OK]Per tile sequence quality

Per tile quality graph

[OK]Per sequence quality scores

Per Sequence quality graph

[FAIL]Per base sequence content

Per base sequence content

[FAIL]Per sequence GC content

Per sequence GC content graph

[OK]Per base N content

N content graph

[WARN]Sequence Length Distribution

Sequence length distribution

[FAIL]Sequence Duplication Levels

Duplication level graph

[WARN]Overrepresented sequences

SequenceCountPercentagePossible Source
GCCCAAGGCTGCCCCCTCGGTCACTCTCTTCCCGCCCTCCTCTGAGGAGC37450.4545680753624103No Hit
CCTATGAGGTGACTCAGCCGCTCTCAGTGTCAGTGGCCCTGGGACAGACG30020.3643827402504555No Hit
GCCTTGGGCTGACCTAGGACGGTCAGCCTGGTCCCTCCGCCGAAAAGCAC28980.35175922093465023No Hit
CTTTACAGGTTCTGTGGCCTCCTATGAGGTGACTCAGCCGCTCTCAGTGT28610.34726816117806564No Hit
GGATTACCTGTGGGGGCAATGACATTGGAGATAAAAATGTGCACTGGTACCAGCAGAAGCCAGGCCAGGCCC26050.31619488286223735No Hit
GACCTAGGACGGTCAGCCTGGTCCCTCCGCCGAAAAGCACAGAGGCAGTG24940.3027217035924836No Hit
CCTCTGAGGAGCTTCAAGCCAACAAGGCCACACTGGTGTGTCTCATAAGT24440.29665270392142346No Hit
GGCCTGGACCGCTCTCCTTCTGAGCCTCCTTGCTCACTTTACAGGTTCTG23700.2876705844082543No Hit
GTGTGGTGGTCTCCACTCCCGCCTTGACGGGGCTGCTATCTGCCTTCCAG23250.2822084847043001No Hit
GCACAGGGGCCTGGCCTGGCTTCTGCTGGTACCAGTGCACATTTTTATCT21710.26351596571743463No Hit
CCCCTGTGCTGGTCATTCATAGGGACAGCAGCCGGCCCTCTGGGATCCCT21620.2624235457766438No Hit
GACTATTACTGTCAAATATGGTACGTCAACACTGCCTCTGTGCTTTTCGG20130.24433792675688437No Hit
GTAATAGTCAGCCTCATCCCCGGCTTGGGCTCTAGTGATGGTCAGGGTGG19550.2372978871384545No Hit
CCTCAGAGGAGGGCGGGAAGAGAGTGACCGAGGGGGCAGCCTTGGGCTGACCTAGGACGGTCAGCCTGGTCCCTC19430.23584132721740006No Hit
TCCTAGGTCAGCCCAAGGCTGCCCCCTCGGTCACTCTCTTCCCGCCCTCCTCTGAGGAGCTTCAAGCCAACAAGG18440.22382470786870085No Hit
GTCCTAGGTCAGCCCAAGGCTGCCCCCTCGGTCACTCTCTTCCCGCCCTC18410.2234605678884372No Hit
CCTATGAACATTCTGTAGGGGCCACTGTCTTCTCCACGGTGCTCCCTTCATGCGTGACCTGGCAGCTGTAGCTT18060.21921226811869504No Hit
GCCATGGCCTGGACCGCTCTCCTTCTGAGCCTCCTTGCTCACTTTACAGGTTCTGTGGCCTCCTATGAGGTGACT17750.21544948832263772No Hit
GTGCAGGGAGAAGGGCTTGATGCCTTGGGGTGGGAGGAGAGACCCCTCCCCTGGGATCCTGCAGCTCTAGTCTCC17650.2142356883884257No Hit
GTCTTCTCCACGGTGCTCCCTTCATGCGTGACCTGGCAGCTGTAGCTTCT17070.20719564876999583No Hit
GGGTTTATTGAGTGCAGGGAGAAGGGCTTGATGCCTTGGGGTGGGAGGAGAGACCCCTCCCCTGGGATCCTGCAG16540.200762509118672No Hit
GTGCTCCCTTCATGCGTGACCTGGCAGCTGTAGCTTCTGTGGGACTTCCACTGCTCAGGCGTCAGGCTCAGGTAG16120.1956645493949814No Hit
TTCATAGGGACAGCAGCCGGCCCTCTGGGATCCCTGACCGATTCTCTGGCTCCAACTCGGGGAATATGGCCACC15830.1921445295857665No Hit
GTGTTGACGTACCATATTTGACAGTAATAGTCAGCCTCATCCCCGGCTTG15360.18643966989496988No Hit
GTTTGGAGGGTGTGGTGGTCTCCACTCCCGCCTTGACGGGGCTGCTATCTGCCTTCCAGGCCACTGTCACGGCTC15270.18534724995417903No Hit
CTCTCTTCCCGCCCTCCTCTGAGGAGCTTCAAGCCAACAAGGCCACACTGGTGTGTCTCATAAGTGACTTCTACC15220.18474034998707303No Hit
GCTCAGGCGTCAGGCTCAGGTAGCTGCTGGCCGCGTACTTGTTGTTGCTTTGTTTGGAGGGTGTGGTGGTCTCCA14730.17879273030943402No Hit
CACCAGTGTGGCCTTGTTGGCTTGAAGCTCCTCAGAGGAGGGCGGGAAGAGAGTGACCGAGGGGGCAGCCTTGGG14220.17260235064495258No Hit
GGTTTATTGAGTGCAGGGAGAAGGGCTTGATGCCTTGGGGTGGGAGGAGAGACCCCTCCCCTGGGATCCTGCAG14040.17041751076337092No Hit
GAGAAGGGCTTGATGCCTTGGGGTGGGAGGAGAGACCCCTCCCCTGGGAT13950.1693250908225801No Hit
CCACCACACCCTCCAAACAAAGCAACAACAAGTACGCGGCCAGCAGCTAC13440.16313471115809866No Hit
CTTATGAGACACACCAGTGTGGCCTTGTTGGCTTGAAGCTCCTCAGAGGA13070.1586436514015141No Hit
GGCTCAGGTAGCTGCTGGCCGCGTACTTGTTGTTGCTTTGTTTGGAGGGTGTGGTGGTCTCCACTCCCGCCTTGA12930.15694433149361725No Hit
ACACTGGTGTGTCTCATAAGTGACTTCTACCCGGGAGCCGTGACAGTGGC12790.15524501158572038No Hit
CTTCATGCGTGACCTGGCAGCTGTAGCTTCTGTGGGACTTCCACTGCTCAGGCGTCAGGCTCAGGTAGCTGCTGG12780.15512363159229917No Hit
CCCTGGGACAGACGGCCAGGATTACCTGTGGGGGCAATGACATTGGAGAT12660.15366707167124471No Hit
ACCTAGGACGGTCAGCCTGGTCCCTCCGCCGAAAAGCACAGAGGCAGTGT12290.14917601191466015No Hit
GCTATCTGCCTTCCAGGCCACTGTCACGGCTCCCGGGTAGAAGTCACTTA12160.14759807200018452No Hit
CCCTTCATGCGTGACCTGGCAGCTGTAGCTTCTGTGGGACTTCCACTGCTCAGGCGTCAGGCTCAGGTAGCTG12150.1474766920067633No Hit
GGCCAGGCCCCTGTGCTGGTCATTCATAGGGACAGCAGCCGGCCCTCTGG12130.14723393201992088No Hit
GCATCGGGGGTGCCGCAGCCATGGCCTGGACCGCTCTCCTTCTGAGCCTCCTTGCTCACTTTACAGGTTCTGTGG12070.14650565205939367No Hit
GTGCTGGTCATTCATAGGGACAGCAGCCGGCCCTCTGGGATCCCTGACCG11900.1444421921712332No Hit
CTCTCCTTCTGAGCCTCCTTGCTCACTTTACAGGTTCTGTGGCCTCCTATGAGGTGACTCAGCCGCTCTCAGTGT11690.1418932123093879No Hit
GCCTTGGGGTGGGAGGAGAGACCCCTCCCCTGGGATCCTGCAGCTCTAGT11490.13946561244096378No Hit
GCTCTAGTCTCCCGTGGTGGGGGTGAGGGTTGAGAACCTATGAACATTCT11430.13873733248043657No Hit
GACCATCACTAGAGCCCAAGCCGGGGATGAGGCTGACTATTACTGTCAAA11240.1364311126054337No Hit
CTTATACACATCTCCGAGCCCACGAGACTGCAGCTAATCTCGTATGCCGT11110.13485317269095803No Hit
GGTGTGTCTCATAAGTGACTTCTACCCGGGAGCCGTGACAGTGGCCTGGA11070.13436765271727322No Hit
GAGTACGGGAGCCTGGGCCTCAGGAAGCAGCATCGGGGGTGCCGCAGCCATGGCCTGGACCGCTCTCCTTCTGAG11030.1338821327435884No Hit
CCTTCTGAGCCTCCTTGCTCACTTTACAGGTTCTGTGGCCTCCTATGAGG10970.1331538527830612No Hit
TCACTAGAGCCCAAGCCGGGGATGAGGCTGACTATTACTGTCAAATATGG10930.13266833280937637No Hit
GCCTGGTCCCTCCGCCGAAAAGCACAGAGGCAGTGTTGACGTACCATATTTGACAGTAATAGTCAGCCTCATCCC10760.1306048729212159No Hit
CGTCAACACTGCCTCTGTGCTTTTCGGCGGAGGGACCAGGCTGACCGTCC10660.12939107298700384No Hit
GTACGGGAGCCTGGGCCTCAGGAAGCAGCATCGGGGGTGCCGCAGCCATGGCCTGGACCGCTCTCCTTCTGAGC10390.12611381316463133No Hit
GTCTCTGGTGGCTCCATCAGTCCTTACTACTGGAGCTGGATCCGGCAGCC10210.12392897328304965No Hit
CCCCTGGGATCCTGCAGCTCTAGTCTCCCGTGGTGGGGGTGAGGGTTGAG10080.122351033368574No Hit
CAGGTAATCCTGGCCGTCTGTCCCAGGGCCACTGACACTGAGAGCGGCTGAGTCACCTCATAGGAGGCCACAGAA10050.12198689338831037No Hit
CCTCATAGGAGGCCACAGAACCTGTAAAGTGAGCAAGGAGGCTCAGAAGGAGAGCGGTCCAGGCCATGGCTGCGG9990.12125861342778316No Hit
GAATATGGCCACCCTGACCATCACTAGAGCCCAAGCCGGGGATGAGGCTGACTATTACTGTCAAATATGGTACGT9890.1200448134935711No Hit
ACCTGAGCCTGACGCCTGAGCAGTGGAAGTCCCACAGAAGCTACAGCTGC9850.11955929351988628No Hit
CCCTATGAATGACCAGCACAGGGGCCTGGCCTGGCTTCTGCTGGTACCAG9780.11870963356593786No Hit
GGGTGGCCATATTCCCCGAGTTGGAGCCAGAGAATCGGTCAGGGATCCCA9700.11773859361856823No Hit
CCCGTGGTGGGGGTGAGGGTTGAGAACCTATGAACATTCTGTAGGGGCCA9670.11737445363830461No Hit
CTCTCAGTGTCAGTGGCCCTGGGACAGACGGCCAGGATTACCTGTGGGGGCAATGACATTGGAGATAAAAATGTG9660.11725307364488342No Hit
GTATCAACGCAGAGTACGGGAGCCTGGGCCTCAGGAAGCAGCATCGGGGGTGCCGCAGCCATGGCCTGGACCGCT9610.1166461736777774No Hit
GTTGAGAACCTATGAACATTCTGTAGGGGCCACTGTCTTCTCCACGGTGCTCCCTTCATGCGTGACCTGGCAG9610.1166461736777774No Hit
TGGCCACCCTGACCATCACTAGAGCCCAAGCCGGGGATGAGGCTGACTATTACTGTCAAATATGGTACGTCAACA9550.11591789371725016No Hit
GGACCAGGCTGACCGTCCTAGGTCAGCCCAAGGCTGCCCCCTCGGTCACTCTCTTCCCGCCCTCCTCTGAGGAG9550.11591789371725016No Hit
CAGTGTCAGTGGCCCTGGGACAGACGGCCAGGATTACCTGTGGGGGCAATGACATTGGAGATAAAAATGTGCA9350.11349029384882607No Hit
GCTGTAGCTTCTGTGGGACTTCCACTGCTCAGGCGTCAGGCTCAGGTAGC9320.11312615386856247No Hit
GGTAGCTGCTGGCCGCGTACTTGTTGTTGCTTTGTTTGGAGGGTGTGGTGGTCTCCACTCCCGCCTTGACGGGG9240.11215511392119282No Hit
GGGCTGCTATCTGCCTTCCAGGCCACTGTCACGGCTCCCGGGTAGAAGTCACTTATGAGACACACCAGTGTGGC9230.11203373392777163No Hit
GTCAAATATGGTACGTCAACACTGCCTCTGTGCTTTTCGGCGGAGGGACC9230.11203373392777163No Hit
CTATTACTGTCAAATATGGTACGTCAACACTGCCTCTGTGCTTTTCGGCG9210.11179097394092921No Hit
GACCAGCACAGGGGCCTGGCCTGGCTTCTGCTGGTACCAGTGCACATTTT9050.10984889404618994No Hit
CTTCCCGCCCTCCTCTGAGGAGCTTCAAGCCAACAAGGCCACACTGGTGT8930.10839233412513549No Hit
GACTTCCACTGCTCAGGCGTCAGGCTCAGGTAGCTGCTGGCCGCGTACTT8850.10742129417776586No Hit
CTGTAGGGGCCACTGTCTTCTCCACGGTGCTCCCTTCATGCGTGACCTGG8690.10547921428302658No Hit
GTGTCAGTGGCCCTGGGACAGACGGCCAGGATTACCTGTGGGGGCAATGA8660.10511507430276297No Hit
CTGGGATCCCTGACCGATTCTCTGGCTCCAACTCGGGGAATATGGCCACC8470.1028088544277601No Hit
CTTCTCCACGGTGCTCCCTTCATGCGTGACCTGGCAGCTGTAGCTTCTGT8460.10268747443433889No Hit
GTGGAAGTCCCACAGAAGCTACAGCTGCCAGGTCACGCATGAAGGGAGCA8430.10232333445407528No Hit

[OK]Adapter Content

Adapter graph

Produced by FastQC (version 0.11.9)