FastQCFastQC Report
Wed 27 Apr 2022
EGAF00004684462

Summary

[OK]Basic Statistics

MeasureValue
FilenameEGAF00004684462
File typeConventional base calls
EncodingSanger / Illumina 1.9
Total Sequences689869
Sequences flagged as poor quality0
Sequence length20-76
%GC53

[OK]Per base sequence quality

Per base quality graph

[OK]Per tile sequence quality

Per tile quality graph

[OK]Per sequence quality scores

Per Sequence quality graph

[FAIL]Per base sequence content

Per base sequence content

[WARN]Per sequence GC content

Per sequence GC content graph

[OK]Per base N content

N content graph

[WARN]Sequence Length Distribution

Sequence length distribution

[FAIL]Sequence Duplication Levels

Duplication level graph

[WARN]Overrepresented sequences

SequenceCountPercentagePossible Source
CTCCTGGGAGTTACCCGATTGGAGGGCGTTATCCACCTTCCACTGTACTTTGGCCTCTCTGGGATAGAAGTTATT25380.36789593386570496No Hit
CTCTAACACTCTCCCCTGTTGAAGCTCTTTGTGACGGGCGAACTCAGGCC24290.3520958326870754No Hit
GATCTATGGTGTGTCCAATTTACAAACTGGGGTCCCAACAAGGTTCAGTG23880.3461526753630037No Hit
CCATAGATCAGGACGTGAGGGGCTTTCCCTGGTTTCTGTTGATACCAATT18960.27483478747414364No Hit
GGCTGGAACTGAGGAGCAGGTGGGGGCACTTCTCCCTCTAACACTCTCCC16260.23569692216928143No Hit
GGCGTAGACTTTGTGTTTCTCGTAGTCTGCTTTGCTCAGCGTCAGGGTGC16210.23497214688585805No Hit
CTCCCAGGAGAGTGTCACAGAGCAGGACAGCAAGGACAGCACCTACAGCC15840.22960880978852508No Hit
GTCTCAGTCAGGACACAGCATGGACATGAGGGTCCCCGCTCAGCTCCTGGGGCTCCTGCTACTCTGGCTCCGAGG15610.22627484348477755No Hit
GAGTTACAACACCCCGCTCACTTTCGGCGGAGGGACCAAGGTTGAGATCAAACGAACTGTGGCTGCACCAT14710.2132288883831568No Hit
GTTACAACACCCCGCTCACTTTCGGCGGAGGGACCAAGGTTGAGATCAAA14290.20714077600240047No Hit
GTCTACGCCTGCGAAGTCACCCATCAGGGCCTGAGTTCGCCCGTCACAAA14000.20293707935854485No Hit
CAATAGGGGTAGGTCCCCTGTGGAAAAAGGGTCAGAGGCCAAAGGATGGGAGGGGGTCAGGCTGGAACTGAGGAG13630.19757374226121194No Hit
GGATGGGAGGGGGTCAGGCTGGAACTGAGGAGCAGGTGGGGGCACTTCTCCCTCTAACACTCTCCCCTGTTGAAG13140.19047094448366284No Hit
CAACAGAGGCAGTTCCAGATTTCAACTGCTCATCAGATGGCGGGAAGATG12930.1874268882932847No Hit
GTCCTGCTCTGTGACACTCTCCTGGGAGTTACCCGATTGGAGGGCGTTATCCACCTTCCACTGTACTTTGGCCT12210.17699012421198806No Hit
GCGTCAGGGTGCTGCTGAGGCTGTAGGTGCTGTCCTTGCTGTCCTGCTCT11950.1732212927381865No Hit
GTACAGTGGAAGGTGGATAACGCCCTCCAATCGGGTAACTCCCAGGAGAG11920.1727864275681325No Hit
GCATGGACATGAGGGTCCCCGCTCAGCTCCTGGGGCTCCTGCTACTCTGG10820.15684137133281825No Hit
GTTGTAACTCTGCTGACAATAATAAGTTGCAAAATCTTCAGGTTGCAGACTGCTGATGGTGAGCGTGAACT10800.1565514612194489No Hit
CCACTGTACTTTGGCCTCTCTGGGATAGAAGTTATTCAGCAGGCACACAACAGAGGCAGTTCCAGATTTCAACTG10610.15379731514244008No Hit
GTCTTCATCTTCCCGCCATCTGATGAGCAGTTGAAATCTGGAACTGCCTCTGTTGTGTGCCTGCTGAATAACTT10420.15104316906543125No Hit
GTGTTGTAACTCTGCTGACAATAATAAGTTGCAAAATCTTCAGGTTGCAGACTGCTGATGGTGAGCGTGAACT10310.14944866344189983No Hit
CTCTGGGATAGAAGTTATTCAGCAGGCACACAACAGAGGCAGTTCCAGAT10080.1461146971381523No Hit
GTAGGAGACAGAGTCACCATCACTTGCCGGGCAAGTCAGAGTATCAGCAACTTTTTAAATTGGTATCAACAGAAA10000.14495505668467493No Hit
GAGCAGGACAGCAAGGACAGCACCTACAGCCTCAGCAGCACCCTGACGCT9980.14466514657130558No Hit
GAGTTCACGCTCACCATCAGCAGTCTGCAACCTGAAGATTTTGCAACTTATTATTGTCAGCAGAGTTACAACACC9890.1433605510611435No Hit
GTGGCTGCACCATCTGTCTTCATCTTCCCGCCATCTGATGAGCAGTTGAAATCTGGAACTGCCTCTGTTGTGTGC9680.1403164948707653No Hit
TCTATGGTGTGTCCAATTTACAAACTGGGGTCCCAACAAGGTTCAGTGGCAGTGGATCTGGGACAGAGTTCACG9470.13727243868038716No Hit
CCATCAGCAGTCTGCAACCTGAAGATTTTGCAACTTATTATTGTCAGCAG9390.13611279822690975No Hit
GGCTGTAGGTGCTGTCCTTGCTGTCCTGCTCTGTGACACTCTCCTGGGAGTTACCCGATTGGAGGGCGTTATCCA9360.13567793305685572No Hit
GTGCAGCCACAGTTCGTTTGATCTCAACCTTGGTCCCTCCGCCGAAAGTG9180.13306874203653157No Hit
CTTATACACATCTCCGAGCCCACGAGACTCCTGAGCATCTCGTATGCCGT9100.1319091015830542No Hit
GGCCAAAGGATGGGAGGGGGTCAGGCTGGAACTGAGGAGCAGGTGGGGGCACTTCTCCCTCTAACACTCTCCC9080.13161919146968482No Hit
CAGCATGGACATGAGGGTCCCCGCTCAGCTCCTGGGGCTCCTGCTACTCTGGCTCCGAGGCGCCCGATGTGACAT9000.13045955101620743No Hit
CTGCTACTCTGGCTCCGAGGCGCCCGATGTGACATCCAGATGACCCAGTC8820.12785035999588326No Hit
ACCTTGGTCCCTCCGCCGAAAGTGAGCGGGGTGTTGTAACTCTGCTGACA8720.12640080942903653No Hit
GGACACAGCATGGACATGAGGGTCCCCGCTCAGCTCCTGGGGCTCCTGCT8710.12625585437235184No Hit
GTCTCCATCTTCCCTGTCTGCTTCTGTAGGAGACAGAGTCACCATCACTTGCCGGGCAAGTCAGAGTATCAGCAA8570.1242264835787664No Hit
ATCCCAGAGAGGCCAAAGTACAGTGGAAGGTGGATAACGCCCTCCAATCG8310.12045765210496484No Hit
GATGAAGACAGATGGTGCAGCCACAGTTCGTTTGATCTCAACCTTGGTCC8160.11828332625469475No Hit
GCTTTGCTCAGCGTCAGGGTGCTGCTGAGGCTGTAGGTGCTGTCCTTGCT8040.11654386557447864No Hit
GACCAAGGTTGAGATCAAACGAACTGTGGCTGCACCATCTGTCTTCATCT7990.11581909029105526No Hit
GTCCCAGATCCACTGCCACTGAACCTTGTTGGGACCCCAGTTTGTAAATT7720.11190530376056904No Hit
GTTGTGTGCCTGCTGAATAACTTCTATCCCAGAGAGGCCAAAGTACAGTG7420.10755665206002879No Hit
GAGTTTATTCAGGGGTGGGGACAGGCGGGTGGCTCAGTAGCAGGTGCCGT7390.10712178688997477No Hit
ATCTGGGACAGAGTTCACGCTCACCATCAGCAGTCTGCAACCTGAAGATT7370.1068318767766054No Hit
GTATGCTGGTCACAGCGAAGGTGGTGGTGCCCTGGCTGGGCTCCTGCCGG7330.10625205654986672No Hit
GTCCTTGCTGTCCTGCTCTGTGACACTCTCCTGGGAGTTACCCGATTGGAGGGCGTTATCCACCTTCCACTGTA7320.10610710149318203No Hit
CCATCTTCCCTGTCTGCTTCTGTAGGAGACAGAGTCACCATCACTTGCCG7320.10610710149318203No Hit
CACCATAGATCAGGACGTGAGGGGCTTTCCCTGGTTTCTGTTGATACCAATTTAAAAAGTTGCTGATACTCTGA7290.10567223632312801No Hit
CTGCTGATGGTGAGCGTGAACTCTGTCCCAGATCCACTGCCACTGAACCT7170.10393277564291192No Hit
GTTGAAGCTCTTTGTGACGGGCGAACTCAGGCCCTGATGGGTGACTTCGC7080.10262818013274985No Hit
GTTCAGTGGCAGTGGATCTGGGACAGAGTTCACGCTCACCATCAGCAGTC7070.10248322507606517No Hit
GCTCCTGGGGCTCCTGCTACTCTGGCTCCGAGGCGCCCGATGTGACATCCAGATGACCCAGTCTCCATCTTCC6980.1011786295659031No Hit

[OK]Adapter Content

Adapter graph

Produced by FastQC (version 0.11.9)