FastQCFastQC Report
Sat 23 Apr 2022
EGAF00004684459

Summary

[OK]Basic Statistics

MeasureValue
FilenameEGAF00004684459
File typeConventional base calls
EncodingSanger / Illumina 1.9
Total Sequences823859
Sequences flagged as poor quality0
Sequence length20-76
%GC57

[OK]Per base sequence quality

Per base quality graph

[OK]Per tile sequence quality

Per base quality graph

[OK]Per sequence quality scores

Per Sequence quality graph

[FAIL]Per base sequence content

Per base sequence content

[FAIL]Per sequence GC content

Per sequence GC content graph

[OK]Per base N content

N content graph

[WARN]Sequence Length Distribution

Sequence length distribution

[FAIL]Sequence Duplication Levels

Duplication level graph

[WARN]Overrepresented sequences

SequenceCountPercentagePossible Source
GCCCAAGGCTGCCCCCTCGGTCACTCTCTTCCCGCCCTCCTCTGAGGAGC34700.42118857717157915No Hit
GCCTTGGGCTGACCTAGGACGGTCAGCCTGGTCCCTCCGCCGAAAAGCAC26990.32760460224383053No Hit
CTTTACAGGTTCTGTGGCCTCCTATGAGGTGACTCAGCCGCTCTCAGTGT26750.32469148240172163No Hit
GGATTACCTGTGGGGGCAATGACATTGGAGATAAAAATGTGCACTGGTACCAGCAGAAGCCAGGCCAGGCCC26110.3169231628227646No Hit
CCTATGAGGTGACTCAGCCGCTCTCAGTGTCAGTGGCCCTGGGACAGACG25950.31498108292802535No Hit
GGGGGGGGGGGGGGGGGGGGGGGGGGGGGGGGGGGGGGGGGGGGGGGGGG25090.30454240349380174No Hit
GTGTGGTGGTCTCCACTCCCGCCTTGACGGGGCTGCTATCTGCCTTCCAG24400.29616718394773867No Hit
CCTCTGAGGAGCTTCAAGCCAACAAGGCCACACTGGTGTGTCTCATAAGT24360.2956816639740538No Hit
GGCCTGGACCGCTCTCCTTCTGAGCCTCCTTGCTCACTTTACAGGTTCTG22700.2755325850661339No Hit
GCACAGGGGCCTGGCCTGGCTTCTGCTGGTACCAGTGCACATTTTTATCT21860.26533666561875274No Hit
CCCCTGTGCTGGTCATTCATAGGGACAGCAGCCGGCCCTCTGGGATCCCT20930.2540483262305807No Hit
GACCTAGGACGGTCAGCCTGGTCCCTCCGCCGAAAAGCACAGAGGCAGTG20610.2501641664411022No Hit
GACTATTACTGTCAAATATGGTACGTCAACACTGCCTCTGTGCTTTTCGG18450.22394608786212206No Hit
GCCATGGCCTGGACCGCTCTCCTTCTGAGCCTCCTTGCTCACTTTACAGGTTCTGTGGCCTCCTATGAGGTGACT18340.2226109079344888No Hit
GTGCAGGGAGAAGGGCTTGATGCCTTGGGGTGGGAGGAGAGACCCCTCCCCTGGGATCCTGCAGCTCTAGTCTCC18060.21921226811869504No Hit
GGGTTTATTGAGTGCAGGGAGAAGGGCTTGATGCCTTGGGGTGGGAGGAGAGACCCCTCCCCTGGGATCCTGCAG17790.21593500829632256No Hit
TCCTAGGTCAGCCCAAGGCTGCCCCCTCGGTCACTCTCTTCCCGCCCTCCTCTGAGGAGCTTCAAGCCAACAAGG17790.21593500829632256No Hit
GTAATAGTCAGCCTCATCCCCGGCTTGGGCTCTAGTGATGGTCAGGGTGG17730.21520672833579532No Hit
CCTCAGAGGAGGGCGGGAAGAGAGTGACCGAGGGGGCAGCCTTGGGCTGACCTAGGACGGTCAGCCTGGTCCCTC17350.21059428858578957No Hit
GTCCTAGGTCAGCCCAAGGCTGCCCCCTCGGTCACTCTCTTCCCGCCCTC17200.20877358868447152No Hit
CCTATGAACATTCTGTAGGGGCCACTGTCTTCTCCACGGTGCTCCCTTCATGCGTGACCTGGCAGCTGTAGCTT17090.20743840875683825No Hit
GTCTTCTCCACGGTGCTCCCTTCATGCGTGACCTGGCAGCTGTAGCTTCT16790.2037970089542021No Hit
GTTTGGAGGGTGTGGTGGTCTCCACTCCCGCCTTGACGGGGCTGCTATCTGCCTTCCAGGCCACTGTCACGGCTC15330.18607552991470627No Hit
TTCATAGGGACAGCAGCCGGCCCTCTGGGATCCCTGACCGATTCTCTGGCTCCAACTCGGGGAATATGGCCACC15240.18498310997391543No Hit
GTGCTCCCTTCATGCGTGACCTGGCAGCTGTAGCTTCTGTGGGACTTCCACTGCTCAGGCGTCAGGCTCAGGTAG15190.1843762100068094No Hit
CTCTCTTCCCGCCCTCCTCTGAGGAGCTTCAAGCCAACAAGGCCACACTGGTGTGTCTCATAAGTGACTTCTACC15180.18425483001338822No Hit
GTGTTGACGTACCATATTTGACAGTAATAGTCAGCCTCATCCCCGGCTTG14560.17672927042127354No Hit
GGTTTATTGAGTGCAGGGAGAAGGGCTTGATGCCTTGGGGTGGGAGGAGAGACCCCTCCCCTGGGATCCTGCAG13530.16422713109888948No Hit
CACCAGTGTGGCCTTGTTGGCTTGAAGCTCCTCAGAGGAGGGCGGGAAGAGAGTGACCGAGGGGGCAGCCTTGGG13330.1617995312304654No Hit
GAGAAGGGCTTGATGCCTTGGGGTGGGAGGAGAGACCCCTCCCCTGGGAT13130.15937193136204134No Hit
CTTCATGCGTGACCTGGCAGCTGTAGCTTCTGTGGGACTTCCACTGCTCAGGCGTCAGGCTCAGGTAGCTGCTGG13080.1587650313949353No Hit
GCTCAGGCGTCAGGCTCAGGTAGCTGCTGGCCGCGTACTTGTTGTTGCTTTGTTTGGAGGGTGTGGTGGTCTCCA12520.15196775176334784No Hit
ACACTGGTGTGTCTCATAAGTGACTTCTACCCGGGAGCCGTGACAGTGGC12220.14832635196071173No Hit
GGGTGGCCATATTCCCCGAGTTGGAGCCAGAGAATCGGTCAGGGATCCCA12170.1477194519936057No Hit
CCCTTCATGCGTGACCTGGCAGCTGTAGCTTCTGTGGGACTTCCACTGCTCAGGCGTCAGGCTCAGGTAGCTG12050.14626289207255125No Hit
CCACCACACCCTCCAAACAAAGCAACAACAAGTACGCGGCCAGCAGCTAC11800.14322839223702113No Hit
GCATCGGGGGTGCCGCAGCCATGGCCTGGACCGCTCTCCTTCTGAGCCTCCTTGCTCACTTTACAGGTTCTGTGG11780.14298563225017874No Hit
GGTGTGTCTCATAAGTGACTTCTACCCGGGAGCCGTGACAGTGGCCTGGA11730.1423787322830727No Hit
CCCTGGGACAGACGGCCAGGATTACCTGTGGGGGCAATGACATTGGAGAT11700.1420145923028091No Hit
CTTATGAGACACACCAGTGTGGCCTTGTTGGCTTGAAGCTCCTCAGAGGA11700.1420145923028091No Hit
GGCTCAGGTAGCTGCTGGCCGCGTACTTGTTGTTGCTTTGTTTGGAGGGTGTGGTGGTCTCCACTCCCGCCTTGA11520.13982975242122742No Hit
CAGGTAATCCTGGCCGTCTGTCCCAGGGCCACTGACACTGAGAGCGGCTGAGTCACCTCATAGGAGGCCACAGAA11240.1364311126054337No Hit
CTCTCCTTCTGAGCCTCCTTGCTCACTTTACAGGTTCTGTGGCCTCCTATGAGGTGACTCAGCCGCTCTCAGTGT11000.1335179927633248No Hit
GCCTTGGGGTGGGAGGAGAGACCCCTCCCCTGGGATCCTGCAGCTCTAGT10970.1331538527830612No Hit
GGCCAGGCCCCTGTGCTGGTCATTCATAGGGACAGCAGCCGGCCCTCTGG10910.13242557282253395No Hit
GCCTGGTCCCTCCGCCGAAAAGCACAGAGGCAGTGTTGACGTACCATATTTGACAGTAATAGTCAGCCTCATCCC10880.13206143284227034No Hit
GACCATCACTAGAGCCCAAGCCGGGGATGAGGCTGACTATTACTGTCAAA10820.1313331528817431No Hit
CCTTCTGAGCCTCCTTGCTCACTTTACAGGTTCTGTGGCCTCCTATGAGG10730.13024073294095226No Hit
GCTCTAGTCTCCCGTGGTGGGGGTGAGGGTTGAGAACCTATGAACATTCT10550.1280558930593706No Hit
GCTATCTGCCTTCCAGGCCACTGTCACGGCTCCCGGGTAGAAGTCACTTA10410.12635657315147375No Hit
GTGCTGGTCATTCATAGGGACAGCAGCCGGCCCTCTGGGATCCCTGACCG10400.12623519315805254No Hit
TCACTAGAGCCCAAGCCGGGGATGAGGCTGACTATTACTGTCAAATATGG10330.12538553320410412No Hit
ACCTAGGACGGTCAGCCTGGTCCCTCCGCCGAAAAGCACAGAGGCAGTGT10290.12490001323041929No Hit
GTACGGGAGCCTGGGCCTCAGGAAGCAGCATCGGGGGTGCCGCAGCCATGGCCTGGACCGCTCTCCTTCTGAGC10260.12453587325015567No Hit
GTCTCTGGTGGCTCCATCAGTCCTTACTACTGGAGCTGGATCCGGCAGCC9980.12113723343436195No Hit
CCTCATAGGAGGCCACAGAACCTGTAAAGTGAGCAAGGAGGCTCAGAAGGAGAGCGGTCCAGGCCATGGCTGCGG9880.11992343350014989No Hit
GAATATGGCCACCCTGACCATCACTAGAGCCCAAGCCGGGGATGAGGCTGACTATTACTGTCAAATATGGTACGT9830.11931653353304389No Hit
GAGTACGGGAGCCTGGGCCTCAGGAAGCAGCATCGGGGGTGCCGCAGCCATGGCCTGGACCGCTCTCCTTCTGAG9820.11919515353962268No Hit
GCTGTAGCTTCTGTGGGACTTCCACTGCTCAGGCGTCAGGCTCAGGTAGC9740.11822411359225304No Hit
GGGCTGCTATCTGCCTTCCAGGCCACTGTCACGGCTCCCGGGTAGAAGTCACTTATGAGACACACCAGTGTGGC9610.1166461736777774No Hit
CGTCAACACTGCCTCTGTGCTTTTCGGCGGAGGGACCAGGCTGACCGTCC9530.11567513373040776No Hit
CTCTCAGTGTCAGTGGCCCTGGGACAGACGGCCAGGATTACCTGTGGGGGCAATGACATTGGAGATAAAAATGTG9470.11494685376988052No Hit
CTGGGATCCCTGACCGATTCTCTGGCTCCAACTCGGGGAATATGGCCACC9440.11458271378961692No Hit
ACCTGAGCCTGACGCCTGAGCAGTGGAAGTCCCACAGAAGCTACAGCTGC9300.11288339388172006No Hit
GGACCAGGCTGACCGTCCTAGGTCAGCCCAAGGCTGCCCCCTCGGTCACTCTCTTCCCGCCCTCCTCTGAGGAG9270.11251925390145645No Hit
CCCTATGAATGACCAGCACAGGGGCCTGGCCTGGCTTCTGCTGGTACCAG9260.11239787390803524No Hit
GGTAGCTGCTGGCCGCGTACTTGTTGTTGCTTTGTTTGGAGGGTGTGGTGGTCTCCACTCCCGCCTTGACGGGG9230.11203373392777163No Hit
TGGCCACCCTGACCATCACTAGAGCCCAAGCCGGGGATGAGGCTGACTATTACTGTCAAATATGGTACGTCAACA9180.1114268339606656No Hit
GTCAAATATGGTACGTCAACACTGCCTCTGTGCTTTTCGGCGGAGGGACC9180.1114268339606656No Hit
CTATTACTGTCAAATATGGTACGTCAACACTGCCTCTGTGCTTTTCGGCG9130.11081993399355958No Hit
GTTGAGAACCTATGAACATTCTGTAGGGGCCACTGTCTTCTCCACGGTGCTCCCTTCATGCGTGACCTGGCAG9090.11033441401987476No Hit
GACCAGCACAGGGGCCTGGCCTGGCTTCTGCTGGTACCAGTGCACATTTT9000.10924199407908392No Hit
CTTCTCCACGGTGCTCCCTTCATGCGTGACCTGGCAGCTGTAGCTTCTGT8910.10814957413829307No Hit
CCCGTGGTGGGGGTGAGGGTTGAGAACCTATGAACATTCTGTAGGGGCCA8910.10814957413829307No Hit
CCCCTGGGATCCTGCAGCTCTAGTCTCCCGTGGTGGGGGTGAGGGTTGAG8870.10766405416460827No Hit
GTGGAAGTCCCACAGAAGCTACAGCTGCCAGGTCACGCATGAAGGGAGCA8770.10645025423039622No Hit
GACTTCCACTGCTCAGGCGTCAGGCTCAGGTAGCTGCTGGCCGCGTACTT8540.10365851438170852No Hit
GTATCAACGCAGAGTACGGGAGCCTGGGCCTCAGGAAGCAGCATCGGGGGTGCCGCAGCCATGGCCTGGACCGCT8520.1034157543948661No Hit
CTTCCCGCCCTCCTCTGAGGAGCTTCAAGCCAACAAGGCCACACTGGTGT8440.10244471444749648No Hit
CAGTGTCAGTGGCCCTGGGACAGACGGCCAGGATTACCTGTGGGGGCAATGACATTGGAGATAAAAATGTGCA8290.10062401454617842No Hit

[OK]Adapter Content

Adapter graph

[WARN]Kmer Content

Kmer graph

SequenceCountPValueObs/Exp MaxMax Obs/Exp Position
TACGTAT350.00446247258.7174670
ACTCGTT200.00553405955.61462464
ACGGTCC200.0077898151.0093335
GGTATCA6150.040.0143851
GTGGTAT2050.039.4662441
GTATCAA14300.035.8324051
TGGTATC2350.035.8254932
ACTGATG5400.030.4460970
ATCAACG17150.029.8432963
TATCAAC17300.029.5881272
GCTTGCC803.3058786E-629.4969041
TCAACGC17850.029.0502474
CAACGCA17950.028.8884075
AACGCAG18450.028.2880276
AGAGTAC18850.027.15185711
TTAAGAC500.00870681826.93754
ACGCAGA19350.026.798297
CGCAGAG19100.026.7964678
CAGAGTA19150.026.72650310
GCAGAGT19500.026.2467969
Produced by FastQC (version 0.11.5)