FastQCFastQC Report
Wed 27 Apr 2022
EGAF00004684460

Summary

[OK]Basic Statistics

MeasureValue
FilenameEGAF00004684460
File typeConventional base calls
EncodingSanger / Illumina 1.9
Total Sequences644918
Sequences flagged as poor quality0
Sequence length20-76
%GC54

[OK]Per base sequence quality

Per base quality graph

[OK]Per tile sequence quality

Per tile quality graph

[OK]Per sequence quality scores

Per Sequence quality graph

[FAIL]Per base sequence content

Per base sequence content

[FAIL]Per sequence GC content

Per sequence GC content graph

[OK]Per base N content

N content graph

[WARN]Sequence Length Distribution

Sequence length distribution

[FAIL]Sequence Duplication Levels

Duplication level graph

[WARN]Overrepresented sequences

SequenceCountPercentagePossible Source
ATATTATAGTGCTCCGTGGACGTTCGGCCAAGGGACCAAGGTGGAAATCAAACGAACTGTGGCTGCACCAT58780.9114337016488918No Hit
CTATAATATTGGTGACAATAATAAACTGCCACATCCTCAGCCTGCAGGCTGCTGATGGTGAGAGTGAAATCTGTC35290.5472013496289451No Hit
CTCTTATACACCTCCGACAACAGGAACTACTTGGCTTGGTTGCAGCAGAA32840.5092120238541954No Hit
CCTTAGGAGGCTGTCCTGGTTTCTGCTGCAACCAAGCCAAGTAGTTCCTG29640.45959331263819586No Hit
CTCCTGGGAGTTACCCGATTGGAGGGCGTTATCCACCTTCCACTGTACTTTGGCCTCTCTGGGATAGAAGTTATT27160.42113881144579624No Hit
CTTATACACATCTCCGAGCCCACGAGACCCTAAGACATCTCGTATGCCGT26530.4113701276751463No Hit
CTCTAACACTCTCCCCTGTTGAAGCTCTTTGTGACGGGCGAGCTCAGGCC26480.4105948353123963No Hit
CTCCTAAGGTGCTCATTTATTGGGCATCCACCCGGGAATCCGGGGTCCCT24510.3800483162200466No Hit
GGCGTAGACTTTGTGTTTCTCGTAGTCTGCTTTGCTCAGCGTCAGGGTGC19120.2964717995155973No Hit
CTTATACACCTCCGACAACAGGAACTACTTGGCTTGGTTGCAGCAGAAAC18820.2918200453390974No Hit
GGCTGGAACTGAGGAGCAGGTGGGGGCACTTCTCCCTCTAACACTCTCCC17480.27104221001739753No Hit
CAACAGAGGCAGTTCCAGATTTCAACTGCTCATCAGATGGCGGGAAGATG16260.2521250763662977No Hit
GTATAAGAGACTCCGGCTGGACTTGCAGTTGATGGTGGCCCGCTCGCCCAGAGACACAGCCAGAGAGTCTGGAGA16090.24948908233294775No Hit
CAATAGGGGTAGGTCCCCTGTGGAAAAAGGGTCAGAGGCCAAAGGATGGGAGGGGGTCAGGCTGGAACTGAGGAG15820.2453025035740978No Hit
GGATGGGAGGGGGTCAGGCTGGAACTGAGGAGCAGGTGGGGGCACTTCTCCCTCTAACACTCTCCCCTGTTGAAG15780.2446822696838978No Hit
GTCCTGCTCTGTGACACTCTCCTGGGAGTTACCCGATTGGAGGGCGTTATCCACCTTCCACTGTACTTTGGCCT15350.23801475536424788No Hit
GTCTACGCCTGCGAAGTCACCCATCAGGGCCTGAGCTCGCCCGTCACAAA14570.22592019450534798No Hit
GCGTCAGGGTGCTGCTGAGGCTGTAGGTGCTGTCCTTGCTGTCCTGCTCT14110.21878750476804804No Hit
CTCCCAGGAGAGTGTCACAGAGCAGGACAGCAAGGACAGCACCTACAGCC13950.21630656920724806No Hit
GTGTTGCAGACCCAGGTCTTCATTTCTCTGTTGCTCTGGATCTCTGGTGC13440.2083985871071981No Hit
GTCTCCAGACTCTCTGGCTGTGTCTCTGGGCGAGCGGGCCACCATCAACTGCAAGTCCAGCCGGAGTCTCTTATA13250.20545247612874817No Hit
GTACAGTGGAAGGTGGATAACGCCCTCCAATCGGGTAACTCCCAGGAGAG13080.20281648209539818No Hit
CCACTGTACTTTGGCCTCTCTGGGATAGAAGTTATTCAGCAGGCACACAACAGAGGCAGTTCCAGATTTCAACTG12420.19258262290709827No Hit
GTCTTCATCTTCCCGCCATCTGATGAGCAGTTGAAATCTGGAACTGCCTCTGTTGTGTGCCTGCTGAATAACTT11830.18343417302664836No Hit
GTCTCTGGGCGAGCGGGCCACCATCAACTGCAAGTCCAGCCGGAGTCTCTTATACACCTCCGACAACAGGAACTA11520.1786273603775984No Hit
GGCCAAAGGATGGGAGGGGGTCAGGCTGGAACTGAGGAGCAGGTGGGGGCACTTCTCCCTCTAACACTCTCCC10920.1693238520245985No Hit
CTCTGGGATAGAAGTTATTCAGCAGGCACACAACAGAGGCAGTTCCAGAT10890.16885867660694848No Hit
AACCAGGACAGCCTCCTAAGGTGCTCATTTATTGGGCATCCACCCGGGAA10820.1677732672990985No Hit
GGCTGTAGGTGCTGTCCTTGCTGTCCTGCTCTGTGACACTCTCCTGGGAGTTACCCGATTGGAGGGCGTTATCCA10630.1648271563206485No Hit
GTGGCTGCACCATCTGTCTTCATCTTCCCGCCATCTGATGAGCAGTTGAAATCTGGAACTGCCTCTGTTGTGTGC10510.16296645465004853No Hit
CTCTGGCTGTGTCTCTGGGCGAGCGGGCCACCATCAACTGCAAGTCCAGC10100.1566090572754986No Hit
CTCTCTGGCTGTGTCTCTGGGCGAGCGGGCCACCATCAACTGCAAGTCCAGCCGGAGTCTCTTATACACCTCCGA9550.14808084128524868No Hit
GTCCCTGACCGATTCAGTGGCAGCGGGTCTGGGACAGATTTCACTCTCAC9490.14715049044994868No Hit
GTATGCTGGTCACAGCGAAGGTGGTGGTGCCCTGGCTGGGCTCCTGCCGG9270.1437392040538487No Hit
TCCTTGGGGCTGAAGCCACGTGCCAGGCACGTCAGCGTCACCAGCTCGTT9170.1421886193283487No Hit
GTGCAGCCACAGTTCGTTTGATTTCCACCTTGGTCCCTTGGCCGAACGTC9130.14156838543814873No Hit
ATCCCAGAGAGGCCAAAGTACAGTGGAAGGTGGATAACGCCCTCCAATCG8820.13676157278909876No Hit
GGCCCTGATGGGTGACTTCGCAGGCGTAGACTTTGTGTTTCTCGTAGTCTGCTTTGCTCAGCGTCAGGGTGCTG8810.13660651431654877No Hit
GTACTTTGGCCTCTCTGGGATAGAAGTTATTCAGCAGGCACACAACAGAGGCAGTTCCAGATTTCAACTGCTCAT8470.13133452624984882No Hit
GCTTTGCTCAGCGTCAGGGTGCTGCTGAGGCTGTAGGTGCTGTCCTTGCT8470.13133452624984882No Hit
GATTTCACTCTCACCATCAGCAGCCTGCAGGCTGAGGATGTGGCAGTTTATTATTGTCACCAATATTATAGTGCT8450.13102440930474882No Hit
ATAGTGCTCCGTGGACGTTCGGCCAAGGGACCAAGGTGGAAATCAAACGA8440.1308693508321988No Hit
GTCCTTGCTGTCCTGCTCTGTGACACTCTCCTGGGAGTTACCCGATTGGAGGGCGTTATCCACCTTCCACTGTA8350.12947382457924883No Hit
GGCCACCATCAACTGCAAGTCCAGCCGGAGTCTCTTATACACCTCCGACA8110.12575242123804886No Hit
CATTTATTGGGCATCCACCCGGGAATCCGGGGTCCCTGACCGATTCAGTGGCAGCGGGTCTGGGACAGATTTCA8000.12404677803999889No Hit
GGCAGGGGCAGCAAGATGGTGTTGCAGACCCAGGTCTTCATTTCTCTGTT7960.12342654414979888No Hit
GTCACATCCTGGCTGGGATTCGTGTAGTGCTTCACGTGGCATGTCACGGA7860.12187595942429891No Hit
GTTGAAGCTCTTTGTGACGGGCGAGCTCAGGCCCTGATGGGTGACTTCGC7750.12017031622624892No Hit
GGGTAGGTCCCCTGTGGAAAAAGGGTCAGAGGCCAAAGGATGGGAGGGGG7700.11939502386349891No Hit
GTGTGAGGTGCAGCTGGTGGAGTCTGGGGGAGGCTTGGTAAAGCCGGGGGGGTCCCTTAGACTCTCCTGTGCAGC7630.11830961455564894No Hit
GTGGAAAAAGGGTCAGAGGCCAAAGGATGGGAGGGGGTCAGGCTGGAACTGAGGAGCAGGTGGGGGCACTTCTCC7620.11815455608309892No Hit
ATTCAGTGGCAGCGGGTCTGGGACAGATTTCACTCTCACCATCAGCAGCC7610.11799949761054894No Hit
GTTGTGTGCCTGCTGAATAACTTCTATCCCAGAGAGGCCAAAGTACAGTG7600.11784443913799894No Hit
GGACTGAACACAGAGGACTCACCATGGAGTTTGGGCTGAGCTGGATTTTC7550.11706914677524895No Hit
GAGCAGGACAGCAAGGACAGCACCTACAGCCTCAGCAGCACCCTGACGCT7420.11505338663209896No Hit
CCCCTGTTGAAGCTCTTTGTGACGGGCGAGCTCAGGCCCTGATGGGTGACTTCGCAGGCGTAGACTTTGTGTTT7370.11427809426934898No Hit
CCCTTAGACTCTCCTGTGCAGCCTCTGGACTCACTTTCAGTAATGTCTGG7310.11334774343404896No Hit
GTCAGGGACCCCGGATTCCCGGGTGGATGCCCAATAAATGAGCACCTTAGGAGGCTGTCCTGGTTTCTGCTGCAA7260.112572451071299No Hit
GGATCTCTGGTGCCTACGGGGACGTCGTGATGACCCAGTCTCCAGACTCTCTGGCTGTGTCTCTGGGCGAGCGGG7220.11195221718109899No Hit
ACCTTCCACTGTACTTTGGCCTCTCTGGGATAGAAGTTATTCAGCAGGCA7210.11179715870854899No Hit
GAGTTTATTCAGGGGTGGGGACAGGCGGGTGGCTCAGTAGCAGGTGCCGT7170.111176924818349No Hit
GCACACAACAGAGGCAGTTCCAGATTTCAACTGCTCATCAGATGGCGGGA7170.111176924818349No Hit
GGTGAAAGATGAGCTGGAGGACCGCAATAGGGGTAGGTCCCCTGTGGAAA7130.110556690928149No Hit
GAACTGAGGAGCAGGTGGGGGCACTTCTCCCTCTAACACTCTCCCCTGTT7060.10947128162029902No Hit
GTGCAGCGACAGTCGGGGGTGGCAGCATGGGGGAGGTGGGGGAACTGGGC7030.10900610620264901No Hit
CCGCAATAGGGGTAGGTCCCCTGTGGAAAAAGGGTCAGAGGCCAAAGGAT7030.10900610620264901No Hit
GTTCAAGGACCACCTGAGCGTGACCTCTGTGGCTGCTACAGCGTGTCCAG7010.10869598925754903No Hit
GTCCTGGTTTCTGCTGCAACCAAGCCAAGTAGTTCCTGTTGTCGGAGGTG6960.10792069689479902No Hit
GCGTTATCCACCTTCCACTGTACTTTGGCCTCTCTGGGATAGAAGTTATTCAGCAGGCACACAACAGAGGCAGTT6650.10311388424574908No Hit
GTGTACAGGTCCCCGGAGGCATCCTGGCTAGGTGGGAAGTTTCTGGCGGT6590.10218353341044908No Hit
GTGTATAAGAGACTCCGGCTGGACTTGCAGTTGATGGTGGCCCGCTCGCC6580.10202847493789907No Hit
GGCTGAGGATGTGGCAGTTTATTATTGTCACCAATATTATAGTGCTCCGT6570.10187341646534909No Hit
GGACCAAGGTGGAAATCAAACGAACTGTGGCTGCACCATCTGTCTTCATC6550.10156329952024908No Hit
GATGAAGACAGATGGTGCAGCCACAGTTCGTTTGATTTCCACCTTGGTCC6520.10109812410259908No Hit
CTTCTATCCCAGAGAGGCCAAAGTACAGTGGAAGGTGGATAACGCCCTCCAATCGGGTAACTCCCAGGAGAGTGT6480.1004778902123991No Hit
GAGCAAAGCAGACTACGAGAAACACAAAGTCTACGCCTGCGAAGTCACCC6450.1000127147947491No Hit

[OK]Adapter Content

Adapter graph

Produced by FastQC (version 0.11.9)